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| griddap | Subset | tabledap | Make A Graph | wms | files | Accessible | Title | Summary | FGDC | ISO 19115 | Info | Background Info | RSS | Institution | Dataset ID | |
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| log in | [Acropora cervicornis protein measurements: nutrient- and disease-exposed] - Acropora cervicornis protein measurements: nutrient- and disease-exposed from samples collected at Mote Marine Laboratory in situ nursery from June to July 2022 (Collaborative Research: Tracking the interacting roles of the environment, host genotype, and a novel Rickettsiales in coral disease susceptibility) | In the summer of 2022, 20 ramets each of the Acropora cervicornis genotypes ML-AC-36 and ML-AC-46 were collected from Mote's in situ coral nursery and subjected to three weeks of elevated nitrate, ammonium, and phosphate in the form of a slow-release fertilizer. The duration and concentration of this exposure were selected based on prior experiments conducted at Mote Marine Laboratory, which were found to alter microbial community profiles and growth rates in A. cervicornis (Klinges et al., 2022, 2023). During experiment, corals were held in 2-gallon aquaria with 5 corals per aquarium. As Mote Marine Laboratory's experimental aquarium system is plumbed into nearshore coastal water and thus has a higher nutrient load than the reef, an additional subset of 18 ramets of each genotype was collected from Mote's in situ coral nursery immediately prior to disease challenge to evaluate the impact of nearshore water on disease response. All ramets in the disease group were subjected to disease challenge in the form of a tissue homogenate produced from diseased fragments of random genotypes of A. cervicornis. Ramets in a comparative unexposed group were exposed to a homogenate produced from healthy fragments of random genotypes of A. cervicornis. To evaluate the effect of Aquarickettsia infection; nutrient enrichment; disease exposure; and the combination of these factors on coral immune function, a number of immune related proteins and antioxidants were measured using microplate assays from a total of 55 samples taken prior to disease exposure and following disease exposure as corals either resisted or developed disease. Total host protein and the antioxidant superoxide dismutase were found to be higher in healthy corals regardless of genotype or nutrient enrichment. Phenoloxidase and prophenoloxidase were higher in concentration in diseased samples compared to healthy samples. Disease-exposed but apparently healthy corals had higher superoxide dismutase, prophenoloxidase, and peroxidase activity than either healthy unexposed corals or diseased corals despite total protein concentrations that were lower than healthy unexposed corals.\n\ncdm_data_type = Other\nVARIABLES:\nFull_ID (unitless)\nDate (unitless)\nTP (mg)\nPOX (ΔAbs470nm)\nNormalized_POX (ΔAbs470nm/mg protein)\n... (17 more variables)\n | BCO-DMO | bcodmo_dataset_924466_v1 | ||||||||||||
| log in | [Lab experimental time series of T. suecica densities under fluctuating temperatures] - Time series of T. suecica densities under fluctuating temperatures experiment from May 2023 to Aug 2023 (A novel time-structured framework to account for the cryptic effects of temperature fluctuations on population dynamics) | This dataset includes the densities (count/mL) of the alga Tetraselmis suecica (LB 2286) cultures grown from a UTEX sample. The counts were obtained using a Beckman Coulter Z2 Particle Counter in the lab over a five-day period under different experimental temperature regimes. The experiment took place between 2023-05-23 and 2023-08-18. The goal of the experiment was to determine how mean temperature and temperature fluctuation frequency affected the growth rate of T. suecica. By comparing the growth rate of T. suecica under constant vs. variable temperatures, one can determine the historical or legacy effects of past temperature variation on subsequent organismal performance. These experiments were conducted by members of the Brian Helmuth Lab at Northeastern University.\n\ncdm_data_type = Other\nVARIABLES:\nfull_ID (unitless)\ntime (Measurement_datetime, seconds since 1970-01-01T00:00:00Z)\ndays_since_start (days)\ntemperature (degrees Celsius)\ntreatment (unitless)\nreplicate (unitless)\ncount_replicate (unitless)\nfinal_count (cells/mL)\n | BCO-DMO | bcodmo_dataset_953492_v1 |