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log in [18S rRNA Gene Amplicons from Swab and Tissue Specimens] - GenBank accessions for 18S rRNA gene amplicons from swab and tissue specimens collected in the Caribbean and Réunion (France) from May 2022 to Dec 2024 (Exploring the role of boundary layer microbial remineralization in flavivirus-host dynamics) Investigating the cause of mass mortality of Diadema antillarum (urn:lsid:marinespecies.org:taxname:124332) identified the etiological agent as the Diadema antillarum Scuticociliatosis Philaster Clade (DaScPc). We sought to investigate the prevalence of this ciliate in sympatric reservoirs in the coral reef environment by targeted PCR using primers designed around this 18S rRNA sequence. We used swab sampling of corals, macroalgae, and other surfaces, as well as urchin tissues, followed by phylogenetic analyses, to identify the presence and diversity of this ciliate. We found that DaScPc could be found primarily in association with the coral Siderastrea siderea, and that changes in its prevalence could be explained by disease state of urchins, macroalgal density, and proximity to disease sites. This dataset contains the GenBank accession, sequencing, and collection metadata for the study.\n\ncdm_data_type = Other\nVARIABLES:\nGenbank_Accession (unitless)\nSequence_ID (unitless)\nSample_Location (unitless)\nSample_Type (unitless)\nSample_Month (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\n BCO-DMO bcodmo_dataset_985574_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_927046_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_927046_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_927046_v1/ public [Dissolved Organic Matter Sulfur and Carbon Analysis] - Dissolved organic matter sulfur and carbon analysis of samples collected between 2010 and 2021 from various locations globally (Resolving sources of marine DOM via novel sulfur isotope analyses) The following dataset of SPE (solid phase extracted) DOM (dissolved organic matter) accompanies Phillips et al. 2022 (doi: 10.1073/pnas.2209152119). Our project sought to address the question of where long-lived organic molecules that are dissolved in the oceans come from, in particular molecules containing sulfur (S). Our approach was to measure the relative abundance of two stable sulfur isotopes (S-32 and S-34) in these molecules, which is technically very difficult due to the presence of million-fold higher sulfate ions in seawater. We developed a new preparatory chemistry to adequately isolate these organic molecules, and a new elemental analyzer/mass spectrometry method to measure their isotope abundances with high precision at trace levels. We conducted these S isotope measurements on 100 samples of dissolved organic matter (DOM) that had been previously collected by our collaborators from around the world (Northeast Pacific oxygen minimum zone, Northeast Pacific Shelf, North Pacific Gyre, San Pedro Basin, Caeté Estuary, South Pacific Gyre, and the North Sea). We also collected 2 dozen new samples from oceanographic stations in the North Pacific Gyre (Hawaii Ocean Timeseries) and North Atlantic Gyre (Bermuda Atlantic Time Series). The dataset includes 1) sample information such as sample ID, sample location, station name, collection depth (ranging from 0 to 4800 meters), latitude and longitude, month and year sampled (ranging from 2010 to 2021); 2) elemental analysis such as sulfur isotope values (δ34S), carbon (C) isotope values (δ13C), and C:S molar ratios; 3) physical parameters from collaborators' CTD analysis, such as oxygen, salinity, fluorescence, and temperature; and 4) chemical data from collaborators such as dissolved nitrate, phosphate, silicate, and calculated DOC (dissolved organic carbon) and DOS (dissolved organic sulfur) concentrations. Our data show that DOM molecules have (34S/32S) isotope ratios that are entirely consistent with being formed from ocean sulfate, and inconsistent with being formed by reactions of hydrogen sulfide in anoxic porewaters. This result negates one of the leading hypotheses for how long-lived DOM forms, i.e. by reactions in anoxic sediments. Instead, this sharpens our focus on understanding how relatively short-lived biomolecules in the surface ocean are transformed into long-lived DOM molecules.\n\ncdm_data_type = Other\nVARIABLES:\nSample_ID (unitless)\nSample_Location (unitless)\nStation (unitless)\n... (23 more variables)\n https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_927046_v1/index.htmlTable https://www.bco-dmo.org/dataset/927046 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_927046_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_927046_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_927046_v1

 
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