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log in [EN584 Bulk PA FlaPS Rates] - Polysaccharide hydrolysis rates from bulk water and 3 µm retained fraction (particle-associated) incubations in the Northwest Atlantic aboard the R/V Endeavor cruise EN584 from Jun to Jul 2016 (Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean?) The potential of the seawater microbial community to hydrolyze six high-molecular-weight polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan, laminarin, pullulan, and xylan) was investigated at various sites and depths of the Northwest Atlantic. Nearshore waters close to Cape Hatteras/Cape Lookout, and a transect along ~36° N out to ~58° W were collected during the summer of 2016, aboard the R/V Endeavor cruise EN584. Hydrolysis of high molecular weight substrates to lower molecular weight hydrolysis products was measured using gel permeation chromatography with fluorescence detection, after the method of Arnosti (1996, 2003). This dataset includes polysaccharide hydrolysis rates to measure microbial enzyme activities from bulk water and 3 µm retained fraction (particle-associated) incubations.\n\ncdm_data_type = Other\nVARIABLES:\nDeployment (unitless)\nstn (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\nISO_DateTime_Local (unitless)\ntime (Iso_datetime_utc, seconds since 1970-01-01T00:00:00Z)\ncast_number (unitless)\ndepth_sequence (unitless)\ndepth (Depth_actual, m)\nsample_type (unitless)\namended_unamended (unitless)\nsubstrate (unitless)\ntimepoint (unitless)\ntime_elapsed_hr (hours)\nrep1_rate (nM*hr-1)\nrep2_rate (nM*hr-1)\nrep3_rate (nM*hr-1)\naverage (nM*hr-1)\nstd_dev (nM*hr-1)\n BCO-DMO bcodmo_dataset_986681_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_994900_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_994900_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_994900_v1/ public [EN638 LV Cell Counts] - Cell abundance in HMW-DOM amended mesocosm incubations from water collected in the Western North Atlantic during the research cruise EN638 in May 2019 aboard R/V Endeavor (A mechanistic microbial underpinning for the size-reactivity continuum of dissolved organic carbon degradation) Marine dissolved organic matter (DOM) is one of the largest actively-cycling reservoirs of organic carbon on the planet, and thus a major component of the global carbon cycle. The existence of a size-reactivity continuum of DOM - observations and measurements showing that high molecular weight (HMW) DOM tends to be younger and more reactive than lower MW DOM - has been demonstrated in laboratory and field investigations in different parts of the ocean. A mechanistic explanation for the greater reactivity of HMW DOM has been lacking, however.  \n \nHere we investigated the potential of seawater microbial communities from different water masses and under differing conditions of organic matter availability to hydrolyze six high-molecular-weight polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan, laminarin, pullulan, and xylan). Samples were taken from incubation experiments of DCM, OMZ, and bottom waters aboard R/V Endeavor (EN638), May 2019 in the Northern Atlantic.\n \nThis dataset includes the measurement of cell abundance from samples taken from mesocosm incubations after the addition of HMW DOM.\n\ncdm_data_type = Other\nVARIABLES:\nDeployment (unitless)\nStation (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\ntime_sampled_EDT (unitless)\ntime (Time_sampled_utc, seconds since 1970-01-01T00:00:00Z)\ncast_number (unitless)\ndepth_sequence (unitless)\ndepth (Depth_m, m)\nin_situ_temp (degrees Celsius)\nincub_temp (degrees Celsius)\nsample_type (unitless)\nunammended_ammended (unitless)\n... (4 more variables)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_994900_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_994900_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_994900_v1/index.htmlTable https://osprey.bco-dmo.org/dataset/994900 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_994900_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_994900_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_994900_v1
log in [Plate Rates Bulk TN362] - Peptidase and glucosidase activities from bulk water incubations from waters taken aboard the R/V Thomas G. Thompson in the Southern Indian Ocean during the research cruise TN362 in November and December, 2018 (A mechanistic microbial underpinning for the size-reactivity continuum of dissolved organic carbon degradation) Marine dissolved organic matter (DOM) is one of the largest actively-cycling reservoirs of organic carbon on the planet, and thus a major component of the global carbon cycle. The existence of a size-reactivity continuum of DOM - observations and measurements showing that HMW (high-molecular-weight) DOM tends to be younger and more reactive than lower MW (molecular-weight) DOM - has been demonstrated in laboratory and field investigations in different parts of the ocean. A mechanistic explanation for the greater reactivity of HMW DOM has been lacking, however. Here we investigated the potential of seawater microbial communities from different water masses to hydrolyze six high-molecular-weight polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan, laminarin, pullulan, and xylan).  \n \nThis dataset includes the measurement of peptidase and glucosidase activities from samples taken from bulk water incubations from various stations and depths aboard the R/V Thomas G. Thompson in the Southern Indian Ocean during the research cruise TN362 in November and December, 2018.\n\ncdm_data_type = Other\nVARIABLES:\ndeployment (unitless)\nStation (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\ndate (unitless)\ndepth_sequence (unitless)\ndepth (Depth_actual, m)\nsample_type (unitless)\nIncubation_temp (degrees Celsius)\nunamended_amended (unitless)\nsubstrate (unitless)\nt1_rate (nmol L-1 hr-1)\nt1_sd (nmol L-1 hr-1)\nt2_rate (nmol L-1 hr-1)\nt2_sd (nmol L-1 hr-1)\n... (15 more variables)\n BCO-DMO bcodmo_dataset_996093_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_1000862_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_1000862_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_1000862_v1/ public [Polysaccharide hydrolysis rates from Arctic fjord Kongsfjorden April 2018] - Measurement of polysaccharide hydrolysis rates from water samples collected from the Arctic fjord Kongsfjorden near Ny-Ålesund, Svalbard, during April 2018 (A mechanistic microbial underpinning for the size-reactivity continuum of dissolved organic carbon degradation) Marine dissolved organic matter (DOM) is one of the largest actively-cycling reservoirs of organic carbon on the planet, and thus a major component of the global carbon cycle. The existence of a size-reactivity continuum of DOM - observations and measurements showing that high molecular weight (HMW) DOM tends to be younger and more reactive than lower MW DOM - has been demonstrated in laboratory and field investigations in different parts of the ocean. A mechanistic explanation for the greater reactivity of HMW DOM has been lacking, however. Here we investigated the potential of seawater microbial communities from different water masses to hydrolyze six high-molecular-weight polysaccharides: arabinogalactan, chondroitin sulfate, fucoidan, laminarin, pullulan, and xylan.\n\nThis dataset includes measurement of polysaccharide hydrolase activities obtained from seawater incubations. Seawater samples were collected at various stations and depths in Kongsfjorden, an Arctic fjord near Ny-Ålesund, Svalbard, in April 2018.\n\ncdm_data_type = Other\nVARIABLES:\ndeployment (unitless)\nStation (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\ndate (unitless)\ndepth_sequence (unitless)\ndepth (Depth_actual, m)\nin_situ_Temp (degrees Celsius (°C))\nin_situ_Sal (ppt)\nin_situ_chl (Fluorescence units)\nsample_type (unitless)\nIncubation_temp (degrees Celsius (°C))\nunamended_amended (unitless)\nSub_sample_day (Days)\nsubstrate (unitless)\nrate_1 (nanomoles per liter per hour (nmol L-1 hr-1))\n... (4 more variables)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_1000862_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_1000862_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_1000862_v1/index.htmlTable https://osprey.bco-dmo.org/dataset/1000862 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_1000862_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_1000862_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_1000862_v1
log in [Polysaccharide Hydrolysis Rates TN362] - Polysaccharide hydrolysis rates from bulk water incubations from waters taken aboard the R/V Thomas G. Thompson in the Southern Indian Ocean during the research cruise TN362 from November and December, 2018 (A mechanistic microbial underpinning for the size-reactivity continuum of dissolved organic carbon degradation) Marine dissolved organic matter (DOM) is one of the largest actively-cycling reservoirs of organic carbon on the planet, and thus a major component of the global carbon cycle. The existence of a size-reactivity continuum of DOM - observations and measurements showing that HMW (high-molecular-weight) DOM tends to be younger and more reactive than lower MW (molecular-weight) DOM - has been demonstrated in laboratory and field investigations in different parts of the ocean. A mechanistic explanation for the greater reactivity of HMW DOM has been lacking, however. Here we investigated the potential of seawater microbial communities from different water masses to hydrolyze six high-molecular-weight polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan, laminarin, pullulan, and xylan).  \n \nThis dataset includes the measurement of polysaccharide hydrolase activities from samples taken from bulk water incubations from various stations and depths aboard the R/V Thomas G. Thompson in the Southern Indian Ocean during the research cruise TN362 in November and December, 2018.\n\ncdm_data_type = Other\nVARIABLES:\ndeployment (unitless)\nStation (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\ndate (unitless)\ndepth_sequence (unitless)\ndepth (Depth_actual, m)\nsample_type (unitless)\nIncubation_temp (degrees Celsius)\nunamended_amended (unitless)\nSub_sample_day (days)\nsubstrate (unitless)\nrate_x_nM_hr (nmol L-1 hr-1)\nrate_1_nM_hr (nmol L-1 hr-1)\nrate_2_nM_hr (nmol L-1 hr-1)\n... (9 more variables)\n BCO-DMO bcodmo_dataset_996032_v1

 
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