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https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_928636_v1 https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_928636_v1/ public [Ca. Aquarickettsia rohweri transcriptomes] - Sampling and experimental metadata related to 'Candidatus' Aquarickettsia rohweri transcriptome data from host Acropora cervicornis colonies collected at Looe Key, Lower Florida Keys from Apr to Jun of 2019 (Collaborative Research: Tracking the interacting roles of the environment, host genotype, and a novel Rickettsiales in coral disease susceptibility) This dataset contains sampling and experimental metadata related to 'Candidatus' Aquarickettsia rohweri transcriptome sequences housed at the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) under BioProject PRJNA1048415. All host colonies (Acropora cervicornis genotype ML-50) were collected from the same location, the Mote Marine Laboratory in situ coral nursery in Looe Key, Lower Florida Keys between April and June of 2019.\n\nThe Rickettsiales-like bacterial parasite, 'Candidatus' Aquarickettsia rohweri (NCBI:txid2602574) is a ubiquitous coral symbiont that is strongly linked to coral disease susceptibility in staghorn coral, and is undergoing positive selection across the Caribbean. Although ‘Ca.' A. rohweri is a putative parasite, little is known about the activity of this bacterium in coral tissue. We performed a transcriptomic analyses of ‘Ca.' A. rohweri populations during a 6-week nutrient exposure experiment.  ‘Ca.' A. rohweri energy scavenging genes and those potentially involved during habitat transition are significantly upregulated during enrichment. Specifically, transcripts involved in signaling, virulence, two-component systems, and nutrient import genes are elevated under higher nutrients. These data support the predicted role of ‘Ca.' A. rohweri as a highly active nutrient-responsive A. cervicornis parasite and provide a glimpse at the mechanism of induced disease susceptibility while implicating nutrient exposure in its horizontal transmission.\n\ncdm_data_type = Other\nVARIABLES:\nSample_name (unitless)\nBioSample_Accession (unitless)\nSRA (unitless)\nBioProject (unitless)\nData_Type (unitless)\nScope (unitless)\nOrganism (unitless)\nCollected_Host_Organism (unitless)\nStrain (unitless)\nisolation_source (unitless)\nCollection_Year (unitless)\nCollection_date_note (unitless)\nTreatment (unitless)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_928636_v1/index.htmlTable https://osprey.bco-dmo.org/dataset/928636 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_928636_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_928636_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_928636_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_845039_v2 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_845039_v2.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_845039_v2/ public [Coral Associated Microbes on Mo'orean Coral Reefs] - Coral associated microbes on coral, sediment and water sampled from coral reefs in Mo'orea, French Polynesia in 2017 and 2018 (Collaborative Research: Viral Reefscapes: The Role of Viruses in Coral Reef Health, Disease, and Biogeochemical Cycling) Three species of coral, plus water and sediment, were sampled at 21 sites around the island of Mo'orea, French Polynesia during the dry and rainy seasons in 2017 and 2018.  Coral associated microbes (bacteria and archaea) were investigated and their community composition characterized through sequencing of the 16S rRNA gene.\n\ncdm_data_type = Other\nVARIABLES:\nBioSample_accession (unitless)\nSample_name (unitless)\nOrganism (unitless)\nCollection_date (unitless)\nDepth (meters (m))\nEnv_Local_Scale (unitless)\nGeo_Loc_Name (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\nHost (unitless)\nHost_Subject_ID (unitless)\nSequencing_Replicate (unitless)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_845039_v2_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_845039_v2_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_845039_v2/index.htmlTable https://www.bco-dmo.org/dataset/845039 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_845039_v2.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_845039_v2&showErrors=false&email= BCO-DMO bcodmo_dataset_845039_v2
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_785167.subset https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_785167 https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_785167/ public [Crassostrea virginica gonad MBD-BSseq] - Eastern oyster gonad methylation patterns in response to experimental ocean acidification (Collaborative Research: Does ocean acidification induce a methylation response that affects the fitness of the next generation in oysters?) Eastern oyster gonad methylation patterns in response to experimental ocean acidification at pCO2 levels 400 and 2800 ppm. Oysters were collected from an intertidal oyster reef in Plum Island Sound, MA, Gulf of Maine in mid-July 2016. This dataset includes GenBank BioProject PRJNA513384 metadata.\n\ncdm_data_type = Other\nVARIABLES:\nbioproject_accession (unitless)\nbiosample_accession (unitless)\nlibrary_ID (unitless)\ntitle (unitless)\nlibrary_strategy (unitless)\nlibrary_source (unitless)\nlibrary_selection (unitless)\nlibrary_layout (unitless)\nplatform (unitless)\ninstrument_model (unitless)\ndesign_description (unitless)\nfiletype (unitless)\nfilename (unitless)\nfilename2 (unitless)\nMBD_cv_id (unitless)\npCO2_treatment (P CO2 Treatment, ppm)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_785167/index.htmlTable https://www.bco-dmo.org/dataset/785167 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_785167.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_785167&showErrors=false&email= BCO-DMO bcodmo_dataset_785167
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_930084_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_930084_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_930084_v1/ public [Kāneʻohe Bay Time-series - microbial community] - Flow cytometry, 16S rRNA gene amplicons, chlorophyll a, and surface seawater measurements  taken between August 2017 to June 2019 Kāneʻohe Bay, Oʻahu, Hawaiʻi (Population genomics and ecotypic divergence in the most dominant lineage of marine bacteria) These data include temperature, pH, salinity, chlorophyll a concentrations, cellular abundances of Prochlorococcus, Synechococcus, photosynthetic picoeukaryotes, and heterotrophic bacteria, and 16S ribosomal RNA gene amplicon libraries from 200 surface seawater samples collected as part of the Kāneʻohe Bay Time-series (KByT). Near-monthly sampling of surface seawater was conducted between August 2017 to June 2019 at 10 sites within coastal waters of Kāneʻohe Bay, Oʻahu, Hawaiʻi and in the adjacent offshore. Instruments used were a YSI 6,600 sonde, a Turner 10AU fluorometer, an EPICS ALTRA flow cytometer, and an Illumina MiSeq v2 platform.\n\nThese data characterize the partitioning of microbial communities across sharp physiochemical gradients in surface seawaters connecting nearshore and offshore waters in the tropical Pacific. This study provides evidence for the ecological differentiation of SAR11 marine bacteria across nearshore to offshore waters in the tropical Pacific and further increases our understanding of how SAR11 genetic diversity partitions into distinct ecological units. Data were collected by Sarah J. Tucker, Kelle C. Freel, Elizabeth A. Monaghan, Clarisse E. S. Sullivan, Oscar Ramfelt, Yoshimi M. Rii, and Michael S. Rappé.\n\ncdm_data_type = Other\nVARIABLES:\nSample_ID (unitless)\ncollection_date (unitless)\ndepth (m)\nenv_broad_scale (unitless)\nenv_local_scale (unitless)\nenv_medium (unitless)\ngeo_loc_name (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\nSite_name (unitless)\nchlorophyll_a_ug_per_L (micrograms per Liter)\nph (no unit)\nsalinity (ppt)\n... (21 more variables)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_930084_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_930084_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_930084_v1/index.htmlTable https://www.bco-dmo.org/dataset/930084 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_930084_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_930084_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_930084_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_930163_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_930163_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_930163_v1/ public [Kāneʻohe Bay Time-series - phytoplankton and biogeochemistry] - Biological oceanographic measurements, 16S rRNA gene amplicons and metagenomes from surface seawater taken from August 2017 to June 2021 at sites within and adjacent to Kāneʻohe Bay, Oʻahu, Hawaiʻi (Population genomics and ecotypic divergence in the most dominant lineage of marine bacteria) These data include temperature, pH, salinity, chlorophyll a concentrations, cellular abundances of Prochlorococcus, Synechococcus, photosynthetic picoeukaryotes, and heterotrophic bacteria,16S ribosomal RNA gene amplicon libraries, metagenomes, inorganic nutrient concentrations, and photosynthetic pigment measurements via high performance liquid chromatography from surface seawater samples collected as part of the Kāneʻohe Bay Time-series (KByT). This dataset reflects near-monthly sampling of surface seawater that was conducted between between August 2017 and June 2021 at 10-12 sites within and adjacent to Kāneʻohe Bay, Oʻahu, Hawaiʻi. Instruments used were a YSI 6,600 sonde, a ProDSS multi-parameter sonde, a Turner 10AU fluorometer, a Beckman Coulter CytoFLEX S flow cytometer, a Seal Analytical AA3 HR Nutrient Autoanalyzer, an Illumina MiSeq v2 platform, and the Illumina NovaSeq 6000.\n\nThese data reveal a remarkably persistent transition in surface ocean biogeochemistry, phytoplankton biomass, and phytoplankton community structure, despite high water exchange and define surface ocean biogeochemical and phytoplankton regimes over space and time across nearshore to offshore waters in the tropical Pacific. These results provide insight into drivers of seasonal and spatial variability of phytoplankton communities. Data were collected and analyzed by Sarah J. Tucker, Yoshimi M. Rii, Kelle C. Freel, Keliʻiahonui Kotubetey, A. Hiʻilei Kawelo, and Kawika B. Winter, Michael S. Rappé.\n\ncdm_data_type = Other\nVARIABLES:\nSampleID (unitless)\nUniversal_Sample_ID (unitless)\nMetagenome (unitless)\nMetagenome_ID (unitless)\nYear (unitless)\nSampling_Order (unitless)\nSeason (unitless)\nMonth (unitless)\nMonth_abb (unitless)\nTime (hh:mm)\n... (68 more variables)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_930163_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_930163_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_930163_v1/index.htmlTable https://www.bco-dmo.org/dataset/930163 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_930163_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_930163_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_930163_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_700961.subset https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_700961 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_700961.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_700961/ public [Metabarcoding zooplankton at station ALOHA: NCBI SRA accession numbers] - NCBI Sequence Read Archive (SRA) accession numbers for fastq sequence files for each zooplankton community sample (Plankton Population Genetics project) (Basin-scale genetics of marine zooplankton) These data include sample information and accession links to sequence data at\nThe National Center for Biotechnology Information (NCBI) Sequence Read Archive\n(SRA).\n \nThis data submission consists of metabarcoding data for the zooplankton\ncommunity in the epipelagic, mesopelagic and upper bathypelagic zones\n(0-1500m) of the North Pacific Subtropical Gyre. The goal of this study was to\nassess the hidden diversity present in zooplankton assemblages in midwaters,\nand detect vertical gradients in species richness, depth distributions, and\ncommunity composition of the full zooplankton assemblage. Samples were\ncollected in June 2014 from Station ALOHA (22.75, -158) using a 1 meter square\nMultiple Opening and Closing Nets and Environmental Sampling System (MOCNESS,\n200um mesh), on R/V Falkor cruise FK140613. \\u00a0Next generation sequence\ndata (Illumina MiSeq, V3 chemistry, 300-bp paired-end) of the zooplankton\nassemblage derive from amplicons of the V1-V2 region of 18S rRNA (primers\ndescribed in Fonseca et al. 2010). The data includes sequences and read count\nabundance information for molecular OTUs from both holoplanktonic and\nmeroplanktonic taxa\n \nRelated dataset containing OTU tables and fasta sequences (representative /\nmost abundance read for each OTU):  \n[Metabarcoding zooplankton at ](\\\\http://www.bco-\ndmo.org/dataset/700279\\\\)[station](\\\\http://www.bco-\ndmo.org/dataset/700279\\\\)[ ALOHA: OTU tables and ](\\\\http://www.bco-\ndmo.org/dataset/700279\\\\)[fasta](\\\\http://www.bco-\ndmo.org/dataset/700279\\\\)[ files](\\\\http://www.bco-\ndmo.org/dataset/700279\\\\)\n\ncdm_data_type = Other\nVARIABLES:\nanalysis_name (unitless)\n... (15 more variables)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_700961_fgdc.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_700961/index.htmlTable https://www.bco-dmo.org/dataset/700961 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_700961.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_700961&showErrors=false&email= BCO-DMO bcodmo_dataset_700961
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_949666_v1 https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_949666_v1/ public [Metadata for longread sequencing of Carcinus maenas] - Metadata for longread sequencing of Carcinus maenas collected from Buzzard's Bay, Massachusetts from May 2022 to Aug 2022 (Collaborative Research: Tracking fine-scale selection to temperature at the invasion front of a highly dispersive marine predator) This project explores genomic changes in the invasive European green crab (Carcinus maenas), including at a putative inversion polymorphism. To begin to explore structural variation without a reference genome, we conducted semi-targeted longread sequencing of the C. maenas genome using MinION sequencing. This dataset includes individual metadata for 6 raw MinION reads, archived at GenBank's SRA under BioProject PRJNA1171011. This sequencing was conducted using crabs from Massachusetts waters.\n\ncdm_data_type = Other\nVARIABLES:\nsample_name (unitless)\nCollection_date (unitless)\nSRA_accession (unitless)\nbiosample_accession (unitless)\nembayment (unitless)\nstate (unitless)\nsex (unitless)\nlife_stage (unitless)\nrun_ID (unitless)\nsequencing_date (unitless)\nrun_day (days)\nprobe_set (unitless)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_949666_v1/index.htmlTable https://www.bco-dmo.org/dataset/949666 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_949666_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_949666_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_949666_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_949682_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_949682_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_949682_v1/ public [Metadata for RNASeq of green crabs in the northeast Pacific] - Metadata for RNASeq of green crabs collected in the northeast Pacific from Apr 2017 to Aug 2021 (Collaborative Research: Tracking fine-scale selection to temperature at the invasion front of a highly dispersive marine predator) This project explores genomic changes in the invasive European green crab (Carcinus maenas) in the northeast Pacific. It tracks the earliest stages of green crab invasion into a new environment where the species is predicted to have substantial ecological and economic impacts. Samples span time and space across the species range in the northeast Pacific, with a focus on areas where the species is currently expanding. This dataset includes individual metadata for 127 raw RNA-Seq reads, archived at GenBank's SRA under BioProject PRJNA1170986.\n\ncdm_data_type = Other\nVARIABLES:\nsample_name (unitless)\nSRA_accession (unitless)\nbiosample_accession (unitless)\nembayment (unitless)\nsite (unitless)\nstate (unitless)\nsex (unitless)\nsize_CW (Millimeters (mm))\ncolor (unitless)\ncollection_date (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\ncollector (unitless)\ncollector_affiliation (unitless)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_949682_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_949682_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_949682_v1/index.htmlTable https://www.bco-dmo.org/dataset/949682 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_949682_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_949682_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_949682_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_949610_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_949610_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_949610_v1/ public [Metadata for targeted genotyping of green crabs in the northeast Pacific] - Metadata for targeted genotyping of green crabs collected in the northeast Pacific from Aug 2016 to Oct 2022 (Collaborative Research: Tracking fine-scale selection to temperature at the invasion front of a highly dispersive marine predator) This project explores genetic changes in the invasive European green crab (Carcinus maenas) in the northeast Pacific, using targeted genotyping of SNPs identified in prior high-resolution population genomics in the region. It tracks the early stages of green crab invasion into a new environment, particularly through repeated sampling of multiple size-classes of crabs from the same sites over multiple years. Samples span time and space across the species range in the northeast Pacific, with a focus on the coast of Washington and Oregon. This dataset includes individual collection and accession metadata for 1,662 crabs, for which raw demultiplexed reads are archived at GenBank's SRA under BioProject PRJNA1177418.\n\ncdm_data_type = Other\nVARIABLES:\nsample_name (unitless)\nSRA_accession (unitless)\nbiosample_accession (unitless)\nembayment (unitless)\nsite (unitless)\nstate (unitless)\nsex (unitless)\nsize_CW (Millimeters (mm))\ncolor (unitless)\ncollection_date (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\ncollector (unitless)\ncollector_affiliation (unitless)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_949610_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_949610_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_949610_v1/index.htmlTable https://www.bco-dmo.org/dataset/949610 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_949610_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_949610_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_949610_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_745518.subset https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_745518 https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_745518/ public [Microbial eukaryotic focused metatranscriptome data] - Microbial eukaryotic focused metatranscriptome data from seawater collected in coastal California in May of 2015 (Protistan, prokaryotic, and viral processes at the San Pedro Ocean Time-series) Seawater was collected via Niskin bottles mounted with a CTD from the San Pedro Ocean Time-series (SPOT) station off the coast of Southern California near the surface (5 m), 150 and 890 m, in late May 2015. Raw sequence data was generated as part of a metatranscriptome study targeting the protistan community.  Raw sequences are available at the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) database (SRA Study ID: SRP110974, BioProject: PRJNA391503).  Sequences for BioProject PRJNA608423 will be available at NCBI on Jan 1st, 2021.\\r\\n\\r\\nThese data were published in Hu et al. (2018).\n\ncdm_data_type = Other\nVARIABLES:\nSRA_run (unitless)\nSRA_run_link (unitless)\nSRA_study (unitless)\nbioproject_accession (unitless)\nbiosample_accession (unitless)\nlibrary_ID (unitless)\ntitle (unitless)\nsample_name (unitless)\nlibrary_strategy (unitless)\nlibrary_source (unitless)\nlibrary_selection (unitless)\nlibrary_layout (unitless)\nplatform (unitless)\ninstrument_model (unitless)\ndesign_description (unitless)\nfiletype (unitless)\nfilename (unitless)\nfilename2 (unitless)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_745518/index.htmlTable https://www.bco-dmo.org/dataset/745518 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_745518.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_745518&showErrors=false&email= BCO-DMO bcodmo_dataset_745518
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_906740_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_906740_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_906740_v1/ public [Microbiome dynamics of coral reef and cleanerfish] - Microbiome dynamics of coral reef and cleanerfish from ecological surveys, in situ manipulations, and laboratory experiments conducted from 2020-2021 (Collaborative Research: Cleaning stations as hubs for the maintenance and recovery of microbial diversity on coral reefs.) Coral reefs host some of the most iconic symbiotic interactions in nature and are host to the highest diversity of life on the planet. Cleaning symbiosis, wherein small fish or shrimp remove external parasites and associated microorganisms from specific clients, is common on coral reefs. Sites on the reef occupied by cleaners, or \"cleaning stations\", attract a wide variety of fish species that engage in direct physical contact with the cleaner. In this study, we used a combination of ecological surveys, in situ manipulations, and laboratory experiments to examine the unique features of cleaning stations to understand transfer of bacterial and archaeal symbionts amongst fish and within coral reef environment. We used microbial 16S rRNA gene amplicons, environmental parameters, and other molecular tools to evaluate the dynamics between coral microbiomes, cleanerfish skin microbiomes, and the environment. This dataset contains metadata describing sequenced samples, including sample name, data deposition accession records, and measurements at the time of sample collection.\n\ncdm_data_type = Other\nVARIABLES:\nBioProject_accession (unitless)\nBioSample_accession (unitless)\nsample_name (unitless)\nSRA_accession (unitless)\ncollection_date (unitless)\ngeo_loc_name (unitless)\nhost (unitless)\nlat (degrees_north)\nlongitude (degrees_east)\nisolation_source (unitless)\nhost_common_name (unitless)\nhost_disease (unitless)\nhost_condition (unitless)\nhost_coral_cleaner_goby_pretreatment (unitless)\nhost_coral_reef_id (unitless)\nlocation_survey_date (unitless)\n... (13 more variables)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_906740_v1/index.htmlTable https://www.bco-dmo.org/dataset/906740 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_906740_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_906740_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_906740_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_926299_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_926299_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_926299_v1/ public [Microorganisms associated with doliolids] - Eukaryotic and prokaryotic microbial taxa retained by wild-caught doliolids collected during bloom events at three different shelf locations in the northern California Current system in June 2019. (Collaborative Research: Comparative feeding by gelatinous grazers on microbial prey) Doliolids have a unique ability to impact the marine microbial community through bloom events and high filtration rates. Their predation on large eukaryotic microorganisms is established and evidence of predation on smaller prokaryotic microorganisms is beginning to emerge. We studied the retention of both eukaryotic and prokaryotic microbial taxa by wild-caught doliolids in the northern California Current system. Doliolids were collected during bloom events identified at three different shelf locations with variable upwelling intensity.\n\ncdm_data_type = Other\nVARIABLES:\nbioproject_accession (unitless)\nbiosample_accession (unitless)\nsample_name (unitless)\nsra_sample_accession (unitless)\nsample_accession_title (unitless)\norganism_name (unitless)\norganism_taxonomy_id (unitless)\norganism_taxonomy_name (unitless)\nkeyword (unitless)\nbiosample_package (unitless)\ncollection_date (unitless)\ndepth (m)\nenv_broad_scale (unitless)\nenv_local_scale (unitless)\nenv_medium (unitless)\ngeo_loc_name (unitless)\nlatitude (Sampling_lat, degrees_north)\nlongitude (Sampling_lon, degrees_east)\nsize_frac (unitless)\nhost (unitless)\nsource_material_id (unitless)\nstatus (unitless)\n... (5 more variables)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_926299_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_926299_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_926299_v1/index.htmlTable https://www.bco-dmo.org/dataset/926299 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_926299_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_926299_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_926299_v1
log in [Microorganisms associated with pyrosomes] - High-throughput sequencing of the 16S rRNA gene, microscopy, and flow cytometry of pyrosome-associated microorganisms compared to seawater sampled during a Pyrosoma atlanticum bloom in the Northern California Current System in July 2018. (Collaborative Research: Comparative feeding by gelatinous grazers on microbial prey) Pyrosomes are widely distributed pelagic tunicates that have the potential to reshape marine food webs when they bloom. However, their grazing preferences and interactions with the background microbial community are poorly understood. The diversity, relative abundance, and taxonomy of pyrosome-associated microorganisms were compared to seawater during a Pyrosoma atlanticum bloom in the Northern California Current System using high-throughput sequencing of the 16S rRNA gene, microscopy, and flow cytometry.\n\ncdm_data_type = Other\nVARIABLES:\nbioproject_accession (unitless)\nbiosample_accession (unitless)\nsample_name (unitless)\nsra_sample_accession (unitless)\nsample_accession_title (unitless)\norganism_name (unitless)\norganism_taxonomy_id (unitless)\norganism_taxonomy_name (unitless)\nkeywords (unitless)\nbiosample_package (unitless)\ncollection_date (unitless)\nenv_broad_scale (unitless)\nenv_local_scale (unitless)\nenv_medium (unitless)\ngeo_loc_name (unitless)\nhost (unitless)\nlatitude (Sampling_lat, degrees_north)\nlongitude (Sampling_lon, degrees_east)\ndepth (m)\nhost_length (centimeter (cm))\nsource_material_id (unitless)\nstatus (unitless)\n... (20 more variables)\n BCO-DMO bcodmo_dataset_926093_v1
log in [Northern California Current Microorganisms] - 16S rRNA gene of microorganisms sampled along the Newport Hydrographic (NH) and Trinidad Head (TR) lines, in OR and CA in 2018 and 2019 (Collaborative Research: Comparative feeding by gelatinous grazers on microbial prey) The Northern California Current ecosystem is a productive system which supports major fisheries. To determine how the microbial community responds to variable upwelling, we examined the 16S rRNA gene of microorganisms from two size fractions, 0.2-1.6µm and greater than 1.6µm along the Newport Hydrographic (NH) and Trinidad Head (TR) lines, in OR and CA.\n\ncdm_data_type = Other\nVARIABLES:\nbioproject_accession (unitless)\nbiosample_accession (unitless)\nmessage (unitless)\nsample_name (unitless)\norganism (unitless)\ncollection_date (unitless)\ndepth (m)\nenv_broad_scale (unitless)\nenv_local_scale (unitless)\nenv_medium (unitless)\ngeo_loc_name (unitless)\nlatitude (Sampling_lat, degrees_north)\nlongitude (Sampling_lon, degrees_east)\nsize_frac (unitless)\nsra_run_accession (unitless)\nsra_study_accession (unitless)\nobject_status (unitless)\nlibrary_ID (unitless)\ntitle (unitless)\nlibrary_strategy (unitless)\nlibrary_source (unitless)\nlibrary_selection (unitless)\nlibrary_layout (unitless)\nplatform (unitless)\n... (5 more variables)\n BCO-DMO bcodmo_dataset_926850_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_924886_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_924886_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_924886_v1/ public [RADseq data from Atlantic silversides used for linkage and QTL mapping] - RADseq data from Atlantic silversides used for linkage and QTL mapping. (Collaborative research: The genomic underpinnings of local adaptation despite gene flow along a coastal environmental cline) ddRADseq data from 568 Atlantic silversides (Menidia menidia) that are either F1 or F2 offspring to wild-caught parents from Georgia and New York used in a controlled breeding experiment. The data were used to build linkage maps for each of the separate populations and their inter-population cross, and to perform quantitative trait locus mapping.\n\ncdm_data_type = Other\nVARIABLES:\nbioproject_accession (units)\nbiosample_accession (units)\ntaxonomic_name (units)\nmother_f0_sampling_location (units)\nlat_mother (degrees_north)\nlon_mother (degrees_east)\nfather_f0_sampling_location (units)\nlat_father (degrees_north)\nlon_father (degrees_east)\nSRA_study_accession (units)\nSRA_experiment_accession (units)\nSRA_run_accession (units)\nlibrary_ID (units)\ntitle (units)\nlibrary_strategy (units)\nlibrary_source (units)\nlibrary_selection (units)\nlibrary_layout (units)\nplatform (units)\ninstrument_model (units)\ndesign_description (units)\nfiletype (units)\nsample_name (units)\nfilename (units)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_924886_v1/index.htmlTable https://www.bco-dmo.org/dataset/924886 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_924886_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_924886_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_924886_v1
log in [Salp and pteropod associated microorganisms] - Salp and pteropod associated microorganisms from the  Western Edge of the Gulf Stream sampled in September 2019. (Collaborative Research: Comparative feeding by gelatinous grazers on microbial prey) Microbial mortality impacts the structure of food webs, carbon flow, and the interactions that create dynamic patterns of abundance across gradients in space and time in diverse ecosystems. In the oceans, estimates of microbial mortality by viruses, protists, and small zooplankton do not account fully for observations of loss, suggesting the existence of underappreciated mortality sources. We examined how ubiquitous mucous mesh feeders (i.e. gelatinous zooplankton) could contribute to microbial mortality in the open ocean. We coupled capture of live animals by blue-water diving to sequence-based approaches to measure the enrichment and selectivity of feeding by two coexisting mucous grazer taxa (pteropods and salps) on numerically dominant marine prokaryotes. We show that mucous mesh grazers consume a variety of marine prokaryotes and select between coexisting lineages and similar cell sizes. We show that Prochlorococcus may evade filtration more than other cells and that planktonic archaea are consumed by macrozooplanktonic grazers. Discovery of these feeding relationships identifies a new source of mortality for Earth's dominant marine microbes and alters our understanding of how top-down processes shape microbial community and function.\n\ncdm_data_type = Other\nVARIABLES:\nbioproject_accession (unitless)\nbiosample_accession (unitless)\nmessage (unitless)\nsample_name (unitless)\nsample_title (unitless)\norganism (unitless)\ncollection_date (unitless)\ndepth_f (Depth, feet)\nenv_broad_scale (unitless)\nenv_local_scale (unitless)\nenv_medium (unitless)\ngeo_loc_name (unitless)\nlatitude (Sampling_lat, degrees_north)\nlongitude (Sampling_lon, degrees_east)\n... (15 more variables)\n BCO-DMO bcodmo_dataset_926841_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_675040 https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_675040/ public [Salp genome and transcriptome] - Antarctic salp genome and RNAseq transcriptome from ARSV Laurence M. Gould, Umitaka-Maru, R/V Polarstern LMG1110, UM-08-09, ANT-XXVII-2 in the Southern Ocean from 2009-2011 (Salp_Antarctic project) (Population ecology of Salpa thompsoni based on molecular indicators) This dataset reports Salpa thompsoni specimens used for\ngenomics/transcriptomics with their GenBank accession links.\n \nRelated Dataset: [Salp sample log](\\\\https://www.bco-\ndmo.org/dataset/672600\\\\)\n\ncdm_data_type = Other\nVARIABLES:\nspecimen (unitless)\ncruise_id (unitless)\nstation (unitless)\nlength (millimeters)\nSRA_accession (unitless)\nBioSample_accession (Bio Sample Accession, unitless)\nSRA_accession_link (unitless)\nBioSample_accession_link (Bio Sample Accession Link, unitless)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_675040/index.htmlTable https://www.bco-dmo.org/dataset/675040 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_675040.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_675040&showErrors=false&email= BCO-DMO bcodmo_dataset_675040
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_746654.subset https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_746654 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_746654.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_746654/ public [Trichodesmium AHL metatranscriptomes_AE1409] - Trichodesmium AHL amendment metatranscriptomic reads accessions and metadata (Dissolved Phosphorus Processing by Trichodesmium Consortia: Quantitative Partitioning, Role of Microbial Coordination, and Impact on Nitrogen Fixation) Trichodesmium is a marine, diazotrophic cyanobacterium that plays a central role in the biogeochemical cycling of carbon and nitrogen. Colonies ubiquitously co-occur with a diverse microbiome of heterotrophic bacteria. Here we show that manipulation of the microbiome with quorum sensing acyl homoserine lactone (AHL) molecules significantly modulated rates of N2 fixation by Trichodesmium collected from the western North Atlantic, with both positive and negative effects of varied magnitude. Changes in Trichodesmium N2 fixation did not clearly correlate with changes in microbiome composition or geochemical patterns. Metatranscriptome sequencing revealed significant changes in the relative abundance of microbiome transcripts encoding metabolic functions consistent with quorum sensing responses in model bacteria. There was little overlap in specific microbiome transcriptional responses to AHL addition between stations, and this variability in microbiome gene expression may underpin the heterogeneous changes in Trichodesmium N2 fixation. These data suggest the microbiome could play a large and previously overlooked role in modulating Trichodesmium N2 fixation. This metadata form describes the metatranscriptomic sequencing reads that were used in the study.\n\ncdm_data_type = Other\nVARIABLES:\nbioproject_accession (unitless)\nbiosample_accession (unitless)\nlibrary_ID (unitless)\ntitle (unitless)\nlibrary_strategy (unitless)\nlibrary_source (unitless)\nlibrary_selection (unitless)\nlibrary_layout (unitless)\nplatform (unitless)\ninstrument_model (unitless)\ndesign_description (unitless)\nfiletype (unitless)\nassembly (unitless)\nfilename (unitless)\n... (7 more variables)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_746654/index.htmlTable https://www.bco-dmo.org/dataset/746654 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_746654.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_746654&showErrors=false&email= BCO-DMO bcodmo_dataset_746654

 
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