BCO-DMO ERDDAP
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Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Spawning and fertilization\n \nWe collected adult sand dollars (D. excentricus) from Semiahmoo Bay, WA, on\nJuly 7, 2017, and maintained them in 14\\u00b0C continuous flowing seawater at\nthe Shannon Point Marine Center. On July 12, 2017, we induced twelve\nindividuals to spawn by injecting 1-mL of 0.5-M KCl into the coelom following\nmethods outlined by Strathmann (1987).\\u00a0 We then collected and mixed\nconcentrated gametes of four males and four females for fertilization. We\nadded five drops of sperm to 500-mL of filtered seawater and 5-mL of eggs. We\nplaced the fertilized eggs in 12\\u00b0C incubator and bubbled them with\nambient pCO2 condition for 12-hrs before dividing the embryos into pCO2\ntreatment conditions before gastrulation. We then counted and transferred the\nlarvae into jars with 1.5 L of nanopore filtered seawater at densities of 1-2\nindividuals mL-1.\n \nGrazing experiment\n \nTo assess the interactive effects of high temperature and pCO2 on Dunaliella\nexcentricus feeding behavior, our experimental design had six treatments with\nfour experimental jars (replicates) in each. The treatments combined three\nlevels of CO2: 400 ppmv (ambient atmospheric level), 800 ppmv (moderate\natmoshpheric level) and 1,500 ppmv (high atmospheric level), and two\ntemperatures: 12\\u00b0C (ambient temperature) and 17\\u00b0C (high\ntemperature). We fed D. tertiolecta at approximately 6,000 cells ml-1 to six-\narm stage larvae to evaluate feeding rates at each treatment condition.\n \nFor each replicate, a corresponding 150-mL control bottle containing only D.\ntertiolecta was also prepared. Feeding rate was estimated as ingestion rate by\nmeasuring the algal concentration (cells ml-1) at the beginning (T0) and after\n24 hours (Tf) in control bottles and experimental jars using a Sedgewick\nRafter Chamber (Stumpp et al., 2011). Ingestion rate (cells ind-1 hr-1) was\ncalculated as I = (Clearance rate) x (time-average algae concentration).\\u00a0\n \nLipid storage analysis\n \nAt the end of the long-term experiment, larval lipid index was assessed using\na procedure adapted from Talmage et. al (2010).\\u00a0 First, we randomly\nselected D. excentricus larvae from each treatment and stained them with Nile\nRed dissolved in acetone. Nile Red stains intracellular lipid droplets bright\nyellow. Larvae were exposed to the stain for \\u223c1.5 h, after which they\nwere photographed under an epi-fluorescent microscope (Leica 80i) within 4\nhours of being stained\\u00a0 (Ko et al., 2014). The lipid areas of\napproximately 5\\u221215 larvae per sample were measured using the ImageJ\nsoftware. The lipid index was calculated by dividing the area of the larva\nstomach containing the fluorescing lipids by the total stomach area (Talmage &\nGobler, 2010).\\u00a0
attribute NC_GLOBAL awards_0_award_nid String 684166
attribute NC_GLOBAL awards_0_award_number String OCE-1538626
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1538626 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_0_program_manager_nid String 50446
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Dendraster Lipid Analysis - OA Expt 2017 \n   S. Arellano, B. Olson, S. Yang (WWU) \n   version: 2019-01-14
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3  19 Dec 2019
attribute NC_GLOBAL date_created String 2019-01-16T21:40:53Z
attribute NC_GLOBAL date_modified String 2019-09-25T20:02:15Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.753036.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/753036 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to photograph larval intracellular lipid droplets.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 753043
attribute NC_GLOBAL instruments_0_description String Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB06/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Microscope-Fluorescence
attribute NC_GLOBAL instruments_0_instrument_nid String 695
attribute NC_GLOBAL instruments_0_supplied_name String epi-fluorescent microscope (Leica 80i)
attribute NC_GLOBAL keywords String area, bco, bco-dmo, biological, chemical, data, dataset, dmo, erddap, index, jar, jar_replicate, lipid, lipid_area, lipid_index, lipid_presence, management, oceanography, office, pH_treatment, preliminary, presence, replicate, stomach, stomach_area, temp_treatment, temperature, treatment
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/753036/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/753036 (external link)
attribute NC_GLOBAL param_mapping String {'753036': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/753036/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Western Washington University
attribute NC_GLOBAL people_0_affiliation_acronym String WWU
attribute NC_GLOBAL people_0_person_name String Shawn M Arellano
attribute NC_GLOBAL people_0_person_nid String 684169
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Western Washington University
attribute NC_GLOBAL people_1_affiliation_acronym String WWU
attribute NC_GLOBAL people_1_person_name String Dr Brady  M. Olson
attribute NC_GLOBAL people_1_person_nid String 51528
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Western Washington University
attribute NC_GLOBAL people_2_affiliation_acronym String WWU
attribute NC_GLOBAL people_2_person_name String Dr Sylvia Yang
attribute NC_GLOBAL people_2_person_nid String 684172
attribute NC_GLOBAL people_2_role String Co-Principal Investigator
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_3_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_3_person_name String Nancy Copley
attribute NC_GLOBAL people_3_person_nid String 50396
attribute NC_GLOBAL people_3_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL project String Climate stressors on larvae
attribute NC_GLOBAL projects_0_acronym String Climate stressors on larvae
attribute NC_GLOBAL projects_0_description String In the face of climate change, future distribution of animals will depend not only on whether they adjust to new conditions in their current habitat, but also on whether a species can spread to suitable locations in a changing habitat landscape. In the ocean, where most species have tiny drifting larval stages, dispersal between habitats is impacted by more than just ocean currents alone; the swimming behavior of larvae, the flow environment the larvae encounter, and the length of time the larvae spend in the water column all interact to impact the distance and direction of larval dispersal. The effects of climate change, especially ocean acidification, are already evident in shellfish species along the Pacific coast, where hatchery managers have noticed shellfish cultures with 'lazy larvae syndrome.' Under conditions of increased acidification, these 'lazy larvae' simply stop swimming; yet, larval swimming behavior is rarely incorporated into studies of ocean acidification. Furthermore, how ocean warming interacts with the effects of acidification on larvae and their swimming behaviors remains unexplored; indeed, warming could reverse 'lazy larvae syndrome.' This project uses a combination of manipulative laboratory experiments, computer modeling, and a real case study to examine whether the impacts of ocean warming and acidification on individual larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea. The project will tightly couple research with undergraduate education at Western Washington University, a primarily undergraduate university, by employing student researchers, incorporating materials into undergraduate courses, and pairing marine science student interns with art student interns to develop art projects aimed at communicating the effects of climate change to public audiences\nAs studies of the effects of climate stress in the marine environment progress, impacts on individual-level performance must be placed in a larger ecological context. While future climate-induced circulation changes certainly will affect larval dispersal, the effects of climate-change stressors on individual larval traits alone may have equally important impacts, significantly altering larval transport and, ultimately, species distribution. This study will experimentally examine the relationship between combined climate stressors (warming and acidification) on planktonic larval duration, morphology, and swimming behavior; create models to generate testable hypotheses about the effects of these factors on larval dispersal that can be applied across systems; and, finally, use a bio-physically coupled larval transport model to examine whether climate-impacted larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea.
attribute NC_GLOBAL projects_0_end_date String 2018-08
attribute NC_GLOBAL projects_0_geolocation String Coastal Pacific, USA
attribute NC_GLOBAL projects_0_name String RUI: Will climate change cause 'lazy larvae'? Effects of climate stressors on larval behavior and dispersal
attribute NC_GLOBAL projects_0_project_nid String 684167
attribute NC_GLOBAL projects_0_start_date String 2015-09
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Lipid analysis data collected from a laboratory experiment to investigate the grazing and physiological effects of ocean acidification on sand dollar larvae (Dendraster excentricus), July 2017.
attribute NC_GLOBAL title String [Lipid_Dendraster_OA_Expt2017] - Lipid analysis data from experiment on grazing and physiological effects of ocean acidification on sand dollar larvae (Dendraster excentricus), July 2017 (RUI: Will climate change cause 'lazy larvae'? Effects of climate stressors on larval behavior and dispersal)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable temp_treatment byte
attribute temp_treatment _FillValue byte 127
attribute temp_treatment actual_range byte 12, 17
attribute temp_treatment bcodmo_name String treatment
attribute temp_treatment description String The temperature condition of the water larvae were reared within
attribute temp_treatment long_name String Temp Treatment
attribute temp_treatment units String degrees Celsius
variable pH_treatment String
attribute pH_treatment bcodmo_name String treatment
attribute pH_treatment description String The pH condition of the water larvae were reared within: L = treatment of 400ppm; M = treatment of 800pm; H = treatment of 1500ppm
attribute pH_treatment long_name String P H Treatment
attribute pH_treatment units String unitless
variable jar_replicate byte
attribute jar_replicate _FillValue byte 127
attribute jar_replicate actual_range byte 1, 15
attribute jar_replicate bcodmo_name String replicate
attribute jar_replicate description String Replicate of the pH and temperature treatment combination. Four jars were maintained at each treatment.
attribute jar_replicate long_name String Jar Replicate
attribute jar_replicate units String unitless
variable lipid_presence String
attribute lipid_presence bcodmo_name String flag
attribute lipid_presence description String Is lipid present within measured larva? Yes or No
attribute lipid_presence long_name String Lipid Presence
attribute lipid_presence units String unitless
variable lipid_area double
attribute lipid_area _FillValue double NaN
attribute lipid_area actual_range double 0.0, 13250.238
attribute lipid_area bcodmo_name String surface_area
attribute lipid_area description String Area of larva's lipid content
attribute lipid_area long_name String Lipid Area
attribute lipid_area units String pixels per micron
variable stomach_area double
attribute stomach_area _FillValue double NaN
attribute stomach_area actual_range double 4692.515, 19999.86
attribute stomach_area bcodmo_name String surface_area
attribute stomach_area description String Area of larva's stomach
attribute stomach_area long_name String Stomach Area
attribute stomach_area units String pixels per micron
variable lipid_index float
attribute lipid_index _FillValue float NaN
attribute lipid_index actual_range float 0.0, 0.924
attribute lipid_index bcodmo_name String unknown
attribute lipid_index description String Area of larva's lipid content divided by area of larva's stomach
attribute lipid_index long_name String Lipid Index
attribute lipid_index units String unitless

 
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