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https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_511526 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_511526.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_511526/ public [growth phase exopolymers] - Experimental results: Exopolymer and carbohydrate production by diatoms with growth; conducted at the Thornton lab, TAMU from 2007-2012 (Diatom EPS Production project) (Effect of Temperature on Extracellular Polymeric Substance Production (EPS) by Diatoms) Data from laboratory experiment on exopolymer and carbohydrate production by\nthe diatoms Thalassiosira weissflogii (CCMP 1051), Skeletonema marinoi (CCMP\n1332), and Cylindrotheca closterium (CCMP 339) during the growth to death\nphases of the cultures.\n \nRelated references:  \n Chen, J. 2014. Factors affecting carbohydrate production and the formation\nof transparent exopolymer particles (TEP) by diatoms. Ph.D. dissertation,\nTexas A&M University, College Station, TX.\n\ncdm_data_type = Other\nVARIABLES:\nspecies (dimensionless)\ngrowth_phase (dimensionless)\nday (dimensionless)\nculture (dimensionless)\ncell_abundance (cells per milliliter (mL-1))\ncell_vol_mean (cubic micrometers (um^3))\nchla (Concentration Of Chlorophyll In Sea Water, micrograms per liter (ug L-1))\nchla_per_cell (Concentration Of Chlorophyll In Sea Water, picograms per cell (pg cell-1))\nchla_per_cell_vol (Concentration Of Chlorophyll In Sea Water, femtograms per cubic micrometer (fg um-3))\ntot_carb (micrograms per milliliter (ug mL-1))\ntot_carb_per_cell (picograms per cell (pg cell-1))\ntot_carb_per_cell_vol (femtograms per cubic micrometer (fg um-3))\ncolloidal_carb (micrograms per milliliter (ug mL-1))\ncollodial_per_cell (picograms per cell (pg cell-1))\ncolloidal_per_cell_vol (femtograms per cubic micrometer (fg um-3))\nEPS_carb (micrograms per milliliter (ug mL-1))\nEPS_carb_per_cell (picograms per cell (pg cell-1))\nEPS_carb_per_cell_vol (femtograms per cubic micrometer (fg um-3))\nHW_carb (micrograms per milliliter (ug mL-1))\n... (21 more variables)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_511526/index.htmlTable https://www.bco-dmo.org/dataset/511526 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_511526.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_511526&showErrors=false&email= BCO-DMO bcodmo_dataset_511526
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_897359_v1 https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_897359_v1/ public [Ruegeria pomeroyi DOP hydrolysis rates] - Dissolved organic phosphorus (DOP) hydrolysis rates from Ruegeria pomeroyi laboratory cultures (Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle) Dissolved organic phosphorus (DOP) hydrolysis rates from marine bacterium Ruegeria pomeroyi laboratory cultures.\n\nThese data were collected as part of a study of \"Dissolved organic phosphorus utilization by the marine bacterium Ruegeria pomeroyi DSS-3 reveals chain length-dependent polyphosphate degradation\" (Adams et al., 2022).\n\nStudy abstract:\nDissolved organic phosphorus (DOP) is a critical nutritional resource for marine microbial communities. However, the relative bioavailability of different types of DOP, such as phosphomonoesters (P-O-C) and phosphoanhydrides (P-O-P), is poorly understood. Here we assess the utilization of these P sources by a representative bacterial copiotroph, Ruegeria pomeroyi DSS-3. All DOP sources supported equivalent growth by R. pomeroyi, and all DOP hydrolysis rates were upregulated under phosphorus depletion (-P). A long-chain polyphosphate (45polyP) showed the lowest hydrolysis rate of all DOP substrates tested, including tripolyphosphate (3polyP). Yet the upregulation of 45polyP hydrolysis under -P was greater than any other substrate analyzed. Proteomics revealed three common P acquisition enzymes potentially involved in polyphosphate utilization, including two alkaline phosphatases, PhoD and PhoX, and one 5'-nucleotidase (5'-NT). Results from DOP substrate competition experiments show that these enzymes likely have broad substrate specificities, including chain length-dependent reactivity toward polyphosphate. These results confirm that DOP, including polyP, are bioavailable nutritional P sources for R. pomeroyi, and possibly other marine heterotrophic bacteria. Furthermore, the chain-length dependent mechanisms, rates and regulation of polyP hydrolysis suggest that these processes may influence the composition of DOP and the overall recycling of nutrients within marine dissolved organic matter.\n\ncdm_data_type = Other\nVARIABLES:\nMedia_Type (unitless)\nGrowth_Phase (unitless)\nSample_Type (unitless)\nDOP_Substrate (unitless)\nHydrolysis_Rate (umol Pi L-1 hr-1)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_897359_v1/index.htmlTable https://www.bco-dmo.org/dataset/897359 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_897359_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_897359_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_897359_v1

 
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