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https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_908689_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_908689_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_908689_v1/ public [Gulf of Alaska copepods: annotated transcriptomes] - Annotated de novo transcriptomes generated from six co-occurring species of calanoid copepods from the R/V Tiglax TXF18, TXS19, TXF15, TXF17 in the Gulf of Alaska from 2015-2019 (Collaborative Proposal: Optimizing Recruitment of Neocalanus copepods through Strategic Timing of Reproduction and Growth in the Gulf of Alaska) The dataset includes the annotation files of nine high-quality de novo transcriptomes generated from shotgun assemblies of short-sequence reads. The species are ecologically-important members of sub-arctic North Pacific marine zooplankton communities. The de novo assemblies included one generated several years ago plus eight new ones generated from six co-occurring species of calanoid copepods in the Gulf of Alaska. The transcriptomes include the first published ones for Neocalanus plumchrus, Neocalanus cristatus, Eucalanus bungii and Metridia pacifica and three for Neocalanus flemingeri and two for Calanus marshallae. Total RNA from single individuals was used to construct gene libraries that were sequenced on an Illumina Next-Seq platform. Short-sequence reads were assembled with Trinity software and resulting transcripts were annotated using the SwissProt database with additional functional annotation using gene ontology terms and enzyme function. The annotations files are the first ones published for these species. The integrated dataset can be used for quantitative inter- and intra-species comparisons of gene expression patterns across biological processes using the annotations.\n\nThese data are further described in the following publications: Hartline, et al. (2023) (DOI: 10.1038/s41597-023-02130-1), Roncalli, et al. (2022) (DOI: 10.1111/mec.16354), and Roncalli, et al. (2019) (DOI: 10.1038/s42003-019-0565-5)\n\ncdm_data_type = Other\nVARIABLES:\nseq_id (unitless)\nGenbank_accession (unitless)\nSpecies (unitless)\nStation (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\nCollection_date (unitless)\nDepth_range (meters (m))\nLife_stage (unitless)\nSex (unitless)\nEntry (unitless)\n... (20 more variables)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_908689_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_908689_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_908689_v1/index.htmlTable https://www.bco-dmo.org/dataset/908689 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_908689_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_908689_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_908689_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_914459_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_914459_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_914459_v1/ public [Neocalanus flemingeri incubation experiment:  gene expression] - Gene expression profiles for Neocalanus flemingeri pre adults (CV) exposed to four different experimental food conditions collected from the M/V Dora in the Gulf of Alaska at station GAK1 from April 2019 (Collaborative Research: Molecular profiling of the ecophysiology of dormancy induction in calanid copepods of the Northern Gulf of Alaska LTER site) This experimental dataset includes relative expression of individual Neocalanus flemingeri stage CV individuals incubated for different lengths of time and four different food treatments. The experimental protocol and results are described in detail in Roncalli et al., 2023. Briefly, field-collected N. flemingeri were allowed to molt into stage CV and then sorted into four different treatments: no food, low carbon, high carbon and high carbon with diatoms. After a one-week incubation, individuals from all four treatments were processed individually for RNA-Seq. In addition, following two and three-week incubations, copepods from the three fed treatments were processed individually for RNA-Seq. Short-sequence reads were mapped against a reference transcriptome and normalized gene expression was computed for each transcript. The dataset includes log-transformed relative gene expression in reads per kilobase per million reads (RPKM) (log2[RPKM+1]). The dataset also includes a list of differentially expressed genes and a look-up table that cross-references the hierarchical identifications of transcripts generated by the Trinity assembly software and the corresponding National Center for Biotechnology Information (NCBI) accession number. \n\nThese data are further described in the following publications: Roncalli, et al. (2023) (DOI: 10.1093/plankt/fbad045) and Roncalli, et al. (2019) (DOI: 10.1038/s42003-019-0565-5)\n\ncdm_data_type = Other\nVARIABLES:\nseq_id (unitless)\nGenbank_accession (unitless)\nOrganism_Name (unitless)\nStation (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\nCollection_date (unitless)\nYear (unitless)\nMonth (unitless)\nDay (unitless)\nDepth_range (meters (m))\n... (33 more variables)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_914459_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_914459_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_914459_v1/index.htmlTable https://www.bco-dmo.org/dataset/914459 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_914459_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_914459_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_914459_v1

 
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