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https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_928039_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_928039_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_928039_v1/ public [Controlled laboratory study using model organisms Micromonas commoda RCC 299 and Ruegeria pomeroyi DSS-3] - Bacterial transcriptional response to picoeukaryote Micromonas commoda (Effects of Climate Change Variables on Microbial Autotroph-Heterotroph Carbon Flux) Marine biogeochemical cycles are built on interactions between surface ocean microbes, particularly those connecting phytoplankton primary producers to heterotrophic bacteria. However, direct influences of bacteria on phytoplankton physiology are poorly known. In this study, three marine bacteria (Ruegeria pomeroyi DSS-3, Stenotrophomonas sp. SKA14, and Polaribacter dokdonensis MED152) were co-cultured with green alga Micromonas commoda, and the phytoplankter's transcriptome was studied by RNASeq. The presence of each bacterium invoked transcriptomic remodeling by M. commoda after 8 h in co-culture. Some aspects of the algal transcriptomic response were conserved across all three bacteria, while others were restricted to a single bacterium. M. commoda had both rapid and extensive responses to heterotrophic bacteria.\n\ncdm_data_type = Other\nVARIABLES:\nBottle_ID (unitless)\nTreatment (unitless)\nTime_h (hour (h))\nMicromonas_cells_ml (cells per milliliter (cell/ml))\nBacteria_cells_ml (cells per milliliter (cell/ml))\nNH4_uM (micromolar (uM))\nNO3_uM (micromolar (uM))\nPO4_uM (micromolar (uM))\nNCBI_Sample_ID (unitless)\nAccession (unitless)\nBioProject (unitless)\nOrganism (unitless)\nTaxonomy_ID (unitless)\nDescription (unitless)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_928039_v1/index.htmlTable https://www.bco-dmo.org/dataset/928039 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_928039_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_928039_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_928039_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_628993.subset https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_628993 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_628993.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_628993/ public [Incubation in diffuse flow vent fluids - Crab Spa] - Results from shipboard high-pressure incubations of diffuse flow vent fluids collected from the Crab Spa and Alvinella sites at East Pacific Rise during the AT26-10 expedition, Jan. 2014 (Microbial Communities at Deep-Sea Vents project) (An Integrated Study of Energy Metabolism, Carbon Fixation, and Colonization Mechanisms in Chemosynthetic Microbial Communities at Deep-Sea Vents) This dataset includes results from shipboard high-pressure incubations of\ndiffuse flow vent fluids collected from the Crab Spa (9.8398\\u00ba N,\n104.2913\\u00ba W) and Alvinella (9.8398\\u00ba N, 104.2915\\u00ba W) sites at\nEast Pacific Rise during the AT26-10 oceanographic expedition in January 2014.\nReported parameters include dates and time elapsed, flow rate, temperature,\npressure, and pH, and concentrations of NO3, NH4, H2, H2S, CH4.\n \nVent fluids used in shipboard incubations were corrected from diffuse flow\nvent sites at the East Pacific Rise (2503 m): Crab Spa (9.8398\\u00ba N,\n104.2913\\u00ba W) and Alvinella (9.8398\\u00ba N, 104.2915\\u00ba W) (see\ndescription in McNichol et al. [2016]). Fluids were collected using isobaric\ngas-tight samplers [Seewald et al., 2002] prior to their transfer to the\nshipboard continuous culture system [Foustoukos and Perez-Rodriguez, 2015].\nHere, high-pressure incubations (250 bars) were conducted at mesophilic (30\n\\u00baC) and thermophilic (50 \\u00baC) conditions to constrain the function\nand metabolic rates of denitrifying and DNRA microbial communities residing at\nCrab Spa and Alvinella, respectively. To enhance the activity of nitrate-\nrespiring anaerobic bacteria, an NO3- (5 mm) and H2(aq) (1.30 mM)-enriched\nmedium was introduced in the high-pressure incubations under strictly\nanaerobic conditions. Dissolved HCO3- (7.3 mm, 13C labeled) was used as added\ncarbon source. Vent fluids were introduced at a flow rate of 0.042 mL/min,\nwhile growth medium was added at a rate of 0.0042 mL/min. The two sets of\nexperiments were performed for 356 (Crab Spa) and 50 hours (Alvinella). Direct\ncell counts were conducted by staining cells with 0.1% acridine orange and\ncounting them with a fluorescence microscope. 15N/14N isotopic analysis of the\nNO3-, NH4+ and biomass were conducted with a Thermo Scientific Delta VPlus\nmass spectrometer and CE Instruments NA 2500 series elemental analyzer (EA).\n \nReferences:\n \nFoustoukos, D., and I. Perez-Rodriguez (2015), A continuous culture system for\nassessing microbial activities in the piezosphere, Applied and Environmental\nMicrobiology, 81(19), 6850-6856.\n \nMcNichol, J., S. P. Sylva, F. Thomas, C. D. Taylor, S. M. Sievert, and J. S.\nSeewald (2016), Assessing microbial processes in deep-sea hydrothermal systems\nby incubation at in situ temperature and pressure, Deep Sea Research Part I:\nOceanographic Research Papers, 115, 221-232.\n \nSeewald, J. S., K. W. Doherty, T. R. Hammar, and S. P. Liberatore (2002), A\nnew gas-tight isobaric sampler for hydrothermal fluids, Deep-Sea Research,\nPart I: Oceanographic Research Papers, 49(1), 189-196.\n\ncdm_data_type = Other\nVARIABLES:\ndescription (unitless)\n... (15 more variables)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_628993/index.htmlTable https://www.bco-dmo.org/dataset/628993 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_628993.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_628993&showErrors=false&email= BCO-DMO bcodmo_dataset_628993

 
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