BCO-DMO ERDDAP
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Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute NC_GLOBAL acquisition_description String Surface sediment was retrieved using a modified Van Veen grab. Duplicate cores\nwere taken from each grab sample using sterile, cut-off 5 mL syringes and\nimmediately placed on dry ice prior to storage at \\u201380 degrees celsius.\nBottom water nutrient samples were collected in triplicate using a hand-held\nNiskin bottle, immediately filtered (0.2 um pore size), and frozen on dry ice\nprior to storage at \\u201320 degrees celsius. Nutrient (NH4+, NO2-, and NO3-)\nconcentrations were measured using a QuikChem 8000 Flow Injection Analyzer\n(Lachat Instruments).\\u00a0\n \nSediment samples for potential nitrification rate measurements were collected\nin triplicate into the barrels of cut-off 60 mL syringes, which were sealed\nwith parafilm and transported to the laboratory on ice. Potential rates were\nmeasured using amended sediment slurries. Slurries included 5 g of sediment\n(top 1 cm) homogenized in 100 mL of filtered bottom water augmented with NH4+\nand phosphate to final additional concentrations of 500 and 100 uM,\nrespectively. Amended slurries were shaken (200 rpm) in the dark for 24 hours\nat room temperature (about 22 degrees celsius). Aliquots for the determination\nof NO3- plus NO2- (NOX) were collected at evenly spaced intervals through the\nincubation period and stored at \\u201320 degrees celsius. Prior to analysis,\naliquots were thawed and passed through Whatman No. 42 filter paper, and the\nfiltrate was analyzed for the accumulation of NOx over time, using a SmartChem\n200 Discrete Analyzer (Unity Scientific). Rates were determined by linear\nregression of NOx concentrations over time.\n \nDNA was extracted from approximately 0.5 g of surface sediments by extruding\nand cutting the top 0.5 cm from frozen cores with a sterile scalpel and\nimmediately proceeding with the FastDNA SPIN Kit for Soil (MP Biomedicals),\nincluding a FastPrep bead beating step of 30 s at speed 5.5. AOA and AOB amoA\ngenes were quantified using gene-specific SYBR qPCR assays on a StepOnePlus\nReal-Time PCR System (Life Technologies). AOA amoA reactions contained iTaq\nSYBR Green Supermix with ROX (Bio-Rad Laboratories), 0.4 uM primers Arch-amoAF\n/Arch-amoAR (Francis et al., 2005) and 1 uL template DNA. AOA qPCR program\ndetails were identical to previously published protocols (Mosier and Francis,\n2008) but with a 10 s detection step at 78.5 degrees celsius. AOB amoA qPCR\nreactions used primers amoA1F/amoA2R (Rotthauwe et al., 1997), and were set up\nfollowing Mosier and Francis (2008) but with a 10 s detection step at 83\ndegress celsius. Each plate included a standard curve (5 to 10^6\ncopies/reaction) made by serial dilution of linearized plasmids extracted from\npreviously sequenced clones, and negative controls that substituted sterile\nwater for DNA. The diversity of ammonia oxidizing communities was determined\nby cloning and sequencing of PCR-amplified amoA genes using primers Arch-amoAF\n/Arch-amoAR (Francis et al., 2005) and amoA1F*/amoA2R (Rotthauwe et al., 1997;\nStephen et al., 1999) for AOA and AOB, respectively. Reaction conditions and\nPCR programs followed previously published protocols (Mosier and Francis,\n2008). Triplicate reactions were qualitatively checked by gel electrophoresis,\npooled, and purified using the MinElute PCR Purification Kit or MinElute Gel\nExtraction Kit (Qiagen), following the manufacturer\\u2019s instructions.\nPurified products were cloned using the pGEM-T Vector System II (Promega), and\nsequenced by Elim Biopharmaceuticals on a 3730xl capillary sequencer (Life\nTechnologies). Sequences were imported into Geneious (version 6.1.6 created by\nBiomatters, available from\n[http://www.geneious.com](\\\\\"http://www.geneious.com\\\\\")) and manually cleaned\nprior to operational taxonomic unit (OTU) grouping (greater than or equal to\n95% sequence similarity) using mothur (Schloss et al., 2009). Rarefaction\ncurves and diversity/richness estimators (Chao1 and Shannon indices) were\ncalculated using mothur. OTUs were aligned with reference sequences using the\nMUSCLE alignment package within Geneious, using a gap open score of \\u2013750.\nAlignments were manually checked and used to build neighbor-joining bootstrap\ntrees (Jukes-Cantor distance model, 1000 neighbor joining bootstrap\nreplicates) within Geneious. The amoA sequences generated in this study have\nbeen deposited into GenBank with accession numbers KM000240 to KM000508 (AOB)\nand KM000509 to KM000784 (AOA).\n \nTwo-tailed Spearman rank correlation coefficients (\\u03c1) were calculated\nusing R (R Core Team, 2014) to determine correlations between variables, using\nthe suggested critical value of 0.786 for 5% significance with a sample size\nof 7 (Zar, 1972). Principal component and non-metric multidimensional scaling\nanalyses were performed using the vegan package in R (Oksanen, 2013).\nEnvironmental variables were z-transformed to standardize across different\nscales and units by subtracting the population mean from each measurement and\ndividing by the standard deviation. OTU count data were Hellinger-transformed\nto standardize to relative abundances (Legendre and Legendre, 2012). Other\nthan unweighted UniFrac distances, which were calculated using the online\nUniFrac portal (Lozupone et al., 2006), distance/dissimilarity indices were\ncalculated using the vegan package in R. All principle component analyses are\npresented using scaling 1; therefore, the distance between sites on the biplot\nrepresents their Euclidean distance, and the right-angle projection of a site\nonto a descriptor vector shows the approximate position of that site on the\nvector (Legendre and Legendre, 2012).\\u00a0
attribute NC_GLOBAL awards_0_award_nid String 546277
attribute NC_GLOBAL awards_0_award_number String OCE-0847266
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=0847266 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Delta Nitrification \n  Christopher A. Francis, PI \n  Version 17 August 2016
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3  19 Dec 2019
attribute NC_GLOBAL date_created String 2016-08-18T19:59:18Z
attribute NC_GLOBAL date_modified String 2019-05-22T14:36:45Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.654371.1
attribute NC_GLOBAL Easternmost_Easting double -121.597033
attribute NC_GLOBAL geospatial_lat_max double 38.167117
attribute NC_GLOBAL geospatial_lat_min double 38.017617
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -121.597033
attribute NC_GLOBAL geospatial_lon_min double -121.850917
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/654371 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Niskin bottle
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Hand-held Niskin bottle
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 654377
attribute NC_GLOBAL instruments_0_description String A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends.  The bottles can be attached individually on a hydrowire or deployed in 12, 24 or 36 bottle Rosette systems mounted on a frame and combined with a CTD.  Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Niskin bottle
attribute NC_GLOBAL instruments_0_instrument_nid String 413
attribute NC_GLOBAL instruments_0_supplied_name String Niskin bottle
attribute NC_GLOBAL instruments_1_acronym String FIA
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Concentrations measured via QuikChem 8000 Flow Injection Analyzer
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 654378
attribute NC_GLOBAL instruments_1_description String An instrument that performs flow injection analysis. Flow injection analysis (FIA) is an approach to chemical analysis that is accomplished by injecting a plug of sample into a flowing carrier stream. FIA is an automated method in which a sample is injected into a continuous flow of a carrier solution that mixes with other continuously flowing solutions before reaching a detector. Precision is dramatically increased when FIA is used instead of manual injections and as a result very specific FIA systems have been developed for a wide array of analytical techniques.
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB36/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Flow Injection Analyzer
attribute NC_GLOBAL instruments_1_instrument_nid String 657
attribute NC_GLOBAL instruments_1_supplied_name String QuikChem 8000
attribute NC_GLOBAL instruments_2_acronym String Thermal Cycler
attribute NC_GLOBAL instruments_2_dataset_instrument_description String Genes quantified using gene-specific SYBR qPCR assays
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 654380
attribute NC_GLOBAL instruments_2_description String General term for a laboratory apparatus commonly used for performing polymerase chain reaction (PCR). The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps.\n\n(adapted from http://serc.carleton.edu/microbelife/research_methods/genomics/pcr.html)
attribute NC_GLOBAL instruments_2_instrument_name String PCR Thermal Cycler
attribute NC_GLOBAL instruments_2_instrument_nid String 471582
attribute NC_GLOBAL instruments_2_supplied_name String StepOnePlus Real-Time PCR System
attribute NC_GLOBAL instruments_3_acronym String Discrete Analyzer
attribute NC_GLOBAL instruments_3_dataset_instrument_description String Filtrate analyzed from the accumulation of NOx over time using this discrete analyzer.
attribute NC_GLOBAL instruments_3_dataset_instrument_nid String 654379
attribute NC_GLOBAL instruments_3_description String Discrete analyzers utilize discrete reaction wells to mix and develop the colorimetric reaction, allowing for a wide variety of assays to be performed from one sample. These instruments are ideal for drinking water, wastewater, soil testing, environmental and university or research applications where multiple assays and high throughput are required.
attribute NC_GLOBAL instruments_3_instrument_name String Discrete Analyzer
attribute NC_GLOBAL instruments_3_instrument_nid String 654337
attribute NC_GLOBAL instruments_3_supplied_name String SmartChem 200 Discrete Analyzer
attribute NC_GLOBAL keywords String ammonia, ammonium, amo, aoa, AOA_amoA, aoato, aob, AOB_amoA, bco, bco-dmo, biological, chemical, chemistry, concentration, data, dataset, date, dmo, earth, Earth Science > Oceans > Ocean Chemistry > Ammonia, Earth Science > Oceans > Ocean Chemistry > Nitrate, erddap, latitude, log, log_AOAtoAOB, longitude, management, mole, mole_concentration_of_ammonium_in_sea_water, mole_concentration_of_nitrate_in_sea_water, mole_concentration_of_nitrite_in_sea_water, n02, nh4, nitrate, nitrification, nitrite, NO2, no3, ocean, oceanography, oceans, office, preliminary, science, sea, seawater, station, time, water
attribute NC_GLOBAL keywords_vocabulary String GCMD Science Keywords
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/654371/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/654371 (external link)
attribute NC_GLOBAL Northernmost_Northing double 38.167117
attribute NC_GLOBAL param_mapping String {'654371': {'lat': 'master - latitude', 'lon': 'master - longitude'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/654371/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Stanford University
attribute NC_GLOBAL people_0_person_name String Dr Christopher Francis
attribute NC_GLOBAL people_0_person_nid String 51553
attribute NC_GLOBAL people_0_role String Lead Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_1_person_name String Hannah Ake
attribute NC_GLOBAL people_1_person_nid String 650173
attribute NC_GLOBAL people_1_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL project String N-Cycling Microbial Communities
attribute NC_GLOBAL projects_0_acronym String N-Cycling Microbial Communities
attribute NC_GLOBAL projects_0_description String Description from the NSF award abstract:\nThis award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).\nAlthough nitrogen (N) acts as a limiting nutrient in many marine ecosystems, from estuaries to the open ocean, N in excess can be extremely detrimental. Eutrophication is of particular concern in estuaries, with over half of the estuaries in the United States experiencing its effects. Harmful levels of N in estuaries can be diminished through tightly coupled processes in the microbial nitrogen cycle, including nitrification (chemoautotrophic oxidation of ammonia to nitrite and nitrate) and denitrification (the dissimilatory reduction of nitrate to N2 gas). In fact, coupled nitrification-denitrification can remove up to 50% of external dissolved inorganic nitrogen inputs to estuaries, thereby reducing the risk of eutrophication. Despite the biogeochemical importance of both nitrification and denitrification in estuarine systems, surprisingly little is known regarding the underlying microbial communities responsible for these processes, or how they are influenced by key physical/chemical factors.\nThe investigators will work in San Francisco Bay - the largest estuary on the west coast of the United States - using molecular, biogeochemical and cultivation approaches to explore how the distribution, diversity, abundance, and activities of key N-cycling communities are influenced by environmental gradients over temporal and spatial scales. Denitrifying communities will be studied using functional genes (nirK and nirS) encoding the key denitrification enzyme nitrite reductase, while genes encoding ammonia monooxygenase subunit A (amoA) will be used to study both ammonia-oxidizing bacteria (AOB) and the recently-discovered ammonia-oxidizing archaea (AOA)- members of one of the most ubiquitous and abundant prokaryotic groups on the planet, the mesophilic Crenarchaeota. Analyzing sediments from sites spanning a range of physical and chemical conditions in the Bay, seasonally over the course of several years, will represent an unprecedented opportunity to examine spatial, physical/chemical, and temporal effects on both denitrifier and ammonia-oxidizer communities in this large, urban estuary. Concurrently, an intensive cultivation effort will also be undertaken, in order to compile a novel culture collection of estuarine denitrifiers and ammonia-oxidizers, for which virtually nothing is currently known. Taken together, these complimentary approaches will help reveal how complex physical/chemical gradients influence the diversity and functioning of key estuarine N-cycling communities over time and space.
attribute NC_GLOBAL projects_0_end_date String 2015-05
attribute NC_GLOBAL projects_0_geolocation String San Francisco Bay
attribute NC_GLOBAL projects_0_name String Spatial and Temporal Dynamics of Nitrogen-Cycling Microbial Communities Across Physicochemical Gradients in the San Francisco Bay Estuary
attribute NC_GLOBAL projects_0_project_nid String 546278
attribute NC_GLOBAL projects_0_start_date String 2009-06
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 38.017617
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Potential nitrification rates and ammonia oxidizer gene abundances collected on R/V Endeavor (SQO-Delta) in the San Francisco Bay Delta during September and October 2007
attribute NC_GLOBAL title String [Delta Nitrification Study - Potential Nitrification Rates] - Potential nitrification rates and ammonia oxidizer gene abundances collected on R/V Endeavor (SQO-Delta) in the San Francisco Bay Delta during September and October 2007 (Spatial and Temporal Dynamics of Nitrogen-Cycling Microbial Communities Across Physicochemical Gradients in the San Francisco Bay Estuary)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -121.850917
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable date String
attribute date bcodmo_name String date
attribute date description String date when sample was taken; mm/dd/yyyy
attribute date long_name String Date
attribute date nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/ (external link)
attribute date source_name String date
attribute date time_precision String 1970-01-01
attribute date units String unitless
variable station String
attribute station bcodmo_name String station
attribute station description String station where sample was taken
attribute station long_name String Station
attribute station units String unitless
variable latitude double
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 38.017617, 38.167117
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String latitude
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude double
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -121.850917, -121.597033
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String longitude
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable AOA_amoA double
attribute AOA_amoA _FillValue double NaN
attribute AOA_amoA actual_range double 72772.35, 641670.69
attribute AOA_amoA bcodmo_name String abundance
attribute AOA_amoA description String abundance of archaeal amoA genes in surface sediments
attribute AOA_amoA long_name String AOA Amo A
attribute AOA_amoA nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/B070/ (external link)
attribute AOA_amoA units String genes g -1 of wet sediment
variable AOB_amoA double
attribute AOB_amoA _FillValue double NaN
attribute AOB_amoA actual_range double 10865.6, 1026692.51
attribute AOB_amoA bcodmo_name String abundance
attribute AOB_amoA description String abundance of bacterial amoA genes in surface sediments
attribute AOB_amoA long_name String AOB Amo A
attribute AOB_amoA nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/B070/ (external link)
attribute AOB_amoA units String genes g -1 of wet sediment
variable log_AOAtoAOB float
attribute log_AOAtoAOB _FillValue float NaN
attribute log_AOAtoAOB actual_range float -1.149, 1.771
attribute log_AOAtoAOB bcodmo_name String abundance
attribute log_AOAtoAOB description String AOA gene abundances divided by AOB gene abundances, log10 transformed
attribute log_AOAtoAOB long_name String Log AOAto AOB
attribute log_AOAtoAOB nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/B070/ (external link)
attribute log_AOAtoAOB units String dimensionless
variable NO2 float
attribute NO2 _FillValue float NaN
attribute NO2 actual_range float 0.14, 0.77
attribute NO2 bcodmo_name String NO2
attribute NO2 colorBarMaximum double 1.0
attribute NO2 colorBarMinimum double 0.0
attribute NO2 description String nitrite concentrations in bottom water
attribute NO2 long_name String Mole Concentration Of Nitrite In Sea Water
attribute NO2 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/NTRIAAZX/ (external link)
attribute NO2 units String micromoles
variable NO3 float
attribute NO3 _FillValue float NaN
attribute NO3 actual_range float 1.79, 22.57
attribute NO3 bcodmo_name String NO3
attribute NO3 colorBarMaximum double 50.0
attribute NO3 colorBarMinimum double 0.0
attribute NO3 description String nitrate concentrations in bottom water
attribute NO3 long_name String Mole Concentration Of Nitrate In Sea Water
attribute NO3 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/NTRAIGGS/ (external link)
attribute NO3 units String micromoles
variable NH4 float
attribute NH4 _FillValue float NaN
attribute NH4 actual_range float 1.08, 27.12
attribute NH4 bcodmo_name String Ammonium
attribute NH4 colorBarMaximum double 5.0
attribute NH4 colorBarMinimum double 0.0
attribute NH4 description String ammonium concentrations in bottom water
attribute NH4 long_name String Mole Concentration Of Ammonium In Sea Water
attribute NH4 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/AMONAAZX/ (external link)
attribute NH4 units String micromoles
variable nitrification float
attribute nitrification _FillValue float NaN
attribute nitrification actual_range float 8.29, 146.0
attribute nitrification bcodmo_name String unknown
attribute nitrification description String potential nitrification rates in surface sediments
attribute nitrification long_name String Nitrification
attribute nitrification units String nmol NOx g-1 h-1

 
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