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log in [AE2413 Bacterial productivity] - Bacterial productivity of samples from three stations in the Western North Atlantic aboard R/V Atlantic Explorer cruise AE2413, during May 2024 (Collaborative Research: Pressure effects on microbially-catalyzed organic matter degradation in the deep ocean) Heterotrophic bacteria and archaea (here: microbes) are critical drivers of the ocean's biogeochemical cycles, active throughout the depth of the ocean. Their capabilities and limitations help determine the rates and locations at which carbon and nutrients are regenerated, as well as the extent to which organic matter is preserved (Hedges 1992). In the deep ocean, at bathy- and abyssopelagic depths (ca. 1000-6000m), these communities are dependent upon the sinking flux of particulate organic matter (POM) from the surface ocean (Bergauer et al. 2018). This dependence means that heterotrophic microbial communities must produce the extracellular enzymes required to solubilize and hydrolyze high molecular weight (HMW) POM to sizes substrates suitable for cellular uptake. A recent global-scale investigation of deep-sea microbes in fact found that the genetic potential for exported (extracellular) enzymes among bacteria in deep waters was far greater than for communities in surface or mesopelagic waters (Zhao et al. 2020). We have new evidence that a substantial fraction of bacteria in bottom water from the North Atlantic Ocean use a specialized set of extracellular enzymes to rapidly take up HMW polysaccharides (Giljan et al. 2021), a substrate processing mechanism that would not be detected with the low molecular weight substrates used in most prior studies of microbial activity in the deep ocean (Nagata et al. 2010).\n \nThrough our collaboration with the Danish Center for Hadal Research, we were able to use pressurization systems and in situ specialized equipment to investigate the effects of pressures characteristic of bathy- and abyssopelagic depths on microbial communities and their extracellular enzymes in the open North Atlantic Ocean.   \n \nHere we present the measurement of 3H-leucine incorporation by heterotrophic bacteria using a cold trichloroacetic acid (TCA) and microcentrifuge extraction method (Kirchman, 2001) at different sites in the Western North Atlantic aboard R/V Atlantic Explorer during during the research cruise AE2413 (2024-05-09 to 2024-05-28).  All work and incubations were performed in a UNOLS isotope lab, or within designated areas at the University of North Carolina at Chapel HIll post cruise. This dataset contains collection metadata, environmental conditions, sample types and treatments, incubation conditions, substrate types, radioactivity measurements, and calculated incorporation rates of 3H-leucine.\n\ncdm_data_type = Other\nVARIABLES:\ndeployment (unitless)\n... (24 more variables)\n BCO-DMO bcodmo_dataset_963407_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_948396_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_948396_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_948396_v1/ public [CliOMZ Dark DIC fixation rates] - Dark DIC Fixation Rates collected from CliOMZ AT50-10 in the Eastern Pacific Ocean from May to June 2023 (CliOMZ project) (Collaborative Research: Underexplored Connections between Nitrogen and Trace Metal Cycling in Oxygen Minimum Zones Mediated by Metalloenzyme Inventories) These data include dark dissolved inorganic carbon (DIC) fixation rates measured on R/V Atlantis (CliOMZ AT50-10 expedition) from Golfito, Costa Rica to San Diego, USA in May-June 2023.  We aimed at quantifying dark DIC fixation rates associated with nitrification by specifically inhibiting ammonia-oxidizing microorganisms. Instruments used were a CTD profiler and a scintillation counter (Perkin-Elmer Tri-Carb 2910 TR).\n\ncdm_data_type = Other\nVARIABLES:\nCruise (unitless)\nDate (unitless)\nlongitude (Latitude, degrees_east)\nlatitude (Longitude, degrees_north)\nStation (unitless)\ndepth (m)\nTreatment (unitless)\nDIC_fixation (nanomoles per liter per day (nmol/L/d))\nIncubation_time (hours (h))\nDIC_conc (micromoles per liter (umol/L))\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_948396_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_948396_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_948396_v1/index.htmlTable https://www.bco-dmo.org/dataset/948396 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_948396_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_948396_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_948396_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_948411_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_948411_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_948411_v1/ public [CliOMZ Heterotrophic production rates] - Heterotrophic Production Rates collected from CliOMZ AT50-10 in the Eastern Pacific Ocean from May to June 2023 (CliOMZ project) (Collaborative Research: Underexplored Connections between Nitrogen and Trace Metal Cycling in Oxygen Minimum Zones Mediated by Metalloenzyme Inventories) These data include heterotrophic production rates measured on R/V Atlantis (CliOMZ AT50-10 expedition) from Golfito, Costa Rica to San Diego, USA in May-June 2023.  We aimed at comparing heterotrophic and chemoautotrophic microbial activities at the selected study sites. Instruments used were a CTD profiler and a scintillation counter (Perkin-Elmer Tri-Carb 2910 TR).\n\ncdm_data_type = Other\nVARIABLES:\nCruise (unitless)\nDate (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\nStation (unitless)\nCast (unitless)\ndepth (m)\nHet_production (nanomoles carbon per liter per day (nmol C/L/d))\nTreatment (unitless)\nIncubation_time (hours (h))\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_948411_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_948411_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_948411_v1/index.htmlTable https://www.bco-dmo.org/dataset/948411 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_948411_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_948411_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_948411_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489471.subset https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489471 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489471.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_489471/ public [pteropod shell dissolution] - Pteropod shell dissolution in natural and high-CO2 environments from samples collected on RRS James Clark Ross cruise JR177 in the Scotia Sea, Southern Ocean from 2007-2008 (An Investigation of the Role of Nutrition in the Coral Calcification Response to Ocean Acidification) This dataset contains data from a study of pteropod shell dissolution on individuals exposed to CO2-enriched seawater. The data include the amount of dissolution as well as the physical and chemical parameters on which carbonate chemistry parameters were calculated.\n\ncdm_data_type = Other\nVARIABLES:\ntreatment (dimensionless)\nincubation_time (days)\nN (dimensionless)\nspecies (dimensionless)\nsal (dimensionless)\ntemp (Temperature, degrees Celsius)\nphosphate (Mass Concentration Of Phosphate In Sea Water, micromoles per kilogram (umol/kg))\nsilicate (Mass Concentration Of Silicate In Sea Water, micromoles per kilogram (umol/kg))\nalk_tot (micromoles per kilogram (umol/kg))\nalk_tot_stdev (micromoles per kilogram (umol/kg))\nDIC (micromoles per kilogram (umol/kg))\nDIC_stdev (micromoles per kilogram (umol/kg))\npH (Sea Water Ph Reported On Total Scale, dimensionless)\npH_stdev (P H Stdev, dimensionless)\npCO2 (P CO2, microatmospheres (uatm))\npCO2_stdev (P CO2 Stdev, microatmospheres (uatm))\nbicarbonate (micromoles per kilogram (umol/kg))\nbicarbonate_stdev (micromoles per kilogram (umol/kg))\ncarbonate (micromoles per kilogram (umol/kg))\ncarbonate_stdev (micromoles per kilogram (umol/kg))\nomega_Arg (dimensionless)\nomega_Arg_stdev (dimensionless)\nnon_diss (percentage (%))\nnon_diss_stdev (percentage (%))\ndiss_rate1 (percentage (%))\n... (5 more variables)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_489471/index.htmlTable https://www.bco-dmo.org/dataset/489471 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_489471.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_489471&showErrors=false&email= BCO-DMO bcodmo_dataset_489471

 
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