BCO-DMO ERDDAP
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Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Cultures:\\u00a0Pleurochrysis carterae\\u00a0cultures were maintained in\nexponential growth phase under axenic conditions in semi-continuous batch\nculture using L1-Si media prepared on 0.2 um-filtered, UV-sterilized,\nautoclaved seawater.\\u00a0 Cultures were acclimated to one of\nthree\\u00a0pCO2\\u00a0treatments for > 9 generations before experiments were\nperformed.\\u00a0 Cultures were maintained in an incubator at 16.5 +/- 0.5 deg\nC and 470 umol photons/m-2/s\\u00a0PAR.\n \npCO2: Carbonate chemistry was manipulated by bubbling cultures and prepared\nmedia with 500 mL/min\\u00a0with 0.2 um-filtered 280, 380, or 750\nppm\\u00a0pCO2\\u00a0air.\\u00a0 The\\u00a0pCO2\\u00a0levels of the treatment air\nwere established using two mass flow controllers (Aalborg, Orangeburg, NY,\nUSA) for each treatment to precisely mix in-house compressed air and pure\nCO2\\u00a0(Maine Oxy, Auburn, ME, USA).\\u00a0 The in-house compressed air was\nstripped of CO2\\u00a0to less than 10 ppm CO2\\u00a0using a Puregas VCD\nCO2\\u00a0Adsorber (Puregas, LLC, Broomfield, CO, USA).\\u00a0\nThe\\u00a0pCO2\\u00a0of the gas mixtures was stable to +/- 8\nppm.\\u00a0\\u00a0pCO2\\u00a0values of the cultures may be different than the\ntarget levels due to biological activity.\n \nDissolution of existing coccoliths:\\u00a0 Coccoliths were dissolved by 1.75 M\nHCl to drop the pH to 5.5 for 2 min.\\u00a0 Following the dissolution (de-\nlithing), 1.75 M NaOH was added to bring the pH back to the respective\nstarting pH.\\u00a0 Dissolution of coccoliths was immediately confirmed by\nlooking at the cells under cross-polarized light microscopy to verify the\nabsence of birefringence indicative of CaCO3.\\u00a0 Dissolution was further\nconfirmed by filtering the acidified/neutralized sample onto a 0.4 um\npolycarbonate filter.\\u00a0 Filters were mounted on stubs, sputter-coated with\ngold using a Denton Desk IV sputter coater (Denton Vacuum, Moorestown, NJ,\nUSA), and imaged on a Zeiss Supra25 field emission scanning electron\nmicroscope (Carl Zeiss Microscopy, LLC, Thornwood, NY, USA).\\u00a0 At least 15\ncells per sample were imaged and the number of coccoliths/cell\\u00a0was\nmanually counted to determine the number of coccoliths that remained after the\nacidification/neutralization dissolution step.\n \n24 h incubation in either light or dark conditions:\\u00a0 To determine the\nnumber of coccoliths formed (as a proxy for calcification rate) in 24 h in\neither light or dark conditions, for each\\u00a0pCO2\\u00a0level, 15 mL of de-\nlithed culture were added to 8 scintillation vials.\\u00a0 Three vials were\n\\u2018light\\u2019 replicates, three vials were \\u2018dark\\u2019 replicates,\nand two vials were poisoned with buffered formalin to serve as a\n\\u2018light\\u2019 blank and a \\u2018dark\\u2019 blank.\\u00a0 Dark replicate and\nblank vials were covered in black aluminum foil and all vials were incubated\ntogether for 24 h in an incubator set at 16.5 +/- 0.5 deg C and 470 umol\nphotons/m-2/s\\u00a0PAR on a 14-10 h light-dark cycle.\\u00a0The experiment was\ntimed to start when the lights in the incubator turned on in the morning, thus\nthe \\u2018light\\u2019 replicates were exposed to light for 14 of 24 h.\\u00a0\n \nDetermination of attached coccoliths:\\u00a0\\u00a0Coccolith formation was\nassessed by counting the number of coccoliths formed during the incubation\nperiod.\\u00a0 After the 24 h incubation period, each replicate and blank vial\nwas filtered onto a 0.4 um polycarbonate filters.\\u00a0 Filters were mounted\non stubs, sputter-coated with gold using a Denton Desk IV sputter coater\n(Denton Vacuum, Moorestown, NJ, USA), and imaged on a Zeiss Supra25 field\nemission SEM (Carl Zeiss Microscopy, LLC, Thornwood, NY, USA).\\u00a0 At least\n15 cells per replicate were imaged and the number of coccoliths/cell\\u00a0was\nmanually counted.\\u00a0 The counted coccoliths represented calcification\nduring the 24 h incubation period and the average number of coccoliths per\ncell for each replicate and blank is reported.
attribute NC_GLOBAL awards_0_award_nid String 514411
attribute NC_GLOBAL awards_0_award_number String OCE-1220068
attribute NC_GLOBAL awards_0_data_url String http://nsf.gov/awardsearch/showAward?AWD_ID=1220068 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Light-Dark Calcification \n  W. Balch and D. Fields, PIs \n  Version 4 November 2016
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3  19 Dec 2019
attribute NC_GLOBAL date_created String 2016-11-04T21:25:51Z
attribute NC_GLOBAL date_modified String 2019-04-18T15:55:03Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.664013.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/664013 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String in-situ incubator
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Dark replicate and blank vials were covered in black aluminum foil and all vials were incubated together for 24 h in an incubator set at 16.5 +/- 0.5 deg C and 470 umol photons/m-2/s PAR on a 14-10 h light-dark cycle.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 664040
attribute NC_GLOBAL instruments_0_description String A device on shipboard or in the laboratory that holds water samples under controlled conditions of temperature and possibly illumination.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/82/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String In-situ incubator
attribute NC_GLOBAL instruments_0_instrument_nid String 494
attribute NC_GLOBAL instruments_0_supplied_name String Incubator
attribute NC_GLOBAL instruments_1_acronym String pH Sensor
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Orion ROSS electrode was connected to an Orion Star A211 Benchtop pH meter (ThermoFisher Scientific, Waltham, MA, USA)
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 664025
attribute NC_GLOBAL instruments_1_description String General term for an instrument that measures the pH or how acidic or basic a solution is.
attribute NC_GLOBAL instruments_1_instrument_name String pH Sensor
attribute NC_GLOBAL instruments_1_instrument_nid String 674
attribute NC_GLOBAL instruments_1_supplied_name String Orion ROSS electrode
attribute NC_GLOBAL instruments_2_dataset_instrument_description String Carl Zeiss Microscopy, LLC, Thornwood, NY, USA
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 664039
attribute NC_GLOBAL instruments_2_description String Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of electrons behaving as waves.
attribute NC_GLOBAL instruments_2_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB07/ (external link)
attribute NC_GLOBAL instruments_2_instrument_name String Microscope-Electron
attribute NC_GLOBAL instruments_2_instrument_nid String 709
attribute NC_GLOBAL instruments_2_supplied_name String Zeiss Supra25 field emission SEM
attribute NC_GLOBAL instruments_3_acronym String MFC
attribute NC_GLOBAL instruments_3_dataset_instrument_description String Indicate and control set flow rates of gases. Manufactured in Orangeburg, NY USA.
attribute NC_GLOBAL instruments_3_dataset_instrument_nid String 664022
attribute NC_GLOBAL instruments_3_description String Mass Flow Controller (MFC) - A device used to measure and control the flow of fluids and gases
attribute NC_GLOBAL instruments_3_instrument_name String Mass Flow Controller
attribute NC_GLOBAL instruments_3_instrument_nid String 712
attribute NC_GLOBAL instruments_3_supplied_name String Aalborg Mass Flow Controller
attribute NC_GLOBAL instruments_4_acronym String CO2 Adsorber
attribute NC_GLOBAL instruments_4_dataset_instrument_description String Instrument stripped compressed air of CO2
attribute NC_GLOBAL instruments_4_dataset_instrument_nid String 664023
attribute NC_GLOBAL instruments_4_description String CO2 Adsorber - an instrument designed to remove CO2 and moisture from compressed air.
attribute NC_GLOBAL instruments_4_instrument_name String CO2 Adsorber
attribute NC_GLOBAL instruments_4_instrument_nid String 651526
attribute NC_GLOBAL instruments_4_supplied_name String Puregas VCD CO2 Adsorber
attribute NC_GLOBAL instruments_5_acronym String Sputter Coater
attribute NC_GLOBAL instruments_5_dataset_instrument_description String Filters were mounted on stubs, sputter-coated with gold using a Denton Desk IV sputter coater (Denton Vacuum, Moorestown, NJ, USA).
attribute NC_GLOBAL instruments_5_dataset_instrument_nid String 664042
attribute NC_GLOBAL instruments_5_description String Sputter coating is the standard method for preparing non-conducting or poorly conducting specimens prior to observation in a scanning electron microscope (SEM)
attribute NC_GLOBAL instruments_5_instrument_name String Sputter Coater
attribute NC_GLOBAL instruments_5_instrument_nid String 664041
attribute NC_GLOBAL instruments_5_supplied_name String Denton Desk IV sputter coater
attribute NC_GLOBAL keywords String attached, attached_blankCorrected, bco, bco-dmo, biological, blank, carbon, carbon dioxide, chemical, co2, conditions, corrected, data, dataset, deviation, dioxide, dmo, erddap, light, light_conditions, management, mean, mean_attached_blankCorrected, oceanography, office, pCO2_treatment, preliminary, sample, standard, standard deviation, stdev, stdev_attached_blankCorrected, treatment
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/664013/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/664013 (external link)
attribute NC_GLOBAL param_mapping String {'664013': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/664013/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Bigelow Laboratory for Ocean Sciences
attribute NC_GLOBAL people_0_person_name String William M. Balch
attribute NC_GLOBAL people_0_person_nid String 50650
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Bigelow Laboratory for Ocean Sciences
attribute NC_GLOBAL people_1_person_name String David Fields
attribute NC_GLOBAL people_1_person_nid String 51141
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Bigelow Laboratory for Ocean Sciences
attribute NC_GLOBAL people_2_person_name String William M. Balch
attribute NC_GLOBAL people_2_person_nid String 50650
attribute NC_GLOBAL people_2_role String Contact
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL people_3_affiliation String Bigelow Laboratory for Ocean Sciences
attribute NC_GLOBAL people_3_person_name String Meredith White
attribute NC_GLOBAL people_3_person_nid String 514420
attribute NC_GLOBAL people_3_role String Contact
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL people_4_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_4_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_4_person_name String Hannah Ake
attribute NC_GLOBAL people_4_person_nid String 650173
attribute NC_GLOBAL people_4_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_4_role_type String related
attribute NC_GLOBAL project String OA_Copes_Coccoliths
attribute NC_GLOBAL projects_0_acronym String OA_Copes_Coccoliths
attribute NC_GLOBAL projects_0_description String (Extracted from the NSF award abstract)\nOcean acidification is one of the most pressing marine science issues of our time, with potential biological impacts spanning all marine phyla and potential societal impacts affecting man's relationship to the sea. Rising levels of atmospheric pCO2 are increasing the acidity of the world oceans. It is generally held that average surface ocean pH has already declined by 0.1 pH units relative to the pre-industrial level (Orr et al., 2005), and is projected to decrease 0.3 to 0.46 units by the end of this century, depending on CO2 emission scenarios (Caldeira and Wickett, 2005). The overall goal of this research is to parameterize how changes in pCO2 levels could alter the biological and alkalinity pumps of the world ocean. Specifically, the direct and indirect effects of ocean acidification will be examined within a simple, controlled predator/prey system containing a single prey phytoplankton species (the coccolithophore, Emiliania huxleyi) and a single predator (the oceanic metazoan grazer, Calanus finmarchicus). The experiments are designed to elucidate both direct effects (i.e. effects of ocean acidification on the individual organisms only) and interactive effects (i.e. effects on the combined predator/prey system). Interactive experiments with phytoplankton prey and zooplankton predator are a critical starting point for predicting the overall impact of ocean acidification in marine ecosystems. To meet these goals, a state-of-the-art facility will be constructed with growth chambers that are calibrated and have highly-controlled pH and alkalinity levels. The strength of this approach lies in meticulous calibration and redundant measurements that will be made to ensure that conditions within the chambers are well described and tightly monitored for DIC levels. Growth and calcification rates in coccolithophores and the developmental rates, morphological and behavioral effects on copepods will be measured. The PIC and POC in the algae and the excreted fecal pellets will be monitored for changes in the PIC/POC ratio, a key parameter for modeling feedback mechanisms for rising pCO2 levels. In addition, 14C experiments are planned to measure calcification rates in coccolithophores and dissolution rates as a result of grazing. These key experiments will verify closure in the mass balance of PIC, allowing the determination of actual dissolution rates of PIC within the guts of copepod grazers.
attribute NC_GLOBAL projects_0_end_date String 2015-07
attribute NC_GLOBAL projects_0_geolocation String Laboratory experiments;  East Boothbay, Maine
attribute NC_GLOBAL projects_0_name String Effects of ocean acidification on Emiliania huxleyi and Calanus finmarchicus; insights into the oceanic alkalinity and biological carbon pumps
attribute NC_GLOBAL projects_0_project_nid String 514415
attribute NC_GLOBAL projects_0_start_date String 2012-08
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Light-dark calcification rates of Pleurochrysis carterae analyzed at Bigelow Laboratory in 2013 (OA Copes Coccoliths project)
attribute NC_GLOBAL title String [Pleurochrysis carterae light-dark calcification] - Light-dark calcification rates of Pleurochrysis carterae analyzed at Bigelow Laboratory in 2013 (OA Copes Coccoliths project) (Effects of ocean acidification on Emiliania huxleyi and Calanus finmarchicus; insights into the oceanic alkalinity and biological carbon pumps)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable pCO2_treatment short
attribute pCO2_treatment _FillValue short 32767
attribute pCO2_treatment actual_range short 280, 750
attribute pCO2_treatment bcodmo_name String treatment
attribute pCO2_treatment description String The independent variable - one of three pCO2 levels (280 ppm, 380 ppm, or 750 ppm) These treatment levels are nominal values as they represent the target pCO2 for each treatment.
attribute pCO2_treatment long_name String P CO2 Treatment
attribute pCO2_treatment units String parts per million (ppm)
variable sample String
attribute sample bcodmo_name String sample
attribute sample description String For each pCO2 treatment there are three types of samples;\nAfter de-lithing: These samples were taken immediately after the coccolithophores were acidified to dissolve their coccoliths and then neutralized to return the culture pH to the original pH.  These samples were taken before the 24 h incubation period and therefore do not have a Light or Dark treatment.\nReplicate: These represent the three replicates for each light condition and each CO2 treatment.  These samples were taken after the cells incubated in either light or dark conditions for 24 h.\nBlank:  These samples were killed by the addition of formalin but were allowed to incubate in either light or dark conditions for 24 h.
attribute sample long_name String Sample
attribute sample nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute sample units String unitless
variable light_conditions String
attribute light_conditions bcodmo_name String PAR
attribute light_conditions description String This identifies whether the sample was incubated for 24 h in light (470 umol photons/m-2/s PAR) or dark (0 umol photons/m-2/s PAR) conditions.
attribute light_conditions long_name String Light Conditions
attribute light_conditions units String unitless
variable attached float
attribute attached _FillValue float NaN
attribute attached actual_range float 0.0, 33.0
attribute attached bcodmo_name String cell_concentration
attribute attached description String This is the number of attached coccoliths observed per cell.  This number represents an average of counts from at least 15 cells per replicate or sample.
attribute attached long_name String Attached
attribute attached units String coccoliths per cell
variable attached_blankCorrected float
attribute attached_blankCorrected _FillValue float NaN
attribute attached_blankCorrected actual_range float 0.0, 32.8
attribute attached_blankCorrected bcodmo_name String cell_concentration
attribute attached_blankCorrected description String This is the number of attached coccoliths observed per cell with the number of coccoliths observed per cell from the blank samples subtracted to account for any coccoliths that might have remained after cells were de-lithed.
attribute attached_blankCorrected long_name String Attached Blank Corrected
attribute attached_blankCorrected units String coccoliths per cell
variable mean_attached_blankCorrected float
attribute mean_attached_blankCorrected _FillValue float NaN
attribute mean_attached_blankCorrected actual_range float 0.21, 30.72
attribute mean_attached_blankCorrected bcodmo_name String mean
attribute mean_attached_blankCorrected description String The average value for each set of three replicates.
attribute mean_attached_blankCorrected long_name String Mean Attached Blank Corrected
attribute mean_attached_blankCorrected units String coccoliths per cell
variable stdev_attached_blankCorrected float
attribute stdev_attached_blankCorrected _FillValue float NaN
attribute stdev_attached_blankCorrected actual_range float 0.37, 2.75
attribute stdev_attached_blankCorrected bcodmo_name String standard deviation
attribute stdev_attached_blankCorrected description String The standard deviation for each set of three replicates.
attribute stdev_attached_blankCorrected long_name String Stdev Attached Blank Corrected
attribute stdev_attached_blankCorrected units String coccoliths per cell

 
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