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| griddap | Subset | tabledap | Make A Graph | wms | files | Accessible | Title | Summary | FGDC | ISO 19115 | Info | Background Info | RSS | Institution | Dataset ID | |
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| log in | [Acropora cervicornis 16S rRNA sequence metadata: nutrient- and disease-exposed] - Acropora cervicornis 16S rRNA sequence metadata: nutrient- and disease-exposed from samples collected at Mote Marine Laboratory in situ nursery from June to July 2022 (Collaborative Research: Tracking the interacting roles of the environment, host genotype, and a novel Rickettsiales in coral disease susceptibility) | In the summer of 2022, 20 ramets each of the Acropora cervicornis genotypes ML-AC-36 and ML-AC-46 were collected from Mote's in situ coral nursery and subjected to three weeks of elevated nitrate, ammonium, and phosphate in the form of a slow-release fertilizer. The duration and concentration of this exposure were selected based on prior experiments conducted at Mote Marine Laboratory, which were found to alter microbial community profiles and growth rates in A. cervicornis (Klinges et al., 2022, 2023). During experiment, corals were held in 2-gallon aquaria with 5 corals per aquarium. As Mote Marine Laboratory's experimental aquarium system is plumbed into nearshore coastal water and thus has a higher nutrient load than the reef, an additional subset of 18 ramets of each genotype was collected from Mote's in situ coral nursery immediately prior to disease challenge to evaluate the impact of nearshore water on disease response. All ramets in the disease group were subjected to disease challenge in the form of a tissue homogenate produced from diseased fragments of random genotypes of A. cervicornis. Ramets in a comparative unexposed group were exposed to a homogenate produced from healthy fragments of random genotypes of A. cervicornis. We collected 190 samples for 16S rRNA sequencing, including prior to nutrient exposure, nutrient-exposed and -unexposed corals prior to disease challenge, and at several timepoints throughout disease exposure capturing both diseased and apparently healthy phenotypes. We additionally collected numerous samples for measurements of immune-related proteins. In brief, we found that disease challenge significantly impacted microbial communities, leading to significant differences in community composition between disease-exposed samples that developed disease, exposed samples that resisted disease, and healthy unexposed controls. The taxa Algicola, Halarcobacter, Saprospira, Shimia, Tenacibaculum, Thalassolituus, and Thalassotalea increased in abundance in samples that developed disease. Of the genotypes chosen for the experiment, one genotype was dominated by the putative parasite Aquarickettsia. We found that both genotypes were susceptible to disease, in contrast to previous results indicating that genotypes with low abundance of Aquarickettsia and relatively diverse microbiomes were disease-resistant. Raw sequence reads have been submitted to SRA under PRJNA1024453 with an embargo until publication.\n\ncdm_data_type = Other\nVARIABLES:\nNCBI_Project (unitless)\nsample_name (unitless)\n... (30 more variables)\n | BCO-DMO | bcodmo_dataset_924465_v1 | ||||||||||||
| log in | [Acropora cervicornis protein measurements: nutrient- and disease-exposed] - Acropora cervicornis protein measurements: nutrient- and disease-exposed from samples collected at Mote Marine Laboratory in situ nursery from June to July 2022 (Collaborative Research: Tracking the interacting roles of the environment, host genotype, and a novel Rickettsiales in coral disease susceptibility) | In the summer of 2022, 20 ramets each of the Acropora cervicornis genotypes ML-AC-36 and ML-AC-46 were collected from Mote's in situ coral nursery and subjected to three weeks of elevated nitrate, ammonium, and phosphate in the form of a slow-release fertilizer. The duration and concentration of this exposure were selected based on prior experiments conducted at Mote Marine Laboratory, which were found to alter microbial community profiles and growth rates in A. cervicornis (Klinges et al., 2022, 2023). During experiment, corals were held in 2-gallon aquaria with 5 corals per aquarium. As Mote Marine Laboratory's experimental aquarium system is plumbed into nearshore coastal water and thus has a higher nutrient load than the reef, an additional subset of 18 ramets of each genotype was collected from Mote's in situ coral nursery immediately prior to disease challenge to evaluate the impact of nearshore water on disease response. All ramets in the disease group were subjected to disease challenge in the form of a tissue homogenate produced from diseased fragments of random genotypes of A. cervicornis. Ramets in a comparative unexposed group were exposed to a homogenate produced from healthy fragments of random genotypes of A. cervicornis. To evaluate the effect of Aquarickettsia infection; nutrient enrichment; disease exposure; and the combination of these factors on coral immune function, a number of immune related proteins and antioxidants were measured using microplate assays from a total of 55 samples taken prior to disease exposure and following disease exposure as corals either resisted or developed disease. Total host protein and the antioxidant superoxide dismutase were found to be higher in healthy corals regardless of genotype or nutrient enrichment. Phenoloxidase and prophenoloxidase were higher in concentration in diseased samples compared to healthy samples. Disease-exposed but apparently healthy corals had higher superoxide dismutase, prophenoloxidase, and peroxidase activity than either healthy unexposed corals or diseased corals despite total protein concentrations that were lower than healthy unexposed corals.\n\ncdm_data_type = Other\nVARIABLES:\nFull_ID (unitless)\nDate (unitless)\nTP (mg)\nPOX (ΔAbs470nm)\nNormalized_POX (ΔAbs470nm/mg protein)\n... (17 more variables)\n | BCO-DMO | bcodmo_dataset_924466_v1 | ||||||||||||
| https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_754885.subset | https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_754885 | https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_754885.graph | https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_754885/ | public | [Incubation Data] - Vascular plant and microbial biomarkers of dissolved organic matter data from incubation experiments (Collaborative Research: Calibration and application of vascular plant and aqueous microbial biomarkers to examine transformations of dissolved organic matter) | Incubation experiments were conducted in the dark or using a dark/light cycle. Incubations conducted in the dark alone are classified as \\microbial, and incubations using a dark/light cycle are classified as \\coupled\\.\n\ncdm_data_type = Other\nVARIABLES:\nincubation_type (unitless)\nsite_type (unitless)\ntime_point (unitless)\ndays (days)\nhours (hours)\nExposure (hours)\nDose (MJ/m2)\nIrradiance (W/m2)\nDOC_mg_L (mg/L)\nDOC_mM (DOC M M, mM)\nFuc (nanomoles per liter (nmol/L))\nRha (nanomoles per liter (nmol/L))\nAra (nanomoles per liter (nmol/L))\nGal (nanomoles per liter (nmol/L))\nGlu (nanomoles per liter (nmol/L))\nMan (nanomoles per liter (nmol/L))\nXyl (nanomoles per liter (nmol/L))\nD_Asx (nanomoles per liter (nmol/L))\nL_Asx (nanomoles per liter (nmol/L))\nD_Glx (nanomoles per liter (nmol/L))\nL_Glx (nanomoles per liter (nmol/L))\nD_Ser (nanomoles per liter (nmol/L))\nL_Ser (nanomoles per liter (nmol/L))\nD_His (nanomoles per liter (nmol/L))\nL_His (nanomoles per liter (nmol/L))\n... (69 more variables)\n | https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_754885/index.htmlTable | https://www.bco-dmo.org/dataset/754885
| https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_754885.rss | https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_754885&showErrors=false&email= | BCO-DMO | bcodmo_dataset_754885 | ||||
| https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_933334_v1 | https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_933334_v1.graph | https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_933334_v1/ | public | [Summary data from the Heatwaves and Coral-Recovery Database (HeatCRD)] - Summary data from the Heatwaves and Coral-Recovery Database (HeatCRD) covering global coral reef sites from 1977-2020 (Thermal stress and differential recovery of coral reefs) | This dataset is a summary table of the Heatwaves and Coral-Recovery Database (HeatCRD) introduced in van Woesik and Kratochwill (2024). The HeatCRD is the most comprehensive reference on coral recovery following marine heatwaves and other disturbances, encompassing 29,205 data records spanning 44 years from 12,266 sites, 83 countries, and 160 data sources. These data provide essential information to coral-reef scientists and managers to best guide coral-reef conservation efforts at both local and regional scales.\nThe dataset includes metadata for coral reef sampling events, such as site descriptions, geographical coordinates, depth, distance to shore, exposure, turbidity, coral cover percentages, MPA descriptions, temperature measurements, windspeed, and thermal stress indicators over 23 years.\n\nSee van Woesik and Kratochwill (2024) https://doi.org/10.1038/s41597-024-03221-3 for more information.\n\ncdm_data_type = Other\nVARIABLES:\nSite_ID (unitless)\nSample_ID (unitless)\nlatitude (Latitude_degrees, degrees_north)\nlongitude (Longitude_degrees, degrees_east)\nOcean_Name (unitless)\nRealm_Name (unitless)\nEcoregion_Name (unitless)\nEcoregion_distance (degrees)\nCountry_Name (unitless)\nState_Island_Province_Name (unitless)\nLocation_Name (unitless)\nSite_Name (unitless)\nHabitat_Type (unitless)\nHabitat_Distance (degrees)\nDate_Day (unitless)\nDate_Month (unitless)\n... (59 more variables)\n | https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_933334_v1_fgdc.xml | https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_933334_v1_iso19115.xml | https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_933334_v1/index.htmlTable | https://www.bco-dmo.org/dataset/933334
| https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_933334_v1.rss | https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_933334_v1&showErrors=false&email= | BCO-DMO | bcodmo_dataset_933334_v1 |