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| griddap | Subset | tabledap | Make A Graph | wms | files | Accessible | Title | Summary | FGDC | ISO 19115 | Info | Background Info | RSS | Institution | Dataset ID | |
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| log in | [G. huxleyi CCMP371 alkaline phosphatase activity] - Alkaline phosphatase activity for G. huxleyi CCMP371 grown under low N and low P N:P ratios in laboratory-based culture experiments (Collaborative Research: Defining the role of the pan genome in Emiliania huxleyi ecology and biogeography) | Gephyrocapsa huxleyi, a ubiquitous marine coccolithophore, plays a significant role in marine ecosystems and the global carbon cycle. Broadly, this project seeks to define the role of the pan-genome in G. huxleyi ecology and biogeography and provide insight into how this taxon's genotypic variability influences its biogeography and ecology, and ultimately how this genus may adapt to changing environmental conditions in the future ocean. This project leveraged the G. huxleyi pan-genome database to inform a series of additional laboratory-based culture experiments to relate genotype to phenotype in different strains under shifting response drivers, specifically nitrogen (N) and phosphorus (P). Here, we grew G. huxleyi CCMP371, a highly calcifying, axenic strain, under low N and low P conditions by modulating the dissolved inorganic nitrogen (DIN) to dissolved inorganic phosphorus (DIP) ratios. The CCMP371 experiment began as batch cultures until a maximum in vivo chlorophyll fluorescence was reached (based on a previous growth experiment; data contributed to BCO-DMO in this same project). At this point, an equal volume of culture was removed from triplicate flasks and replaced with an equal volume of modified L1/5-ASW, with an N:P of 0.5:1 (0.5 µM N and 1 µM P) for low N conditions. Likewise, an equal volume of culture was removed from triplicate flasks and replaced with an equal volume of L1/5-ASW, with an N:P of 125:1 (25 µM N and 0.2 µM P) for low P conditions. Dilutions continued until steady-state conditions, determined by microscopic cell counts, were reached. Once the low N and low P triplicate cultures reached steady state, samples were collected for a suite of physicochemical parameters, including alkaline phosphatase activity reported in this dataset. Alkaline phosphatase activity (APA) is a common physiological marker of P stress, where increased APA indicates a phenotypic shift toward the use of organic P sources. In this experiment, APA was used to confirm P-deficiency in the low P cultures relative to the low N cultures. These data were used to inform additional nutrient amendment experiments with G. huxleyi strains (reported elsewhere), and they can be used to better constrain existing tools, such as the Global Alkaline Phosphatase Activity Dataset (GAPAD).\n\ncdm_data_type = Other\nVARIABLES:\nG_huxleyi_ID (unitless)\nExperimental_Treatment (unitless)\nReplicate (unitless)\nAPA_nmolP_cell_hour (nmol per cell per hour)\n | BCO-DMO | bcodmo_dataset_998945_v1 |