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BCO-DMO ERDDAP
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| griddap | Subset | tabledap | Make A Graph | wms | files | Accessible | Title | Summary | FGDC | ISO 19115 | Info | Background Info | RSS | Institution | Dataset ID | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_994735_v1 | https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_994735_v1.graph | https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_994735_v1/ | public | [flow cytometry] - Flow cytometric analysis of interaction between Alteromonas macleodii exudates and Prochlorococcus MIT9312 cells (Collaborative Research: Extracellular vesicles as vehicles for microbial interactions in marine Black Queen communities) | The cyanobacterium Prochlorococcus has a conspicuously reduced genome causing it to require help from co-existing organisms for survival under a variety of stressful conditions. In this work we used conditioned media experiments to demonstrate that exudates of the heterotrophic bacterium Alteromonas macleodii EZ55 facilitated the survival of Prochlorococcus MIT9312 batch co-cultures as they entered stationary phase. Microscopy revealed the presence of extracellular membrane vesicles and protein complexes in the exudates. When the exudates were stained with an amine-reactive dye, we were able to observe them interacting directly with Prochlorococcus cells by flow cytometry, where stained exudates (but not dye alone) added both green fluorescence and size (forward scatter) to Prochlorococcus cells.\n\ncdm_data_type = Other\nVARIABLES:\nFile (unitless)\nTreatment (unitless)\nAnalyzed_Events (unitless)\nFixed (unitless)\nStained (unitless)\nDilution (unitless)\nPrefiltration (unitless)\nFSC_Mean (arbitrary fluorescence units (AFU))\nGreen_Mean (arbitrary fluorescence units (AFU))\nRed_Mean (arbitrary fluorescence units (AFU))\nFSC_Median (arbitrary fluorescence units (AFU))\nGreen_Median (arbitrary fluorescence units (AFU))\nRed_Median (arbitrary fluorescence units (AFU))\nFSC_CV (arbitrary fluorescence units (AFU))\nGreen_CV (arbitrary fluorescence units (AFU))\nRed_CV (arbitrary fluorescence units (AFU))\n | https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_994735_v1/index.htmlTable | https://osprey.bco-dmo.org/dataset/994735
| https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_994735_v1.rss | https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_994735_v1&showErrors=false&email= | BCO-DMO | bcodmo_dataset_994735_v1 |