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| griddap | Subset | tabledap | Make A Graph | wms | files | Accessible | Title | Summary | FGDC | ISO 19115 | Info | Background Info | RSS | Institution | Dataset ID | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_897359_v1 | https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_897359_v1/ | public | [Ruegeria pomeroyi DOP hydrolysis rates] - Dissolved organic phosphorus (DOP) hydrolysis rates from Ruegeria pomeroyi laboratory cultures (Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle) | Dissolved organic phosphorus (DOP) hydrolysis rates from marine bacterium Ruegeria pomeroyi laboratory cultures.\n\nThese data were collected as part of a study of \"Dissolved organic phosphorus utilization by the marine bacterium Ruegeria pomeroyi DSS-3 reveals chain length-dependent polyphosphate degradation\" (Adams et al., 2022).\n\nStudy abstract:\nDissolved organic phosphorus (DOP) is a critical nutritional resource for marine microbial communities. However, the relative bioavailability of different types of DOP, such as phosphomonoesters (P-O-C) and phosphoanhydrides (P-O-P), is poorly understood. Here we assess the utilization of these P sources by a representative bacterial copiotroph, Ruegeria pomeroyi DSS-3. All DOP sources supported equivalent growth by R. pomeroyi, and all DOP hydrolysis rates were upregulated under phosphorus depletion (-P). A long-chain polyphosphate (45polyP) showed the lowest hydrolysis rate of all DOP substrates tested, including tripolyphosphate (3polyP). Yet the upregulation of 45polyP hydrolysis under -P was greater than any other substrate analyzed. Proteomics revealed three common P acquisition enzymes potentially involved in polyphosphate utilization, including two alkaline phosphatases, PhoD and PhoX, and one 5'-nucleotidase (5'-NT). Results from DOP substrate competition experiments show that these enzymes likely have broad substrate specificities, including chain length-dependent reactivity toward polyphosphate. These results confirm that DOP, including polyP, are bioavailable nutritional P sources for R. pomeroyi, and possibly other marine heterotrophic bacteria. Furthermore, the chain-length dependent mechanisms, rates and regulation of polyP hydrolysis suggest that these processes may influence the composition of DOP and the overall recycling of nutrients within marine dissolved organic matter.\n\ncdm_data_type = Other\nVARIABLES:\nMedia_Type (unitless)\nGrowth_Phase (unitless)\nSample_Type (unitless)\nDOP_Substrate (unitless)\nHydrolysis_Rate (umol Pi L-1 hr-1)\n | https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_897359_v1/index.htmlTable | https://www.bco-dmo.org/dataset/897359
| https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_897359_v1.rss | https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_897359_v1&showErrors=false&email= | BCO-DMO | bcodmo_dataset_897359_v1 | ||||||
| https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_929459_v1 | https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_929459_v1.graph | https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_929459_v1/ | public | [Synechococcus DOP Displacement Experiment] - Laboratory-cultured Synechococcus (WH8102 and WH5701) MUF-P hydrolysis inhibition by dissolved organic phosphorus (DOP) from experiments between 2018-2023 (Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle) | The affinity of Synechococcus (WH8102 and WH5701) alkaline phosphatases for different DOP model substrates was examined in laboratory experiments through its ability to inhibit the hydrolysis of the fluorogenic substrate 4-methylumbelliferyl phosphate (MUF-P). These data were collected as part of a study of \"Dissolved organic phosphorus bond-class utilization by Synechococcus\" (Waggoner et al., 2024).\n \nStudy Abstract:\nDissolved organic phosphorus (DOP) contains compounds with phosphoester (P-O-C), phosphoanhydride (P-O-P), and phosphorus-carbon (P-C) bonds. Despite DOP's importance as a nutritional source for marine microorganisms, the bioavailability of each bond-class to the widespread cyanobacterium Synechococcus remains largely unknown. This study evaluates bond-class specific DOP utilization by cultures of an open ocean and a coastal ocean Synechococcus strain. Both strains exhibited comparable growth rates when provided phosphate, short-chain and long-chain polyphosphate (P-O-P), adenosine 5'-triphosphate (P-O-C and P-O-P), and glucose-6-phosphate (P-O-C) as the phosphorus source. However, growth rates on phosphomonoester adenosine 5'-monophosphate (P-O-C) and phosphodiester bis(4-methylumbelliferyl) phosphate (C-O-P-O-C) varied between strains, and neither strain grew on selected phosphonates. Consistent with the growth measurements, both strains preferentially hydrolyzed 3-polyphosphate, followed by adenosine 5'-triphosphate, and then adenosine 5'-monophosphate. The strains' exoproteome contained phosphorus hydrolases, which combined with enhanced cell-free hydrolysis of 3-polyphosphate and adenosine 5'-triphosphate under phosphate deficiency, suggests active mineralization of short-chain polyphosphate by Synechococcus' exoproteins. Synechococcus alkaline phosphatases presented broad substrate specificities, including activity towards short-chain polyphosphate, with varying affinities between the two strains. Collectively, these findings underscore the potentially significant role of compounds with phosphoanhydride bonds in Synechococcus phosphorus nutrition, thereby expanding our understanding of microbially-mediated DOP cycling in marine ecosystems.\n\ncdm_data_type = Other\nVARIABLES:\nsynechococcus_strain (unitless)\nMUF_P_conc (micromoles per liter (umol L-1))\n... (5 more variables)\n | https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_929459_v1/index.htmlTable | https://www.bco-dmo.org/dataset/929459
| https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_929459_v1.rss | https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_929459_v1&showErrors=false&email= | BCO-DMO | bcodmo_dataset_929459_v1 | |||||
| https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_928984_v1 | https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_928984_v1.graph | https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_928984_v1/ | public | [Synechococcus DOP Hydrolysis Experiment - hydrolysis rates] - Dissolved organic phosphorus (DOP) hydrolysis rates from marine cyanobacterium Synechococcus (WH8102 and WH5701) laboratory cultures from experiments between 2018-2023 (Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle) | Dissolved organic phosphorus (DOP) hydrolysis rates from marine cyanobacterium Synechococcus (WH8102 and WH5701) laboratory cultures. These data were collected as part of a study of \"Dissolved organic phosphorus bond-class utilization by Synechococcus\" (Waggoner et al., submitted).\n\n\n\nStudy Abstract:\nDissolved organic phosphorus (DOP) contains compounds with phosphoester (P-O-C), phosphoanhydride (P-O-P), and phosphorus-carbon (P-C) bonds. Despite DOP's importance as a nutritional source for marine microorganisms, the bioavailability of each bond-class to the widespread cyanobacterium Synechococcus remains largely unknown. This study evaluates bond-class specific DOP utilization by cultures of an open ocean and a coastal ocean Synechococcus strain. Both strains exhibited comparable growth rates when provided phosphate, short-chain and long-chain polyphosphate (P-O-P), adenosine 5'-triphosphate (P-O-C and P-O-P), and glucose-6-phosphate (P-O-C) as the phosphorus source. However, growth rates on phosphomonoester adenosine 5'-monophosphate (P-O-C) and phosphodiester bis(4-methylumbelliferyl) phosphate (C-O-P-O-C) varied between strains, and neither strain grew on selected phosphonates. Consistent with the growth measurements, both strains preferentially hydrolyzed 3-polyphosphate, followed by adenosine 5'-triphosphate, and then adenosine 5'-monophosphate. The strains' exoproteome contained phosphorus hydrolases, which combined with enhanced cell-free hydrolysis of 3-polyphosphate and adenosine 5'-triphosphate under phosphate deficiency, suggests active mineralization of short-chain polyphosphate by Synechococcus' exoproteins. Synechococcus alkaline phosphatases presented broad substrate specificities, including activity towards short-chain polyphosphate, with varying affinities between the two strains. Collectively, these findings underscore the potentially significant role of compounds with phosphoanhydride bonds in Synechococcus phosphorus nutrition, thereby expanding our understanding of microbially-mediated DOP cycling in marine ecosystems.\n\ncdm_data_type = Other\nVARIABLES:\nsynechococcus_strain (unitless)\n... (7 more variables)\n | https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_928984_v1/index.htmlTable | https://www.bco-dmo.org/dataset/928984
| https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_928984_v1.rss | https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_928984_v1&showErrors=false&email= | BCO-DMO | bcodmo_dataset_928984_v1 | |||||
| https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_929212_v1 | https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_929212_v1.graph | https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_929212_v1/ | public | [Synechococcus Growth on DOP Experiments - IVF] - Laboratory-cultured Synechococcus (WH8102 and WH5701) growth (vivo fluorescence) on dissolved organic phosphorus (DOP) from experiments between 2018-2023 (Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle) | Laboratory culture Synechococcus (WH8102 and WH5701) growth on dissolved organic phosphorus (DOP). These data were collected as part of a study of \"Dissolved organic Phosphorus bond-class utilization by Synechococcus\" (Waggoner et al. 2024). \n\nStudy Abstract:\nDissolved organic phosphorus (DOP) contains compounds with phosphoester (P-O-C), phosphoanhydride (P-O-P), and phosphorus-carbon (P-C) bonds. Despite DOP's importance as a nutritional source for marine microorganisms, the bioavailability of each bond-class to the widespread cyanobacterium Synechococcus remains largely unknown. This study evaluates bond-class specific DOP utilization by cultures of an open ocean and a coastal ocean Synechococcus strain. Both strains exhibited comparable growth rates when provided phosphate, short-chain and long-chain polyphosphate (P-O-P), adenosine 5'-triphosphate (P-O-C and P-O-P), and glucose-6-phosphate (P-O-C) as the phosphorus source. However, growth rates on phosphomonoester adenosine 5'-monophosphate (P-O-C) and phosphodiester bis(4-methylumbelliferyl) phosphate (C-O-P-O-C) varied between strains, and neither strain grew on selected phosphonates. Consistent with the growth measurements, both strains preferentially hydrolyzed 3-polyphosphate, followed by adenosine 5'-triphosphate, and then adenosine 5'-monophosphate. The strains' exoproteome contained phosphorus hydrolases, which combined with enhanced cell-free hydrolysis of 3-polyphosphate and adenosine 5'-triphosphate under phosphate deficiency, suggests active mineralization of short-chain polyphosphate by Synechococcus' exoproteins. Synechococcus alkaline phosphatases presented broad substrate specificities, including activity towards short-chain polyphosphate, with varying affinities between the two strains. Collectively, these findings underscore the potentially significant role of compounds with phosphoanhydride bonds in Synechococcus phosphorus nutrition, thereby expanding our understanding of microbially-mediated DOP cycling in marine ecosystems.\n\ncdm_data_type = Other\nVARIABLES:\nsynechococcus_strain (unitless)\nexperiment_number (unitless)\ntime_day (days)\nDOP_substrate (unitless)\nin_vivo_fluorescence_trip1 (relative fluorescence units (RFU))\nin_vivo_fluorescence_trip2 (relative fluorescence units (RFU))\nin_vivo_fluorescence_trip3 (relative fluorescence units (RFU))\n | https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_929212_v1/index.htmlTable | https://www.bco-dmo.org/dataset/929212
| https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_929212_v1.rss | https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_929212_v1&showErrors=false&email= | BCO-DMO | bcodmo_dataset_929212_v1 |