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| griddap | Subset | tabledap | Make A Graph | wms | files | Accessible | Title | Summary | FGDC | ISO 19115 | Info | Background Info | RSS | Institution | Dataset ID | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_628993.subset | https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_628993 | https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_628993.graph | https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_628993/ | public | [Incubation in diffuse flow vent fluids - Crab Spa] - Results from shipboard high-pressure incubations of diffuse flow vent fluids collected from the Crab Spa and Alvinella sites at East Pacific Rise during the AT26-10 expedition, Jan. 2014 (Microbial Communities at Deep-Sea Vents project) (An Integrated Study of Energy Metabolism, Carbon Fixation, and Colonization Mechanisms in Chemosynthetic Microbial Communities at Deep-Sea Vents) | This dataset includes results from shipboard high-pressure incubations of\ndiffuse flow vent fluids collected from the Crab Spa (9.8398\\u00ba N,\n104.2913\\u00ba W) and Alvinella (9.8398\\u00ba N, 104.2915\\u00ba W) sites at\nEast Pacific Rise during the AT26-10 oceanographic expedition in January 2014.\nReported parameters include dates and time elapsed, flow rate, temperature,\npressure, and pH, and concentrations of NO3, NH4, H2, H2S, CH4.\n \nVent fluids used in shipboard incubations were corrected from diffuse flow\nvent sites at the East Pacific Rise (2503 m): Crab Spa (9.8398\\u00ba N,\n104.2913\\u00ba W) and Alvinella (9.8398\\u00ba N, 104.2915\\u00ba W) (see\ndescription in McNichol et al. [2016]). Fluids were collected using isobaric\ngas-tight samplers [Seewald et al., 2002] prior to their transfer to the\nshipboard continuous culture system [Foustoukos and Perez-Rodriguez, 2015].\nHere, high-pressure incubations (250 bars) were conducted at mesophilic (30\n\\u00baC) and thermophilic (50 \\u00baC) conditions to constrain the function\nand metabolic rates of denitrifying and DNRA microbial communities residing at\nCrab Spa and Alvinella, respectively. To enhance the activity of nitrate-\nrespiring anaerobic bacteria, an NO3- (5 mm) and H2(aq) (1.30 mM)-enriched\nmedium was introduced in the high-pressure incubations under strictly\nanaerobic conditions. Dissolved HCO3- (7.3 mm, 13C labeled) was used as added\ncarbon source. Vent fluids were introduced at a flow rate of 0.042 mL/min,\nwhile growth medium was added at a rate of 0.0042 mL/min. The two sets of\nexperiments were performed for 356 (Crab Spa) and 50 hours (Alvinella). Direct\ncell counts were conducted by staining cells with 0.1% acridine orange and\ncounting them with a fluorescence microscope. 15N/14N isotopic analysis of the\nNO3-, NH4+ and biomass were conducted with a Thermo Scientific Delta VPlus\nmass spectrometer and CE Instruments NA 2500 series elemental analyzer (EA).\n \nReferences:\n \nFoustoukos, D., and I. Perez-Rodriguez (2015), A continuous culture system for\nassessing microbial activities in the piezosphere, Applied and Environmental\nMicrobiology, 81(19), 6850-6856.\n \nMcNichol, J., S. P. Sylva, F. Thomas, C. D. Taylor, S. M. Sievert, and J. S.\nSeewald (2016), Assessing microbial processes in deep-sea hydrothermal systems\nby incubation at in situ temperature and pressure, Deep Sea Research Part I:\nOceanographic Research Papers, 115, 221-232.\n \nSeewald, J. S., K. W. Doherty, T. R. Hammar, and S. P. Liberatore (2002), A\nnew gas-tight isobaric sampler for hydrothermal fluids, Deep-Sea Research,\nPart I: Oceanographic Research Papers, 49(1), 189-196.\n\ncdm_data_type = Other\nVARIABLES:\ndescription (unitless)\n... (15 more variables)\n | https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_628993_iso19115.xml | https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_628993/index.htmlTable | https://www.bco-dmo.org/dataset/628993
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