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https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_934800_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_934800_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_934800_v1/ public [Three-Prime Tag-Sequencing (3' Tag-Seq) Data for Pisaster ochraceus] - Bioproject accession information on tag-sequence data for Pisaster ochraceus samples collected from Bodega Bay, CA, in July 2019 (Collaborative Proposal: Selection and Genetic Succession in the Intertidal -- Population Genomics of Pisaster ochraceus During a Wasting Disease Outbreak and its Aftermath) Outbreaks of sea star wasting (SSW) have killed millions of sea stars across over 20 taxa in the last decade alone, threatening the health and stability of coastal communities around the world. While the causative agent remains unknown, it has recently been postulated that hypoxia exposure may play a dominant role in the onset of SSW. We leveraged a study that subjected ochre sea stars to organic matter amendment in a controlled laboratory setting to induce hypoxia and used a repeated sampling design to collect non-invasive tissue samples from both healthy and wasting individuals. Following tag-based RNAseq (TagSeq), we analyzed differential gene expression (DGE) patterns among and within these individuals sampled strategically throughout the 15-day experiment. Transcriptional profiles reveal a progressive change in gene expression accompanying the advancement of SSW, reflecting a transition from asymptomatic stars to the onset of characteristic SSW lesions that progressively worsen until, in some cases, the star dies of their symptoms. Included in this dataset is the accession information for 89 individual TagSeq samples across 20 individual Pisaster ochraceus sea stars at multiple time points during the study to make them available for subsequent re-evaluation. The sequence data have been deposited into the NCBI archive under BioProject PRJNA1116313 and will be publicly available on 2025-08-01.\n\ncdm_data_type = Other\nVARIABLES:\nsample_name (unitless)\nbioproject_accession (unitless)\nbioproject_ncbi (unitless)\nassay_type (unitless)\norganism (unitless)\nisolate_id (unitless)\nisolation_source (unitless)\ncollection_date (unitless)\ngeo_loc_name (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\ntissue (unitless)\nbiomaterial_provider (unitless)\ncollected_by (unitless)\nhost_tissue_sampled (unitless)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_934800_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_934800_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_934800_v1/index.htmlTable https://www.bco-dmo.org/dataset/934800 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_934800_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_934800_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_934800_v1

 
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