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| griddap | Subset | tabledap | Make A Graph | wms | files | Accessible | Title | Summary | FGDC | ISO 19115 | Info | Background Info | RSS | Institution | Dataset ID | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_928039_v1 | https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_928039_v1.graph | https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_928039_v1/ | public | [Controlled laboratory study using model organisms Micromonas commoda RCC 299 and Ruegeria pomeroyi DSS-3] - Bacterial transcriptional response to picoeukaryote Micromonas commoda (Effects of Climate Change Variables on Microbial Autotroph-Heterotroph Carbon Flux) | Marine biogeochemical cycles are built on interactions between surface ocean microbes, particularly those connecting phytoplankton primary producers to heterotrophic bacteria. However, direct influences of bacteria on phytoplankton physiology are poorly known. In this study, three marine bacteria (Ruegeria pomeroyi DSS-3, Stenotrophomonas sp. SKA14, and Polaribacter dokdonensis MED152) were co-cultured with green alga Micromonas commoda, and the phytoplankter's transcriptome was studied by RNASeq. The presence of each bacterium invoked transcriptomic remodeling by M. commoda after 8 h in co-culture. Some aspects of the algal transcriptomic response were conserved across all three bacteria, while others were restricted to a single bacterium. M. commoda had both rapid and extensive responses to heterotrophic bacteria.\n\ncdm_data_type = Other\nVARIABLES:\nBottle_ID (unitless)\nTreatment (unitless)\nTime_h (hour (h))\nMicromonas_cells_ml (cells per milliliter (cell/ml))\nBacteria_cells_ml (cells per milliliter (cell/ml))\nNH4_uM (micromolar (uM))\nNO3_uM (micromolar (uM))\nPO4_uM (micromolar (uM))\nNCBI_Sample_ID (unitless)\nAccession (unitless)\nBioProject (unitless)\nOrganism (unitless)\nTaxonomy_ID (unitless)\nDescription (unitless)\n | https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_928039_v1/index.htmlTable | https://www.bco-dmo.org/dataset/928039
| https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_928039_v1.rss | https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_928039_v1&showErrors=false&email= | BCO-DMO | bcodmo_dataset_928039_v1 | |||||
| https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_628993.subset | https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_628993 | https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_628993.graph | https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_628993/ | public | [Incubation in diffuse flow vent fluids - Crab Spa] - Results from shipboard high-pressure incubations of diffuse flow vent fluids collected from the Crab Spa and Alvinella sites at East Pacific Rise during the AT26-10 expedition, Jan. 2014 (Microbial Communities at Deep-Sea Vents project) (An Integrated Study of Energy Metabolism, Carbon Fixation, and Colonization Mechanisms in Chemosynthetic Microbial Communities at Deep-Sea Vents) | This dataset includes results from shipboard high-pressure incubations of\ndiffuse flow vent fluids collected from the Crab Spa (9.8398\\u00ba N,\n104.2913\\u00ba W) and Alvinella (9.8398\\u00ba N, 104.2915\\u00ba W) sites at\nEast Pacific Rise during the AT26-10 oceanographic expedition in January 2014.\nReported parameters include dates and time elapsed, flow rate, temperature,\npressure, and pH, and concentrations of NO3, NH4, H2, H2S, CH4.\n \nVent fluids used in shipboard incubations were corrected from diffuse flow\nvent sites at the East Pacific Rise (2503 m): Crab Spa (9.8398\\u00ba N,\n104.2913\\u00ba W) and Alvinella (9.8398\\u00ba N, 104.2915\\u00ba W) (see\ndescription in McNichol et al. [2016]). Fluids were collected using isobaric\ngas-tight samplers [Seewald et al., 2002] prior to their transfer to the\nshipboard continuous culture system [Foustoukos and Perez-Rodriguez, 2015].\nHere, high-pressure incubations (250 bars) were conducted at mesophilic (30\n\\u00baC) and thermophilic (50 \\u00baC) conditions to constrain the function\nand metabolic rates of denitrifying and DNRA microbial communities residing at\nCrab Spa and Alvinella, respectively. To enhance the activity of nitrate-\nrespiring anaerobic bacteria, an NO3- (5 mm) and H2(aq) (1.30 mM)-enriched\nmedium was introduced in the high-pressure incubations under strictly\nanaerobic conditions. Dissolved HCO3- (7.3 mm, 13C labeled) was used as added\ncarbon source. Vent fluids were introduced at a flow rate of 0.042 mL/min,\nwhile growth medium was added at a rate of 0.0042 mL/min. The two sets of\nexperiments were performed for 356 (Crab Spa) and 50 hours (Alvinella). Direct\ncell counts were conducted by staining cells with 0.1% acridine orange and\ncounting them with a fluorescence microscope. 15N/14N isotopic analysis of the\nNO3-, NH4+ and biomass were conducted with a Thermo Scientific Delta VPlus\nmass spectrometer and CE Instruments NA 2500 series elemental analyzer (EA).\n \nReferences:\n \nFoustoukos, D., and I. Perez-Rodriguez (2015), A continuous culture system for\nassessing microbial activities in the piezosphere, Applied and Environmental\nMicrobiology, 81(19), 6850-6856.\n \nMcNichol, J., S. P. Sylva, F. Thomas, C. D. Taylor, S. M. Sievert, and J. S.\nSeewald (2016), Assessing microbial processes in deep-sea hydrothermal systems\nby incubation at in situ temperature and pressure, Deep Sea Research Part I:\nOceanographic Research Papers, 115, 221-232.\n \nSeewald, J. S., K. W. Doherty, T. R. Hammar, and S. P. Liberatore (2002), A\nnew gas-tight isobaric sampler for hydrothermal fluids, Deep-Sea Research,\nPart I: Oceanographic Research Papers, 49(1), 189-196.\n\ncdm_data_type = Other\nVARIABLES:\ndescription (unitless)\n... (15 more variables)\n | https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_628993/index.htmlTable | https://www.bco-dmo.org/dataset/628993
| https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_628993.rss | https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_628993&showErrors=false&email= | BCO-DMO | bcodmo_dataset_628993 | ||||
| log in | [nGoM benthic chamber between July 2021 and July 2022] - Benthic chamber geochemical data obtained with an in situ benthic lander from the R/V Savannah at sampling stations across the Louisiana shelf and slope in the Northern Gulf of Mexico during four cruise between July 2021 and July 2022 (Importance of Riverine Discharge on the Benthic Flux of Alkalinity to Continental Margins) | This data set reports benthic flux measurements of dissolved inorganic carbon (DIC), orthophosphate (SPO43-), ammonium (NH4+), total alkalinity (TA), carbon isotopic fractionation of DIC (13-C-DIC), dissolved manganese (Mnd), dissolved calcium (Cad), nitrate (NO3-), and excess bromide (Br-) injected in the chamber as chemical tracer. \n \nIn summer 2021, benthic flux data were obtained from benthic chamber deployments at eleven different stations on the Louisiana Shelf and slope in the Northern Gulf of Mexico during July 15-27, 2021 using two different benthic landers. These stations span two transects, one from the middle of the shelf offshore from Cocodrie, LA to the mouth of the Mississippi River North West Pass, and another from North West Pass of the Mississippi River mouth to the south, across the slope. A single station (St. 15) was also sampled on the eastern side of the second transect in the transition from the shelf break to the mid-slope. All benthic chamber samples were processed within an hour after collection. \n \nIn fall 2021, benthic flux data were obtained from benthic chamber deployments at ten different stations on the Louisiana Shelf and slope in the Northern Gulf of Mexico between October 25 and November 10, 2021 using two different benthic landers. These stations span two transects, one from the middle of the shelf offshore from Cocodrie, LA to the mouth of the Mississippi River South West Pass, and another from South West Pass of the Mississippi River mouth to the south, across the slope. Two stations (St. 15 and St. 12) were also sampled on a second transect from the shelf break to the slope southeast of the Bird's Foot. \n \nIn spring 2022, benthic flux data were obtained from benthic chamber deployments at ten different stations on the Louisiana Shelf and slope in the Northern Gulf of Mexico during April 9-20, 2021 using two different benthic landers. These stations span two transects, one from the middle of the shelf offshore from Cocodrie, LA to the mouth of the Mississippi River South West Pass, and another from South West Pass of the Mississippi River mouth to the south, across the slope. Two stations (St. 15 and St. 12) were also sampled on a second transect from the shelf break to the slope southeast of the Bird's Foot. \n \nIn summer 2022, benthic flux data were obtained from benthic chamber deployments at eleven different stations on the Louisiana Shelf and slope in the Northern Gulf of Mexico in July 9 -23, 2022 using two different benthic landers. These stations span two transects, one from the middle of the shelf offshore from Cocodrie, LA to the mouth of the Mississippi River South West Pass, and another from South West Pass of the Mississippi River mouth to the south, across the slope. Two stations (St. 15 and St. 12) were also sampled on a second transect from the shelf break to the slope southeast of the Bird's Foot. \n \nSamples were immediately filtered through 0.22-micrometer (µm) PSE syringe filters and either analyzed immediately onboard (SPO43-), preserved acidified with hydrochloric acid at 4 degrees Celsius (Cad, Mnd), frozen (NH4+, NO3-, Br-), or preserved at 4 degrees Celsius after addition of HgCl2 (TA) until analysis. In addition, samples for DIC, TA, and carbon isotopic analyses were preserved in glass bottles, whereas other samples were preserved in polypropylene containers. DIC and TA were analyzed by Gran titration (Gran, 1952) using temperature-controlled closed cells (Dickson et al. 2007), orthophosphate and ammonium by spectrophotometry (Murphy and Riley, 1962; Strickland and Parsons, 1972), the carbon isotopic signature by isotope ratio mass spectrometry (Wang et al, 2018), Mnd and Cad by ICP-MS (Magette et al., 2025 In review), and NO3- and Br- by high performance liquid chromatography (HPLC) (Beckler et al., 2014). In summer 2022, benthic chamber and pore water DIC and TA samples were analyzed by cavity ring-down spectrometry (CRDS, Picarro G2131-i) with an automatic CO2 extraction system (Apollo SciTech AS-D1) and open-cell potentiometric titrations, respectively (Ferreira et al., 2025).\n\ncdm_data_type = Other\nVARIABLES:\nDate (unitless)\n... (28 more variables)\n | BCO-DMO | bcodmo_dataset_959033_v1 |