BCO-DMO ERDDAP
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Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Hydrolytic enzyme activities were determined using\nL-leucine-4-methylcoumarinyl-7-amide (MCA) hydrochloride, 4-methylumbelliferyl\n\\u03b1-D-glucopyranoside, and 4-methylumbelliferone (MUF)\n\\u03b2-D-glucopyranoside (Sigma-Aldrich) as substrate proxies for leucine-\naminopeptidase, \\u03b1-glucosidase, and \\u03b2-glucosidase activities,\nrespectively. For each bottle and substrate proxy, 196 \\u00b5L of unfiltered\nexperimental or control water was added in duplicate to a pure-grade black\n96-well plate (Brand Life Sciences) containing a single substrate proxy at\nsaturation levels (final concentration 200 \\u00b5M). Fluorescence (excitation\n370 nm, emission 440 nm) was measured in a Tecan Infinite 200 Pro microplate\nreader immediately following the addition of the substrate and several more\ntimes over 7-20 h. The well plates were incubated in the dark at in situ\ntemperature. MUF and MCA standard solutions prepared in seawater were used to\ndetermine hydrolysis rates. Killed controls (boiled sample water) and\nultrapure water samples showed little change over the incubations.
attribute NC_GLOBAL awards_0_award_nid String 734588
attribute NC_GLOBAL awards_0_award_number String OCE-1459406
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1459406 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Henrietta N Edmonds
attribute NC_GLOBAL awards_0_program_manager_nid String 51517
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Coscinodiscus hydrolytic enzyme activities during CDOM monoculture experiment \n   PI: K. Ziervogel (UNH) \n   version: 2018-10-17
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3  19 Dec 2019
attribute NC_GLOBAL date_created String 2018-10-18T13:20:58Z
attribute NC_GLOBAL date_modified String 2019-03-18T15:43:27Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.748445.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/748445 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Flow Cytometer
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to make cell counts.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 748450
attribute NC_GLOBAL instruments_0_description String Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.\n(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB37/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Flow Cytometer
attribute NC_GLOBAL instruments_0_instrument_nid String 660
attribute NC_GLOBAL instruments_0_supplied_name String FACSCalibur flow cytometer (Becton-Dickson)
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Used to measure fluorescence from which hydrolysis rates were calculated.
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 748451
attribute NC_GLOBAL instruments_1_description String Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: https://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.
attribute NC_GLOBAL instruments_1_instrument_name String plate reader
attribute NC_GLOBAL instruments_1_instrument_nid String 528693
attribute NC_GLOBAL instruments_1_supplied_name String Tecan Infinite 200 Pro microplate reader
attribute NC_GLOBAL keywords String activity, bco, bco-dmo, biological, chemical, data, dataset, dmo, enz, enz_activity_t0, enz_activity_t1, enz_activity_t2, enz_activity_t3, erddap, fluor, fluor_t0, fluor_t1, fluor_t2, fluor_t3, management, oceanography, office, preliminary, rsqr, sample, slope, substrate, time, time_t0, time_t1, time_t2, time_t3
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/748445/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/748445 (external link)
attribute NC_GLOBAL param_mapping String {'748445': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/748445/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of New Hampshire
attribute NC_GLOBAL people_0_affiliation_acronym String UNH
attribute NC_GLOBAL people_0_person_name String Kai Ziervogel
attribute NC_GLOBAL people_0_person_nid String 734583
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_1_person_name String Nancy Copley
attribute NC_GLOBAL people_1_person_nid String 50396
attribute NC_GLOBAL people_1_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL project String PlankDOM
attribute NC_GLOBAL projects_0_acronym String PlankDOM
attribute NC_GLOBAL projects_0_description String NSF abstract:\nChromophoric dissolved organic matter (CDOM) is a small but important fraction of the marine carbon pool that interacts with solar radiation and thus affects many photochemical and biological processes in the ocean. Despite its importance, the chemical basis for the formation of oceanic CDOM remains unclear. CDOM may be formed from two possible sources: 1) heterotrophic bacterial transformations of primary productivity (plankton-derived), or 2) terrestrially-derived. This project will examine the role of phytoplankton as a source of CDOM in the ocean by utilizing a powerful, new technique to measure particulate organic matter absorbance and fluorescence, discrete chemical measurements of probable precursors to planktonic CDOM, and enzymatic assays. Results of this research will provide new insights into the origin and production of planktonic CDOM and its transformation by heterotrophic bacteria. This research on CDOM will be shared broadly through a module at a North Carolina Aquarium, and streaming live feeds of shipboard activities to elementary school classrooms.\nTerrestrial and oceanic dissolved organic matter (DOM) differ in their chemical composition. Laboratory and open-ocean observations suggest that bacterial transformation of phytoplankton DOM produces humic-like CDOM signals that are visually similar to those in terrestrial CDOM. However, prior studies of oceanic CDOM using absorbance and fluorescence fit an electronic interaction (EI) model of intramolecular charge transfer (CT) reactions between donor and acceptor molecules common to partially-oxidized terrestrial molecules found in humic substances. This project will test the hypothesis that phytoplankton and bacteria provide a source of donors and acceptors that are microbially-transformed and linked, enabling CT contacts between them and creating oceanic CDOM. To address this, researchers will systematically study phytoplankton growth, including marine snow formation. A new technique for measuring base-extracted POM (BEPOM) absorbance and fluorescence will be used to incorporate planktonic CDOM results into the EI model, and supplemented with measurements of its probable chemical precursors. These experiments will improve understanding of how the production of CDOM in the ocean is linked to the optics and chemistry of planktonic CDOM formation. Determining the time course and extent of phytoplankton POM and DOM transformation by heterotrophic bacteria during the same phytoplankton growth experiments will provide an in-depth understanding as to how bacterial transformation of marine snow-associated planktonic organic matter drives CDOM production throughout the ocean.
attribute NC_GLOBAL projects_0_end_date String 2019-04
attribute NC_GLOBAL projects_0_geolocation String Northern Atlantic Ocean, 34.65 N, 69.63 W
attribute NC_GLOBAL projects_0_name String Collaborative Research: Planktonic Sources of Chromophoric Dissolved Organic Matter in Seawater
attribute NC_GLOBAL projects_0_project_nid String 734581
attribute NC_GLOBAL projects_0_start_date String 2015-05
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String This dataset is from a laboratory experiment. Four phytoplankton cultures and their associated bacterial communities were incubated in replicate roller bottles (1.9 L) over 3-6 weeks under laboratory conditions. Bacterial dynamics in the culture bottles were measured and correlated with geochemical parameters to determine the role of bacterial activities on the formation of CDOM in the cultures (Kinsey et al., 2018, see below).\\r\\n\\r\\nThe data include fluorescence and bacterial enzyme activity during CDOM Coscinodiscus monoculture experiments. Growth stages were initial and exponential.
attribute NC_GLOBAL title String [Hydrolytic enzyme activity - Coscinodiscus] - Hydrolytic enzyme activities during CDOM monoculture experiment with Coscinodiscus (Collaborative Research: Planktonic Sources of Chromophoric Dissolved Organic Matter in Seawater)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable substrate String
attribute substrate bcodmo_name String unknown
attribute substrate description String substrate for measuring enzyme activity: a-glu = 4-methylumbelliferyl a-D-glucopyranoside; b-glu = 4-methylumbelliferone (MUF) ß-D-glucopyranoside; leu = L-leucine-4-methylcoumarinyl-7-amide
attribute substrate long_name String Substrate
attribute substrate units String unitless
variable sample String
attribute sample bcodmo_name String sample
attribute sample description String sample identifier denoted as growth stage (days from start) replicate id
attribute sample long_name String Sample
attribute sample nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute sample units String unitless
variable fluor_t0 float
attribute fluor_t0 _FillValue float NaN
attribute fluor_t0 actual_range float -0.4, 654.83
attribute fluor_t0 bcodmo_name String fluorescence
attribute fluor_t0 description String fluorescence intensity at time 0
attribute fluor_t0 long_name String Fluor T0
attribute fluor_t0 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/ (external link)
attribute fluor_t0 units String relative fluorescence units
variable fluor_t1 float
attribute fluor_t1 _FillValue float NaN
attribute fluor_t1 actual_range float -0.83, 1099.15
attribute fluor_t1 bcodmo_name String fluorescence
attribute fluor_t1 description String fluorescence intensity at time 1
attribute fluor_t1 long_name String Fluor T1
attribute fluor_t1 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/ (external link)
attribute fluor_t1 units String relative fluorescence units
variable fluor_t2 float
attribute fluor_t2 _FillValue float NaN
attribute fluor_t2 actual_range float -0.76, 1746.21
attribute fluor_t2 bcodmo_name String fluorescence
attribute fluor_t2 description String fluorescence intensity at time 2
attribute fluor_t2 long_name String Fluor T2
attribute fluor_t2 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/ (external link)
attribute fluor_t2 units String relative fluorescence units
variable fluor_t3 float
attribute fluor_t3 _FillValue float NaN
attribute fluor_t3 actual_range float -0.16, 2074.2
attribute fluor_t3 bcodmo_name String fluorescence
attribute fluor_t3 description String fluorescence intensity at time 3
attribute fluor_t3 long_name String Fluor T3
attribute fluor_t3 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/ (external link)
attribute fluor_t3 units String relative fluorescence units
variable time_t0 float
attribute time_t0 _FillValue float NaN
attribute time_t0 actual_range float -0.01, 0.05
attribute time_t0 bcodmo_name String time_elapsed
attribute time_t0 description String time since start of experiment; time point 0
attribute time_t0 long_name String Time T0
attribute time_t0 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/ (external link)
attribute time_t0 units String hours
variable time_t1 float
attribute time_t1 _FillValue float NaN
attribute time_t1 actual_range float 0.04, 3.38
attribute time_t1 bcodmo_name String time_elapsed
attribute time_t1 description String time elapsed from start of experiment; time point 1
attribute time_t1 long_name String Time T1
attribute time_t1 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/ (external link)
attribute time_t1 units String hours
variable time_t2 float
attribute time_t2 _FillValue float NaN
attribute time_t2 actual_range float 0.04, 5.83
attribute time_t2 bcodmo_name String time_elapsed
attribute time_t2 description String time elapsed from start of experiment; time point 2
attribute time_t2 long_name String Time T2
attribute time_t2 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/ (external link)
attribute time_t2 units String hours
variable time_t3 float
attribute time_t3 _FillValue float NaN
attribute time_t3 actual_range float 0.04, 7.93
attribute time_t3 bcodmo_name String time_elapsed
attribute time_t3 description String time elapsed from start of experiment; time point 3
attribute time_t3 long_name String Time T3
attribute time_t3 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/ (external link)
attribute time_t3 units String hours
variable enz_activity_t0 float
attribute enz_activity_t0 _FillValue float NaN
attribute enz_activity_t0 actual_range float 0.0, 801.19
attribute enz_activity_t0 bcodmo_name String unknown
attribute enz_activity_t0 description String enzymatic activity at time 0
attribute enz_activity_t0 long_name String Enz Activity T0
attribute enz_activity_t0 units String nanoMol/hour
variable enz_activity_t1 float
attribute enz_activity_t1 _FillValue float NaN
attribute enz_activity_t1 actual_range float -0.01, 1340.68
attribute enz_activity_t1 bcodmo_name String unknown
attribute enz_activity_t1 description String enzymatic activity at time 1
attribute enz_activity_t1 long_name String Enz Activity T1
attribute enz_activity_t1 units String nanoMol/hour
variable enz_activity_t2 float
attribute enz_activity_t2 _FillValue float NaN
attribute enz_activity_t2 actual_range float -0.01, 2126.34
attribute enz_activity_t2 bcodmo_name String unknown
attribute enz_activity_t2 description String enzymatic activity at time 2
attribute enz_activity_t2 long_name String Enz Activity T2
attribute enz_activity_t2 units String nanoMol/hour
variable enz_activity_t3 float
attribute enz_activity_t3 _FillValue float NaN
attribute enz_activity_t3 actual_range float 0.0, 2524.58
attribute enz_activity_t3 bcodmo_name String unknown
attribute enz_activity_t3 description String enzymatic activity at time 3
attribute enz_activity_t3 long_name String Enz Activity T3
attribute enz_activity_t3 units String nanoMol/hour
variable SLOPE float
attribute SLOPE _FillValue float NaN
attribute SLOPE actual_range float -0.001, 269.15
attribute SLOPE bcodmo_name String unknown
attribute SLOPE description String the slope of the graph of fluorescence intensity vs substrate concentration
attribute SLOPE long_name String SLOPE
attribute SLOPE units String unitless
variable RSQR float
attribute RSQR _FillValue float NaN
attribute RSQR actual_range float 0.0, 1.0
attribute RSQR bcodmo_name String unknown
attribute RSQR description String the square of the correlation coefficient of fluorescence intensity vs substrate concentration
attribute RSQR long_name String RSQR
attribute RSQR units String unitless

 
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