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log in [Acropora cervicornis 16S rRNA sequence metadata: nutrient- and disease-exposed] - Acropora cervicornis 16S rRNA sequence metadata: nutrient- and disease-exposed from samples collected at  Mote Marine Laboratory in situ nursery from June to July 2022 (Collaborative Research: Tracking the interacting roles of the environment, host genotype, and a novel Rickettsiales in coral disease susceptibility) In the summer of 2022, 20 ramets each of the Acropora cervicornis genotypes ML-AC-36 and ML-AC-46 were collected from Mote's in situ coral nursery and subjected to three weeks of elevated nitrate, ammonium, and phosphate in the form of a slow-release fertilizer. The duration and concentration of this exposure were selected based on prior experiments conducted at Mote Marine Laboratory, which were found to alter microbial community profiles and growth rates in A. cervicornis (Klinges et al., 2022, 2023). During experiment, corals were held in 2-gallon aquaria with 5 corals per aquarium. As Mote Marine Laboratory's experimental aquarium system is plumbed into nearshore coastal water and thus has a higher nutrient load than the reef, an additional subset of 18 ramets of each genotype was collected from Mote's in situ coral nursery immediately prior to disease challenge to evaluate the impact of nearshore water on disease response. All ramets in the disease group were subjected to disease challenge in the form of a tissue homogenate produced from diseased fragments of random genotypes of A. cervicornis. Ramets in a comparative unexposed group were exposed to a homogenate produced from healthy fragments of random genotypes of A. cervicornis.  We collected 190 samples for 16S rRNA sequencing, including prior to nutrient exposure, nutrient-exposed and -unexposed corals prior to disease challenge, and at several timepoints throughout disease exposure capturing both diseased and apparently healthy phenotypes. We additionally collected numerous samples for measurements of immune-related proteins. In brief, we found that disease challenge significantly impacted microbial communities, leading to significant differences in community composition between disease-exposed samples that developed disease, exposed samples that resisted disease, and healthy unexposed controls. The taxa Algicola, Halarcobacter, Saprospira, Shimia, Tenacibaculum, Thalassolituus, and Thalassotalea increased in abundance in samples that developed disease. Of the genotypes chosen for the experiment, one genotype was dominated by the putative parasite Aquarickettsia. We found that both genotypes were susceptible to disease, in contrast to previous results indicating that genotypes with low abundance of Aquarickettsia and relatively diverse microbiomes were disease-resistant. Raw sequence reads have been submitted to SRA under PRJNA1024453 with an embargo until publication.\n\ncdm_data_type = Other\nVARIABLES:\nNCBI_Project (unitless)\nsample_name (unitless)\n... (30 more variables)\n BCO-DMO bcodmo_dataset_924465_v1
log in [Acropora cervicornis protein measurements: nutrient- and disease-exposed] - Acropora cervicornis protein measurements: nutrient- and disease-exposed from samples collected at  Mote Marine Laboratory in situ nursery from June to July 2022 (Collaborative Research: Tracking the interacting roles of the environment, host genotype, and a novel Rickettsiales in coral disease susceptibility) In the summer of 2022, 20 ramets each of the Acropora cervicornis genotypes ML-AC-36 and ML-AC-46 were collected from Mote's in situ coral nursery and subjected to three weeks of elevated nitrate, ammonium, and phosphate in the form of a slow-release fertilizer. The duration and concentration of this exposure were selected based on prior experiments conducted at Mote Marine Laboratory, which were found to alter microbial community profiles and growth rates in A. cervicornis (Klinges et al., 2022, 2023). During experiment, corals were held in 2-gallon aquaria with 5 corals per aquarium. As Mote Marine Laboratory's experimental aquarium system is plumbed into nearshore coastal water and thus has a higher nutrient load than the reef, an additional subset of 18 ramets of each genotype was collected from Mote's in situ coral nursery immediately prior to disease challenge to evaluate the impact of nearshore water on disease response. All ramets in the disease group were subjected to disease challenge in the form of a tissue homogenate produced from diseased fragments of random genotypes of A. cervicornis. Ramets in a comparative unexposed group were exposed to a homogenate produced from healthy fragments of random genotypes of A. cervicornis.  To evaluate the effect of Aquarickettsia infection; nutrient enrichment; disease exposure; and the combination of these factors on coral immune function, a number of immune related proteins and antioxidants were measured using microplate assays from a total of 55 samples taken prior to disease exposure and following disease exposure as corals either resisted or developed disease. Total host protein and the antioxidant superoxide dismutase were found to be higher in healthy corals regardless of genotype or nutrient enrichment. Phenoloxidase and prophenoloxidase were higher in concentration in diseased samples compared to healthy samples. Disease-exposed but apparently healthy corals had higher superoxide dismutase, prophenoloxidase, and peroxidase activity than either healthy unexposed corals or diseased corals despite total protein concentrations that were lower than healthy unexposed corals.\n\ncdm_data_type = Other\nVARIABLES:\nFull_ID (unitless)\nDate (unitless)\nTP (mg)\nPOX (ΔAbs470nm)\nNormalized_POX (ΔAbs470nm/mg protein)\n... (17 more variables)\n BCO-DMO bcodmo_dataset_924466_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_706075 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_706075.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_706075/ public [Calcification Rates] - Calcification rates of Porites corals collected from a naturally high-Ωar reef and a naturally low-Ωar reef in Palau incubated at three experimental Ωar conditions (Toward Predicting the Impact of Ocean Acidification on Net Calcification by a Broad Range of Coral Reef Ecosystems: Identifying Patterns and Underlying Causes) Calcification rates of Porites corals collected from a naturally high-\\u03a9ar reef and a naturally low-\\u03a9ar reef in Palau incubated at three experimental \\u03a9ar conditions.\n\ncdm_data_type = Other\nVARIABLES:\ncoral_id (unitless)\norigin_reef_omega_AR (unitless)\nCO2_treatment (unitless)\nexperiment_omega_AR (unitless)\ncalc_rate_buoyant_weight (milligrams per square centimeter per week (mg/cm2/wk))\ncalc_rate_alk_anomaly (milligrams per sqare centimeter per hour (mg/cm2/hr))\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_706075/index.htmlTable https://www.bco-dmo.org/dataset/706075 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_706075.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_706075&showErrors=false&email= BCO-DMO bcodmo_dataset_706075
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_752642 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_752642.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_752642/ public [Coral 3D scans] - Coral stereolithography files and sampling information for Acropora palmata, A. cervicornis, and A. prolifera from St. Thomas U.S. Virgin Islands and Belize, 2015 (Collaborative research: Is hybridization among threatened Caribbean coral species the key to their survival or the harbinger of their extinction?) Coral specimens collected in the St. Thomas, U.S. Virgin Islands and Belize were 3-dimensionally scanned. This dataset includes metadata on the coral samples such as the collection location and conditions, and the dimensions of the samples. The STL files are available for download in the Supplemental Documentation section below.\n\ncdm_data_type = Other\nVARIABLES:\nscan_filename (unitless)\nSpecies (unitless)\nCoral_ID (unitless)\nGenetics_ID (unitless)\nLocation (unitless)\nSite (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\ntime (Scan Date, seconds since 1970-01-01T00:00:00Z)\nCollection_Depth_ft (feet)\nCurrent_Direction_from (unitless)\nWave_Energy (unitless)\nBenthic_Type (unitless)\nLength_cm (centimeters)\nWidth_cm (centimeters)\nHeight_cm (centimeters)\nVolume (centimeters^3)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_752642_fgdc.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_752642/index.htmlTable https://www.bco-dmo.org/dataset/752642 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_752642.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_752642&showErrors=false&email= BCO-DMO bcodmo_dataset_752642
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_707106 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_707106.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_707106/ public [Coral calcification histories] - Annual calcification histories for corals from ten Palau reef sites representing lagoon and barrier reef environments (Constraining Thermal Thresholds and Projections of Temperature Stress on Pacific Coral Reefs Over the 21st Century: Method Refinement and Application) Annual calcification histories for corals from ten Palau reef sites representing lagoon and barrier reef environments.\n\ncdm_data_type = Other\nVARIABLES:\nsite (unitless)\nreef_type (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\ncoral_id (unitless)\nyear (unitless)\ndensity (grams per square centimeter (g/cm2))\nextension (centimeters per year (cm/yr))\ncalcification (grams per square centimeter per year (g/cm2/yr))\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_707106_fgdc.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_707106/index.htmlTable https://www.bco-dmo.org/dataset/707106 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_707106.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_707106&showErrors=false&email= BCO-DMO bcodmo_dataset_707106
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_709415 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_709415.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_709415/ public [Core stress bands] - Stress bands in coral cores for corals from Palau reefs (Constraining Thermal Thresholds and Projections of Temperature Stress on Pacific Coral Reefs Over the 21st Century: Method Refinement and Application) Stress bands in coral cores for corals from Palau reefs.\n\ncdm_data_type = Other\nVARIABLES:\nreef_type (unitless)\nsite (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\ncoral_id (unitless)\ndate_collected (unitless)\ndate_range (unitless)\nstress_band_1998 (unitless)\nstress_band_2010 (unitless)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_709415_fgdc.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_709415/index.htmlTable https://www.bco-dmo.org/dataset/709415 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_709415.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_709415&showErrors=false&email= BCO-DMO bcodmo_dataset_709415
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_776448 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_776448.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_776448/ public [Linear Extension] - Upward vertical growth of P. damicornis measured from a dye line, 2018-2019 (Collaborative Research: Climate Change, Mesoscale Oceanography, and the Dynamics of Eastern Pacific Coral Reefs) Upward vertical growth of P. damicornis measured from a dye line, 2018-2019.\n\ncdm_data_type = Other\nVARIABLES:\nGulf (unitless)\nSite (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\nTime_Period (Years)\nCoral_ID (unitless)\nBranch (unitless)\nSubbranch (unitless)\nOutside (millimeters)\nInside (millimeters)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_776448_fgdc.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_776448/index.htmlTable https://www.bco-dmo.org/dataset/776448 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_776448.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_776448&showErrors=false&email= BCO-DMO bcodmo_dataset_776448
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_688125 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_688125.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_688125/ public [Palau dissepiment counts] - Dissepiment counts obtained from staining experiment of Porites coral from Palau (Can Coral Reefs in the Central Pacific Survive Ocean Warming? A 2015 El Nino Test) Dissepiment counts obtained from staining experiment of Porites coral from Palau.\n\ncdm_data_type = Other\nVARIABLES:\ncoral_id (unitless)\nsite (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\ndensity (grams per cubic centimeter (g cm-3))\nextension (centimeters per year (cm yr-1))\ncalcification (grams per square centimeter per year (g cm-2 yr-1))\ntissue_layer (millimeters (mm))\ndissepiments_11_2013 (unitless (counts))\ndissepiments_01_2015 (unitless (counts))\ndissepiments_formed (unitless (counts))\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_688125_fgdc.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_688125/index.htmlTable https://www.bco-dmo.org/dataset/688125 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_688125.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_688125&showErrors=false&email= BCO-DMO bcodmo_dataset_688125
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_904962_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_904962_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_904962_v1/ public [Physiology of Montipora capitata and Porites compressa in response to marine heatwaves] - Physiology of Montipora capitata and Porites compressa from October 2019 to September 2022 in response to marine heatwaves (2015 and 2019) in Hawaiʻi (RAPID: Collaborative Research: Disentangling the effects of heat stress versus bleaching phenotype on coral performance) Increasingly frequent marine heatwaves are devastating coral reefs. Corals that survive these extreme events must rapidly recover if they are to withstand subsequent events, and long-term survival in the face of rising ocean temperatures may hinge on recovery capacity and acclimatory gains in heat tolerance over an individual's lifespan. To better understand coral recovery trajectories in the face of successive marine heatwaves, we monitored the responses of bleaching-susceptible and bleaching-resistant individuals of two dominant coral species in Hawaiʻi, Montipora capitata and Porites compressa, over a decade that included three marine heatwaves. This dataset includes all of the physiological data for both species from October 2019 to September 2022.\n\ncdm_data_type = Other\nVARIABLES:\nCoral_ID (unitless)\nSpecies (unitless)\nBleach (unitless)\nDate (unitless)\nPeriod (unitless)\nMonths (unitless)\nProtein (micrograms per centimeter squared  (mg cm-2))\nSym_density (10^-6 cells per centimeter squared (10-6 cells cm-2))\nChl (micrograms per centimeter squared (ug cm-2))\nCaCO3 (grams ler milliliter (g mL-1))\ninternal_volume_percent (percent (%))\nTAC_CRE (uM copper reducing equivalents (CRE) mg protein-1)\nug_Chl_a_per_zoox (micrograms per cell (ug cell-1))\npg_Chl_a_per_zoox (picograms per cell (ug cell-1))\nCitrate_synthase (citrate synthase mg protein-1)\nAFDW (milligrams per centimeter squared (mg cm-2))\nEndolithic (unitless)\nPPO (change in absorbance minute-1 mg protein-1)\nMelanin_per_tissue (unitless)\n... (4 more variables)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_904962_v1/index.htmlTable https://www.bco-dmo.org/dataset/904962 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_904962_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_904962_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_904962_v1
log in [Pocillopora species ID from Recharge project] - Differential effects of nutrients and consumer pressure on sympatric cryptic coral species (Pocillopora spp.) in Moorea, French Polynesia sampled in November 2021. (Collaborative research: Coral community resilience: testing the role of hidden diversity in pocilloporid corals at Moorea) Cryptic species (evolutionarily distinct lineages that do not align with morphologically defined species) are being increasingly discovered but are poorly integrated into ecological theory. In particular, we still lack a useful understanding of if and how cryptic species differ in ways that affect community recovery from disturbances and responses to anthropogenic stressors, such as the removal of consumers and pollution from nutrients. \n\nOn coral reefs, nutrient pollution increases the growth of macroalgae that displaces corals. Reductions in herbivorous fishes reduce the suppression of macroalgae, while reductions in coralivorous fishes reduce predation on corals. An unresolved question is if and how cryptic coral species respond differently to these impacts, thereby differing in their ability to influence coral community dynamics and maintain coral dominance. Therefore, we assessed how the response of cryptic Pocillopora species over a period of three years following a simulated disturbance from a cyclone depended on the experimental reduction of fish consumer pressure and nutrient addition. After three years, five morphologically cryptic, but genetically distinct, Pocillopora species recruited to the reef. However, recruitment was dominated by two species: P. tuahiniensis (46%) and P. meandrina (43%). \n\nUnder ambient conditions, recruitment of P. tuahiniensis and P. meandrina was similar, but experimentally reducing consumer pressure increased recruitment of P. tuahiniensis by up to 73% and reduced recruitment of P. meandrina by up to 49%. In both species, nutrient enrichment increased recruitment and colony growth rates equally, but colonies of P. tuahiniensis grew faster, and were up to 25% larger after three years, than those of P. meandrina, and growth was unaffected by reduced consumer pressure. Predation by excavating corallivorous fish was higher for P. meandrina than for P. tuahiniensis, especially under nutrient enrichment. In contrast, polyp extension (an indicator of elevated heterotrophic feeding as well as susceptibility and attractiveness to corallivores) was lower for P. meandrina than for P. tuahiniensis, especially under low to medium consumer pressure. Overall, we uncovered ecological differences in the response of morphologically cryptic foundation species to two pervasive stressors on coral reefs. \n\nOur results demonstrate how cryptic species respond differently to key anthropogenic stressors, which may contribute to response diversity that can support ecological resilience or increase extinction risk.\n\ncdm_data_type = Other\nVARIABLES:\nlatitude (degrees_north)\n... (14 more variables)\n BCO-DMO bcodmo_dataset_964151_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_705851 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_705851.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_705851/ public [Reciprocal transplants] - Data from reciprocal transplant experiments conducted on Porites coral collected on Palau in December 2012 (Toward Predicting the Impact of Ocean Acidification on Net Calcification by a Broad Range of Coral Reef Ecosystems: Identifying Patterns and Underlying Causes) Data from reciprocal transplant experiments conducted on Porites coral collected on Palau in December 2012.\n\ncdm_data_type = Other\nVARIABLES:\ncoral_id (unitless)\ntransplant_duration (months)\noriginal_omegaAR (Original Omega AR, unitless)\ntransplant_omegaAR (Transplant Omega AR, unitless)\ntreatment_code (unitless)\ncalc_rate (milligrams per square centimeter per month (mg/cm2/month))\nstatus (unitless)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_705851/index.htmlTable https://www.bco-dmo.org/dataset/705851 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_705851.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_705851&showErrors=false&email= BCO-DMO bcodmo_dataset_705851

 
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