BCO-DMO ERDDAP
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Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Dinoflagellate culturing:\\u00a0Alexandrium fundyense (strain CCMP 1911) was\nobtained from the National Center for Marine Algae and Microbiota (NCMA). The\nstrain was originally isolated from Sequim Bay, Washington, USA. Heterocapsa\nrotundata (strain K-0483), obtained from the Scandanavian Culture Collection\nof Algae and Protozoa, was isolated from the southern Kattegat Sea near\nDenmark. Dinoflagellate cultures were maintained in f/2 medium without added\nsilicate at 15\\u00b0C under a 12L:12D light cycle and transferred every two to\nthree weeks into new media. Growth irradiance for A. fundyense was 53\n\\u00b5mol photons m-2 s-1; growth irradiance for H. rotundata was 12 \\u00b5mol\nphotons m-2 s-1.\n \nCell concentration and cell volume: Cell concentration (cells mL-1) and cell\nvolume (\\u00b5m3 cell-1) estimates for H. rotundata were obtained from live\nsamples measured with a Beckman Coulter Z2 Particle Count and Size Analyzer\nwith Z2 AccuComp software. For A. fundyense, samples were preserved in acid\nLugol\\u2019s solution (final concentration 2%). Cells were counted in a 1 ml\nSedgewick Rafter chamber using light microscopy; cell volume was estimated\nfrom length and width of cells (n = 42 or 83 cells per sample) measured with\nLeica Application Suite X image analysis software and assuming cell shape was\napproximated by an oblate ellipsoid.\n \nChlorophyll-a: Chl-a concentrations were measured by filtering samples through\n0.7 \\u00b5m effective pore size 25 mm glass fiber filters. Pigments were then\nextracted in 90% acetone for 24 h (dark, -20\\u00b0C) and fluorescence was\nmeasured on a Turner 10-AU fluorometer using the acidification method.\nConcentrations are reported per cell and per unit cell volume.\n \nDissolved and particulate dimethylsulfoniopropionate (DMSP):\\u00a0 DMSP\nsamples (4 ml) were gravity-filtered through precombusted 0.7 \\u00b5m\neffective pore size 25 mm glass fiber filters so as not to rupture the cells.\nFor measurement of particulate (intracellular) DMSP (DMSPp), filters were\nplaced into sealed 20-ml glass vials containing 3 ml of 5 N NaOH. For\nmeasurement of DMSP in the dissolved (extracellular) phase (DMSPd), the first\n4.5 mL of each sample\\u2019s filtrate were caught in a 5 ml polystyrene\nculture tube, which was capped and stored at -80\\u00b0C. Later, DMSPd samples\nwere thawed and sparged with N2 gas for 1 min to remove any dimethyl sulfide\n(DMS) already present. Each sparged sample (4 ml) was then dispensed into a\n20-ml vial containing 1 ml of 5 N NaOH, and sealed. Upon being sealed,\nprepared vials for either analysis were allowed to equilibrate for at least 24\nh, allowing for the 1:1 conversion of DMSP to gaseous DMS, detectable via gas\nchromatography. Standards for DMSPp were prepared from pre-diluted DMSP\nsolutions at the same time that samples were filtered and sealed into vials,\nwhile DMSPd standards were made at the same time that samples were sparged and\nsealed into vials. Samples and standards were analyzed using a Shimadzu Gas\nChromatograph 14-A equipped with a flame photometric detector and a Supelco\npacked Chromosil 330 column. The chromatograph was operated isothermally at\n90\\u00b0C with flow rates of hydrogen, air, and helium (carrier gas) at 50,\n60, and 150 kPa, respectively. DMSPp samples and standards were measured via\ndirect injection while DMSPd samples and standards were measured with a\nheadspace sweep (He flow rate 40 kPa). DMSPp concentrations are reported per\ncell and per unit cell volume. DMSPd concentrations are reported per cell and\nper unit seawater volume.
attribute NC_GLOBAL awards_0_award_nid String 614837
attribute NC_GLOBAL awards_0_award_number String OCE-1434842
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1434842 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Dinoflagellate culturing \n  S. Strom, PI \n  Version 11 January 2018
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3  19 Dec 2019
attribute NC_GLOBAL date_created String 2018-01-11T20:06:39Z
attribute NC_GLOBAL date_modified String 2019-03-15T20:12:34Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.723277.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/723277 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Gas Chromatograph
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to analyze samples and standards
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 723350
attribute NC_GLOBAL instruments_0_description String Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC)
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB02/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Gas Chromatograph
attribute NC_GLOBAL instruments_0_instrument_nid String 661
attribute NC_GLOBAL instruments_0_supplied_name String Shimadzu Gas Chromatograph
attribute NC_GLOBAL instruments_1_acronym String ACC
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Used to determine cell concentration
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 723351
attribute NC_GLOBAL instruments_1_description String Automated Cell Counter (ACC) - a tool used for counting live and/or dead cells in a culture.  It can also be used to size particles.
attribute NC_GLOBAL instruments_1_instrument_name String Automated Cell Counter
attribute NC_GLOBAL instruments_1_instrument_nid String 651528
attribute NC_GLOBAL instruments_1_supplied_name String Beckman Coulter Z2 Particle Count and Size Analyzer
attribute NC_GLOBAL keywords String available, bco, bco-dmo, biological, cell, cellvol1, chemical, chemistry, chlorophyll, concentration, concentration_of_chlorophyll_in_sea_water, counts, data, dataset, date, defense, determinations, dinoflagellate, Dinoflagellate_species, dissolved, dmo, dmsp, during, earth, Earth Science > Oceans > Ocean Chemistry > Chlorophyll, erddap, experiment, Experiment_ID, exposure, fmolcell1, management, meteorological, mmol, nmol, ocean, oceanography, oceans, office, par, particulate, photosynthetically, preliminary, program, radiation, replicate, Replicate_cell_counts, Replicate_cell_volume, Replicate_determinations_chlorophyll_a, Replicate_determinations_chlorophyll_a_per_unit_cell_volume, Replicate_determinations_dissolved_DMSP_fmolcell1, Replicate_determinations_dissolved_DMSP_nmolL1, Replicate_determinations_particulate_DMSP_fmolcell1, Replicate_determinations_particulate_DMSP_mmolL_cellvol1, Replicate_determinations_total_DMSP, sampling, Sampling_timepoint, satellite, science, sea, seawater, species, time, timepoint, total, Total_PAR_during_exposure_treatment, treatment, volume, water
attribute NC_GLOBAL keywords_vocabulary String GCMD Science Keywords
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/723277/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/723277 (external link)
attribute NC_GLOBAL param_mapping String {'723277': {'Experiment_Date': 'flag - time'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/723277/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Western Washington University - Shannon Point Marine Center
attribute NC_GLOBAL people_0_affiliation_acronym String SPMC
attribute NC_GLOBAL people_0_person_name String Suzanne Strom
attribute NC_GLOBAL people_0_person_nid String 50471
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_1_person_name String Hannah Ake
attribute NC_GLOBAL people_1_person_nid String 650173
attribute NC_GLOBAL people_1_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL project String Protist signaling
attribute NC_GLOBAL projects_0_acronym String Protist signaling
attribute NC_GLOBAL projects_0_description String Description from NSF proposal:\nThis proposal arises from the central premise that the oxidative stress response is an emergent property of phototrophic cellular systems, with implications for nearly every aspect of a phytoplankton cell’s life in the upper ocean. Oxidative stress (OS) arises from the uncompensated production of reactive oxygen species (ROS) within a cell, which can occur in response to a myriad of environmental stressors (e.g. nutrient limitation, temperature extremes, toxins, variable light exposure). In addition to the biochemical damage and physiological impairment that OS can cause, the phytoplankton OS response also includes increased net production and extracellular release of ROS, osmolytes, and other compounds that are known or suspected to be potent signals regulating protist behavior. We hypothesize that, through chemical signaling, oxidative stress acts to govern relationships among environmental variability, phytoplankton condition, and protist predation. Our proposed study of these integrated signaling and response processes has three overarching objectives: 1) Create and characterize oxidatively stressed phytoplankton. We will use light stress (variable exposure to visible light and UV) to create oxidatively stressed phytoplankton in the laboratory. Common coastal taxa with contrasting stress responses will be characterized using an array of fluorescent probes, biochemical measurements, and physiological assays. In addition, intracellular production and extracellular release of ROS and the associated chemical signal DMSP will be quantified. Use of Phaeodactylum tricornutum light stress mutants will add an independent means of connecting OS to signal production and predation response. 2) Examine protist predator responses to oxidatively stressed phytoplankton and associated chemical signals. Responses will be investigated by means of manipulation experiments and thorough characterization of associated signal chemistry. Assessment of predator response will be via predation rate measurements and population aggregation/dispersal behaviors in structured columns. 3) Investigate the prevalence of OS, its environmental correlates, and the microzooplankton predation response in the natural waters of a well-characterized local embayment. Application of ROS probes and OS assays to the natural environment and the design of OS manipulation experiments will be informed by the laboratory experiments using local protist species.\nOur work will help to elucidate some of the multiple ways in which the OS response can affect phytoplankton fitness, contributing information that can be used to characterize the position of key coastal species along an OS response spectrum. Ultimately such information could be used in trait-based conceptual and numerical models in a manner analogous to cell size and other 'master traits'. Our research will also inform the relatively new and exciting field of chemical signaling in planktonic communities, exploring DMSP- and ROS-based signaling between two of the most significant groups in the plankton, the eukaryotic phytoplankton and their protist predators. Finally, findings will help elucidate the links between environmental stress, phytoplankton response, and predation in planktonic ecosystems. These links relate to central issues in biological oceanography, including the predator-prey interactions that influence bloom demise, and the mechanisms by which protists feed selectively and thereby structure prey communities. The proposed research is a cross-cutting endeavor that unites subjects usually studied in isolation through a novel conceptual framework. Thus the findings have the potential to generate broadly applicable new insights into the ecological and evolutionary regulation of this key trophic link in planktonic food webs.
attribute NC_GLOBAL projects_0_end_date String 2017-08
attribute NC_GLOBAL projects_0_geolocation String Salish Sea: 48.5, -122.75
attribute NC_GLOBAL projects_0_name String Environmental stress and signaling based on reactive oxygen species among planktonic protists
attribute NC_GLOBAL projects_0_project_nid String 614838
attribute NC_GLOBAL projects_0_start_date String 2014-09
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Size and chemical responses of two dinoflagellate species used in natural high light exposure experiments (Protist Signaling project)
attribute NC_GLOBAL time_coverage_end String 2015-09-10
attribute NC_GLOBAL time_coverage_start String 2015-07-15
attribute NC_GLOBAL title String [Dinoflagellate culturing] - Size and chemical responses of two dinoflagellate species used in natural high light exposure experiments (Protist Signaling project) (Environmental stress and signaling based on reactive oxygen species among planktonic protists)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable Experiment_ID String
attribute Experiment_ID bcodmo_name String exp_id
attribute Experiment_ID description String Experiment ID number
attribute Experiment_ID long_name String Experiment ID
attribute Experiment_ID units String unitless
variable time double
attribute time _CoordinateAxisType String Time
attribute time actual_range double 1.4369184E9, 1.4418432E9
attribute time axis String T
attribute time bcodmo_name String date
attribute time description String Experiment date; YYYY/MM/DD
attribute time ioos_category String Time
attribute time long_name String Experiment Date
attribute time nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/ (external link)
attribute time source_name String Experiment_Date
attribute time standard_name String time
attribute time time_origin String 01-JAN-1970 00:00:00
attribute time time_precision String 1970-01-01
attribute time units String seconds since 1970-01-01T00:00:00Z
variable Dinoflagellate_species String
attribute Dinoflagellate_species bcodmo_name String species
attribute Dinoflagellate_species description String Species of sample
attribute Dinoflagellate_species long_name String Dinoflagellate Species
attribute Dinoflagellate_species units String unitless
variable Total_PAR_during_exposure_treatment float
attribute Total_PAR_during_exposure_treatment _FillValue float NaN
attribute Total_PAR_during_exposure_treatment actual_range float 0.004, 9.897
attribute Total_PAR_during_exposure_treatment bcodmo_name String PAR
attribute Total_PAR_during_exposure_treatment description String Total dose of photosynthetically active radiation received during light exposure treatment
attribute Total_PAR_during_exposure_treatment long_name String Total PAR During Exposure Treatment
attribute Total_PAR_during_exposure_treatment units String mol photons m^-2
variable Sampling_timepoint String
attribute Sampling_timepoint bcodmo_name String unknown
attribute Sampling_timepoint description String Time sample was taken
attribute Sampling_timepoint long_name String Sampling Timepoint
attribute Sampling_timepoint units String unitless
variable Replicate_cell_counts int
attribute Replicate_cell_counts _FillValue int 2147483647
attribute Replicate_cell_counts actual_range int 1603, 34083
attribute Replicate_cell_counts bcodmo_name String cell_concentration
attribute Replicate_cell_counts description String Cell concentration in seawater media
attribute Replicate_cell_counts long_name String Replicate Cell Counts
attribute Replicate_cell_counts units String cells ml-1
variable Replicate_cell_volume float
attribute Replicate_cell_volume _FillValue float NaN
attribute Replicate_cell_volume actual_range float 119.98, 23641.0
attribute Replicate_cell_volume bcodmo_name String volume
attribute Replicate_cell_volume description String Volume of individual dinoflagellate cells
attribute Replicate_cell_volume long_name String Replicate Cell Volume
attribute Replicate_cell_volume units String um^3
variable Replicate_determinations_chlorophyll_a float
attribute Replicate_determinations_chlorophyll_a _FillValue float NaN
attribute Replicate_determinations_chlorophyll_a actual_range float 0.22, 42.73
attribute Replicate_determinations_chlorophyll_a bcodmo_name String chlorophyll a
attribute Replicate_determinations_chlorophyll_a colorBarMaximum double 30.0
attribute Replicate_determinations_chlorophyll_a colorBarMinimum double 0.03
attribute Replicate_determinations_chlorophyll_a colorBarScale String Log
attribute Replicate_determinations_chlorophyll_a description String Chlorophyll-a content of cells
attribute Replicate_determinations_chlorophyll_a long_name String Concentration Of Chlorophyll In Sea Water
attribute Replicate_determinations_chlorophyll_a nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLHPP1/ (external link)
attribute Replicate_determinations_chlorophyll_a units String pg cell-1
variable Replicate_determinations_chlorophyll_a_per_unit_cell_volume float
attribute Replicate_determinations_chlorophyll_a_per_unit_cell_volume _FillValue float NaN
attribute Replicate_determinations_chlorophyll_a_per_unit_cell_volume actual_range float 1314.94, 8746.33
attribute Replicate_determinations_chlorophyll_a_per_unit_cell_volume bcodmo_name String chlorophyll a
attribute Replicate_determinations_chlorophyll_a_per_unit_cell_volume colorBarMaximum double 30.0
attribute Replicate_determinations_chlorophyll_a_per_unit_cell_volume colorBarMinimum double 0.03
attribute Replicate_determinations_chlorophyll_a_per_unit_cell_volume colorBarScale String Log
attribute Replicate_determinations_chlorophyll_a_per_unit_cell_volume description String Intracellular concentration of chlorophyll-a
attribute Replicate_determinations_chlorophyll_a_per_unit_cell_volume long_name String Concentration Of Chlorophyll In Sea Water
attribute Replicate_determinations_chlorophyll_a_per_unit_cell_volume nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLHPP1/ (external link)
attribute Replicate_determinations_chlorophyll_a_per_unit_cell_volume units String mg L-1
variable Replicate_determinations_particulate_DMSP_fmolcell1 float
attribute Replicate_determinations_particulate_DMSP_fmolcell1 _FillValue float NaN
attribute Replicate_determinations_particulate_DMSP_fmolcell1 actual_range float 10.52, 3986.6
attribute Replicate_determinations_particulate_DMSP_fmolcell1 bcodmo_name String dimethylsulphoniopropionate
attribute Replicate_determinations_particulate_DMSP_fmolcell1 description String Dimethylsulfoniopropionate content of cells
attribute Replicate_determinations_particulate_DMSP_fmolcell1 long_name String Replicate Determinations Particulate DMSP Fmolcell1
attribute Replicate_determinations_particulate_DMSP_fmolcell1 units String fmol cell-1
variable Replicate_determinations_particulate_DMSP_mmolL_cellvol1 float
attribute Replicate_determinations_particulate_DMSP_mmolL_cellvol1 _FillValue float NaN
attribute Replicate_determinations_particulate_DMSP_mmolL_cellvol1 actual_range float 71.34, 199.38
attribute Replicate_determinations_particulate_DMSP_mmolL_cellvol1 bcodmo_name String dimethylsulphoniopropionate
attribute Replicate_determinations_particulate_DMSP_mmolL_cellvol1 description String Intracellular concentration of dimethylsulfoniopropionate
attribute Replicate_determinations_particulate_DMSP_mmolL_cellvol1 long_name String Replicate Determinations Particulate DMSP Mmol L Cellvol1
attribute Replicate_determinations_particulate_DMSP_mmolL_cellvol1 units String mmol L cell vol-1
variable Replicate_determinations_dissolved_DMSP_nmolL1 float
attribute Replicate_determinations_dissolved_DMSP_nmolL1 _FillValue float NaN
attribute Replicate_determinations_dissolved_DMSP_nmolL1 actual_range float 7.11, 1037.85
attribute Replicate_determinations_dissolved_DMSP_nmolL1 bcodmo_name String dimethylsulphoniopropionate
attribute Replicate_determinations_dissolved_DMSP_nmolL1 description String Concentration of dimethylsulfoniopropionate dissolved in seawater medium normalized to cell concentration in medium
attribute Replicate_determinations_dissolved_DMSP_nmolL1 long_name String Replicate Determinations Dissolved DMSP Nmol L1
attribute Replicate_determinations_dissolved_DMSP_nmolL1 units String nmol L-1
variable Replicate_determinations_dissolved_DMSP_fmolcell1 float
attribute Replicate_determinations_dissolved_DMSP_fmolcell1 _FillValue float NaN
attribute Replicate_determinations_dissolved_DMSP_fmolcell1 actual_range float 0.27, 601.65
attribute Replicate_determinations_dissolved_DMSP_fmolcell1 bcodmo_name String dimethylsulphoniopropionate
attribute Replicate_determinations_dissolved_DMSP_fmolcell1 description String Concentration of dimethylsulfoniopropionate dissolved in seawater medium normalized to cell concentration in medium
attribute Replicate_determinations_dissolved_DMSP_fmolcell1 long_name String Replicate Determinations Dissolved DMSP Fmolcell1
attribute Replicate_determinations_dissolved_DMSP_fmolcell1 units String fmol cell-1
variable Replicate_determinations_total_DMSP float
attribute Replicate_determinations_total_DMSP _FillValue float NaN
attribute Replicate_determinations_total_DMSP actual_range float 0.01, 9.28
attribute Replicate_determinations_total_DMSP bcodmo_name String dimethylsulphoniopropionate
attribute Replicate_determinations_total_DMSP description String Concentration of dissolved plus particulate dimethylsulfoniopropionate
attribute Replicate_determinations_total_DMSP long_name String Replicate Determinations Total DMSP
attribute Replicate_determinations_total_DMSP units String umol L-1

 
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