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| griddap | Subset | tabledap | Make A Graph | wms | files | Accessible | Title | Summary | FGDC | ISO 19115 | Info | Background Info | RSS | Institution | Dataset ID | |
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| log in | [P_pools_EFB_cultures_WH8102] - Cell abundance, nutrient concentrations, and intracellular phosphorus pools in Synechococcus sp. WH8102 continuous (EFB) culture experiments (Collaborative Research: Assessing the role of polyphosphate production and cycling in marine ecosystem functioning) | This dataset contains measurements of fluorescence, cell abundance, phosphate concentrations, and intracellular phosphorus (P) pools from continuous culture experiments of Synechococcus sp. WH8102 conducted under controlled laboratory P treatments (High-P, Mid-P, and Low-P). Experiments were performed under steady-state conditions in exponentially fed batch (EFB) cultures (Fischer et al., 2014), with three biological replicates per treatment for four to five consecutive days. Intracellular P partitioning across major macromolecular pools, including ATP, DNA, RNA, free phosphate (Pi), phospholipids, and polyphosphate (polyP). Intracellular P pools were quantified using established biochemical and analytical methods appropriate for each fraction, enabling assessment of cellular P allocation under contrasting nutrient regimes. These data were generated to investigate how P availability constrains microbial physiology, cellular resource allocation, and population dynamics in marine cyanobacteria. The dataset provides a comprehensive view of physiological responses across organizational scales, linking P conditions and growth rates to intracellular P allocation and cell physiology in Synechococcus. Together, these measurements provide a resource for examining P-driven changes in cellular composition and nutrient use strategies in marine microbial systems.\n\ncdm_data_type = Other\nVARIABLES:\ntreatment (categorical (none))\nday (days)\nbottle (categorical (none))\nFLUO (RFU (relative fluorescence units))\ncell (cells per mL (cells / mL))\nSRP (µM (micromoles per liter))\nbiovolume (µm3 per cell)\ngrowth_rates_1_day (per day)\nATP_value_uM (µM (micromoles per liter))\nATP_value_fmol_um3 (fmol µm⁻³)\nATP_value_fmol_cell (fmol cell⁻¹)\nDNA_value_uM (µM (micromoles per liter))\nDNA_value_fmol_um3 (fmol P µm⁻³)\n... (16 more variables)\n | BCO-DMO | bcodmo_dataset_1005084_v1 |