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https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_954439_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_954439_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_954439_v1/ public [Caribbean sponges - nanoSIMS] - NanoSIMS data from sponges collected in Summerland Key in Florida between July 27 - August 19, 2021 (Collaborative Research: Investigations into microbially mediated ecological diversification in sponges) Sponges represent one of the oldest extant animal phyla, and their associations with microbial symbionts have likely played a critical role in their success on oligotrophic coral reefs. For example, variation in the abundance, diversity, and community composition of microbiomes across host species may drive niche partitioning due to differential assimilation and recycling of nutrients across sponge species. With Nanoscale secondary ion mass spectrometry (NanoSIMS), we evaluated the respective roles of host and microbial symbiont cells in the uptake and recycling of three resource pools (dissolved organic matter: DOM, particulate organic matter: POM, and inorganic nutrients: NaHCO3 and NaNO3) over 72 hr. NanoSIMS analysis revealed enrichment of individual host and symbiont cells over time and highlighted the differing roles of host and symbiont cells in the uptake and recycling of diverse sources of carbon and nitrogen within two of these species. For instance, dissolved organic matter uptake was mediated by microbial symbionts in one species and by host cells in another species. Together , these results demonstrate that associations with microbial symbionts have facilitated niche partitioning by allowing host sponges to acquire and recycle diverse nutrient pools via unique mechanisms. Field and lab work for this research was carried out in the Florida Keys during June/August of 2021.\n\ncdm_data_type = Other\nVARIABLES:\nRoi_name (unitless)\nRoi_group (unitless)\narea (pixels^2)\nmean13C (ratio (x10000))\nstddev13C (ratio (x10000))\nR_13C12C_12C2 (ratio)\ncorr_R_13C_13C (ratio)\nd_13C_12C (delta 13C in permille)\nspecies (unitless)\nCollection_date (unitless)\ntime_sample (Time, unitless)\nSEM_region (unitless)\nmean15N (ratio (x10000))\n... (7 more variables)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_954439_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_954439_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_954439_v1/index.htmlTable https://osprey.bco-dmo.org/dataset/954439 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_954439_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_954439_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_954439_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_954470_v1 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_954470_v1.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_954470_v1/ public [Carribean sponges - bulk isotopes] - Bulk isotope data from sponges collected in Summerland Key in Florida between July 27 - August 19, 2021 (Collaborative Research: Investigations into microbially mediated ecological diversification in sponges) Sponges represent one of the oldest extant animal phyla, and their associations with microbial symbionts have likely played a critical role in their success on oligotrophic coral reefs. For example, variation in the abundance, diversity, and community composition of microbiomes across host species may drive niche partitioning due to differential assimilation and recycling of nutrients across sponge species. Stable isotope analysis of bulk sponge tissue allowed us to test for evidence of partitioning of three major resource pools (dissolved organic matter: DOM, particulate organic matter: POM, and inorganic nutrients: NaHCO3 and NaNO3) among four emergent Caribbean sponge species. Results from bulk (holobiont tissue including both sponge and microbial cells) stable isotope analysis show niche partitioning, with strong interspecific variation in the relative exploitation of each resource pool. These results demonstrate that associations with microbial symbionts have facilitated niche partitioning by allowing host sponges to acquire and recycle diverse nutrient pools via unique mechanisms.\n\ncdm_data_type = Other\nVARIABLES:\nExperiment (unitless)\ntime_sample (Time, unitless)\nreplicate (unitless)\nspecies_abbreviation (unitless)\nSpecies_name (unitless)\nCollection_date (unitless)\nlatitude (degrees_north)\nlongitude (degrees_east)\nLinear_corr_d15N (permille (‰))\nwt_perc_N (percentag (%))\nAtm_perc_15N (percentag (%))\nLinear_corr_d13C (permille (‰))\nwt_perc_C (percentag (%))\nAtm_perc_13C (percentag (%))\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_954470_v1_fgdc.xml https://erddap.bco-dmo.org/erddap/metadata/iso19115/xml/bcodmo_dataset_954470_v1_iso19115.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_954470_v1/index.htmlTable https://osprey.bco-dmo.org/dataset/954470 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_954470_v1.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_954470_v1&showErrors=false&email= BCO-DMO bcodmo_dataset_954470_v1
log in [Chemotaxis of V. alginolyticus towards Synechococcus Cells] -  (Collaborative Research: Viral induced chemotaxis mediating cross-trophic microbial interactions and carbon flux) This data set summarises the chemotactic response of model heterotrophic bacteria, Vibrio alginolyticus towards phage-infected cyanobacteria Synechococcus cells/exudates respectively. Phage treatments are fully described in: https://doi.org/10.1038/s43705-022-00169-6.\n\nSix microfluidic experiments were conducted each on different days: three with phage infection and three control, uninfected experiments. Both uninfected and phage-infected experiments were performed identically, with the substitution of phage addition for an equivalent volume of SN media in the uninfected experiments. All infection experiments were performed within a week using the same phage stock, with Synechococcus WH8102 as the host. Non-motile host cells were loaded into a microfluidic chamber with the model heterotrophic bacteria V. alginolyticus. \n\nAnalysis of the cell spatial distribution over time revealed a strong sustained accumulation of chemotactic bacteria towards phage-infected cyanobacteria, and no measurable accumulation in the control condition. This provides the first direct experimental evidence of chemotaxis of heterotrophic bacteria towards cyanobacteria.\n\ncdm_data_type = Other\nVARIABLES:\ntime_sample (Time, minutes)\nINF1_4HPI (unitless)\nINF2_4HPI (unitless)\nINF3_4HPI (unitless)\nINF_4HPI_AVG (unitless)\nINF_4HPI_SEM (unitless)\nINF1_8HPI (unitless)\nINF2_8HPI (unitless)\nINF3_8HPI (unitless)\nINF_8HPI_AVG (unitless)\nINF_8HPI_SEM (unitless)\nUI1_4HPI (unitless)\nUI2_4HPI (unitless)\n... (8 more variables)\n BCO-DMO bcodmo_dataset_913619_v1
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_3300 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_3300.graph https://erddap.bco-dmo.org/erddap/files/bcodmo_dataset_3300/ public [larval krill pigments] - Larval krill studies - fluorescence and clearance from ARSV Laurence M. Gould LMG0106, LMG0205 in the Southern Ocean from 2001-2002 (SOGLOBEC project) (U.S. GLOBEC Southern Ocean) The goal of the larval krill studies was to investigate the physiology and ecology of krill larvae associated with the pack ice and the microbial community on which they feed.\\r\\n\\r\\nDuring LMG0106 we occupied two 4-5 day ice stations (Robert and Billy) and sampled several other ice floes opportunistically. We conducted 10 instantaneous growth rate experiments, and 4 whole body clearance time experiments to determine gut passage time (decline in pigment content over time). We also sampled larvae at two additional sites for initial body pigment content (whole body fluorescence), and at 4 sites for condition factor. The under-ice algal community was sampled at one site. Length and stage frequency determinations were also determined.\\r\\n\\r\\nWe occupied three time-series stations of approximately 1 week each, and in addition opportunistically sampled at times when other activities had priority. Our primary goal during the cruise was to occupy three ice camps or process stations with the intent of thoroughly studying the under-ice environment by SCUBA in conjunction with other projects working topside. (from cruise report LMG0205)\n\ncdm_data_type = Other\nVARIABLES:\ncruiseid (text)\nyear (unitless)\nsample_id (alpha-numeric)\nlatitude (degrees_north)\nlongitude (degrees_east)\nday_local\nmonth_local\ntime_local\nyrday_local\ntime_sample (minutes)\npigment_content (micrograms total chl/grams wet weight)\nstage_id\nwet_weight (mg)\n https://erddap.bco-dmo.org/erddap/metadata/fgdc/xml/bcodmo_dataset_3300_fgdc.xml https://erddap.bco-dmo.org/erddap/info/bcodmo_dataset_3300/index.htmlTable https://www.bco-dmo.org/dataset/3300 (external link) https://erddap.bco-dmo.org/erddap/rss/bcodmo_dataset_3300.rss https://erddap.bco-dmo.org/erddap/subscriptions/add.html?datasetID=bcodmo_dataset_3300&showErrors=false&email= BCO-DMO bcodmo_dataset_3300

 
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