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| griddap | Subset | tabledap | Make A Graph | wms | files | Accessible | Title | Summary | FGDC | ISO 19115 | Info | Background Info | RSS | Institution | Dataset ID | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| log in | [PcaFV Prevalence] - Prevalence of PcaFV in Apostichopus californicus specimens collected in Southeast Alaska from Jun to Nov 2021 (Exploring the role of boundary layer microbial remineralization in flavivirus-host dynamics) | Understanding the prevalence and load of aquatic invertebrate flaviviruses is essential to estimating risk to fisheries. We surveyed the prevalence of the Apostichopus californicus (urn:lsid:marinespecies.org:taxname:529363) associated flavivirus (PcaFV) using two approaches: Loop-mediated isothermal amplification (LAMP) and quantitative reverse transcriptase PCR. We targeted this survey around two populations of A. californicus: specimens collected from Sitka Sound, Sitka, Alaska (Baranhof Island) and at undisclosed locations in the Ketchikan sea cucumber commercial fishery. We found that LAMP detected PcaFV in 21 % of tested specimens, and qRT-PCR in 88% of tested specimens. The mean load of PcaFV in copies per ng RNA extracted was significantly higher in specimens that were positive by LAMP compared to those in which LAMP did not detect PcaFV. This dataset contains PcaFV prevalence determined by RT-LAMP and qRT-PCR, as well as associated collection metadata.\n\ncdm_data_type = Other\nVARIABLES:\nSample_Name (unitless)\nSample_Identity (unitless)\nGeographic_Location (unitless)\nTissue_Type (unitless)\nPcaFV_Quantity_qRTPCR (copies ng⁻¹ RNA)\nLAMP_Detection (unitless)\nDate_of_Collection (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\n | BCO-DMO | bcodmo_dataset_984849_v1 | ||||||||||||
| https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_775451.subset | https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_775451 | https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_775451.graph | https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_775451/ | public | [Sponge holobiont accessions and metadata] - NCBI accessions and metadata associated with Caribbean sponge metagenomes collected from Curacao, Belize, Cayman Islands and St. Croix, 2009 and 2017-2018 (Collaborative Research: Dimensions: Evolutionary Ecology of Sponges and Their Microbiome Drives Sponge Diversity on Coral Reefs) | NCBI accessions and metadata associated with Caribbean sponge metagenomes collected from Curacao, Belize, Cayman Islands and St. Croix, 2009 and 2017-2018.\n\ncdm_data_type = Other\nVARIABLES:\nBioProject (Bio Project, unitless)\nBioSample (Bio Sample, unitless)\nSample_name (unitless)\nsample_type (unitless)\nhost (unitless)\ncollection_date (unitless)\ngeographic_location (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\nsource_material_identifiers (unitless)\ndepth_ (Depth, feet seawater (fsw) or meters)\n | https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_775451_fgdc.xml | https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_775451/index.htmlTable | https://www.bco-dmo.org/dataset/775451
| https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_775451.rss | https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_775451&showErrors=false&email= | BCO-DMO | bcodmo_dataset_775451 |