BCO-DMO ERDDAP
Accessing BCO-DMO data 		log in    
Brought to you by BCO-DMO    
 
 
griddap Subset tabledap Make A Graph wms files Accessible Title Summary FGDC ISO 19115 Info Background Info RSS Email Institution Dataset ID
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_963407_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_963407_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_963407_v1/ public [AE2413 Bacterial productivity] - Bacterial productivity of samples from three stations in the Western North Atlantic aboard R/V Atlantic Explorer cruise AE2413, during May 2024 (Collaborative Research: Pressure effects on microbially-catalyzed organic matter degradation in the deep ocean) Heterotrophic bacteria and archaea (here: microbes) are critical drivers of the ocean's biogeochemical cycles, active throughout the depth of the ocean. Their capabilities and limitations help determine the rates and locations at which carbon and nutrients are regenerated, as well as the extent to which organic matter is preserved (Hedges 1992). In the deep ocean, at bathy- and abyssopelagic depths (ca. 1000-6000m), these communities are dependent upon the sinking flux of particulate organic matter (POM) from the surface ocean (Bergauer et al. 2018). This dependence means that heterotrophic microbial communities must produce the extracellular enzymes required to solubilize and hydrolyze high molecular weight (HMW) POM to sizes substrates suitable for cellular uptake. A recent global-scale investigation of deep-sea microbes in fact found that the genetic potential for exported (extracellular) enzymes among bacteria in deep waters was far greater than for communities in surface or mesopelagic waters (Zhao et al. 2020). We have new evidence that a substantial fraction of bacteria in bottom water from the North Atlantic Ocean use a specialized set of extracellular enzymes to rapidly take up HMW polysaccharides (Giljan et al. 2021), a substrate processing mechanism that would not be detected with the low molecular weight substrates used in most prior studies of microbial activity in the deep ocean (Nagata et al. 2010).\n \nThrough our collaboration with the Danish Center for Hadal Research, we were able to use pressurization systems and in situ specialized equipment to investigate the effects of pressures characteristic of bathy- and abyssopelagic depths on microbial communities and their extracellular enzymes in the open North Atlantic Ocean.   \n \nHere we present the measurement of 3H-leucine incorporation by heterotrophic bacteria using a cold trichloroacetic acid (TCA) and microcentrifuge extraction method (Kirchman, 2001) at different sites in the Western North Atlantic aboard R/V Atlantic Explorer during during the research cruise AE2413 (2024-05-09 to 2024-05-28).  All work and incubations were performed in a UNOLS isotope lab, or within designated areas at the University of North Carolina at Chapel HIll post cruise. This dataset contains collection metadata, environmental conditions, sample types and treatments, incubation conditions, substrate types, radioactivity measurements, and calculated incorporation rates of 3H-leucine.\n\ncdm_data_type = Other\nVARIABLES:\ndeployment (unitless)\n... (24 more variables)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_963407_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_963407_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_963407_v1/index.htmlTable https://osprey.bco-dmo.org/dataset/963407 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_963407_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_963407_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_963407_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_968956_v1/ public [AE2413 FlaPS bulk] - Polysaccharide hydrolase activities from water samples collected at various sites under varying hydrostatic pressures in the Western North Atlantic aboard R/V Atlantic Explorer cruise AE2413 in May 2024 (Collaborative Research: Pressure effects on microbially-catalyzed organic matter degradation in the deep ocean) Heterotrophic bacteria and archaea (here: microbes) are critical drivers of the ocean's biogeochemical cycles, active throughout the depth of the ocean. Their capabilities and limitations help determine the rates and locations at which carbon and nutrients are regenerated, as well as the extent to which organic matter is preserved (Hedges 1992). In the deep ocean, at bathy- and abyssopelagic depths (ca. 1000-6000m), these communities are dependent upon the sinking flux of particulate organic matter (POM) from the surface ocean (Bergauer et al. 2018). This dependence means that heterotrophic microbial communities must produce the extracellular enzymes required to solubilize and hydrolyze high molecular weight (HMW) POM to sizes substrates suitable for cellular uptake. A recent global-scale investigation of deep-sea microbes in fact found that the genetic potential for exported (extracellular) enzymes among bacteria in deep waters was far greater than for communities in surface or mesopelagic waters (Zhao et al. 2020). We have new evidence that a substantial fraction of bacteria in bottom water from the North Atlantic Ocean use a specialized set of extracellular enzymes to rapidly take up HMW polysaccharides (Giljan et al. 2021), a substrate processing mechanism that would not be detected with the low molecular weight substrates used in most prior studies of microbial activity in the deep ocean (Nagata et al. 2010).\n \nThrough our collaboration with the Danish Center for Hadal Research, we were able to use pressurization systems and in situ specialized equipment to investigate the effects of pressures characteristic of bathy- and abyssopelagic depths on microbial communities and their extracellular enzymes in the open North Atlantic Ocean.  \n \nHere we present the measurement of polysaccharide hydrolase activities above that measured for killed controls from various sites under varying hydrostatic pressures in the Western North Atlantic aboard R/V Atlantic Explorer, cruise AE2413 in May 2024.\n\ncdm_data_type = Other\nVARIABLES:\ndeployment (unitless)\nstation (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\n... (26 more variables)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_968956_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_968956_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_968956_v1/index.htmlTable https://osprey.bco-dmo.org/dataset/968956 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_968956_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_968956_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_968956_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_963393_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_963393_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_963393_v1/ public [Combined Selfish Bacteria Cell Counts 2023-2024] - Cell counts exhibiting 'Selfish' uptake in the Western North Atlantic, in Danish Coastal Seawater, and Abyssopelagic Waters off the Eastern Coast of Japan under varying hydrostatic pressures, 2023-2024 (Collaborative Research: Pressure effects on microbially-catalyzed organic matter degradation in the deep ocean) Heterotrophic bacteria and archaea (here: microbes) are critical drivers of the ocean's biogeochemical cycles, active throughout the depth of the ocean. Their capabilities and limitations help determine the rates and locations at which carbon and nutrients are regenerated, as well as the extent to which organic matter is preserved (Hedges 1992). In the deep ocean, at bathy- and abyssopelagic depths (ca. 1000-6000m), these communities are dependent upon the sinking flux of particulate organic matter (POM) from the surface ocean (Bergauer et al. 2018). This dependence means that heterotrophic microbial communities must produce the extracellular enzymes required to solubilize and hydrolyze high molecular weight (HMW) POM to sizes substrates suitable for cellular uptake. A recent global-scale investigation of deep-sea microbes in fact found that the genetic potential for exported (extracellular) enzymes among bacteria in deep waters was far greater than for communities in surface or mesopelagic waters (Zhao et al. 2020). We have new evidence that a substantial fraction of bacteria in bottom water from the North Atlantic Ocean use a specialized set of extracellular enzymes to rapidly take up HMW polysaccharides (Giljan et al. 2022), a substrate processing mechanism that would not be detected with the low molecular weight substrates used in most prior studies of microbial activity in the deep ocean (Nagata et al. 2010).\n \nThrough our collaboration with the Danish Center for Hadal Research, we were able to use pressurization systems and in situ specialized equipment to investigate the effects of pressures characteristic of bathy- and abyssopelagic depths on microbial communities and their extracellular enzymes in the open North Atlantic Ocean, in Danish Coastal Seawater, and abyssopelagic waters off the Eastern Coast of Japan. \n \nHere we present, in collaboration with colleagues from the Max Planck Institute for Marine Microbiology, the detection and quantification of microbial cells exhibiting selfish uptake behavior of fluorescently-labeled HMW polysaccharides. This dataset includes sample collection metadata, environmental variables, experimental variables, the number of cells detected exhibiting 'selfish' uptake, and total cellular abundance.\n\ncdm_data_type = Other\nVARIABLES:\ndeployment (unitless)\n... (22 more variables)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_963393_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_963393_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_963393_v1/index.htmlTable https://osprey.bco-dmo.org/dataset/963393 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_963393_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_963393_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_963393_v1
log in [Peptidase and glucosidase activities from mesocosm and bulk water incubations.] - Peptidase and glucosidase activities from mesocosm and bulk water incubations from waters taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 in May and June, 2022. (Substrate structural complexity and abundance control distinct mechanisms of microbially-driven carbon cycling in the ocean) This dataset includes the measurements of leucine aminopeptidase, glucosidase, and endo-acting (mid-chain cleaving) peptidase activities from bulk and mesocosm incubations from waters taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 (2022-05-24 to 2022-06-12). Waters for bulk incubation were taken at three sites and various depths, mesocosm incubations were taken at two sites and two depths. \n                 \nThis research tested the hypothesis that the mechanism of polysaccharide processing is related to the cost to a cell of producing the enzymes required for its hydrolysis, and the probability that a cell will receive sufficient return on investment for producing the enzymes. Our conceptual model suggests that external (extracellular) hydrolysis is favored when organic matter is abundant, or when enzyme production costs can be shared (e.g., on particles, in biofilms); selfish uptake (hydrolysis without production of low molecular weight products in the environment) would be a better strategy when high molecular weight (HMW) organic matter is scarce, and particularly when the HMW organic matter is very complex. \n\nSeawater was sampled from four depths at our initial station, and the deep chlorophyll maximum and bottom water from two subsequent stations differing in typical extent of primary productivity. \n\nWe incubated mesocosms of seawater from the deep chlorophyll maximum and from bottom water from two stations differing in typical extent of primary productivity. We changed organic matter availability by adding to three mesocosms high molecular weight organic matter (dissolved and particulate) from diatoms, three mesocosms received an addition of the polysaccharide fucoidan, and three mesocosms were amended with the polysaccharide arabinogalactan. One mesocosm from each depth and station was left unamended. \n\nThese data help test this model because they provide information about the activities of exo-acting glucosidases and an exo-acting peptidase, plus endo-acting peptidases, all of which are used to hydrolyze high molecular weight organic matter. Activities of these enzymes differed by station as well as by depth\n\ncdm_data_type = Other\nVARIABLES:\nIncubation (unitless)\n... (28 more variables)\n BCO-DMO bcodmo_dataset_956085_v1

 
ERDDAP, Version 2.22
Disclaimers | Privacy Policy | Contact