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log in [Cascadia Margin seep DOC and associated geochemical data] - Porewater dissolved organic carbon and associated geochemical data for methane seeps in the Cascadia Margin: Astoria Canyon, Barkley Canyon, Hydrate Ridge, and Bullseye Vent (Collaborative Research: Investigating the source and flux of dissolved organic carbon released from methane seeps to the deep-ocean) These data include vertical profiles of concentrations and stable carbon isotope values of dissolved organic carbon (DOC), dissolved inorganic carbon (DIC), total organic carbon (TOC) from sediment cores collected at methane seeps and reference sites. Sediment porosity and dissolved sulfate and methane concentrations are also presented. Methane seeps in Astoria Canyon, Barkley Canyon, Hydrate Ridge, and Bullseye Vent, along the Cascadia Margin, were sampled. These data were collected over two decades and across four cruises: PGC02-08 (CCGS John P. Tully, 2002), Hydrates 2004 Sea Trial (CCGS John P. Tully, 2004), AT50-14 (R/V Atlantis, 2023),  and AT50-29B (R/V Atlantis, 2024).\n\nThese data are used to assess the prevalence of methane-derived DOC at methane seeps, which may contribute ‘old' carbon to the deep ocean or serve as a source of reduced carbon for the deep ocean microbial loop.\n\ncdm_data_type = Other\nVARIABLES:\nRegion (unitless)\nCruise (unitless)\nSamplingDate (seconds since 1970-01-01T00:00:00Z)\nCoreID (unitless)\nSampleID (unitless)\nCoreType (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\nWaterDepth_m (meters (m))\nMidDepth_cmbsf (centimeters below sea floor (cmbsf))\nporosity (unitless)\nSO4_mM (millimolar (mM))\nCH4_uM (micromolar (uM))\nDOC_uM (micromolar (uM))\nDOC_d13C_permil (per mille (‰))\nDIC_mM (millimolar (mM))\n... (4 more variables)\n BCO-DMO bcodmo_dataset_959765_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_628993.subset https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_628993 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_628993.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_628993/ public [Incubation in diffuse flow vent fluids - Crab Spa] - Results from shipboard high-pressure incubations of diffuse flow vent fluids collected from the Crab Spa and Alvinella sites at East Pacific Rise during the AT26-10 expedition, Jan. 2014 (Microbial Communities at Deep-Sea Vents project) (An Integrated Study of Energy Metabolism, Carbon Fixation, and Colonization Mechanisms in Chemosynthetic Microbial Communities at Deep-Sea Vents) This dataset includes results from shipboard high-pressure incubations of\ndiffuse flow vent fluids collected from the Crab Spa (9.8398\\u00ba N,\n104.2913\\u00ba W) and Alvinella (9.8398\\u00ba N, 104.2915\\u00ba W) sites at\nEast Pacific Rise during the AT26-10 oceanographic expedition in January 2014.\nReported parameters include dates and time elapsed, flow rate, temperature,\npressure, and pH, and concentrations of NO3, NH4, H2, H2S, CH4.\n \nVent fluids used in shipboard incubations were corrected from diffuse flow\nvent sites at the East Pacific Rise (2503 m): Crab Spa (9.8398\\u00ba N,\n104.2913\\u00ba W) and Alvinella (9.8398\\u00ba N, 104.2915\\u00ba W) (see\ndescription in McNichol et al. [2016]). Fluids were collected using isobaric\ngas-tight samplers [Seewald et al., 2002] prior to their transfer to the\nshipboard continuous culture system [Foustoukos and Perez-Rodriguez, 2015].\nHere, high-pressure incubations (250 bars) were conducted at mesophilic (30\n\\u00baC) and thermophilic (50 \\u00baC) conditions to constrain the function\nand metabolic rates of denitrifying and DNRA microbial communities residing at\nCrab Spa and Alvinella, respectively. To enhance the activity of nitrate-\nrespiring anaerobic bacteria, an NO3- (5 mm) and H2(aq) (1.30 mM)-enriched\nmedium was introduced in the high-pressure incubations under strictly\nanaerobic conditions. Dissolved HCO3- (7.3 mm, 13C labeled) was used as added\ncarbon source. Vent fluids were introduced at a flow rate of 0.042 mL/min,\nwhile growth medium was added at a rate of 0.0042 mL/min. The two sets of\nexperiments were performed for 356 (Crab Spa) and 50 hours (Alvinella). Direct\ncell counts were conducted by staining cells with 0.1% acridine orange and\ncounting them with a fluorescence microscope. 15N/14N isotopic analysis of the\nNO3-, NH4+ and biomass were conducted with a Thermo Scientific Delta VPlus\nmass spectrometer and CE Instruments NA 2500 series elemental analyzer (EA).\n \nReferences:\n \nFoustoukos, D., and I. Perez-Rodriguez (2015), A continuous culture system for\nassessing microbial activities in the piezosphere, Applied and Environmental\nMicrobiology, 81(19), 6850-6856.\n \nMcNichol, J., S. P. Sylva, F. Thomas, C. D. Taylor, S. M. Sievert, and J. S.\nSeewald (2016), Assessing microbial processes in deep-sea hydrothermal systems\nby incubation at in situ temperature and pressure, Deep Sea Research Part I:\nOceanographic Research Papers, 115, 221-232.\n \nSeewald, J. S., K. W. Doherty, T. R. Hammar, and S. P. Liberatore (2002), A\nnew gas-tight isobaric sampler for hydrothermal fluids, Deep-Sea Research,\nPart I: Oceanographic Research Papers, 49(1), 189-196.\n\ncdm_data_type = Other\nVARIABLES:\ndescription (unitless)\n... (15 more variables)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_628993/index.htmlTable https://www.bco-dmo.org/dataset/628993 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_628993.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_628993&showErrors=false&email= BCO-DMO bcodmo_dataset_628993

 
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