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| griddap | Subset | tabledap | Make A Graph | wms | files | Accessible | Title | Summary | FGDC | ISO 19115 | Info | Background Info | RSS | Institution | Dataset ID | |
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| log in | [Physicochemical Experimental Data and PcaFV Loads in Experiment] - Dynamics of the Apostichopus californicus-associated flavivirus under suboxic conditions and organic matter amendment in mesocosm experiment (Exploring the role of boundary layer microbial remineralization in flavivirus-host dynamics) | Flaviviruses cause some of the most detrimental vertebrate diseases, yet little is known of their impacts on invertebrates. Microbial activities at the animal-water interface are hypothesized to influence viral replication and possibly contribute to pathology of echinoderm wasting diseases due to hypoxic stress. We assessed the impacts of enhanced microbial production and suboxic stress on Apostichopus californicus (urn:lsid:marinespecies.org:taxname:529363) associated flavivirus (PcaFV) load in a mesocosm experiment. Organic matter amendment and suboxic stress resulted in lower PcaFV load, which also correlated negatively with animal mass loss and microbial activity at the animal-water interface. These data suggest that PcaFV replication and persistence was best supported in healthier specimens. Our results do not support the hypothesis that suboxic stress or microbial activity promote PcaFV replication, but rather that PcaFV appears to be a neutral or beneficial symbiont of Apostichopus californicus. This primary dataset contains per-specimen measurements including experimental treatments, microbial productivity (animal surface and mesocosm water), body wall lipid/protein content, animal mass loss, tissue-specific PcaFV viral loads at initial and final timepoints, and collection metadata. The supplemental file provides time-series measurements of dissolved oxygen and temperature in mesocosms, indexed by tank number, treatment, and timestamp.\n\ncdm_data_type = Other\nVARIABLES:\nSpecimen_No (unitless)\nTreatment (unitless)\nAnimal_Surf_Productivity (unitless)\nMesocosm_Water_Productivity (unitless)\nTissue_Lipid_pct (percent)\nTissue_Protein_Concentration (ug/mL)\nAnimal_Mass_loss (percent per day)\nTube_Foot_PcaFV_Load_Day_0 (copies / ng RNA)\nTube_Foot_PcaFV_Load_Day_7 (copies / ng RNA)\nResp_Tree_PcaFV_Load_Day_7 (copies / ng RNA)\nGonad_PcaFV_Load_Day_7 (copies / ng RNA)\nIntestine_PcaFV_Load_Day_7 (copies / ng RNA)\nDate_of_Animal_Sampling (unitless)\nDate_of_Specimen_Collection (unitless)\n | BCO-DMO | bcodmo_dataset_984803_v1 |