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https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_921924_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_921924_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_921924_v1/ public [DDA nutrient acquisition] - Hemiaulus-Richelia physiological response to different nitrogen sources (META-DDA: METabolic Activities of Diatom-Diazotroph Associations) Diatom-diazotroph associations (DDAs) play an important role at the base of the food web by fixing both carbon dioxide and nitrogen gas into organic matter. The ability of DDAs to fix nitrogen allows the relatively large diatom host to survive under nitrogen-deplete conditions and, thus, contribute significantly to organic carbon export in oligotrophic waters. Yet, relatively little is known about this symbiosis, in part because it has historically been difficult to maintain DDAs in culture. Here, we isolated the DDA Hemiaulus-Richelia from the Sargasso Sea and developed a protocol to maintain it in laboratory culture for long time periods (years). Experiments were conducted to determine how Hemiaulus-Richelia responds to different nitrogen sources with measurements of growth and nitrogen fixation.\n\ncdm_data_type = Other\nVARIABLES:\nFlask (unitless)\nTime_gp (unitless)\nTimepoint (days)\nTreatment (unitless)\nRFU_Turner (relative fluorescence units)\nchla (micrograms per cell (ug/cell))\nphaeophytin (micrograms per cell (ug/cell))\nHemiaulus_cellspermL (cells per milliliter (cells/mL))\nRichelia_cellspermL (cells per milliliter (cells/mL))\nAsymb_cellspermL (cells per milliliter (cells/mL))\nHemaiulus_mean_SA (square micrometers (um^2))\nHemaiulus_mean_vol (cubic micrometers (um^3))\nRichelia_mean_SA (square micrometers (um^2))\nRichelia_mean_vol (cubic micrometers (um^3))\nNO3_uM (micromolar (uM))\nNO2_uM (micromolar (uM))\nNH4_uM (micromolar (uM))\nSiOH4_uM (micromolar (uM))\nPO4_uM (micromolar (uM))\n... (15 more variables)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_921924_v1/index.htmlTable https://osprey.bco-dmo.org/dataset/921924 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_921924_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_921924_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_921924_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_917767_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_917767_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_917767_v1/ public [Subterranean Estuary In Situ Tracer Experiment Incubation Data, 2019] - In situ tracer injection experiment conducted with 15N-labeled ammonium in a shallow, sandy subterranean estuary in Gloucester Point, USA  in August 2019. (Collaborative Research:  Cryptic nitrogen cycling in the anoxic subterranean estuary) These data are the result of an in situ tracer injection experiment conducted with 15N-labeled ammonium to determine the fate and transport rates of ammonium within a shallow, sandy subterranean estuary in Gloucester Point, VA, USA. \n\nReplicate injections of 15N-labeled ammonium, sulfur hexaflouride, and bromide amended porewater were injected into piezometers at 50cm.  Porewater was then collected overtime from the injection piezometers and tracer piezometers surrounding the injection site ranging in depth from 40-60cm.  At each time point, samples were collected to analyze dissolved inorganic nitrogen (nitrate, nitrite, and ammonium) concentrations, sulfur hexaflouride, bromide, chloride. Nitrate and nitrite samples from porewater were analyzed with an isotope ratio mass spectrometer in order to assess the 15N enrichment of the nitrate in each sample resulting in a delta value (d15N) that allows for the calculation of the mole fraction of 15N-labeled nitrite and nitrate in porewater at each time point. The production or consumption over time constitutes subterranean estuary nitrogen cycling rates (e.g. nitrification, denitrification, etc.).\n\ncdm_data_type = Other\nVARIABLES:\nSite_Name (unitless)\nLatitude (degrees_north)\nlongitude (degrees_east)\nPiezometer (unitless)\nSample_ID (unitless)\nTime_Point (unitless)\nDate (unitless)\nTime (unitless)\nHours_after_Injection (hours (hrs))\nBromide_uM (micromoles per liter (uM))\nChloride_mM (millimoles per liter (mM)\nSF6_ppbv (parts per billion per volume (ppbv))\nSF6_pM (picoMolar (pM))\nNox_uM (micromoles per liter (uM))\n... (4 more variables)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_917767_v1/index.htmlTable https://www.bco-dmo.org/dataset/917767 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_917767_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_917767_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_917767_v1

 
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