BCO-DMO ERDDAP
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Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Irradiance exposure experiments:\\u00a0 Experiments to test the light stress\nresponse consisted of exposing dinoflagellates to natural sunlight and\nmonitoring a range of physiological and biochemical responses. Sunlight\nexposure took place in unshaded outdoor Plexiglas tanks (without lids),\nsupplied with a constant flow of seawater to maintain incubation bottles\napproximate growth temperatures of 15-16\\u00b0C for the duration of each\nexperimental exposure period. A Li-Cor LI-1400 data logger with a 2\\u03c0\nsensor collected incident PAR data; irradiance received during incubation\nperiod is presented as total dose (mol photons m-2) and (for Fv/Fm data) as\nthe maximum instantaneous irradiance recorded during the exposure period\n(\\u00b5mol photons m-2 s-1). To initiate experiments, dinoflagellate cultures\nwere combined and divided into 250-mL polycarbonate bottles, which block\npenetration of UVR. Bottles were returned to the growth incubator for\napproximately 1 h acclimation before the experiment began. After acclimation,\n\\u201cpre-exposure\\u201d samples were collected from each bottle for\ndetermination of morphological, biochemical, and physiological characteristics\nof dinoflagellate cells. Bottles were then covered in treatment-specific\nneutral density screening as needed to achieve desired irradiances, and placed\nin the outdoor tank to begin the light exposure period. Control bottles were\ncovered with enough screen layers to approximate growth incubator irradiances.\nExperiments included four replicates each of either two or three treatments:\nall experiments included \\u201chigh PAR\\u201d and \\u201ccontrol\\u201d\ntreatments, while A2 and H2 also included \\u201cmoderate PAR\\u201d. Exposure\nduration varied slightly by experiment but was approximately 1.5 h.\nPhotosynthetic efficiency Fv/Fm (unitless) was measured at 15, 25, or 30 min\ntime intervals during the exposure period, depending on the experiment. After\nthe exposure period, all bottles were taken indoors for a second round of\nsampling (henceforth referred to as \\u201cpost-exposure\\u201d). Bottles were\nthen rid of any screening and returned to the growth incubator for the\nrecovery period (1.5-2 h depending on experiment). After recovery a third\nround of samples (termed \\u201cpost-recovery\\u201d) was collected.\n \nPhotosynthetic efficiency Fv/Fm:\\u00a0 Samples (2 mL) were taken from each\nbottle at regular intervals (see above) during the light treatment exposure\nand subsequent recovery period; samples were then dark-incubated at 15\\u00b0C\nfor 20 min. After dark acclimation, Fv/Fm was measured using a Walz Water-PAM\npulse amplitude-modulated fluorometer.
attribute NC_GLOBAL awards_0_award_nid String 614837
attribute NC_GLOBAL awards_0_award_number String OCE-1434842
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1434842 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Irradiance exposure experiments \n  S. Strom, PI \n  Version 22 January 2018
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3  19 Dec 2019
attribute NC_GLOBAL date_created String 2018-01-11T20:04:43Z
attribute NC_GLOBAL date_modified String 2019-03-18T18:42:30Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.723266.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/723266 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Fluorometer
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to measure FvFm
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 723341
attribute NC_GLOBAL instruments_0_description String A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/113/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Fluorometer
attribute NC_GLOBAL instruments_0_instrument_nid String 484
attribute NC_GLOBAL instruments_0_supplied_name String Walz Water-PAM pulse amplitude-modulated fluorometer
attribute NC_GLOBAL instruments_1_acronym String LI-COR LI-1000
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Used to collect PAR data
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 723340
attribute NC_GLOBAL instruments_1_description String \"The LI-1000 DataLogger is a 10 channel datalogger that functions both as a data logging device and a multichannel, autoranging meter.  The electronics of the LI-1000 have been optimized for highly accurate measurement of LI-COR radiation sensors which have a current signal\" (from LI-COR Datalogging Instruction Manual, p 1).  LI-COR began manufacturing these instruments in 1985 and discontinued in 1998.  Serial Numbers for this model have the prefix of LDL-XXXX. (www.licor.com)
attribute NC_GLOBAL instruments_1_instrument_name String LI-COR LI-1000 Data Logger
attribute NC_GLOBAL instruments_1_instrument_nid String 543
attribute NC_GLOBAL instruments_1_supplied_name String Li-Cor LI-1400 data logger with a 2π sensor
attribute NC_GLOBAL keywords String available, bco, bco-dmo, biological, chemical, data, dataset, date, dinoflagellate, Dinoflagellate_species, dmo, erddap, experiment, Experiment_Date, Experiment_ID, FvFm, iso, management, max, Max_PAR, number, oceanography, office, par, photosynthetically, preliminary, radiation, sample, Sample_Number, sampling, Sampling_Time, species, time, total, Total_PAR, treatment
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/723266/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/723266 (external link)
attribute NC_GLOBAL param_mapping String {'723266': {'ISO_DateTime_UTC': 'flag - time'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/723266/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Western Washington University - Shannon Point Marine Center
attribute NC_GLOBAL people_0_affiliation_acronym String SPMC
attribute NC_GLOBAL people_0_person_name String Suzanne Strom
attribute NC_GLOBAL people_0_person_nid String 50471
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_1_person_name String Hannah Ake
attribute NC_GLOBAL people_1_person_nid String 650173
attribute NC_GLOBAL people_1_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL project String Protist signaling
attribute NC_GLOBAL projects_0_acronym String Protist signaling
attribute NC_GLOBAL projects_0_description String Description from NSF proposal:\nThis proposal arises from the central premise that the oxidative stress response is an emergent property of phototrophic cellular systems, with implications for nearly every aspect of a phytoplankton cell’s life in the upper ocean. Oxidative stress (OS) arises from the uncompensated production of reactive oxygen species (ROS) within a cell, which can occur in response to a myriad of environmental stressors (e.g. nutrient limitation, temperature extremes, toxins, variable light exposure). In addition to the biochemical damage and physiological impairment that OS can cause, the phytoplankton OS response also includes increased net production and extracellular release of ROS, osmolytes, and other compounds that are known or suspected to be potent signals regulating protist behavior. We hypothesize that, through chemical signaling, oxidative stress acts to govern relationships among environmental variability, phytoplankton condition, and protist predation. Our proposed study of these integrated signaling and response processes has three overarching objectives: 1) Create and characterize oxidatively stressed phytoplankton. We will use light stress (variable exposure to visible light and UV) to create oxidatively stressed phytoplankton in the laboratory. Common coastal taxa with contrasting stress responses will be characterized using an array of fluorescent probes, biochemical measurements, and physiological assays. In addition, intracellular production and extracellular release of ROS and the associated chemical signal DMSP will be quantified. Use of Phaeodactylum tricornutum light stress mutants will add an independent means of connecting OS to signal production and predation response. 2) Examine protist predator responses to oxidatively stressed phytoplankton and associated chemical signals. Responses will be investigated by means of manipulation experiments and thorough characterization of associated signal chemistry. Assessment of predator response will be via predation rate measurements and population aggregation/dispersal behaviors in structured columns. 3) Investigate the prevalence of OS, its environmental correlates, and the microzooplankton predation response in the natural waters of a well-characterized local embayment. Application of ROS probes and OS assays to the natural environment and the design of OS manipulation experiments will be informed by the laboratory experiments using local protist species.\nOur work will help to elucidate some of the multiple ways in which the OS response can affect phytoplankton fitness, contributing information that can be used to characterize the position of key coastal species along an OS response spectrum. Ultimately such information could be used in trait-based conceptual and numerical models in a manner analogous to cell size and other 'master traits'. Our research will also inform the relatively new and exciting field of chemical signaling in planktonic communities, exploring DMSP- and ROS-based signaling between two of the most significant groups in the plankton, the eukaryotic phytoplankton and their protist predators. Finally, findings will help elucidate the links between environmental stress, phytoplankton response, and predation in planktonic ecosystems. These links relate to central issues in biological oceanography, including the predator-prey interactions that influence bloom demise, and the mechanisms by which protists feed selectively and thereby structure prey communities. The proposed research is a cross-cutting endeavor that unites subjects usually studied in isolation through a novel conceptual framework. Thus the findings have the potential to generate broadly applicable new insights into the ecological and evolutionary regulation of this key trophic link in planktonic food webs.
attribute NC_GLOBAL projects_0_end_date String 2017-08
attribute NC_GLOBAL projects_0_geolocation String Salish Sea: 48.5, -122.75
attribute NC_GLOBAL projects_0_name String Environmental stress and signaling based on reactive oxygen species among planktonic protists
attribute NC_GLOBAL projects_0_project_nid String 614838
attribute NC_GLOBAL projects_0_start_date String 2014-09
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Photophysiological responses of two dinoflagellate species used in natural high light exposure experiments (Protist Signaling project)
attribute NC_GLOBAL time_coverage_end String 2015-09-10T19:39:00Z
attribute NC_GLOBAL time_coverage_start String 2015-07-15T12:09:00Z
attribute NC_GLOBAL title String [Irradiance exposure experiments] - Photophysiological responses of two dinoflagellate species used in natural high light exposure experiments (Protist Signaling project) (Environmental stress and signaling based on reactive oxygen species among planktonic protists)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable Experiment_ID String
attribute Experiment_ID bcodmo_name String exp_id
attribute Experiment_ID description String Experiment ID number
attribute Experiment_ID long_name String Experiment ID
attribute Experiment_ID units String unitless
variable Experiment_Date String
attribute Experiment_Date bcodmo_name String date
attribute Experiment_Date description String Experiment date; YYYY/MM/DD
attribute Experiment_Date long_name String Experiment Date
attribute Experiment_Date nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/ (external link)
attribute Experiment_Date time_precision String 1970-01-01
attribute Experiment_Date units String unitless
variable Dinoflagellate_species String
attribute Dinoflagellate_species bcodmo_name String species
attribute Dinoflagellate_species description String Species of sample
attribute Dinoflagellate_species long_name String Dinoflagellate Species
attribute Dinoflagellate_species units String unitless
variable Max_PAR float
attribute Max_PAR _FillValue float NaN
attribute Max_PAR actual_range float 0.7845, 1607.0
attribute Max_PAR bcodmo_name String PAR
attribute Max_PAR description String Maximum instantaneous PAR received during light exposure treatment
attribute Max_PAR long_name String Max PAR
attribute Max_PAR units String umol photons m^-2 s^-1
variable Total_PAR float
attribute Total_PAR _FillValue float NaN
attribute Total_PAR actual_range float 0.004833, 9.897
attribute Total_PAR bcodmo_name String PAR
attribute Total_PAR description String Total dose of photosynthetically active radiation received during lightexposure treatment
attribute Total_PAR long_name String Total PAR
attribute Total_PAR units String mol photons m^-2
variable Sampling_Time String
attribute Sampling_Time bcodmo_name String time
attribute Sampling_Time description String Time of sampling; HH:MM
attribute Sampling_Time long_name String Sampling Time
attribute Sampling_Time nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/AHMSAA01/ (external link)
attribute Sampling_Time units String unitless
variable Treatment String
attribute Treatment bcodmo_name String treatment
attribute Treatment description String Treatment
attribute Treatment long_name String Treatment
attribute Treatment units String unitless
variable Sample_Number byte
attribute Sample_Number _FillValue byte 127
attribute Sample_Number actual_range byte 1, 4
attribute Sample_Number bcodmo_name String sample
attribute Sample_Number colorBarMaximum double 100.0
attribute Sample_Number colorBarMinimum double 0.0
attribute Sample_Number description String Sample ID Number
attribute Sample_Number long_name String Sample Number
attribute Sample_Number nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute Sample_Number units String unitless
variable FvFm float
attribute FvFm _FillValue float NaN
attribute FvFm actual_range float 0.0, 0.76
attribute FvFm bcodmo_name String Fv2Fm
attribute FvFm description String Photosynthetic efficiency (variable fluorescence normalized to maximum fluorescence)
attribute FvFm long_name String FV FM
attribute FvFm units String unitless
variable time double
attribute time _CoordinateAxisType String Time
attribute time actual_range double 1.43696214E9, 1.44191394E9
attribute time axis String T
attribute time bcodmo_name String ISO_DateTime_UTC
attribute time description String DateTime ISO Formatted; UTC
attribute time ioos_category String Time
attribute time long_name String ISO Date Time UTC
attribute time nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/DTUT8601/ (external link)
attribute time source_name String ISO_DateTime_UTC
attribute time standard_name String time
attribute time time_origin String 01-JAN-1970 00:00:00
attribute time time_precision String 1970-01-01T00:00:00Z
attribute time units String seconds since 1970-01-01T00:00:00Z

 
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