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BCO-DMO ERDDAP
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| griddap | Subset | tabledap | Make A Graph | wms | files | Accessible | Title | Summary | FGDC | ISO 19115 | Info | Background Info | RSS | Institution | Dataset ID | |
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| log in | [Genetic analyses and microsatellite characterization of new zooxanthellae species for Butler et al. (2023)] - Genetic analyses and microsatellite characterization for formal recognition of new species of host-generalist species of dinoflagellate (Cladocopium, Symbiodiniaceae) mutualistic with Indo-Pacific reef corals collected from 2003 through 2015 (Collaborative Research: Stability, flexibility, and functionality of thermally tolerant coral symbioses) | These genetic data were used to justify the formal characterization of new species of ‘ecological generalists' zooxanthellae, the dinoflagellate symbionts living in the tissues of numerous marine animals including corals. Reef-building corals are dependent on endosymbiotic dinoflagellates (Family: Symbiodiniaceae) for their survival and growth but there is almost no taxonomy available for coral symbionts thus limiting research on them. These data include the sequences of genes originating from the nuclear genome, the mitochondrial genome as well as from the chloroplast genome. These sequences were analyzed independently and the resulting phylogenies from these analyses used to test for reciprocal monophyly, a way to verify the evolutionary divergence of two populations. Additionally, microsatellites were analyzed to test boundaries to genetic recombination indicated by the phylogenetic data.\n\ncdm_data_type = Other\nVARIABLES:\nSample_ID (unitless)\nSpecies (unitless)\nAphia_ID (unitless)\nSample_Origin (unitless)\nC1_22 (base pairs)\nC1_22_2 (base pairs)\nC1_25 (base pairs)\nC1_25_2 (base pairs)\nC1_24 (base pairs)\nC1_24_2 (base pairs)\nC1_05 (base pairs)\nC1_05_2 (base pairs)\nC1_16 (base pairs)\nC1_16_2 (base pairs)\nZ1_Spl_1 (base pairs)\nZ1_Spl_1_2 (base pairs)\nz78 (base pairs)\nz78_2 (base pairs)\n | BCO-DMO | bcodmo_dataset_957700_v1 | ||||||||||||
| https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_931469_v1 | https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_931469_v1.graph | https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_931469_v1/ | public | [Sanger Sequencing Analysis of Potexvirus PCR Products (Potex-5 and Potex-2RC)] - Sanger Sequencing Analysis of Potexvirus PCR Products (Potex-5 and Potex-2RC) collected during West Florida Coastal Surveys of Seagrass from Feb 2022 to Oct 2023 (Collaborative Research: VIDA Seagrass: Viral Infection Dynamics Among Seagrass) | This dataset includes Sanger sequence references for 40 Thalassia testudinum samples collected during systematic seagrass surveys from multiple Florida sites, including Terra Ceia Aquatic Preserve, Tampa Bay seagrass sites S1T5 and S3T8 (Lassing Park), Panacea located in the Florida Panhandle, and Florida Keys sites including Bush Key, Garden Key, Marquesas Key, and Key West.\nWe investigated potexvirus distribution in seagrasses using a degenerate reverse transcription polymerase chain reaction (RT-PCR) assay originally designed to capture potexvirus diversity in terrestrial plants. The assay, which implements Potex-5 and Potex-2RC primers, successfully amplified a 584 nt RNA-dependent RNA polymerase (RdRp) fragment from TVX-infected seagrasses. Following validation, we screened 74 opportunistically collected, apparently healthy seagrass samples for potexviruses using this RT-PCR assay.\nPotexvirus PCR products were successfully generated from Thalassia testudinum samples. Sequences from these products are deposited in NCBI GenBank under the accession numbers OR827692-OR827705, OR854648, OR863396, OR879052-OR879056, and PP430548-PP430571.\n\ncdm_data_type = Other\nVARIABLES:\nsample (unitless)\nregion (unitless)\nsite (unitless)\ndate (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\nsample_host (unitless)\nAphia_id (unitless)\nGenbank_accession (unitless)\n | https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_931469_v1_fgdc.xml | https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_931469_v1_iso19115.xml | https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_931469_v1/index.htmlTable | https://www.bco-dmo.org/dataset/931469
| https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_931469_v1.rss | https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_931469_v1&showErrors=false&email= | BCO-DMO | bcodmo_dataset_931469_v1 |