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https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_968956_v1/ public [AE2413 FlaPS bulk] - Polysaccharide hydrolase activities from water samples collected at various sites under varying hydrostatic pressures in the Western North Atlantic aboard R/V Atlantic Explorer cruise AE2413 in May 2024 (Collaborative Research: Pressure effects on microbially-catalyzed organic matter degradation in the deep ocean) Heterotrophic bacteria and archaea (here: microbes) are critical drivers of the ocean's biogeochemical cycles, active throughout the depth of the ocean. Their capabilities and limitations help determine the rates and locations at which carbon and nutrients are regenerated, as well as the extent to which organic matter is preserved (Hedges 1992). In the deep ocean, at bathy- and abyssopelagic depths (ca. 1000-6000m), these communities are dependent upon the sinking flux of particulate organic matter (POM) from the surface ocean (Bergauer et al. 2018). This dependence means that heterotrophic microbial communities must produce the extracellular enzymes required to solubilize and hydrolyze high molecular weight (HMW) POM to sizes substrates suitable for cellular uptake. A recent global-scale investigation of deep-sea microbes in fact found that the genetic potential for exported (extracellular) enzymes among bacteria in deep waters was far greater than for communities in surface or mesopelagic waters (Zhao et al. 2020). We have new evidence that a substantial fraction of bacteria in bottom water from the North Atlantic Ocean use a specialized set of extracellular enzymes to rapidly take up HMW polysaccharides (Giljan et al. 2021), a substrate processing mechanism that would not be detected with the low molecular weight substrates used in most prior studies of microbial activity in the deep ocean (Nagata et al. 2010).\n \nThrough our collaboration with the Danish Center for Hadal Research, we were able to use pressurization systems and in situ specialized equipment to investigate the effects of pressures characteristic of bathy- and abyssopelagic depths on microbial communities and their extracellular enzymes in the open North Atlantic Ocean.  \n \nHere we present the measurement of polysaccharide hydrolase activities above that measured for killed controls from various sites under varying hydrostatic pressures in the Western North Atlantic aboard R/V Atlantic Explorer, cruise AE2413 in May 2024.\n\ncdm_data_type = Other\nVARIABLES:\ndeployment (unitless)\nstation (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\n... (26 more variables)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_968956_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_968956_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_968956_v1/index.htmlTable https://osprey.bco-dmo.org/dataset/968956 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_968956_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_968956_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_968956_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_963393_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_963393_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_963393_v1/ public [Combined Selfish Bacteria Cell Counts 2023-2024] - Cell counts exhibiting 'Selfish' uptake in the Western North Atlantic, in Danish Coastal Seawater, and Abyssopelagic Waters off the Eastern Coast of Japan under varying hydrostatic pressures, 2023-2024 (Collaborative Research: Pressure effects on microbially-catalyzed organic matter degradation in the deep ocean) Heterotrophic bacteria and archaea (here: microbes) are critical drivers of the ocean's biogeochemical cycles, active throughout the depth of the ocean. Their capabilities and limitations help determine the rates and locations at which carbon and nutrients are regenerated, as well as the extent to which organic matter is preserved (Hedges 1992). In the deep ocean, at bathy- and abyssopelagic depths (ca. 1000-6000m), these communities are dependent upon the sinking flux of particulate organic matter (POM) from the surface ocean (Bergauer et al. 2018). This dependence means that heterotrophic microbial communities must produce the extracellular enzymes required to solubilize and hydrolyze high molecular weight (HMW) POM to sizes substrates suitable for cellular uptake. A recent global-scale investigation of deep-sea microbes in fact found that the genetic potential for exported (extracellular) enzymes among bacteria in deep waters was far greater than for communities in surface or mesopelagic waters (Zhao et al. 2020). We have new evidence that a substantial fraction of bacteria in bottom water from the North Atlantic Ocean use a specialized set of extracellular enzymes to rapidly take up HMW polysaccharides (Giljan et al. 2022), a substrate processing mechanism that would not be detected with the low molecular weight substrates used in most prior studies of microbial activity in the deep ocean (Nagata et al. 2010).\n \nThrough our collaboration with the Danish Center for Hadal Research, we were able to use pressurization systems and in situ specialized equipment to investigate the effects of pressures characteristic of bathy- and abyssopelagic depths on microbial communities and their extracellular enzymes in the open North Atlantic Ocean, in Danish Coastal Seawater, and abyssopelagic waters off the Eastern Coast of Japan. \n \nHere we present, in collaboration with colleagues from the Max Planck Institute for Marine Microbiology, the detection and quantification of microbial cells exhibiting selfish uptake behavior of fluorescently-labeled HMW polysaccharides. This dataset includes sample collection metadata, environmental variables, experimental variables, the number of cells detected exhibiting 'selfish' uptake, and total cellular abundance.\n\ncdm_data_type = Other\nVARIABLES:\ndeployment (unitless)\n... (22 more variables)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_963393_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_963393_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_963393_v1/index.htmlTable https://osprey.bco-dmo.org/dataset/963393 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_963393_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_963393_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_963393_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_652849 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_652849.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_652849/ public [Coral-bacterioplankton data from mesocosm experiments] - Bacterioplankton data from coral and coral mucus aquaria experiments conducted at Bermuda Institute of Ocean Sciences in 2013 (Fundamental Coral-Microbial Associations) Bacterioplankton data from coral and coral mucus aquaria experiments conducted at Bermuda Institute of Ocean Sciences in 2013\n\ncdm_data_type = Other\nVARIABLES:\ntreatment (unitless)\ntreatment_description (unitless)\ntank (unitless)\nmesocosm (unitless)\ntimepoint_name (days)\ntimepoint_days (days)\ntemp (Temperature, celsius)\nsalinity (Sea Water Practical Salinity, ppt)\nDAPI_count (cells per milliliter)\naccession_number (unitless)\nnonPigmentPico (Non Pigment Pico, cells per milliliter)\nsynechococcus (cells per milliliter)\npicoeukaryote (cells per milliliter)\nlowHeterotrophicBac (Low Heterotrophic Bac, cells per milliliter)\nhighHeterotrophicBac (High Heterotrophic Bac, cells per milliliter)\nSAR11 (cells per milliliter)\nrhodobacteracea (cells per milliliter)\nalteromonas (cells per milliliter)\nvibrio (cells per milliliter)\nthaumarchaeota (cells per milliliter)\neuryarchaeota (cells per milliliter)\nP04 (micromoles)\nN03_N02 (micromoles)\nSi04 (micromoles)\nN02 (micromoles)\nNH4 (Mole Concentration Of Ammonium In Sea Water, micromoles)\n... (9 more variables)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_652849/index.htmlTable https://www.bco-dmo.org/dataset/652849 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_652849.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_652849&showErrors=false&email= BCO-DMO bcodmo_dataset_652849
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_946056_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_946056_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_946056_v1/ public [Dissolved organic carbon lability] - Water column dissolved organic carbon (DOC) reactivity along the York River Estuary (YRE) from surface samples collected in October 2018 and February, April, and July 2019 (Alteration of carbon fluxes by intense phytoplankton blooms in a microtidal estuary) Data were collected to evaluate spatiotemporal variations in, and environmental controls on, water column dissolved organic carbon (DOC) reactivity along the York River Estuary (YRE), a temperate microtidal sub-estuary of the Chesapeake Bay in southeastern Virginia. Data were also collected as part of the larger, NSF-funded project (NSF BIO-OCE #1737258) entitled \"Alteration of carbon fluxes by intense phytoplankton blooms in a micro tidal estuary.\" Surface water column samples were collected in October 2018 and February, April, and July 2019 from three locations along the YRE estuarine salinity gradient. Filtered water was inoculated and incubated in the dark at in situ York River water temperatures, and sub-sampled at the onset of the experiment (T0), one day following onset (T1), and weekly for 28 days thereafter (T7, T14, T21, T28). Samples were analyzed for DOC concentrations.\n\ncdm_data_type = Other\nVARIABLES:\nSite (unitless)\nlatitude (degrees_north)\nlongitude (Long, degrees_east)\nMonth_Collected (unitless)\nDate_Sampled (unitless)\nTimepoint_days (days)\nDOC_uM (micromolar (umol/l))\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_946056_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_946056_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_946056_v1/index.htmlTable https://osprey.bco-dmo.org/dataset/946056 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_946056_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_946056_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_946056_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_963382_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_963382_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_963382_v1/ public [Polysaccharide Hydrolase Activities under Varying Hydrostatic Pressures near Helsingor in 2023] - Polysaccharide Hydrolase Activities in Danish Coastal Seawater and Sediments under Varying Hydrostatic Pressures on samples collected in September 2023 (Collaborative Research: Pressure effects on microbially-catalyzed organic matter degradation in the deep ocean) The potential of the seawater or sedimentary microbial community to hydrolyze seven high-molecular-weight polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan, laminarin, mannan, pullulan, and xylan) was investigated in a coastal station off the coast of Helsingor, Denmark. This investigation was part of the larger project to understand pressure effects on enzymatic activity. These samples were collected in September 2023 at a coastal station off the coast of Helsingor, Denmark, at a depth of 20 meters. \n\nThrough our collaboration with the Danish Center for Hadal Research, we were able to use pressurization systems and in situ specialized equipment to investigate the effects of pressures characteristic of bathy- and abyssopelagic depths on microbial communities and their extracellular enzymes in the open North Atlantic Ocean.   \n\nThis dataset contains metadata on sample collection, environmental conditions, sample types and treatments, incubation conditions, substrate types, and kill-corrected enzymatic hydrolysis rates across timepoints.\n\ncdm_data_type = Other\nVARIABLES:\nlatitude (degrees_north)\nlongitude (degrees_east)\ntime (Iso_datetime_utc, seconds since 1970-01-01T00:00:00Z)\ndate (unitless)\ntime_local_CEST (unitless)\ndepth (Depth_actual, m)\nsample_type (unitless)\nin_situ_T (degrees Celsius)\nin_situ_S (psu)\nincubation_T (degrees Celsius)\nunamended_amended (unitless)\npressure (MPa)\nsubstrate (unitless)\n... (8 more variables)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_963382_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_963382_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_963382_v1/index.htmlTable https://osprey.bco-dmo.org/dataset/963382 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_963382_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_963382_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_963382_v1

 
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