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log in [Acropora cervicornis protein measurements: nutrient- and disease-exposed] - Acropora cervicornis protein measurements: nutrient- and disease-exposed from samples collected at  Mote Marine Laboratory in situ nursery from June to July 2022 (Collaborative Research: Tracking the interacting roles of the environment, host genotype, and a novel Rickettsiales in coral disease susceptibility) In the summer of 2022, 20 ramets each of the Acropora cervicornis genotypes ML-AC-36 and ML-AC-46 were collected from Mote's in situ coral nursery and subjected to three weeks of elevated nitrate, ammonium, and phosphate in the form of a slow-release fertilizer. The duration and concentration of this exposure were selected based on prior experiments conducted at Mote Marine Laboratory, which were found to alter microbial community profiles and growth rates in A. cervicornis (Klinges et al., 2022, 2023). During experiment, corals were held in 2-gallon aquaria with 5 corals per aquarium. As Mote Marine Laboratory's experimental aquarium system is plumbed into nearshore coastal water and thus has a higher nutrient load than the reef, an additional subset of 18 ramets of each genotype was collected from Mote's in situ coral nursery immediately prior to disease challenge to evaluate the impact of nearshore water on disease response. All ramets in the disease group were subjected to disease challenge in the form of a tissue homogenate produced from diseased fragments of random genotypes of A. cervicornis. Ramets in a comparative unexposed group were exposed to a homogenate produced from healthy fragments of random genotypes of A. cervicornis.  To evaluate the effect of Aquarickettsia infection; nutrient enrichment; disease exposure; and the combination of these factors on coral immune function, a number of immune related proteins and antioxidants were measured using microplate assays from a total of 55 samples taken prior to disease exposure and following disease exposure as corals either resisted or developed disease. Total host protein and the antioxidant superoxide dismutase were found to be higher in healthy corals regardless of genotype or nutrient enrichment. Phenoloxidase and prophenoloxidase were higher in concentration in diseased samples compared to healthy samples. Disease-exposed but apparently healthy corals had higher superoxide dismutase, prophenoloxidase, and peroxidase activity than either healthy unexposed corals or diseased corals despite total protein concentrations that were lower than healthy unexposed corals.\n\ncdm_data_type = Other\nVARIABLES:\nFull_ID (unitless)\nDate (unitless)\nTP (mg)\nPOX (ΔAbs470nm)\nNormalized_POX (ΔAbs470nm/mg protein)\n... (17 more variables)\n BCO-DMO bcodmo_dataset_924466_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_908572_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_908572_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_908572_v1/ public [CDOM] - Chromophoric dissolved organic matter (CDOM) from two microcosm incubation experiments conducted under three light treatments using water originating from West Bay of the Neuse River Estuary, North Carolina USA in 2021 and 2022 (Bacteria as Biosensors of Carbon and Energy Flow in Marine Ecosystems: Quantitative Links Between Substrates, Transcripts, and Metabolism) Chromophoric dissolved organic matter (CDOM) was collected for two microcosm incubation experiments. Sample water originated from West Bay of the Neuse River Estuary, North Carolina USA in 2021 and 2022. The microcosms were 60 liters, conducted in biological duplicates under three light treatment incubations: 12-hour light-dark cycle of photosynthetically active radiation (PAR), 12-hour light-dark cycle of UV-B radiation, or darkness. Samples were collected from the microcosms in duplicate every few days for over one month to examine how light and the resulting microbial activity altered the dissolved organic carbon (DOC) pool over time. Absorbance spectra of 0.2 micron filtered estuarine water was measured from 190 - 1100 nanometers (nm) on a Genesys 10S UV-Vis spectrophotometer for the calculation of absorbance coefficients, spectral slopes, and slope ratio which describe the organic matter complexity as it develops throughout the incubations.\n\ncdm_data_type = Other\nVARIABLES:\nExperiment_Name (unitless)\nStart_date (unitless)\nTreatment (unitless)\nIncubation_day (days)\nTank_ID (unitless)\nnm (nanometers (nm))\nabsp_coef (unitless)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_908572_v1/index.htmlTable https://www.bco-dmo.org/dataset/908572 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_908572_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_908572_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_908572_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_944277_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_944277_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_944277_v1/ public [Dissolved Inorganic Nutrients] - Dissolved inorganic nutrients from two microcosm incubation experiments conducted on sample water collected from West Bay of the Neuse River Estuary, North Carolina USA in 2021 and 2022 (Bacteria as Biosensors of Carbon and Energy Flow in Marine Ecosystems: Quantitative Links Between Substrates, Transcripts, and Metabolism) Dissolved inorganic nutrients were collected for two microcosm incubation experiments. Sample water originated from West Bay of the Neuse River Estuary, North Carolina USA in 2021 and 2022. The microcosms were 60 liters, conducted in biological duplicates under three light treatment incubations: 12-hour light-dark cycle of photosynthetically active radiation (PAR), 12-hour light-dark cycle of UV-B radiation, or darkness. Samples were collected from the microcosms in duplicate every few days for over one month to examine how light and the resulting microbial activity altered the concentrations of dissolved inorganic nutrients (total dissolved nitrate and nitrite or NOx, ammonium or NH4, and soluble reactive phosphorus or SRP) over time. Concentrations of these inorganic nutrients were quantified via colorimetric assays on a Genesys 10S UV-Vis spectrophotometer or Tecan Infinite 200Pro plate reader.\n\ncdm_data_type = Other\nVARIABLES:\nExperiment (unitless)\nDate_Start (unitless)\nIncubation_Day (days)\nTreatment (unitless)\nTank_ID (unitless)\nReplicate (unitless)\nNOx (micromolar (uM))\nNH4 (micromolar (uM))\nSRP (micromolar (uM))\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_944277_v1/index.htmlTable https://www.bco-dmo.org/dataset/944277 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_944277_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_944277_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_944277_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_908475_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_908475_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_908475_v1/ public [Dissolved Organic Carbon] - Bulk dissolved organic carbon (DOC) from two microcosm incubation experiments conducted under three light treatments using water originating from West Bay of the Neuse River Estuary, North Carolina USA in 2021 and 2022 (Bacteria as Biosensors of Carbon and Energy Flow in Marine Ecosystems: Quantitative Links Between Substrates, Transcripts, and Metabolism) Bulk dissolved organic carbon (DOC) was collected from two microcosm incubation experiments. Sample water originated from West Bay of the Neuse River Estuary, North Carolina USA in 2021 and 2022. The microcosms were 60 liters, conducted in biological duplicates under three light treatment incubations: 12-hour light-dark cycle of photosynthetically active radiation (PAR), 12-hour light-dark cycle of UV-B radiation, or darkness. Samples were collected from the microcosms in duplicate every few days for over one month to examine how light and the resulting microbial activity altered the DOC pool over time.\n\ncdm_data_type = Other\nVARIABLES:\nExperiment_Name (unitless)\nStart_date (unitless)\nTreatment (unitless)\nIncubation_day (days)\nTank_ID (unitless)\nDOC_uM (micromoles per liter (umol L-1))\nDOC_Technical_sd (micromoles per liter (umol L-1))\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_908475_v1/index.htmlTable https://www.bco-dmo.org/dataset/908475 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_908475_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_908475_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_908475_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_925050_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_925050_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_925050_v1/ public [Microbial Cellular Abundance Epifluorescent Microscopy] - Microbial cellular abundance growth response through epifluorescent microscopy from the Neuse River Estuary, North Carolina USA from 2021-2022 (Bacteria as Biosensors of Carbon and Energy Flow in Marine Ecosystems: Quantitative Links Between Substrates, Transcripts, and Metabolism) Microbial cellular abundance was enumerated for two microcosm incubation experiments to track the growth response of the microbial community. This dataset targets bacterial and phytoplankton abundance through epiflorescent microscopy. Sample water originated from the West Bay of the Neuse River Estuary, North Carolina USA in 2021 and 2022. The microcosms were 60-L, conducted in biological duplicates under three light treatment incubations: 12 h light-dark cycle of photosynthetically active radiation (PAR), 12 h light-dark cycle of UV-B radiation, or darkness. Unfiltered sample water was stained with SYBR Green I (Fall 2021 experiment) or DAPI (Spring 2022 experiment) for enumeration at 60x for bacterial and cyanobacterial abundance. Autofluorescence from excitation at 620 nm and 60x was used to enumerate small phytoplankton and cyanobacteria. This dataset highlights patterns in microbial growth across treatments over the course of the incubation and is used to generate cell-specific, normalized rates for associated datasets in the project.\n\ncdm_data_type = Other\nVARIABLES:\nExperiment_Name (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\nDate (unitless)\nIncubation_Day (days)\nTreatment (unitless)\nTank_ID (unitless)\nMethod (unitless)\nStain (unitless)\nExcitation (nanometers (nm))\nMagnification (unitless)\nSize_Fraction (micrometers (um))\ncells_per_L_avg (cells L-1)\ncells_per_L_sd (cells L-1)\nratio_620_tot (unitless)\nratio_err (unitless)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_925050_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_925050_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_925050_v1/index.htmlTable https://www.bco-dmo.org/dataset/925050 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_925050_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_925050_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_925050_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_908626_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_908626_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_908626_v1/ public [Microbial Respiration] - Microbial respiration from microcosm experiments conducted under three light treatments using water originating from West Bay of the Neuse River Estuary, North Carolina USA in 2021 and 2022 (Bacteria as Biosensors of Carbon and Energy Flow in Marine Ecosystems: Quantitative Links Between Substrates, Transcripts, and Metabolism) Microbial respiration assays were conducted for two microcosm incubation experiments. Sample water originated from West Bay of the Neuse River Estuary, North Carolina USA in 2021 and 2022. The microcosms were 60-liters, conducted in biological duplicates under three light treatment incubations: 12-hour light-dark cycle of photosynthetically active radiation (PAR), 12-hour light-dark cycle of UV-B radiation, or darkness. Respiration assays of the unfiltered and 5-micron filtered community were initiated every few days using foil-membrane optodes to examine light effects on community respiration.\n\ncdm_data_type = Other\nVARIABLES:\nExperiment_Name (unitless)\nDate (unitless)\nTime_EDT (unitless)\nIncubation_day (days)\nTreatment (unitless)\nTank_ID (unitless)\nSize_fraction_um (micrometers (um))\nrespiration_uM_O2_d_1 (micromoles O2 per day (uM O2 d-1))\nrespiration_uM_C_d_1 (micromoles C per day (uM C d-1))\nr2 (unitless)\nRSE (unitless)\nNo_pts (unitless)\nISO_DateTime_Local (unitless)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_908626_v1/index.htmlTable https://www.bco-dmo.org/dataset/908626 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_908626_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_908626_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_908626_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_733238 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_733238.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_733238/ public [Rapid Light Curves_PAM] - Measurements of fluorescence of photosystem II in Plocamium cartilagineum under various and pCO2 and temperature conditions (Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae) This dataset includes photosynthetic pigment concentrations in Plocamium cartilagineum grown under various temperatures and CO2 levels, from July 2014 to February 2015. The parameters reported are: the concentrations of Chlorophyll a, Phycoerythrin, Phycocyanin, Allophycocyanin, and total Phycobiliprotein, and the ratio of total Phycobiliprotein to Chlorophyll A.\n\ncdm_data_type = Other\nVARIABLES:\nTrial (unitless)\nTank_ID (unitless)\npCO2 (P CO2, atm)\nTemp (Temperature, degrees Celsius (°C))\nPlant_ID (unitless)\nID (unitless)\nWeek (unitless)\ndate (unitless)\nlight (micromole photons/meter^2/second ( µmole photons . m^-2 . s^-1))\nF (unitless)\nFm (unitless)\nYield (unitless)\nrETR (R ETR, unitless)\nqP (Q P, unitless)\nqN (Q N, unitless)\nNPQ (unitless)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_733238/index.htmlTable https://www.bco-dmo.org/dataset/733238 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_733238.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_733238&showErrors=false&email= BCO-DMO bcodmo_dataset_733238

 
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