Accessing BCO-DMO data
Brought to you by BCO-DMO
|
|
BCO-DMO ERDDAP
Accessing BCO-DMO data |
log in
Brought to you by BCO-DMO |
| griddap | Subset | tabledap | Make A Graph | wms | files | Accessible | Title | Summary | FGDC | ISO 19115 | Info | Background Info | RSS | Institution | Dataset ID | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| log in | [Acropora cervicornis protein measurements: nutrient- and disease-exposed] - Acropora cervicornis protein measurements: nutrient- and disease-exposed from samples collected at Mote Marine Laboratory in situ nursery from June to July 2022 (Collaborative Research: Tracking the interacting roles of the environment, host genotype, and a novel Rickettsiales in coral disease susceptibility) | In the summer of 2022, 20 ramets each of the Acropora cervicornis genotypes ML-AC-36 and ML-AC-46 were collected from Mote's in situ coral nursery and subjected to three weeks of elevated nitrate, ammonium, and phosphate in the form of a slow-release fertilizer. The duration and concentration of this exposure were selected based on prior experiments conducted at Mote Marine Laboratory, which were found to alter microbial community profiles and growth rates in A. cervicornis (Klinges et al., 2022, 2023). During experiment, corals were held in 2-gallon aquaria with 5 corals per aquarium. As Mote Marine Laboratory's experimental aquarium system is plumbed into nearshore coastal water and thus has a higher nutrient load than the reef, an additional subset of 18 ramets of each genotype was collected from Mote's in situ coral nursery immediately prior to disease challenge to evaluate the impact of nearshore water on disease response. All ramets in the disease group were subjected to disease challenge in the form of a tissue homogenate produced from diseased fragments of random genotypes of A. cervicornis. Ramets in a comparative unexposed group were exposed to a homogenate produced from healthy fragments of random genotypes of A. cervicornis. To evaluate the effect of Aquarickettsia infection; nutrient enrichment; disease exposure; and the combination of these factors on coral immune function, a number of immune related proteins and antioxidants were measured using microplate assays from a total of 55 samples taken prior to disease exposure and following disease exposure as corals either resisted or developed disease. Total host protein and the antioxidant superoxide dismutase were found to be higher in healthy corals regardless of genotype or nutrient enrichment. Phenoloxidase and prophenoloxidase were higher in concentration in diseased samples compared to healthy samples. Disease-exposed but apparently healthy corals had higher superoxide dismutase, prophenoloxidase, and peroxidase activity than either healthy unexposed corals or diseased corals despite total protein concentrations that were lower than healthy unexposed corals.\n\ncdm_data_type = Other\nVARIABLES:\nFull_ID (unitless)\nDate (unitless)\nTP (mg)\nPOX (ΔAbs470nm)\nNormalized_POX (ΔAbs470nm/mg protein)\n... (17 more variables)\n | BCO-DMO | bcodmo_dataset_924466_v1 | ||||||||||||
| https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_764664 | https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_764664.graph | https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_764664/ | public | [Log_Kd] - Partition coefficient of 234Th, 233Pa, 210Pb, 210Po, and 7Be (Biopolymers as carrier phases for selected natural radionuclides (of Th, Pa, Pb, Po, Be) in diatoms and coccolithophores) | Laboratory studies were conducted to examine the sorption of selected radionuclides (234Th, 233Pa, 210Po, 210Pb, and 7Be) onto inorganic (pure silica and acid-cleaned diatom frustules) and organic (diatom cells with or without silica frustules) particles in natural seawater and the role of templating biomolecules and exopolymeric substances (EPS) extracted from the same species of diatom, Phaeodactylum tricornutum, in the sorption process. The range of partition coefficients (Kd, reported as logKd) of radionuclides between water and the different particle types was 4.78\\u20136.69 for 234Th, 5.23\\u20136.71 for 233Pa, 4.44\\u20135.86 for 210Pb, 4.47\\u20134.92 for 210Po, and 4.93\\u20137.23 for 7Be, similar to values reported for lab and field determinations. The sorption of all radionuclides was significantly enhanced in the presence of organic matter associated with particles, resulting in Kd one to two orders of magnitude higher than for inorganic particles only, with highest values for 7Be (logKd of 7.2). Results further indicate that EPS and frustule-embedded biomolecules in diatom cells are responsible for the sorption enhancement rather than the silica shell itself. By separating radiolabeled EPS via isoelectric focusing, we found that isoelectric points are radionuclide specific, suggesting that each radionuclide binds to specific biopolymeric functional groups, with the most efficient binding sites likely occurring in acid polysaccharides, iron hydroxides, and proteins. Further progress in evaluating the effects of diatom frustule\\u2013related biopolymers on binding, scavenging, and fractionation of radionuclides would require the application of molecular-level characterization techniques.\n\ncdm_data_type = Other\nVARIABLES:\nParticle_type (unitless)\nBe (kilogram per liter (kg/L))\nBe_sd (kilogram per liter (kg/L))\nPa (kilogram per liter (kg/L))\nPa_sd (kilogram per liter (kg/L))\nTh (kilogram per liter (kg/L))\nTh_sd (kilogram per liter (kg/L))\nPo (kilogram per liter (kg/L))\nPo_sd (kilogram per liter (kg/L))\nPb (kilogram per liter (kg/L))\nPb_sd (kilogram per liter (kg/L))\n | https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_764664/index.htmlTable | https://www.bco-dmo.org/dataset/764664
| https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_764664.rss | https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_764664&showErrors=false&email= | BCO-DMO | bcodmo_dataset_764664 |