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log in [North Pacific Micromonas Culture Experiment] - Response of Micromonas sp. to iron-limitation sampled aboard R/V Roger Revelle RR1813 in the Subarctic North Pacific near Station  PAPA (50 N, 144.8 W) from August to September 2018 (Collaborative Research: Evaluating the contribution of small eukaryotes to nitrate-based new production in the North Pacific Subtropical Gyre) Small eukaryotic phytoplankton can account for considerable amounts of biomass and primary production in high nutrient, low chlorophyll (HNLC) regions of the ocean where iron-limitation is pronounced. However, the strategies these cells invoke to cope under low iron conditions and the extent to which they are responsible for new production (i.e., the fraction of primary production supported by nutrients from outside of the euphotic zone) is unclear. Here, we examine how a representative picoeukaryote, the chlorophyte Micromonas sp., recently isolated from the iron-limited subarctic North Pacific Ocean, responds to iron-limitation and quantify its potential contribution to new production. Iron-limited Micromonas exhibits reductions in cellular growth rate, volume, and carbon and nitrogen quotas along with a restructuring of cellular metabolism as inferred through gene expression. Gene expression and pathway analyses show evidence of cellular strategies to mitigate iron limitation but conservation of genes related to nitrogen uptake and utilization. Additionally, when grown on nitrate, cellular carbon and nitrogen quotas ranged from 0.02 – 0.17 pmol C cell-1 and 3.3 x 10-3 – 2.2 x 10-2 pmol N cell-1 as a function of iron status.\n\ncdm_data_type = Other\nVARIABLES:\nCruise (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\nTreatment (unitless)\nReplicate (unitless)\nVolume (liter (L))\nCell_Count (cell per liter (cell L-1))\nCarbon (grams (g))\nNitrogen (grams (g))\nCHL (micrograms per liter (µg L-1))\nFv_Fm (unitless)\nSigma (unitless)\nGrowth_Rates (per day (d-1))\n BCO-DMO bcodmo_dataset_945544_v1
log in [Palau Coral Reef Experiment 2018:  Pulse Amplitude Modulation (PAM) fluorometry] - Coral pulse amplitude modulation (PAM) fluorometry data from a heating experiment using samples collected from Nikko Bay and Rebotel Reef in Palau in the spring of 2018 (Collaborative Research: Stability, flexibility, and functionality of thermally tolerant coral symbioses) Coral reefs surrounding Palau, Micronesia are living within a broad range of thermal habitats. Specifically, corals living on the offshore barrier reefs surrounding Palau reside in waters with low temperature variability compared to the much warmer and more acidic waters of near shore environments surrounding the Rock Island habitats. \n\nThis study was designed to test the differences in thermal physiology among two species of reef corals that reside at both of these locations. Specifically, we examined how short-term elevated temperature influences  the photochemistry of each coral species from each location before and after heating, as measured by active chlorophyll fluorescence recorded by pulse amplitude modulation (PAM) fluorometry.\n\ncdm_data_type = Other\nVARIABLES:\nLocation_Name (unitless)\nlongitude (degrees_east)\nlatitude (degrees_north)\nSpecies (unitless)\nDate (unitless)\nDay (unitless)\nTreatment (unitless)\nLocation (unitless)\nColony (unitless)\nFrag (unitless)\nSymbiont (unitless)\nFq_Fm (unitless)\nFv_Fm (unitless)\nQm (unitless)\n BCO-DMO bcodmo_dataset_855054_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_732890 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_732890.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_732890/ public [Symbiodinium Fv/Fm over a temperature gradient] - Fv/Fm for cultured Clade A & B Symbiodinium with 2 treatments measured over a range of temperatures (Coevolution of scleractinian corals and their associated microorganisms) Coral photosynthetic endosymbionts (Symbiodinium) are phylogenetically very diverse, yet the extent of inter- and intraspecific functional variation within clades remains largely underexplored. Understanding this variability will be critical for future research on climate change mediated responses. A properly functioning thylakoid membrane is essential for optimal photosynthetic performance both in free-living and in hospite conditions. Here we analyze the thylakoid membrane melting points of 13  Symbiodinium strains from species in Clades B and A, grown at both control (26 \\u00b0C)  and high temperature (31 \\u00b0C). We observed a broad range of responses to thermal stress regardless of taxonomic rank. Our results support and augment a growing body of literature demonstrating that functional differences among Symbiodinium spp. are as distinct at lower taxonomic levels (i.e. interspecific) as they are among major clades. These findings highlight the importance of assessing the variability of plastid traits across the Symbiodinium tree.\n\ncdm_data_type = Other\nVARIABLES:\nStrain (unitless)\nSpecies (unitless)\nTreatment (unitless)\nReplicate (unitless)\nTemperature (degrees Celsius)\nFv_Fm (dimensionless)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_732890/index.htmlTable https://www.bco-dmo.org/dataset/732890 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_732890.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_732890&showErrors=false&email= BCO-DMO bcodmo_dataset_732890
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_907003_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_907003_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_907003_v1/ public [Tracer data] - Nutrient transfer experiments with host coral and symbionts under varying environmental conditions in March 2014 and March 2015 (Collaborative Research: Stability, flexibility, and functionality of thermally tolerant coral symbioses) Symbiotic mutualisms are essential to ecosystems and numerous species across the tree of life. For reef-building corals, the benefits of their association with endosymbiotic dinoflagellates differ within and across taxa, and nutrient exchange between these partners is influenced by environmental conditions. Furthermore, it is widely assumed that corals associated with symbionts in the genus Durusdinium tolerate high thermal stress at the expense of lower nutrient exchange to support coral growth. We traced both inorganic carbon (H13CO3–) and nitrate (15NO3–) uptake by divergent symbiont species and quantified nutrient transfer to the host coral under normal temperatures as well as in colonies exposed to high thermal stress. Colonies representative of diverse coral taxa associated with Durusdinium trenchii or Cladocopium spp. exhibited similar nutrient exchange under ambient conditions. In contrast, heat-exposed colonies with D. trenchii experienced less physiological stress than conspecifics with Cladocopium spp. while high carbon assimilation and host transfer was maintained. This discovery is different from the prevailing notion that these mutualisms inevitably suffer trade-offs in physiological performance. These findings emphasize that certain host-symbiont combinations adapted to high temperature equatorial environments; and why their increase in prevalence is likely important to the future productivity and stability of coral reef ecosystems.\n\ncdm_data_type = Other\nVARIABLES:\nlatitude (degrees_north)\nlongitude (degrees_east)\nSite (unitless)\nSpecies (unitless)\nSymbiont (unitless)\nTemperature (degrees Celsius)\nReplicate (unitless)\nFraction (unitless)\nAP13C (percent)\nAP15N (percent)\nFv_Fm (unitless)\nDensities (cells per squared centimeter (cells cm-2))\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_907003_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_907003_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_907003_v1/index.htmlTable https://www.bco-dmo.org/dataset/907003 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_907003_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_907003_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_907003_v1

 
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