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log in [Genetic analyses and microsatellite characterization of new zooxanthellae species for Butler et al. (2023)] - Genetic analyses and microsatellite characterization for formal recognition of new species of host-generalist species of dinoflagellate (Cladocopium, Symbiodiniaceae) mutualistic with Indo-Pacific reef corals collected from 2003 through 2015 (Collaborative Research: Stability, flexibility, and functionality of thermally tolerant coral symbioses) These genetic data were used to justify the formal characterization of new species of ‘ecological generalists' zooxanthellae, the dinoflagellate symbionts living in the tissues of numerous marine animals including corals. Reef-building corals are dependent on endosymbiotic dinoflagellates (Family: Symbiodiniaceae) for their survival and growth but there is almost no taxonomy available for coral symbionts thus limiting research on them. These data include the sequences of genes originating from the nuclear genome, the mitochondrial genome as well as from the chloroplast genome. These sequences were analyzed independently and the resulting phylogenies from these analyses used to test for reciprocal monophyly, a way to verify the evolutionary divergence of two populations. Additionally, microsatellites were analyzed to test boundaries to genetic recombination indicated by the phylogenetic data.\n\ncdm_data_type = Other\nVARIABLES:\nSample_ID (unitless)\nSpecies (unitless)\nAphia_ID (unitless)\nSample_Origin (unitless)\nC1_22 (base pairs)\nC1_22_2 (base pairs)\nC1_25 (base pairs)\nC1_25_2 (base pairs)\nC1_24 (base pairs)\nC1_24_2 (base pairs)\nC1_05 (base pairs)\nC1_05_2 (base pairs)\nC1_16 (base pairs)\nC1_16_2 (base pairs)\nZ1_Spl_1 (base pairs)\nZ1_Spl_1_2 (base pairs)\nz78 (base pairs)\nz78_2 (base pairs)\n BCO-DMO bcodmo_dataset_957700_v1
log in [Pocillopora species ID from Recharge project] - Differential effects of nutrients and consumer pressure on sympatric cryptic coral species (Pocillopora spp.) in Moorea, French Polynesia sampled in November 2021. (Collaborative research: Coral community resilience: testing the role of hidden diversity in pocilloporid corals at Moorea) Cryptic species (evolutionarily distinct lineages that do not align with morphologically defined species) are being increasingly discovered but are poorly integrated into ecological theory. In particular, we still lack a useful understanding of if and how cryptic species differ in ways that affect community recovery from disturbances and responses to anthropogenic stressors, such as the removal of consumers and pollution from nutrients. \n\nOn coral reefs, nutrient pollution increases the growth of macroalgae that displaces corals. Reductions in herbivorous fishes reduce the suppression of macroalgae, while reductions in coralivorous fishes reduce predation on corals. An unresolved question is if and how cryptic coral species respond differently to these impacts, thereby differing in their ability to influence coral community dynamics and maintain coral dominance. Therefore, we assessed how the response of cryptic Pocillopora species over a period of three years following a simulated disturbance from a cyclone depended on the experimental reduction of fish consumer pressure and nutrient addition. After three years, five morphologically cryptic, but genetically distinct, Pocillopora species recruited to the reef. However, recruitment was dominated by two species: P. tuahiniensis (46%) and P. meandrina (43%). \n\nUnder ambient conditions, recruitment of P. tuahiniensis and P. meandrina was similar, but experimentally reducing consumer pressure increased recruitment of P. tuahiniensis by up to 73% and reduced recruitment of P. meandrina by up to 49%. In both species, nutrient enrichment increased recruitment and colony growth rates equally, but colonies of P. tuahiniensis grew faster, and were up to 25% larger after three years, than those of P. meandrina, and growth was unaffected by reduced consumer pressure. Predation by excavating corallivorous fish was higher for P. meandrina than for P. tuahiniensis, especially under nutrient enrichment. In contrast, polyp extension (an indicator of elevated heterotrophic feeding as well as susceptibility and attractiveness to corallivores) was lower for P. meandrina than for P. tuahiniensis, especially under low to medium consumer pressure. Overall, we uncovered ecological differences in the response of morphologically cryptic foundation species to two pervasive stressors on coral reefs. \n\nOur results demonstrate how cryptic species respond differently to key anthropogenic stressors, which may contribute to response diversity that can support ecological resilience or increase extinction risk.\n\ncdm_data_type = Other\nVARIABLES:\nlatitude (degrees_north)\n... (14 more variables)\n BCO-DMO bcodmo_dataset_964151_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_931469_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_931469_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_931469_v1/ public [Sanger Sequencing Analysis of Potexvirus PCR Products (Potex-5 and Potex-2RC)] - Sanger Sequencing Analysis of Potexvirus PCR Products (Potex-5 and Potex-2RC) collected during West Florida Coastal Surveys of Seagrass from Feb 2022 to Oct 2023 (Collaborative Research: VIDA Seagrass: Viral Infection Dynamics Among Seagrass) This dataset includes Sanger sequence references for 40 Thalassia testudinum samples collected during systematic seagrass surveys from multiple Florida sites, including Terra Ceia Aquatic Preserve, Tampa Bay seagrass sites S1T5 and S3T8 (Lassing Park), Panacea located in the Florida Panhandle, and Florida Keys sites including Bush Key, Garden Key, Marquesas Key, and Key West.\nWe investigated potexvirus distribution in seagrasses using a degenerate reverse transcription polymerase chain reaction (RT-PCR) assay originally designed to capture potexvirus diversity in terrestrial plants. The assay, which implements Potex-5 and Potex-2RC primers, successfully amplified a 584 nt RNA-dependent RNA polymerase (RdRp) fragment from TVX-infected seagrasses. Following validation, we screened 74 opportunistically collected, apparently healthy seagrass samples for potexviruses using this RT-PCR assay.\nPotexvirus PCR products were successfully generated from Thalassia testudinum samples. Sequences from these products are deposited in NCBI GenBank under the accession numbers OR827692-OR827705, OR854648, OR863396, OR879052-OR879056, and PP430548-PP430571.\n\ncdm_data_type = Other\nVARIABLES:\nsample (unitless)\nregion (unitless)\nsite (unitless)\ndate (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\nsample_host (unitless)\nAphia_id (unitless)\nGenbank_accession (unitless)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_931469_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_931469_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_931469_v1/index.htmlTable https://www.bco-dmo.org/dataset/931469 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_931469_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_931469_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_931469_v1

 
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