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log in [16S microbiome data for artificial reef sponges and seawater] - 16S microbiome metadata collected from shallow artificial reef sponges and seawater in the Florida Keys, USA from Apr 2021 to Aug 2021 (Collaborative Research: Investigations into microbially mediated ecological diversification in sponges) Sponges are a dominant component of coral reefs worldwide and in the Caribbean, where their biomass exceeds that of reef-building corals. For almost a quarter century, the success of sponges in the Caribbean has been linked to their filter-feeding ability. However, recent work demonstrated that coexisting sponges on Caribbean reefs host unique communities of bacteria that might allow sponges to access multiple pools of nutrients that are not available to other organisms. In this project, the investigators will test the hypothesis that ecologically dominant sponge species in the Caribbean have unique metabolic strategies that are mediated by their associations with microbes that live within the sponge body.\n\nIn this dataset, we present the 16S rRNA microbiome NCBI accession and sample collection metadata for an artificial reef experiment where sponges of 10 species were placed on this temporary reef from April to August of 2021 and sampled using VacuSIP. VacuSIP methods capture incurrent (In) and excurrent (Ex) water from each sponge specimen. Incurrent represents the bacteria that are available for the sponge to consume via filter feeding and excurrent represents the bacteria that remain once sponges have consumed their preferred taxa. Additionally, we have provided microbiome metadata for the host sponges for several of these paired In/Ex samples. See the related dataset, NCBI Bioproject PRJNA1179970, for all sequence data. Microbiome data was generated using protocols from the Earth Microbiome project and sequencing was conducted on an Illumina MiSeq at Middle Tennessee State University. The data available at NCBI represents raw sequencing data, and no quality checks or sequencing filtering has been done on the uploaded sequences.\n\ncdm_data_type = Other\nVARIABLES:\ncollection_date (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\naccession (unitless)\nsample_name (unitless)\nspecies_ab (unitless)\nsample_type (unitless)\norganism (unitless)\n... (6 more variables)\n BCO-DMO bcodmo_dataset_953999_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_928039_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_928039_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_928039_v1/ public [Controlled laboratory study using model organisms Micromonas commoda RCC 299 and Ruegeria pomeroyi DSS-3] - Bacterial transcriptional response to picoeukaryote Micromonas commoda (Effects of Climate Change Variables on Microbial Autotroph-Heterotroph Carbon Flux) Marine biogeochemical cycles are built on interactions between surface ocean microbes, particularly those connecting phytoplankton primary producers to heterotrophic bacteria. However, direct influences of bacteria on phytoplankton physiology are poorly known. In this study, three marine bacteria (Ruegeria pomeroyi DSS-3, Stenotrophomonas sp. SKA14, and Polaribacter dokdonensis MED152) were co-cultured with green alga Micromonas commoda, and the phytoplankter's transcriptome was studied by RNASeq. The presence of each bacterium invoked transcriptomic remodeling by M. commoda after 8 h in co-culture. Some aspects of the algal transcriptomic response were conserved across all three bacteria, while others were restricted to a single bacterium. M. commoda had both rapid and extensive responses to heterotrophic bacteria.\n\ncdm_data_type = Other\nVARIABLES:\nBottle_ID (unitless)\nTreatment (unitless)\nTime_h (hour (h))\nMicromonas_cells_ml (cells per milliliter (cell/ml))\nBacteria_cells_ml (cells per milliliter (cell/ml))\nNH4_uM (micromolar (uM))\nNO3_uM (micromolar (uM))\nPO4_uM (micromolar (uM))\nNCBI_Sample_ID (unitless)\nAccession (unitless)\nBioProject (unitless)\nOrganism (unitless)\nTaxonomy_ID (unitless)\nDescription (unitless)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_928039_v1/index.htmlTable https://www.bco-dmo.org/dataset/928039 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_928039_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_928039_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_928039_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_762511 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_762511.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_762511/ public [Coral colony sequece accessions: Hidden resilience to recurrent bleaching] - RNA sequence accession numbers for coral colonies that displayed a strong bleaching phenotype at Ofu Island, American Samoa between 2015 and 2016. (Ecological, evolutionary and physiological responses of corals to a mass bleaching event in American Samoa) RNA sequence accession numbers for coral colonies that displayed a strong bleaching phenotype at Ofu Island, American Samoa between 2015 and 2016. This dataset includes accession numbers for 36 RNAseq libraries housed at The National Center for Biotechnology Information (NCBI).\n\ncdm_data_type = Other\nVARIABLES:\nsample (unitless)\ncolony (unitless)\nspecies (uniless)\ndate (unitless)\nyear (unitless)\nmonth (unitless)\nbleaching_status (percent)\nAccession (unitless)\nAccession_link (unitless)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_762511/index.htmlTable https://www.bco-dmo.org/dataset/762511 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_762511.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_762511&showErrors=false&email= BCO-DMO bcodmo_dataset_762511
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_762497.subset https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_762497 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_762497.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_762497/ public [Coral colony sequence accessions: The genomics of recovery from coral bleaching] - Coral colony genetic sequence accession numbers for samples collected from the lagoon environment of Ofu Island, American Samoa between 2011 and 2015. (Ecological, evolutionary and physiological responses of corals to a mass bleaching event in American Samoa) Coral colony genetic sequence accessions for samples collected from the lagoon environment of Ofu Island in the National Park of American Samoa between 2011 and 2015.\n\ncdm_data_type = Other\nVARIABLES:\nsample (unitless)\ndate (unitless)\ncolony (unitless)\nbleaching_status (percent (%))\naccession (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\nspecies (unitless)\naccession_link (untiless)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_762497_fgdc.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_762497/index.htmlTable https://www.bco-dmo.org/dataset/762497 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_762497.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_762497&showErrors=false&email= BCO-DMO bcodmo_dataset_762497
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_768550.subset https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_768550 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_768550.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_768550/ public [DYEatom: Metatranscriptome accessions and assembled contigs] - DYEatom Metatranscriptome metadata from RV/Point Sur cruise PS1312 in the Monterey Bay area, June-July 2013 (Linking physiological and molecular aspects of diatom silicification in field populations) Metadata for assembled contigs and ORFS from metatranscriptome analysis from CTD casts in the Monterey Bay area on RV/Point Sur cruise PS1312, June-July 2013. Assembled contigs files are also available; see Supplemental Files.\n\ncdm_data_type = Other\nVARIABLES:\nBioProject_type (Bio Project Type, unitless)\nBioProject_id (Bio Project Id, unitless)\nBioSample (Bio Sample, unitless)\nSample_name (unitless)\nSRA_id (unitless)\nPackage_type (unitless)\nversion (unitless)\nAccession (unitless)\nID (unitless)\ncruise_id (unitless)\nCTD_cast (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\nDate_collection (unitless)\nstation (unitless)\ndepth (m)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_768550_fgdc.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_768550/index.htmlTable https://www.bco-dmo.org/dataset/768550 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_768550.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_768550&showErrors=false&email= BCO-DMO bcodmo_dataset_768550
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_817298 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_817298.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_817298/ public [Hurricane Harvey Coral Gene Expression] - Coral gene expression Sequence Read Archive (SRA) accession numbers and information for samples collected at the Flower Garden Banks National Marine Sanctuary in the Gulf of Mexico in September and October of 2017 to capture effects of Hurricane Harvey (RAPID: Collaborative Research: Impact of freshwater runoff from Hurricane Harvey on coral reef benthic organisms and associated microbial communities) To capture the immediate effects of storm-driven freshwater runoff on coral and symbiont physiology, we leveraged the heavy rainfall associated with Hurricane Harvey in late August 2017 by sampling FGB coral gene expression at two time points: September 2017, when surface water salinity was reduced (\\u223c34 ppt); and 1 month later when salinity had returned to typical levels (\\u223c36 ppt in October 2017). \\r\\n\\r\\nThis dataset includes Sequence Read Archive (SRA) and BioSample accessions under BioProject PRJNA552981 at The National Center for Biotechnology Information.  It also contains sample information and species names for samples collected the east and west banks of the Flower Garden Banks National Marine Sanctuary (FGBNMS) at 80ft.\\r\\n\\r\\nThese data were published in Wright et al. (2019).\n\ncdm_data_type = Other\nVARIABLES:\nSample_Name (unitless)\ntime2 (Time, unitless)\nBank (unitless)\nBuoy (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\nSpecies (unitless)\ndepth (m)\nSRA (unitless)\nAccession (unitless)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_817298_fgdc.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_817298/index.htmlTable https://www.bco-dmo.org/dataset/817298 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_817298.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_817298&showErrors=false&email= BCO-DMO bcodmo_dataset_817298
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_756997.subset https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_756997 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_756997.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_756997/ public [Microbial community composition of the Cinder Cones Cold Seep] - Microbial community composition from 16s V4 region amplicon sequencing of the methane Seep at the Cinder Cones Cold Seep site, Nov 2016 (EAGER: Elucidating the Antarctic Methane Cycle at the Cinder Cones Reducing Habitat) This dataset includes microbial community composition from 16s V4 region amplicon sequencing on 151 marine sediment community samples collected from the Cinder Cones Cold Seep site [-77.8, 166.666] in the Ross Sea region, Antarctica in November 2016. Data are uploaded to the NCBI Sequence Read Archive under submission SUB2655615 [https://www.ncbi.nlm.nih.gov/bioproject/PRJNA387720] with a subset of the data from that archive originating from this project.\n\ncdm_data_type = Other\nVARIABLES:\naccession (unitless)\nsample_name (unitless)\norganism (unitless)\nhost (unitless)\ntime (Collection Date, seconds since 1970-01-01T00:00:00Z)\ngeo_loc_name (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\nReplicate (unitless)\nSediment_Depth_cm (centimeters)\nHabitat (unitless)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_756997_fgdc.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_756997/index.htmlTable https://www.bco-dmo.org/dataset/756997 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_756997.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_756997&showErrors=false&email= BCO-DMO bcodmo_dataset_756997
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_817436 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_817436.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_817436/ public [Sponge-Associated Microbial Communities (via 16S-V4 rRNA amplicon  sequencing) Following Storm-Driven Flooding] - SRA accessions and collection information for 16S-V4 rRNA amplicon data from invertebrates sampled at Flower Garden Banks National Marine Sanctuary, Gulf of Mexico following Tax Day Flooding (2016), Hurricane Harvey (2017), and a no flooding year (2018) (RAPID: Collaborative Research: Impact of freshwater runoff from Hurricane Harvey on coral reef benthic organisms and associated microbial communities) To document the effects of storm-driven freshwater runoff on\\u00a0sponge-\nassociated microbiomes, we leveraged the heavy rainfall\\u00a0associated with\nTax Day Flooding (July 2016) and Hurricane Harvey\\u00a0(August 2017) to\ncharacterize sponge-associated bacterial communities\\u00a0at five time points:\nin July 2016 (at detection of the mortality\\u00a0event), one month after the\nmortality event (August 2016), immediately\\u00a0after Hurricane Harvey\n(September 2017), one month after Hurricane\\u00a0Harvey (October 2017), and\napproximately one year following Hurricane\\u00a0Harvey (October 2018).\n \nThese data contain Sequence Read Archive (SRA) and BioSample accession numbers\nassociated with BioProject\\u00a0PRJNA605902\n(see\\u00a0[https://www.ncbi.nlm.nih.gov/bioproject/605902](\\\\https://www.ncbi.nlm.nih.gov/bioproject/605902\\\\))\\u00a0at\nThe National Center for Biotechnology Information.\n\ncdm_data_type = Other\nVARIABLES:\nSample_Name (unitless)\nCollection_Date_Start (unitless)\nCollection_Date_End (unitless)\nDepth_min (Depth, meters (m))\ndepth (m)\nBank (unitless)\nSpecies (unitless)\nSRA (unitless)\nAccession (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_817436_fgdc.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_817436/index.htmlTable https://www.bco-dmo.org/dataset/817436 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_817436.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_817436&showErrors=false&email= BCO-DMO bcodmo_dataset_817436
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_916134_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_916134_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_916134_v1/ public [Substrate-specific metabolic responses of model marine bacteria] - Metadata for transcriptomic expression data from cultures of Ruegeria pomeroyi DSS-3 and Alteromonas macleodii MIT1002 grown in defined culture media with either glucose, acetate, or a mix of both as carbon substrates (C-CoMP Model Bacteria Physiological Studies) This dataset includes metadata for transcriptomic expression data from cultures of Ruegeria pomeroyi DSS-3 and Alteromonas macleodii MIT1002. These model marine bacteria were grown in defined culture media with either glucose, acetate, or a mix of both as carbon substrates. The data are sampled so as to capture the metabolic differences the bacteria employ when catabolizing these different substrates and when switching between them. The raw RNA sequences (50 bp reads in fastq format) have been submitted to the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) under BioProject PRJNA972985 (https://www.ncbi.nlm.nih.gov/bioproject/972985).\n\ncdm_data_type = Other\nVARIABLES:\nSample_Name (unitless)\nAccession (unitless)\nBioProject (unitless)\nOrganism (unitless)\nStrain (unitless)\nTax_ID (unitless)\nGlucose_initial_concentration_uM (micromolar concentration)\nAcetate_initial_concentration_uM (micromolar concentration)\nSample_description (unitless)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_916134_v1/index.htmlTable https://www.bco-dmo.org/dataset/916134 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_916134_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_916134_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_916134_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_782301.subset https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_782301 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_782301.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_782301/ public [Synechococcus accessions] - NCBI accessions for raw genomic sequence data of 11 new isolates of marine Synechococcus from Naragansett Bay, July 2017 (Dimensions: Collaborative Research: Genetic, functional and phylogenetic diversity determines marine phytoplankton community responses to changing temperature and nutrients) NCBI accessions for raw genomic sequence data of 11 new isolates of marine Synechococcus from Naragansett Bay.\n\ncdm_data_type = Other\nVARIABLES:\nAccession (unitless)\nSample_Name (unitless)\nSPUID (unitless)\nOrganism (unitless)\nTax_ID (unitless)\nIsolate (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\ndepth (m)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_782301_fgdc.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_782301/index.htmlTable https://www.bco-dmo.org/dataset/782301 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_782301.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_782301&showErrors=false&email= BCO-DMO bcodmo_dataset_782301
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_739636.subset https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_739636 https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_739636/ public [Thaumarchaea transcriptomes] - Nitrosopelagicus brevis CN25 and U25 grown in nitrogen replete and deplete conditions, with subsequent transcriptome sequencing and identification. (Gene content, gene expression, and physiology in mesopelagic ammonia-oxidizing archaea) Nitrosopelagicus brevis CN25 and U25 were grown in nitrogen replete and deplete conditions, with subsequent transcriptome sequencing.\n\ncdm_data_type = Other\nVARIABLES:\nAccession (unitless)\nSample_Name (unitless)\nOrganism (unitless)\nTax_ID (unitless)\nStrain (unitless)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_739636/index.htmlTable https://www.bco-dmo.org/dataset/739636 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_739636.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_739636&showErrors=false&email= BCO-DMO bcodmo_dataset_739636
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_924786_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_924786_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_924786_v1/ public [Whole Genome Sequencing of Eelgrass Bodega and Tomales Bay] - Sample collection information and sequence accessions at the National Center for Biotechnology Information (NCBI) for whole genome sequencing of eelgrass (Zostera marina) collected at Bodega and Tomales Bay, CA, USA from July to September 2019 (Using genomics to link traits to ecosystem function in the eelgrass Zostera marina) This dataset includes sample collection information and sequence accessions at the National Center for Biotechnology Information (NCBI) for whole genome sequencing of eelgrass (Zostera marina) collected at Bodega and Tomales Bay, California, USA from July and September of 2019. Sequence Read Archive (SRA) Experiments and BioSamples can be accessed from the NCBI BioProject PRJNA887384 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA887384/).\n\nResults summary as described in Scheibelhut, et al. (2023): We examine genomic signals of selection in the eelgrass Zostera marina across temperature gradients in adjacent embayments. Although we find many genomic regions with signals of selection within each bay there is very little overlap in signals of selection at the SNP level, despite most polymorphisms being shared across bays. We do find overlap at the gene level, potentially suggesting multiple mutational pathways to the same phenotype. Using polygenic models we find that some sets of candidate SNPs are able to predict temperature across both bays, suggesting that small but parallel shifts in allele frequencies may be missed by independent genome scans. Together, these results highlight the continuous rather than binary nature of parallel evolution in polygenic traits and the complexity of evolutionary predictability.\n\ncdm_data_type = Other\nVARIABLES:\naccession (unitless)\nsample_name (unitless)\nbioproject_accession (unitless)\nSite (unitless)\norganism (unitless)\ncollection_date (unitless)\nisolation_source (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_924786_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_924786_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_924786_v1/index.htmlTable https://www.bco-dmo.org/dataset/924786 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_924786_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_924786_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_924786_v1

 
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