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https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_963393_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_963393_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_963393_v1/ public [Combined Selfish Bacteria Cell Counts 2023-2024] - Cell counts exhibiting 'Selfish' uptake in the Western North Atlantic, in Danish Coastal Seawater, and Abyssopelagic Waters off the Eastern Coast of Japan under varying hydrostatic pressures, 2023-2024 (Collaborative Research: Pressure effects on microbially-catalyzed organic matter degradation in the deep ocean) Heterotrophic bacteria and archaea (here: microbes) are critical drivers of the ocean's biogeochemical cycles, active throughout the depth of the ocean. Their capabilities and limitations help determine the rates and locations at which carbon and nutrients are regenerated, as well as the extent to which organic matter is preserved (Hedges 1992). In the deep ocean, at bathy- and abyssopelagic depths (ca. 1000-6000m), these communities are dependent upon the sinking flux of particulate organic matter (POM) from the surface ocean (Bergauer et al. 2018). This dependence means that heterotrophic microbial communities must produce the extracellular enzymes required to solubilize and hydrolyze high molecular weight (HMW) POM to sizes substrates suitable for cellular uptake. A recent global-scale investigation of deep-sea microbes in fact found that the genetic potential for exported (extracellular) enzymes among bacteria in deep waters was far greater than for communities in surface or mesopelagic waters (Zhao et al. 2020). We have new evidence that a substantial fraction of bacteria in bottom water from the North Atlantic Ocean use a specialized set of extracellular enzymes to rapidly take up HMW polysaccharides (Giljan et al. 2022), a substrate processing mechanism that would not be detected with the low molecular weight substrates used in most prior studies of microbial activity in the deep ocean (Nagata et al. 2010).\n \nThrough our collaboration with the Danish Center for Hadal Research, we were able to use pressurization systems and in situ specialized equipment to investigate the effects of pressures characteristic of bathy- and abyssopelagic depths on microbial communities and their extracellular enzymes in the open North Atlantic Ocean, in Danish Coastal Seawater, and abyssopelagic waters off the Eastern Coast of Japan. \n \nHere we present, in collaboration with colleagues from the Max Planck Institute for Marine Microbiology, the detection and quantification of microbial cells exhibiting selfish uptake behavior of fluorescently-labeled HMW polysaccharides. This dataset includes sample collection metadata, environmental variables, experimental variables, the number of cells detected exhibiting 'selfish' uptake, and total cellular abundance.\n\ncdm_data_type = Other\nVARIABLES:\ndeployment (unitless)\n... (22 more variables)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_963393_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_963393_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_963393_v1/index.htmlTable https://osprey.bco-dmo.org/dataset/963393 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_963393_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_963393_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_963393_v1

 
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