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https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_964174_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_964174_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_964174_v1/ public [Targeted metabolomics data from coral and sea anemone larvae exposed to normoxic or hypoxic conditions] - Targeted metabolomics data from coral and sea anemone larvae exposed to normoxic or hypoxic conditions in lab and field experiments performed in USA and Bermuda during 2023-2024 (CAREER: Helping or hindering? Determining the influence of repetitive marine heatwaves on acclimatization of reef-building corals across biological scales) These data arose from larvae of the sea anemone Nematostella vectensis and corals Galaxea fascicularis and Porites astreoides exposed to normoxic (i.e., control) or hypoxic conditions. Larvae were sampled in groups of 20-30 and these samples were processed for targeted metabolomics at the University of Pennsylvania Metabolomics Core. The dataset contains the species from which metabolomics data originate, experimental treatment (normoxia or hypoxia), experiment metadata, and abundance of each metabolite in each sample.\n\ncdm_data_type = Other\nVARIABLES:\nSpecies (units)\nTreatment (units)\nCohort (units)\nGroup (units)\nLife_stage (units)\nHours_post_treatment (hours)\nADP (nmol mg protein-1)\nAMP (nmol mg protein-1)\nATP (nmol mg protein-1)\ncAMP (nmol mg protein-1)\nCMP (nmol mg protein-1)\nCTP (nmol mg protein-1)\nGDP (nmol mg protein-1)\nGMP (nmol mg protein-1)\nGTP (nmol mg protein-1)\nITP (nmol mg protein-1)\nNAD (nmol mg protein-1)\nNADP (nmol mg protein-1)\nNAM (nmol mg protein-1)\nNMN (nmol mg protein-1)\nTTP (nmol mg protein-1)\nUMP (nmol mg protein-1)\n... (67 more variables)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_964174_v1/index.htmlTable https://osprey.bco-dmo.org/dataset/964174 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_964174_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_964174_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_964174_v1
log in [Thalassiosira pseudonana CCMP1335 endometabolite uptake by Ruegeria pomeroyi DSS-3] - Thalassiosira pseudonana CCMP1335 endometabolite uptake by Ruegeria pomeroyi DSS-3 (Effects of Climate Change Variables on Microbial Autotroph-Heterotroph Carbon Flux) The ocean's temperature increase has fundamental implications for physiological rates and processes of marine microbes. In this study, a marine diatom Thalassiosira pseudonana CCMP1335 was acclimated for three months at temperatures below (14°C), equal to (20°C), and above (28°C). Heterotrophic bacterium Ruegeria pomeroyi DSS-3 was inoculated into cultures, and transporter expression was compared between temperatures.  R. pomeroyi transporter expression leveraged as  a biosensor of available diatom exometabolites indicated temperature-related substitution of diatom osmolytes dimethylsulfoniopropionate (DMSP), dihydroxypropanesulfonate (DHPS), and homarine (dominating carbon transfer at lower temperatures) with glycine betaine and choline (dominating at higher temperatures). T. pseudonana endometabolome pools and biosynthetic pathway expression indicated increased availability of amino acids and glycerol-3-phosphate at higher temperatures. Overall trends across datasets supported a greater importance of organic sulfur compounds in diatom-bacterial metabolite transfer at lower temperatures and greater importance of organic nitrogen compounds at higher temperatures.\n\ncdm_data_type = Other\nVARIABLES:\nExperiment (unitless)\nSample_ID (unitless)\nMetabolomics_Workbench_Project_ID (unitless)\nMetabolomics_Workbench_Study_ID (unitless)\nMetabolomics_Workbench_Sample_ID (unitless)\nNumber (unitless)\nTemperature (degrees Celsius (°C))\nReplicate (unitless)\nCulture (unitless)\nJGI_Sample_ID (unitless)\nTime_Point_h (hours (h))\nBacteria_presence (unitless)\nGlycine_Betaine (NMR peak intensity per biovolume)\nProline (NMR peak intensity per biovolume)\nDHPS (NMR peak intensity per biovolume)\n... (13 more variables)\n BCO-DMO bcodmo_dataset_928203_v1

 
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