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https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_926299_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_926299_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_926299_v1/ public [Microorganisms associated with doliolids] - Eukaryotic and prokaryotic microbial taxa retained by wild-caught doliolids collected during bloom events at three different shelf locations in the northern California Current system in June 2019. (Collaborative Research: Comparative feeding by gelatinous grazers on microbial prey) Doliolids have a unique ability to impact the marine microbial community through bloom events and high filtration rates. Their predation on large eukaryotic microorganisms is established and evidence of predation on smaller prokaryotic microorganisms is beginning to emerge. We studied the retention of both eukaryotic and prokaryotic microbial taxa by wild-caught doliolids in the northern California Current system. Doliolids were collected during bloom events identified at three different shelf locations with variable upwelling intensity.\n\ncdm_data_type = Other\nVARIABLES:\nbioproject_accession (unitless)\nbiosample_accession (unitless)\nsample_name (unitless)\nsra_sample_accession (unitless)\nsample_accession_title (unitless)\norganism_name (unitless)\norganism_taxonomy_id (unitless)\norganism_taxonomy_name (unitless)\nkeyword (unitless)\nbiosample_package (unitless)\ncollection_date (unitless)\ndepth (m)\nenv_broad_scale (unitless)\nenv_local_scale (unitless)\nenv_medium (unitless)\ngeo_loc_name (unitless)\nlatitude (Sampling_lat, degrees_north)\nlongitude (Sampling_lon, degrees_east)\nsize_frac (unitless)\nhost (unitless)\nsource_material_id (unitless)\nstatus (unitless)\n... (5 more variables)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_926299_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_926299_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_926299_v1/index.htmlTable https://www.bco-dmo.org/dataset/926299 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_926299_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_926299_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_926299_v1
log in [Microorganisms associated with pyrosomes] - High-throughput sequencing of the 16S rRNA gene, microscopy, and flow cytometry of pyrosome-associated microorganisms compared to seawater sampled during a Pyrosoma atlanticum bloom in the Northern California Current System in July 2018. (Collaborative Research: Comparative feeding by gelatinous grazers on microbial prey) Pyrosomes are widely distributed pelagic tunicates that have the potential to reshape marine food webs when they bloom. However, their grazing preferences and interactions with the background microbial community are poorly understood. The diversity, relative abundance, and taxonomy of pyrosome-associated microorganisms were compared to seawater during a Pyrosoma atlanticum bloom in the Northern California Current System using high-throughput sequencing of the 16S rRNA gene, microscopy, and flow cytometry.\n\ncdm_data_type = Other\nVARIABLES:\nbioproject_accession (unitless)\nbiosample_accession (unitless)\nsample_name (unitless)\nsra_sample_accession (unitless)\nsample_accession_title (unitless)\norganism_name (unitless)\norganism_taxonomy_id (unitless)\norganism_taxonomy_name (unitless)\nkeywords (unitless)\nbiosample_package (unitless)\ncollection_date (unitless)\nenv_broad_scale (unitless)\nenv_local_scale (unitless)\nenv_medium (unitless)\ngeo_loc_name (unitless)\nhost (unitless)\nlatitude (Sampling_lat, degrees_north)\nlongitude (Sampling_lon, degrees_east)\ndepth (m)\nhost_length (centimeter (cm))\nsource_material_id (unitless)\nstatus (unitless)\n... (20 more variables)\n BCO-DMO bcodmo_dataset_926093_v1
log in [Northern California Current Microorganisms] - 16S rRNA gene of microorganisms sampled along the Newport Hydrographic (NH) and Trinidad Head (TR) lines, in OR and CA in 2018 and 2019 (Collaborative Research: Comparative feeding by gelatinous grazers on microbial prey) The Northern California Current ecosystem is a productive system which supports major fisheries. To determine how the microbial community responds to variable upwelling, we examined the 16S rRNA gene of microorganisms from two size fractions, 0.2-1.6µm and greater than 1.6µm along the Newport Hydrographic (NH) and Trinidad Head (TR) lines, in OR and CA.\n\ncdm_data_type = Other\nVARIABLES:\nbioproject_accession (unitless)\nbiosample_accession (unitless)\nmessage (unitless)\nsample_name (unitless)\norganism (unitless)\ncollection_date (unitless)\ndepth (m)\nenv_broad_scale (unitless)\nenv_local_scale (unitless)\nenv_medium (unitless)\ngeo_loc_name (unitless)\nlatitude (Sampling_lat, degrees_north)\nlongitude (Sampling_lon, degrees_east)\nsize_frac (unitless)\nsra_run_accession (unitless)\nsra_study_accession (unitless)\nobject_status (unitless)\nlibrary_ID (unitless)\ntitle (unitless)\nlibrary_strategy (unitless)\nlibrary_source (unitless)\nlibrary_selection (unitless)\nlibrary_layout (unitless)\nplatform (unitless)\n... (5 more variables)\n BCO-DMO bcodmo_dataset_926850_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_952988_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_952988_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_952988_v1/ public [PMP counts in sponges and seawater from Panama] - Potential microplastic counts of 6 common Caribbean spong species collected June 21, 2019 in Bocas del Toro, Panama (Collaborative Research: Investigations into microbially mediated ecological diversification in sponges) Microplastics (MP) are now considered ubiquitous across global aquatic environments. The ingestion of MP by fish and other marine vertebrates is well studied, but the ingestion of MP by marine invertebrates is not. Sponges (Phylum Porifera) are particularly understudied when it comes to MP ingestion, even though they are widely distributed across benthic habitats, can process large volumes of seawater, and can retain small particles within their water filtration systems. This study examines the presence of potential MP (PMP) in wild marine sponges and seawater collected in Bocas del Toro, Panamá. Subsurface seawater and tissue from six common Caribbean sponge species was collected in Saigon Bay, a heavily impacted, shallow-water coral reef. Seawater samples were filtered onto glass fiber filters to retain any PMP present and sponge tissue was digested with bleach, heated and filtered. Filters were examined using fluorescence microscopy to quantify PMP. An average of 107 ± 25 PMP L–1 was detected in seawater from Saigon Bay with particles ranging in size between 10 μm and ~3,000 μm. The number of PMP found in sponge tissue ranged between 6 ± 4 and 169 ± 71 PMP g–1 of dry tissue. Most particles found in sponge samples were very small (10–20 μm), but fibers greater than 5,000 μm were detected. Our results indicate that PMP exists within the tissues of the sponges we studied, but future studies should confirm the presence of MP in sponges using chemical analysis. Most importantly, the discrepancy between low levels of PMP in our sponge samples and high levels in the surrounding seawater highlights the potential for sponges to resist and/or egest MP. Finally, we provide a critical evaluation of our methods to improve their use in future MP work with benthic marine organisms.\n\ncdm_data_type = Other\nVARIABLES:\nSample (unitless)\nSpecies (unitless)\nIndividual_number (unitless)\nSubsample_set (unitless)\nEmpty_Mass_g (gram)\nFull_Mass_g (gram)\nDry_Mass_g (gram)\nNumberPMP (number)\nCorrected_Net_NumberPMP (number)\n... (4 more variables)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_952988_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_952988_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_952988_v1/index.htmlTable https://osprey.bco-dmo.org/dataset/952988 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_952988_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_952988_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_952988_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_955389_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_955389_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_955389_v1/ public [PMP size in sponges and seawater from Panama] - Potential microplastic sizes of 6 common Caribbean spong species collected June 21, 2019 in Bocas del Toro, Panama (Collaborative Research: Investigations into microbially mediated ecological diversification in sponges) Microplastics (MP) are now considered ubiquitous across global aquatic environments. The ingestion of MP by fish and other marine vertebrates is well studied, but the ingestion of MP by marine invertebrates is not. Sponges (Phylum Porifera) are particularly understudied when it comes to MP ingestion, even though they are widely distributed across benthic habitats, can process large volumes of seawater, and can retain small particles within their water filtration systems. This study examines the presence of potential MP (PMP) in wild marine sponges and seawater collected in Bocas del Toro, Panamá. Subsurface seawater and tissue from six common Caribbean sponge species was collected in Saigon Bay, a heavily impacted, shallow-water coral reef. Seawater samples were filtered onto glass fiber filters to retain any PMP present and sponge tissue was digested with bleach, heated and filtered. Filters were examined using fluorescence microscopy to quantify PMP. An average of 107 ± 25 PMP L–1 was detected in seawater from Saigon Bay with particles ranging in size between 10 μm and ~3,000 μm. The number of PMP found in sponge tissue ranged between 6 ± 4 and 169 ± 71 PMP g–1 of dry tissue. Most particles found in sponge samples were very small (10–20 μm), but fibers greater than 5,000 μm were detected. Our results indicate that PMP exists within the tissues of the sponges we studied, but future studies should confirm the presence of MP in sponges using chemical analysis. Most importantly, the discrepancy between low levels of PMP in our sponge samples and high levels in the surrounding seawater highlights the potential for sponges to resist and/or egest MP. Finally, we provide a critical evaluation of our methods to improve their use in future MP work with benthic marine organisms.\n\ncdm_data_type = Other\nVARIABLES:\nSample_type (unitless)\nSample_replicate (unitless)\nLength_um (micrometers (um))\nWidth_um (micrometers (um))\nlatitude (Sampling_lat, degrees_north)\nlongitude (Sampling_lon, degrees_east)\nsampling_date (unitless)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_955389_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_955389_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_955389_v1/index.htmlTable https://osprey.bco-dmo.org/dataset/955389 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_955389_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_955389_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_955389_v1
log in [Salp and pteropod associated microorganisms] - Salp and pteropod associated microorganisms from the  Western Edge of the Gulf Stream sampled in September 2019. (Collaborative Research: Comparative feeding by gelatinous grazers on microbial prey) Microbial mortality impacts the structure of food webs, carbon flow, and the interactions that create dynamic patterns of abundance across gradients in space and time in diverse ecosystems. In the oceans, estimates of microbial mortality by viruses, protists, and small zooplankton do not account fully for observations of loss, suggesting the existence of underappreciated mortality sources. We examined how ubiquitous mucous mesh feeders (i.e. gelatinous zooplankton) could contribute to microbial mortality in the open ocean. We coupled capture of live animals by blue-water diving to sequence-based approaches to measure the enrichment and selectivity of feeding by two coexisting mucous grazer taxa (pteropods and salps) on numerically dominant marine prokaryotes. We show that mucous mesh grazers consume a variety of marine prokaryotes and select between coexisting lineages and similar cell sizes. We show that Prochlorococcus may evade filtration more than other cells and that planktonic archaea are consumed by macrozooplanktonic grazers. Discovery of these feeding relationships identifies a new source of mortality for Earth's dominant marine microbes and alters our understanding of how top-down processes shape microbial community and function.\n\ncdm_data_type = Other\nVARIABLES:\nbioproject_accession (unitless)\nbiosample_accession (unitless)\nmessage (unitless)\nsample_name (unitless)\nsample_title (unitless)\norganism (unitless)\ncollection_date (unitless)\ndepth_f (Depth, feet)\nenv_broad_scale (unitless)\nenv_local_scale (unitless)\nenv_medium (unitless)\ngeo_loc_name (unitless)\nlatitude (Sampling_lat, degrees_north)\nlongitude (Sampling_lon, degrees_east)\n... (15 more variables)\n BCO-DMO bcodmo_dataset_926841_v1

 
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