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Row Type | Variable Name | Attribute Name | Data Type | Value |
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attribute | NC_GLOBAL | access_formats | String | .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson,.odvTxt |
attribute | NC_GLOBAL | acquisition_description | String | Water was collected via Niskin bottles mounted on a rosette, equipped with a\nCTD.\n \nExperiments on (operationally defined) particles were carried out by gravity-\nfiltering water through 3 \\u00b5m pore size filters.\\u00a0 1/12th sections of\nthe 3 \\u00b5m pore-size filters were submerged in 4 mL artificial seawater in\nincubation cuvettes.\\u00a0 Particle-associated peptidase and glucosidase\nactivity assays were set up in 4 mL cuvettes. For live duplicate incubations,\ntwo particle-containing filter pieces (each 1/12th of entire filter) were\nseparately submerged in 4 mL of cooled, autoclaved ambient seawater. A single\nkilled control was prepared by submerging a sterile filter piece (1/12th of\nunused filter) in 4 mL of cooled, autoclaved ambient seawater. Substrates were\nadded to a final concentration of 100 \\u00b5M. At various timepoints\\u2014upon\naddition of substrate (to), 24 h (t1), 48 (t2), and 72 h (t3)\\u2014live\nduplicates and killed control singleton were subsampled by taking 3 x 200 uL\n(for technical triplicates) per incubation, and placed in a 96 well plate for\nfluorescence measurement using the Tecan Plate Reader. Fluorescence values\nwere converted to hydrolysis rates using calibration curves with the MCA and\nMUF fluorophores, and were\\u00a0 normalized by the volume of filtrate that\npassed through the 3 \\u00b5m filter. Two substrates, -glucose and -glucose\nlinked to a 4-methylumbelliferyl (MUF) fluorophore, were used to measure\nglucosidase activities. Five substrates linked to a 7-amido-4-methyl coumarin\n(MCA) fluorophore, one amino acid \\u2013 leucine \\u2013 and four oligopeptides\n\\u2013 the chymotrypsin substrates alanine-alanine-phenylalanine (AAF) and\nalanine-alanine-proline-phenylalanine (AAPF), and the trypsin substrates\nglutamine-alanine-arginine (QAR) and phenylalanine-serine-arginine (FSR)\n\\u2013 were used to measure exo- and endo-acting peptidase activities,\nrespectively. Hydrolysis rates of the substrates were measured as an increase\nin fluorescence as the fluorophore was hydrolyzed from the substrate over time\n[as in Hoppe, 1993; Obayashi and Suzuki, 2005].\n \na-glu = substrate to measure alpha glucosidase:\n4-methylumbelliferyl-a-D-glucopyranoside \n b-glu = substrate to measure beta glucosidase:\n4-methylumbelliferyl-\\u00df-D-glucopyranoside \n L = substrate to measure leucine aminopeptidase (L-leucine-7-amido-4 MCA) \n AAF = substrate to measure chymotrypsin activity: ala-ala-phe-MCA \n AAPF = substrate to measure chymotrypsin activity: N-succinyl-ala-ala-pro-\nphe-MCA \n QAR = substrate to measure trypsin activity: Boc-gln-ala-arg-MCA\\u00a0\\u00a0 \n FSR = substrate to measure trypsin activity: N-t-boc-phe-ser-arg-MCA\\u00a0 |
attribute | NC_GLOBAL | awards_0_award_nid | String | 712358 |
attribute | NC_GLOBAL | awards_0_award_number | String | OCE-1332881 |
attribute | NC_GLOBAL | awards_0_data_url | String | http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1332881 |
attribute | NC_GLOBAL | awards_0_funder_name | String | NSF Division of Ocean Sciences |
attribute | NC_GLOBAL | awards_0_funding_acronym | String | NSF OCE |
attribute | NC_GLOBAL | awards_0_funding_source_nid | String | 355 |
attribute | NC_GLOBAL | awards_0_program_manager | String | Henrietta N Edmonds |
attribute | NC_GLOBAL | awards_0_program_manager_nid | String | 51517 |
attribute | NC_GLOBAL | cdm_data_type | String | Other |
attribute | NC_GLOBAL | comment | String | Microbial Activities: GF MCAMUF \n RV/Sonne So248, South and North Pacific, along 180 W \n P.I.: C. Arnosti (UNC-CH) \n version: 2018-07-31 \n NOTE: Date/time and location data from CTD cast data (https://doi.pangaea.de/10.1594/PANGAEA.864673) were joined to original dataset |
attribute | NC_GLOBAL | Conventions | String | COARDS, CF-1.6, ACDD-1.3 |
attribute | NC_GLOBAL | creator_email | String | info at bco-dmo.org |
attribute | NC_GLOBAL | creator_name | String | BCO-DMO |
attribute | NC_GLOBAL | creator_type | String | institution |
attribute | NC_GLOBAL | creator_url | String | https://www.bco-dmo.org/ |
attribute | NC_GLOBAL | data_source | String | extract_data_as_tsv version 2.3 19 Dec 2019 |
attribute | NC_GLOBAL | dataset_current_state | String | Final and no updates |
attribute | NC_GLOBAL | date_created | String | 2018-08-06T16:56:25Z |
attribute | NC_GLOBAL | date_modified | String | 2020-04-27T18:19:40Z |
attribute | NC_GLOBAL | defaultDataQuery | String | &time<now |
attribute | NC_GLOBAL | doi | String | 10.26008/1912/bco-dmo.743320.1 |
attribute | NC_GLOBAL | Easternmost_Easting | double | 180.0 |
attribute | NC_GLOBAL | geospatial_lat_max | double | 56.9999 |
attribute | NC_GLOBAL | geospatial_lat_min | double | -30.0008 |
attribute | NC_GLOBAL | geospatial_lat_units | String | degrees_north |
attribute | NC_GLOBAL | geospatial_lon_max | double | 180.0 |
attribute | NC_GLOBAL | geospatial_lon_min | double | -176.4753 |
attribute | NC_GLOBAL | geospatial_lon_units | String | degrees_east |
attribute | NC_GLOBAL | geospatial_vertical_max | double | 1000.0 |
attribute | NC_GLOBAL | geospatial_vertical_min | double | 60.0 |
attribute | NC_GLOBAL | geospatial_vertical_positive | String | down |
attribute | NC_GLOBAL | geospatial_vertical_units | String | m |
attribute | NC_GLOBAL | infoUrl | String | https://www.bco-dmo.org/dataset/743320 |
attribute | NC_GLOBAL | institution | String | BCO-DMO |
attribute | NC_GLOBAL | instruments_0_acronym | String | Niskin bottle |
attribute | NC_GLOBAL | instruments_0_dataset_instrument_nid | String | 743326 |
attribute | NC_GLOBAL | instruments_0_description | String | A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. |
attribute | NC_GLOBAL | instruments_0_instrument_external_identifier | String | https://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/ |
attribute | NC_GLOBAL | instruments_0_instrument_name | String | Niskin bottle |
attribute | NC_GLOBAL | instruments_0_instrument_nid | String | 413 |
attribute | NC_GLOBAL | instruments_1_acronym | String | CTD |
attribute | NC_GLOBAL | instruments_1_dataset_instrument_nid | String | 743327 |
attribute | NC_GLOBAL | instruments_1_description | String | The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column and permits scientists observe the physical properties in real time via a conducting cable connecting the CTD to a deck unit and computer on the ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast. This instrument designation is used when specific make and model are not known. |
attribute | NC_GLOBAL | instruments_1_instrument_external_identifier | String | https://vocab.nerc.ac.uk/collection/L05/current/130/ |
attribute | NC_GLOBAL | instruments_1_instrument_name | String | CTD profiler |
attribute | NC_GLOBAL | instruments_1_instrument_nid | String | 417 |
attribute | NC_GLOBAL | instruments_2_acronym | String | Fluorometer |
attribute | NC_GLOBAL | instruments_2_dataset_instrument_nid | String | 743328 |
attribute | NC_GLOBAL | instruments_2_description | String | A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. |
attribute | NC_GLOBAL | instruments_2_instrument_external_identifier | String | https://vocab.nerc.ac.uk/collection/L05/current/113/ |
attribute | NC_GLOBAL | instruments_2_instrument_name | String | Fluorometer |
attribute | NC_GLOBAL | instruments_2_instrument_nid | String | 484 |
attribute | NC_GLOBAL | keywords | String | average, bco, bco-dmo, biological, cast, cast_no, chemical, data, dataset, date, depth, depth_m, depth_no, dev, dmo, elapsed, erddap, filter, filter_um, iso, latitude, longitude, management, oceanography, office, preliminary, profiler, rate, rep1, rep1_rate, rep2, rep2_rate, salinity, salinity-temperature-depth, station, station_no, std, std_dev, substrate, temperature, time, time_elapsed_hr, timepoint |
attribute | NC_GLOBAL | license | String | https://www.bco-dmo.org/dataset/743320/license |
attribute | NC_GLOBAL | metadata_source | String | https://www.bco-dmo.org/api/dataset/743320 |
attribute | NC_GLOBAL | Northernmost_Northing | double | 56.9999 |
attribute | NC_GLOBAL | param_mapping | String | {'743320': {'Latitude': 'flag - latitude', 'depth_m': 'flag - depth', 'Longitude': 'flag - longitude', 'ISO_DateTime_UTC': 'flag - time'}} |
attribute | NC_GLOBAL | parameter_source | String | https://www.bco-dmo.org/mapserver/dataset/743320/parameters |
attribute | NC_GLOBAL | people_0_affiliation | String | University of North Carolina at Chapel Hill |
attribute | NC_GLOBAL | people_0_affiliation_acronym | String | UNC-Chapel Hill |
attribute | NC_GLOBAL | people_0_person_name | String | Carol Arnosti |
attribute | NC_GLOBAL | people_0_person_nid | String | 661940 |
attribute | NC_GLOBAL | people_0_role | String | Principal Investigator |
attribute | NC_GLOBAL | people_0_role_type | String | originator |
attribute | NC_GLOBAL | people_1_affiliation | String | Woods Hole Oceanographic Institution |
attribute | NC_GLOBAL | people_1_affiliation_acronym | String | WHOI BCO-DMO |
attribute | NC_GLOBAL | people_1_person_name | String | Nancy Copley |
attribute | NC_GLOBAL | people_1_person_nid | String | 50396 |
attribute | NC_GLOBAL | people_1_role | String | BCO-DMO Data Manager |
attribute | NC_GLOBAL | people_1_role_type | String | related |
attribute | NC_GLOBAL | project | String | Patterns of activities |
attribute | NC_GLOBAL | projects_0_acronym | String | Patterns of activities |
attribute | NC_GLOBAL | projects_0_description | String | NSF Award Abstract:\nHeterotrophic microbial communities are key players in the marine carbon cycle, transforming and respiring organic carbon, regenerating nutrients, and acting as the final filter in sediments through which organic matter passes before long-term burial. Microbially-driven carbon cycling in the ocean profoundly affects the global carbon cycle, but key factors determining rates and locations of organic matter remineralization are unclear. In this study, researchers from the University of North Carolina at Chapel Hill will investigate the ability of pelagic microbial communities to initiate the remineralization of polysaccharides and proteins, which together constitute a major pool of organic matter in the ocean. Results from this study will be predictive on a large scale regarding the nature of the microbial response to organic matter input, and will provide a mechanistic framework for interpreting organic matter reactivity in the ocean.\nBroader Impacts: This study will provide scientific training for undergraduate and graduate students from underrepresented groups. The project will also involve German colleagues, thus strengthening international scientific collaboration. |
attribute | NC_GLOBAL | projects_0_end_date | String | 2017-07 |
attribute | NC_GLOBAL | projects_0_geolocation | String | Atlantic Ocean, Arctic Ocean, Pacific Ocean, Greenland |
attribute | NC_GLOBAL | projects_0_name | String | Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean? |
attribute | NC_GLOBAL | projects_0_project_nid | String | 712359 |
attribute | NC_GLOBAL | projects_0_start_date | String | 2013-08 |
attribute | NC_GLOBAL | publisher_name | String | Biological and Chemical Oceanographic Data Management Office (BCO-DMO) |
attribute | NC_GLOBAL | publisher_type | String | institution |
attribute | NC_GLOBAL | sourceUrl | String | (local files) |
attribute | NC_GLOBAL | Southernmost_Northing | double | -30.0008 |
attribute | NC_GLOBAL | standard_name_vocabulary | String | CF Standard Name Table v55 |
attribute | NC_GLOBAL | subsetVariables | String | rep1_rate,rep2_rate,filter_um |
attribute | NC_GLOBAL | summary | String | This dataset includes MCAMUF (glucosidase and peptidase) hydrolysis rates to measure microbial enzyme activities on particles collected from gravity filtered seawater. Samples were collected on RV/Sonne cruise SO248 in May 2016. Links to archived CTD data are also provided. |
attribute | NC_GLOBAL | time_coverage_end | String | 2016-05-29T21:43Z |
attribute | NC_GLOBAL | time_coverage_start | String | 2016-05-02T16:51Z |
attribute | NC_GLOBAL | title | String | [SO248: GF MCAMUF hydrolysis rates] - Microbial enzyme activities: glucosidase and peptidase activities of gravity filtered seawater samples from the RV\\Sonne cruise SO248 in the South and North Pacific, along 180 W, May, 2016 (Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean?) |
attribute | NC_GLOBAL | version | String | 1 |
attribute | NC_GLOBAL | Westernmost_Easting | double | -176.4753 |
attribute | NC_GLOBAL | xml_source | String | osprey2erddap.update_xml() v1.5 |
variable | station_no | String | ||
attribute | station_no | bcodmo_name | String | station |
attribute | station_no | description | String | refers to station number for cruise |
attribute | station_no | long_name | String | Station No |
attribute | station_no | units | String | unitless |
variable | cast_no | String | ||
attribute | cast_no | bcodmo_name | String | cast |
attribute | cast_no | description | String | cast number (refers to cast of CTD/Niskin bottles on cruise) |
attribute | cast_no | long_name | String | Cast No |
attribute | cast_no | units | String | unitless |
variable | time | double | ||
attribute | time | _CoordinateAxisType | String | Time |
attribute | time | actual_range | double | 1.46220786E9, 1.46455818E9 |
attribute | time | axis | String | T |
attribute | time | bcodmo_name | String | ISO_DateTime_UTC |
attribute | time | description | String | date and time in ISO format (yyyy-mm-ddTHH:MM:SS |
attribute | time | ioos_category | String | Time |
attribute | time | long_name | String | ISO Date Time UTC |
attribute | time | nerc_identifier | String | https://vocab.nerc.ac.uk/collection/P01/current/DTUT8601/ |
attribute | time | source_name | String | ISO_DateTime_UTC |
attribute | time | standard_name | String | time |
attribute | time | time_origin | String | 01-JAN-1970 00:00:00 |
attribute | time | time_precision | String | 1970-01-01T00:00Z |
attribute | time | units | String | seconds since 1970-01-01T00:00:00Z |
variable | latitude | double | ||
attribute | latitude | _CoordinateAxisType | String | Lat |
attribute | latitude | _FillValue | double | NaN |
attribute | latitude | actual_range | double | -30.0008, 56.9999 |
attribute | latitude | axis | String | Y |
attribute | latitude | bcodmo_name | String | latitude |
attribute | latitude | colorBarMaximum | double | 90.0 |
attribute | latitude | colorBarMinimum | double | -90.0 |
attribute | latitude | description | String | latitude; north is positive |
attribute | latitude | ioos_category | String | Location |
attribute | latitude | long_name | String | Latitude |
attribute | latitude | nerc_identifier | String | https://vocab.nerc.ac.uk/collection/P09/current/LATX/ |
attribute | latitude | standard_name | String | latitude |
attribute | latitude | units | String | degrees_north |
variable | longitude | double | ||
attribute | longitude | _CoordinateAxisType | String | Lon |
attribute | longitude | _FillValue | double | NaN |
attribute | longitude | actual_range | double | -176.4753, 180.0 |
attribute | longitude | axis | String | X |
attribute | longitude | bcodmo_name | String | longitude |
attribute | longitude | colorBarMaximum | double | 180.0 |
attribute | longitude | colorBarMinimum | double | -180.0 |
attribute | longitude | description | String | longitude; east is postivie |
attribute | longitude | ioos_category | String | Location |
attribute | longitude | long_name | String | Longitude |
attribute | longitude | nerc_identifier | String | https://vocab.nerc.ac.uk/collection/P09/current/LONX/ |
attribute | longitude | standard_name | String | longitude |
attribute | longitude | units | String | degrees_east |
variable | depth_no | String | ||
attribute | depth_no | bcodmo_name | String | unknown |
attribute | depth_no | description | String | sequence of depths sampled (1 is surface; higher numbers at greater depths) |
attribute | depth_no | long_name | String | Depth |
attribute | depth_no | standard_name | String | depth |
attribute | depth_no | units | String | unitless |
variable | depth | double | ||
attribute | depth | _CoordinateAxisType | String | Height |
attribute | depth | _CoordinateZisPositive | String | down |
attribute | depth | _FillValue | double | NaN |
attribute | depth | actual_range | double | 60.0, 1000.0 |
attribute | depth | axis | String | Z |
attribute | depth | bcodmo_name | String | depth |
attribute | depth | colorBarMaximum | double | 8000.0 |
attribute | depth | colorBarMinimum | double | -8000.0 |
attribute | depth | colorBarPalette | String | TopographyDepth |
attribute | depth | description | String | actual depth at which water collected |
attribute | depth | ioos_category | String | Location |
attribute | depth | long_name | String | Depth |
attribute | depth | nerc_identifier | String | https://vocab.nerc.ac.uk/collection/P09/current/DEPH/ |
attribute | depth | positive | String | down |
attribute | depth | standard_name | String | depth |
attribute | depth | units | String | m |
variable | substrate | String | ||
attribute | substrate | bcodmo_name | String | unknown |
attribute | substrate | description | String | Substrates for measurement of enzymatic activities:\na-glu = substrate to measure alpha glucosidase: 4-methylumbelliferyl-a-D-glucopyranoside\nb-glu = substrate to measure beta glucosidase: 4-methylumbelliferyl-ß-D-glucopyranoside\nL = substrate to measure leucine aminopeptidase (L-leucine-7-amido-4 MCA)\nAAF = substrate to measure chymotrypsin activity: ala-ala-phe-MCA\nAAPF = substrate to measure chymotrypsin activity: N-succinyl-ala-ala-pro-phe-MCA\nQAR = substrate to measure trypsin activity: Boc-gln-ala-arg-MCA \nFSR = substrate to measure trypsin activity: N-t-boc-phe-ser-arg-MCA |
attribute | substrate | long_name | String | Substrate |
attribute | substrate | units | String | unitless |
variable | timepoint | byte | ||
attribute | timepoint | _FillValue | byte | 127 |
attribute | timepoint | actual_range | byte | 1, 3 |
attribute | timepoint | bcodmo_name | String | time_point |
attribute | timepoint | description | String | sampling point post-incubation |
attribute | timepoint | long_name | String | Timepoint |
attribute | timepoint | units | String | unitless |
variable | time_elapsed_hr | float | ||
attribute | time_elapsed_hr | _FillValue | float | NaN |
attribute | time_elapsed_hr | actual_range | float | 12.0, 72.8 |
attribute | time_elapsed_hr | bcodmo_name | String | time_elapsed |
attribute | time_elapsed_hr | description | String | incubation time |
attribute | time_elapsed_hr | long_name | String | Time Elapsed Hr |
attribute | time_elapsed_hr | nerc_identifier | String | https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/ |
attribute | time_elapsed_hr | units | String | hours |
variable | rep1_rate | double | ||
attribute | rep1_rate | _FillValue | double | NaN |
attribute | rep1_rate | bcodmo_name | String | unknown |
attribute | rep1_rate | description | String | replicate 1 hydrolysis rate |
attribute | rep1_rate | long_name | String | Rep1 Rate |
attribute | rep1_rate | units | String | nanomoles/liter/hour (nmol L-1 h-1) |
variable | rep2_rate | double | ||
attribute | rep2_rate | _FillValue | double | NaN |
attribute | rep2_rate | bcodmo_name | String | unknown |
attribute | rep2_rate | description | String | replicate 2 hydrolysis rate |
attribute | rep2_rate | long_name | String | Rep2 Rate |
attribute | rep2_rate | units | String | nanomoles/liter/hour (nmol L-1 h-1) |
variable | average | float | ||
attribute | average | _FillValue | float | NaN |
attribute | average | actual_range | float | 0.0, 3.348693 |
attribute | average | bcodmo_name | String | unknown |
attribute | average | description | String | average of hydrolysis rates |
attribute | average | long_name | String | Average |
attribute | average | units | String | nanomoles/liter/hour (nmol L-1 h-1) |
variable | std_dev | float | ||
attribute | std_dev | _FillValue | float | NaN |
attribute | std_dev | actual_range | float | 0.0, 0.820532 |
attribute | std_dev | bcodmo_name | String | unknown |
attribute | std_dev | description | String | std deviation of hydrolysis rates |
attribute | std_dev | long_name | String | Std Dev |
attribute | std_dev | units | String | nanomoles/liter/hour (nmol L-1 h-1) |
variable | filter_um | byte | ||
attribute | filter_um | _FillValue | byte | 127 |
attribute | filter_um | actual_range | byte | 3, 3 |
attribute | filter_um | bcodmo_name | String | filter_size |
attribute | filter_um | description | String | filter pore size used for gravity filtration |
attribute | filter_um | long_name | String | Filter Um |
attribute | filter_um | units | String | micrometers |