BCO-DMO ERDDAP
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Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Full details of the experimental design are provided in Hawkins et al., 2016
attribute NC_GLOBAL awards_0_award_nid String 628966
attribute NC_GLOBAL awards_0_award_number String EF-1316055
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1316055 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Emerging Frontiers Division
attribute NC_GLOBAL awards_0_funding_acronym String NSF EF
attribute NC_GLOBAL awards_0_funding_source_nid String 392
attribute NC_GLOBAL awards_0_program_manager String Irwin Forseth
attribute NC_GLOBAL awards_0_program_manager_nid String 520504
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Sea Anemone respiratory capacity \n   with two different symbionts  \n   in an experimental setting \n   CS = Citrate Synthase \n    M. Warner, PI \n   Version 14 June 16
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3  19 Dec 2019
attribute NC_GLOBAL date_created String 2016-06-17T15:05:21Z
attribute NC_GLOBAL date_modified String 2019-07-02T15:06:36Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.649728.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/649728 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_dataset_instrument_description String [EVOS system, ThermoFisher (Life Technologies), Waltham, MA, USA; excitation: 628 ± 20 nm, emission: 692 ± 20 nm].\nField of view was determined using the EVOS operating software (4× objective), and cells were counted using the “Analyze Particles” tool in ImageJ (NIH, Bethesda, MD, USA).
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 652568
attribute NC_GLOBAL instruments_0_description String Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB06/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Microscope-Fluorescence
attribute NC_GLOBAL instruments_0_instrument_nid String 695
attribute NC_GLOBAL instruments_0_supplied_name String Fluorescence Microscope
attribute NC_GLOBAL instruments_1_dataset_instrument_description String an oxygen sensitive optode (Fibox 4, PreSens Gmbh, Regensburg, Germany).
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 652570
attribute NC_GLOBAL instruments_1_description String An optode or optrode is an optical sensor device that optically measures a specific substance usually with the aid of a chemical transducer.
attribute NC_GLOBAL instruments_1_instrument_name String Optode
attribute NC_GLOBAL instruments_1_instrument_nid String 727
attribute NC_GLOBAL instruments_1_supplied_name String oxygen sensitive optode
attribute NC_GLOBAL instruments_2_acronym String Thermal Cycler
attribute NC_GLOBAL instruments_2_dataset_instrument_description String AB-7500 real-time QPCR system [ThermoFisher (Applied Biosystems), Waltham, MA, USA], with the following cycling conditions: 94◦ C for 10 min, followed by 40 cycles of 94◦C for 15 s, 60◦C for 1 min, and 72◦C for 15 s.
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 652569
attribute NC_GLOBAL instruments_2_description String General term for a laboratory apparatus commonly used for performing polymerase chain reaction (PCR). The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps.\n\n(adapted from http://serc.carleton.edu/microbelife/research_methods/genomics/pcr.html)
attribute NC_GLOBAL instruments_2_instrument_name String PCR Thermal Cycler
attribute NC_GLOBAL instruments_2_instrument_nid String 471582
attribute NC_GLOBAL instruments_2_supplied_name String ThermoFisher QPCR system
attribute NC_GLOBAL keywords String act, anemone, anemone_resp_rate, bco, bco-dmo, biological, biom, biomass, chemical, data, dataset, density, dmo, erddap, fraction, host, host_CS_act, management, oceanography, office, output, photo, photo_output_symb, preliminary, quotient, rate, resp, resp_biom_quotient, sample, symb, symbio, symbio_biomass_fraction, symbio_density, symbio_resp_fraction, symbiont, symbiont_CS_act, symbiont_id
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/649728/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/649728 (external link)
attribute NC_GLOBAL param_mapping String {'649728': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/649728/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Delaware
attribute NC_GLOBAL people_0_person_name String Mark E. Warner
attribute NC_GLOBAL people_0_person_nid String 51387
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_1_person_name String Ms Dicky Allison
attribute NC_GLOBAL people_1_person_nid String 50382
attribute NC_GLOBAL people_1_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL project String AnemoneOA
attribute NC_GLOBAL projects_0_acronym String AnemoneOA
attribute NC_GLOBAL projects_0_description String The projected rise in carbon dioxide (CO2) in the atmosphere is considered a primary threat to marine systems throughout the world due to both ocean acidification and rising ocean temperatures. Coral reefs are very sensitive to these projected changes in the earth's climate, with continued losses in growth as well as disruption (also known as bleaching) in the symbiotic relationship between the algae (Symbiodinium) living within a diversity of host animals, including stony corals, soft corals and sea anemones. While much information has been gleaned as to how acidification may affect stony corals, considerably less is known about the interactive effects of acidification and temperature to other symbiotic anthozoans.\nTo this end, this proposal will investigate the long-term impacts of elevated CO2 and temperature on the model sea anemone, Aiptasia pallida, while harboring four different genotypes of Symbiodinium. The primary goals of this project are (1) to determine the sensitivity and capacity for acclimation in molecular and physiological processes while exposed to elevated CO2 and temperature, and (2) to assess the degree to which acclimated adult animals may confer (or transfer) an imprinted physiological characteristic to the next generation of asexual offspring. A series of long-term experiments will be conducted with each animal/algal combination (holobiont) in order to collect initial (3 month) stress markers and genomic data and then follow animal response and asexual reproduction through several generations for one year. The possibility for enhanced resilience or acclimation will be measured by tracking the recovery of each holobiont, followed by repeated exposure to elevated temperature while held in high CO2. This project will tease apart fine scale mechanisms of stress, acclimation, or amelioration that may vary as a function of algal genotype and host animal response, and the degree to which environmental imprinting may pre-acclimate propagules. Project results will provide information regarding how future acidification and warming will affect cnidarian-algal symbioses, and the fundamental profile of their flexibility in stress response processes across organismal, metabolic, genomic and epigenetic scales.
attribute NC_GLOBAL projects_0_end_date String 2016-06
attribute NC_GLOBAL projects_0_geolocation String Lewes, Delaware
attribute NC_GLOBAL projects_0_name String Ocean Acidification: Understanding the Impact of CO2 and Temperature on the Physiological, Genetic, and Epigenetic Response of a Model Sea Anemone System with Different Symbionts
attribute NC_GLOBAL projects_0_project_nid String 628967
attribute NC_GLOBAL projects_0_start_date String 2013-07
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Symbiont removed and anemone reinfected with one of two strains of Symbiodinium from (AnemoneOA project)
attribute NC_GLOBAL title String [Host-symbiont reinfections] - Symbiont removed and anemone reinfected with one of two strains of Symbiodinium from (AnemoneOA project) (Ocean Acidification: Understanding the Impact of CO2 and Temperature on the Physiological, Genetic, and Epigenetic Response of a Model Sea Anemone System with Different Symbionts)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable sample String
attribute sample bcodmo_name String sample
attribute sample description String Sample ID number
attribute sample long_name String Sample
attribute sample nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute sample units String unitless
variable symbiont_id String
attribute symbiont_id bcodmo_name String taxon
attribute symbiont_id description String Symbiont identity; i.e. Homologous ITS2-type A4 Symbiodinium or Symbiodinium minutum ITS2-type B1
attribute symbiont_id long_name String Symbiont Id
attribute symbiont_id units String text
variable anemone_resp_rate double
attribute anemone_resp_rate _FillValue double NaN
attribute anemone_resp_rate actual_range double 0.813157803, 2.214224466
attribute anemone_resp_rate bcodmo_name String O2 consumption
attribute anemone_resp_rate description String Host protein-normalised holobiont respiration rate
attribute anemone_resp_rate long_name String Anemone Resp Rate
attribute anemone_resp_rate units String micromoles of oxygen per hour per milligram protein
variable host_CS_act double
attribute host_CS_act _FillValue double NaN
attribute host_CS_act actual_range double 0.06816797, 0.185088974
attribute host_CS_act bcodmo_name String metabolic_enzyme
attribute host_CS_act description String Host (anemone) citrate synthase specific activity
attribute host_CS_act long_name String Host CS Act
attribute host_CS_act units String Units per milligram protein
variable symbiont_CS_act double
attribute symbiont_CS_act _FillValue double NaN
attribute symbiont_CS_act actual_range double 0.009343539, 0.13640119
attribute symbiont_CS_act bcodmo_name String metabolic_enzyme
attribute symbiont_CS_act description String Symbiont (Symbiodinium) citrate synthase specific activity
attribute symbiont_CS_act long_name String Symbiont CS Act
attribute symbiont_CS_act units String Units per milligram protein
variable photo_output_symb double
attribute photo_output_symb _FillValue double NaN
attribute photo_output_symb actual_range double 0.184067192, 1.337695877
attribute photo_output_symb bcodmo_name String photosynthesis
attribute photo_output_symb description String Net photosynthesis per symbiont cell
attribute photo_output_symb long_name String Photo Output Symb
attribute photo_output_symb units String picomoles oxygen per hour per cell
variable symbio_density double
attribute symbio_density _FillValue double NaN
attribute symbio_density actual_range double 1098543.993, 8812379.853
attribute symbio_density bcodmo_name String abundance
attribute symbio_density description String Host protein-normalised symbiont density
attribute symbio_density long_name String Symbio Density
attribute symbio_density nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/B070/ (external link)
attribute symbio_density units String million cells per mg protein
variable symbio_biomass_fraction float
attribute symbio_biomass_fraction _FillValue float NaN
attribute symbio_biomass_fraction actual_range float 0.0183, 0.1581
attribute symbio_biomass_fraction bcodmo_name String unknown
attribute symbio_biomass_fraction colorBarMaximum double 1.0
attribute symbio_biomass_fraction colorBarMinimum double 0.0
attribute symbio_biomass_fraction description String Symbiont biomass fraction (calculated as total symbiont protein [mg]/total host+symbiont protein [mg])
attribute symbio_biomass_fraction long_name String Symbio Biomass Fraction
attribute symbio_biomass_fraction units String milligrams per milligrams(dimensionless)
variable symbio_resp_fraction float
attribute symbio_resp_fraction _FillValue float NaN
attribute symbio_resp_fraction actual_range float 0.0041, 0.1433
attribute symbio_resp_fraction bcodmo_name String unknown
attribute symbio_resp_fraction colorBarMaximum double 1.0
attribute symbio_resp_fraction colorBarMinimum double 0.0
attribute symbio_resp_fraction description String Symbiont respiration fraction (calculated as total symbiont citrate synthase activity [U]/total host+symbiont citrate synthase activity [U])
attribute symbio_resp_fraction long_name String Symbio Resp Fraction
attribute symbio_resp_fraction units String dimensionless
variable resp_biom_quotient double
attribute resp_biom_quotient _FillValue double NaN
attribute resp_biom_quotient actual_range double 0.072127984, 1.036789977
attribute resp_biom_quotient bcodmo_name String unknown
attribute resp_biom_quotient description String Symbiont respiration fraction/Symbiont biomass fraction
attribute resp_biom_quotient long_name String Resp Biom Quotient
attribute resp_biom_quotient units String dimensionless

 
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