BCO-DMO ERDDAP
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Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Using a small boat, samples were collected in 20L carboys in Tylerfjord-Young\nSound. Three rivers that feed into Tyrolerfjord-Young Sound (Tyroler River,\nLerbugten River and Zackenberg River) were sampled; surface and subsurface\nwater samples were also collected at transition sites where the rivers feed\ninto the fjord (Tyro_01, Zac_30, Ler_30, altogether referred to as \\u2018river\ntransition sites\\u2019). Enzyme activities were measured in unfiltered water.\nIn addition, water was size-fractionated using gravity filtration through a\nGF/A filter to capture \\u22651.6 \\u00b5m particles.\n \nTwo substrates, a-glucose and b-glucose linked to a 4-methylumbelliferyl (MUF)\nfluorophore, were used to measure glucosidase activities. Five substrates\nlinked to a 7-amido-4-methyl coumarin (MCA) fluorophore, one amino acid \\u2013\nleucine \\u2013 and four oligopeptides \\u2013 the chymotrypsin substrates\nalanine-alanine-phenylalanine (AAF) and alanine-alanine-proline-phenylalanine\n(AAPF), and the trypsin substrates glutamine-alanine-arginine (QAR) and\nphenylalanine-serine-arginine (FSR) \\u2013 were used to measure exo- and endo-\nacting peptidase activities, respectively. Hydrolysis rates of the substrates\nwere measured as an increase in fluorescence as the fluorophore was hydrolyzed\nfrom the substrate over time [as in Hoppe, 1993; Obayashi and Suzuki, 2005].\n\\u00a0All substrates were used to measure enzyme activities in unfiltered\nwater, as well as particle-associated (\\u22651.6 \\u00b5m) enzymatic\nactivities.\n \nIn unfiltered water, enzyme activities were measured by adding 4 mL of water\nto triplicate cuvettes. One incubation containing autoclaved water served as\nthe killed control. This procedure was applied to each of the 7 substrates and\none live blank and autoclave blank (no substrate addition). Each cuvette\ncontaining either live or autoclaved water was amended with one substrate to a\nconcentration of 100 \\u00b5M. Fluroescence was measured using a Promega\nQuanti\\ufb02uor solid-state single-cuvette \\ufb02uorimeter; excitation and\nemission maxima were 365 nm and 410\\u2013450 nm, respectively,\n \nTo measure particle-associated enzyme assays, 1/12th piece of a GF/A filter\nthrough which water had been gravity filtered was put into a cuvette\ncontaining 4 mL of cooled, autoclaved water from the same station/depth as the\nlive samples. In addition, killed controls were set up using sterile GF/A\nfilters cut into 1/12th pieces. Bulk water and particle-associated enzyme\nassays were incubated for up to 24 and 16 hours, respectively; timepoints were\ntaken at specific intervals. Incubations were kept in the dark either at\n0\\u00b0C, 5\\u00b0C, or 8\\u00b0C, depending on in situ water temperature at the\ntime of sampling.\n \nActivities of polysaccharide hydrolases were measured using fluorescently\nlabeled polysaccharides (Arnosti 2003). Activities of enzymes that hydrolyze\npullulan, laminarin, xylan, fucoidan, arabinogalactan, and chondroitin sulfate\nwere measured in unfiltered water, and using GF/A filters through which water\nhad been gravity-filtered. For these measurements, substrate was added (of 3.5\n\\u00b5M monomer equivalent) to 15 mL of water; autoclaved ambient water served\nas the killed control. Particle-associated activities were measured by\nsubmerging 1/12th of a GF/A filter in 15 ml autoclaved seawater. Samples were\nincubated in the dark at near in situ temperature (0\\u00b0C, 5\\u00b0C, or\n8\\u00b0C), and sub-sampled at specific time intervals\\u2014t0 (0h, upon\nsubstrate addition), t1 (120 h), t2 (240 h), t3 (360 h) and t4 (600 h). Sub-\nsamples from each timepoint were filtered using 0.2 \\u00b5M pore size SFCA\n(surfactant-free cellulose acetate) syringe filters, and the filtrate was\ncollected in tubes and frozen at -20\\u00b0C until processing in the lab. Sub-\nsamples were processed using gel permeation chromatography (Arnosti,\n2003).\\u00a0
attribute NC_GLOBAL awards_0_award_nid String 712358
attribute NC_GLOBAL awards_0_award_number String OCE-1332881
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1332881 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Henrietta N Edmonds
attribute NC_GLOBAL awards_0_program_manager_nid String 51517
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Microbial enzyme activities and bacterial productivity: hydrolysis rates \n   Greenland bulk (unfiltered) and GF filtered samples \n   C. Arnosti (UNC) \n   version: 2017-10-30
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3  19 Dec 2019
attribute NC_GLOBAL dataset_current_state String Final and no updates
attribute NC_GLOBAL date_created String 2017-10-25T16:47:01Z
attribute NC_GLOBAL date_modified String 2020-05-13T14:07:45Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.717660.1
attribute NC_GLOBAL geospatial_vertical_max double 20.0
attribute NC_GLOBAL geospatial_vertical_min double 1.0
attribute NC_GLOBAL geospatial_vertical_positive String down
attribute NC_GLOBAL geospatial_vertical_units String m
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/717660 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Niskin bottle
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to collect water for large volume mesocosm experiments
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 717666
attribute NC_GLOBAL instruments_0_description String A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Niskin bottle
attribute NC_GLOBAL instruments_0_instrument_nid String 413
attribute NC_GLOBAL instruments_0_supplied_name String 20 liter Niskin bottles
attribute NC_GLOBAL instruments_1_acronym String Fluorometer
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 718104
attribute NC_GLOBAL instruments_1_description String A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/113/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Fluorometer
attribute NC_GLOBAL instruments_1_instrument_nid String 484
attribute NC_GLOBAL instruments_1_supplied_name String Promega Quantifluor solid-state single-cuvette fluorimeter
attribute NC_GLOBAL keywords String average, bco, bco-dmo, biological, cast, chemical, data, dataset, depth, depth_id, depth_m, dev, dmo, elapsed, erddap, filter, filter_um, fluorophore, management, oceanography, office, preliminary, profiler, rate, rate_average, rate_std_dev, rep1, rep1_rate, rep2, rep2_rate, rep3, rep3_rate, salinity, salinity-temperature-depth, sample, sample_type, station, std, substrate, temperature, time, time_elapsed_hr, timepoint, trip, trip_id, type
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/717660/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/717660 (external link)
attribute NC_GLOBAL param_mapping String {'717660': {'depth_m': 'master - depth'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/717660/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of North Carolina at Chapel Hill
attribute NC_GLOBAL people_0_affiliation_acronym String UNC-Chapel Hill
attribute NC_GLOBAL people_0_person_name String Carol Arnosti
attribute NC_GLOBAL people_0_person_nid String 661940
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_1_person_name String Nancy Copley
attribute NC_GLOBAL people_1_person_nid String 50396
attribute NC_GLOBAL people_1_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL project String Patterns of activities
attribute NC_GLOBAL projects_0_acronym String Patterns of activities
attribute NC_GLOBAL projects_0_description String NSF Award Abstract:\nHeterotrophic microbial communities are key players in the marine carbon cycle, transforming and respiring organic carbon, regenerating nutrients, and acting as the final filter in sediments through which organic matter passes before long-term burial. Microbially-driven carbon cycling in the ocean profoundly affects the global carbon cycle, but key factors determining rates and locations of organic matter remineralization are unclear. In this study, researchers from the University of North Carolina at Chapel Hill will investigate the ability of pelagic microbial communities to initiate the remineralization of polysaccharides and proteins, which together constitute a major pool of organic matter in the ocean. Results from this study will be predictive on a large scale regarding the nature of the microbial response to organic matter input, and will provide a mechanistic framework for interpreting organic matter reactivity in the ocean.\nBroader Impacts: This study will provide scientific training for undergraduate and graduate students from underrepresented groups. The project will also involve German colleagues, thus strengthening international scientific collaboration.
attribute NC_GLOBAL projects_0_end_date String 2017-07
attribute NC_GLOBAL projects_0_geolocation String Atlantic Ocean, Arctic Ocean, Pacific Ocean, Greenland
attribute NC_GLOBAL projects_0_name String Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean?
attribute NC_GLOBAL projects_0_project_nid String 712359
attribute NC_GLOBAL projects_0_start_date String 2013-08
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL subsetVariables String trip_id
attribute NC_GLOBAL summary String Bacterial activity as measured by hydrolysis rates from unfiltered seawater and particle-associated communities collected near shore in northeastern Greenland in August 2015.
attribute NC_GLOBAL title String [Greenland experiment: microbial enzymatic activities] - Microbial enzymatic activities from seawater and from particle-associated seawater communities from Greenland, August 2015 (Patterns of activities project) (Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean?)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.5
variable trip_id String
attribute trip_id bcodmo_name String Cruise Name
attribute trip_id description String sampling trip identifier; YS means Young Sound
attribute trip_id long_name String Trip Id
attribute trip_id units String unitless
variable sample_type String
attribute sample_type bcodmo_name String sample_type
attribute sample_type description String indication of whether sample was filtered (GF) or not (bulk)
attribute sample_type long_name String Sample Type
attribute sample_type units String unitless
variable filter_um float
attribute filter_um _FillValue float NaN
attribute filter_um actual_range float 1.6, 1.6
attribute filter_um bcodmo_name String filter_size
attribute filter_um description String filter size
attribute filter_um long_name String Filter Um
attribute filter_um units String nanomol monomer/liter/hour
variable fluorophore String
attribute fluorophore bcodmo_name String unknown
attribute fluorophore description String fluorescent molecules used to measure hydrolysis rates:  fluorescently-labeled polysaccharides (FLA) or small substrate proxies tagged with methylcoumarine (MCA) and methylumbelliferone (MUF) fluorophores.
attribute fluorophore long_name String Fluorophore
attribute fluorophore units String unitless
variable station String
attribute station bcodmo_name String station
attribute station description String station identifier
attribute station long_name String Station
attribute station units String unitless
variable cast int
attribute cast _FillValue int 2147483647
attribute cast actual_range int 120808, 171521
attribute cast bcodmo_name String cast
attribute cast description String cast identifier
attribute cast long_name String Cast
attribute cast units String unitless
variable depth_id String
attribute depth_id bcodmo_name String depth_comment
attribute depth_id description String depth description: sequence of depths sampled with 1 is surface and higher numbers at greater depths
attribute depth_id long_name String Depth
attribute depth_id standard_name String depth
attribute depth_id units String unitless
variable depth double
attribute depth _CoordinateAxisType String Height
attribute depth _CoordinateZisPositive String down
attribute depth _FillValue double NaN
attribute depth actual_range double 1.0, 20.0
attribute depth axis String Z
attribute depth bcodmo_name String depth
attribute depth colorBarMaximum double 8000.0
attribute depth colorBarMinimum double -8000.0
attribute depth colorBarPalette String TopographyDepth
attribute depth description String actual depth at which water collected
attribute depth ioos_category String Location
attribute depth long_name String Depth
attribute depth nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/DEPH/ (external link)
attribute depth positive String down
attribute depth standard_name String depth
attribute depth units String m
variable substrate String
attribute substrate bcodmo_name String unknown
attribute substrate description String substrates for measurement of enzymatic activities: ara = arabinogalactan; chn = chondroitin sulfate; fuc = fucoidan; lam = laminarin; pul = pullulan; xyl = xylan
attribute substrate long_name String Substrate
attribute substrate units String unitless
variable timepoint String
attribute timepoint bcodmo_name String time_point
attribute timepoint description String sampling time point (0; 1; 2; etc.) post-incubation
attribute timepoint long_name String Timepoint
attribute timepoint units String unitless
variable time_elapsed_hr short
attribute time_elapsed_hr _FillValue short 32767
attribute time_elapsed_hr actual_range short 0, 982
attribute time_elapsed_hr bcodmo_name String time_elapsed
attribute time_elapsed_hr description String hours elapsed to reach a specific timepoint
attribute time_elapsed_hr long_name String Time Elapsed Hr
attribute time_elapsed_hr nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/ (external link)
attribute time_elapsed_hr units String hours
variable rep1_rate float
attribute rep1_rate _FillValue float NaN
attribute rep1_rate actual_range float 0.0, 76.82
attribute rep1_rate bcodmo_name String unknown
attribute rep1_rate description String replicate 1 hydrolysis rate
attribute rep1_rate long_name String Rep1 Rate
attribute rep1_rate units String nanomol monomer/liter/hour
variable rep2_rate float
attribute rep2_rate _FillValue float NaN
attribute rep2_rate actual_range float 0.0, 77.36
attribute rep2_rate bcodmo_name String unknown
attribute rep2_rate description String replicate 2 hydrolysis rate
attribute rep2_rate long_name String Rep2 Rate
attribute rep2_rate units String nanomol monomer/liter/hour
variable rep3_rate float
attribute rep3_rate _FillValue float NaN
attribute rep3_rate actual_range float 0.0, 91.25
attribute rep3_rate bcodmo_name String unknown
attribute rep3_rate description String replicate 3 hydrolysis rate
attribute rep3_rate long_name String Rep3 Rate
attribute rep3_rate units String nanomol monomer/liter/hour
variable rate_average float
attribute rate_average _FillValue float NaN
attribute rate_average actual_range float 0.0, 81.81
attribute rate_average bcodmo_name String unknown
attribute rate_average description String average of the 3 hydrolysis rates
attribute rate_average long_name String Rate Average
attribute rate_average units String nanomol monomer/liter/hour
variable rate_std_dev String
attribute rate_std_dev bcodmo_name String unknown
attribute rate_std_dev description String standard deviation of the 3 hydrolysis rates
attribute rate_std_dev long_name String Rate Std Dev
attribute rate_std_dev units String nanomol monomer/liter/hour

 
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