BCO-DMO ERDDAP
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Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Activity concentrations of 234Th, 233Pa, 210Pb, and 7Be were measured by\ncounting the gamma decay energies at 63.5 keV, 312 keV, 46.5 keV, and 477.6\nkeV, respectively, on a Canberra ultrahigh purity germanium well detector. The\n210Po activity was analyzed by liquid scintillation counting (Beckman Model\n8100 Liquid Scintillation Counter).\n \nConcentrations of total carbohydrate (TCHO) were determined by the TPTZ (2, 4,\n6-tripyridyl-s-triazine) method using glucose as the standard and [Hung and\nSantschi, 2001]. Protein content was determined using a modified Lowry protein\nassay, using bovine serum albumin as the standard (Pierce, Thermo\nScientific).\\u00a0\n \nElemental contents of carbon (C) and nitrogen (N), were determined by a Perkin\nElmer CHN 2400 analyzer, using cysteine (29.99% C, 11.67% N) as a\nstandard.\\u00a0\n \nIn order to examine the specific binding ligands to five different\nradionuclides in the marine colloids and diatom culture-derived EPS,\nradiolabeled biopolymers (E1, E3, C9 and C11; suitable due to their high OC\ncontent and available sample amount) were subjected to Isoelectric Focusing\n(IEF) separation with a Gel Electrophoresis apparatus (Amersham Biosciences,\nMultiphor Electrophoresis System). Briefly, radiolabeled biopolymers and a 140\n\\u03bcL of rehydration solution were loaded onto an IPG strip (GE Healthcare\nImmobiline\\u2122 Drystrip, pH 3\\u201310, 11 cm) and were re-swelled overnight.\nAfterwards, the strip was loaded into the device for isoelectric focusing for\n17.5 h. The strip was then cut into eleven 1 cm-pieces and followed by 1% SDS\nextraction overnight. Activity concentrations of the five radionuclides in\neach fraction  \n were subsequently analyzed. Due to the limited amount of each strip\nfraction, only selected chemical components (TCHO, Proteins and Fe) in\nindividual fractions were characterized by methods described above. To avoid\nany interference from any background contamination, SDS extractants underwent\ndiafiltration (desalting) using 1 kDa cutoff Microsep\\u2122 centrifugal\ndevices (Pall Life Sciences) using ultra pure Milli-Q water for at least three\ntimes.\n \n\\u00a0
attribute NC_GLOBAL awards_0_award_nid String 735995
attribute NC_GLOBAL awards_0_award_number String OCE-1356453
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1356453 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Henrietta N Edmonds
attribute NC_GLOBAL awards_0_program_manager_nid String 51517
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String The partition coefficients of the radionuclide into colloidal organic matter, logKc values. \n  PI: Peter H. Santschi \n  Version: 2019-04-11
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3  19 Dec 2019
attribute NC_GLOBAL date_created String 2019-04-11T15:30:02Z
attribute NC_GLOBAL date_modified String 2019-04-11T19:55:18Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.764780.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/764780 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String CHN_EA
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Elemental contents of carbon (C) and nitrogen (N), were determined by a Perkin Elmer CHN 2400 analyzer, using cysteine (29.99% C, 11.67% N) as a standard.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 764856
attribute NC_GLOBAL instruments_0_description String A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and  nitrogen content in organic and other types of materials, including  solids, liquids, volatile, and viscous samples.
attribute NC_GLOBAL instruments_0_instrument_name String CHN Elemental Analyzer
attribute NC_GLOBAL instruments_0_instrument_nid String 625
attribute NC_GLOBAL instruments_0_supplied_name String Perkin Elmer CHN 2400 analyzer
attribute NC_GLOBAL instruments_1_dataset_instrument_description String In order to examine the specific binding ligands to five different radionuclides in the marine colloids and diatom culture-derived EPS, radiolabeled biopolymers (E1, E3, C9 and C11; suitable due to their high OC content and available sample amount) were subjected to Isoelectric Focusing (IEF) separation with a Gel Electrophoresis apparatus (Amersham Biosciences, Multiphor Electrophoresis System).
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 764857
attribute NC_GLOBAL instruments_1_description String General term for an apparatus used in clinical and research laboratories to separate charged colloidal particles (or molecules) of varying size through a medium by applying an electric field.
attribute NC_GLOBAL instruments_1_instrument_name String Electrophoresis Chamber
attribute NC_GLOBAL instruments_1_instrument_nid String 471592
attribute NC_GLOBAL instruments_1_supplied_name String Amersham Biosciences, Multiphor Electrophoresis System
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, calculation, calculation_type, chemical, compound, compound_for_ratio, data, dataset, dmo, erddap, log, LogK, LogK_radionuclide, management, oceanography, office, pcnt, preliminary, radionuclide, ratio, ratio_to_OC_pcnt, type
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/764780/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/764780 (external link)
attribute NC_GLOBAL param_mapping String {'764780': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/764780/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Texas A&M, Galveston
attribute NC_GLOBAL people_0_affiliation_acronym String TAMUG
attribute NC_GLOBAL people_0_person_name String Peter Santschi
attribute NC_GLOBAL people_0_person_nid String 735998
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Texas A&M, Galveston
attribute NC_GLOBAL people_1_affiliation_acronym String TAMUG
attribute NC_GLOBAL people_1_person_name String Antonietta Quigg
attribute NC_GLOBAL people_1_person_nid String 736000
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Texas A&M, Galveston
attribute NC_GLOBAL people_2_affiliation_acronym String TAMUG
attribute NC_GLOBAL people_2_person_name String Kathleen Schwehr
attribute NC_GLOBAL people_2_person_nid String 736002
attribute NC_GLOBAL people_2_role String Co-Principal Investigator
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String Texas A&M, Galveston
attribute NC_GLOBAL people_3_affiliation_acronym String TAMUG
attribute NC_GLOBAL people_3_person_name String Chen Xu
attribute NC_GLOBAL people_3_person_nid String 736004
attribute NC_GLOBAL people_3_role String Co-Principal Investigator
attribute NC_GLOBAL people_3_role_type String originator
attribute NC_GLOBAL people_4_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_4_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_4_person_name String Mathew Biddle
attribute NC_GLOBAL people_4_person_nid String 708682
attribute NC_GLOBAL people_4_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_4_role_type String related
attribute NC_GLOBAL project String Biopolymers for radionuclides
attribute NC_GLOBAL projects_0_acronym String Biopolymers for radionuclides
attribute NC_GLOBAL projects_0_description String NSF Award Abstract:\nParticle-associated natural radioisotopes are transported to the ocean floor mostly via silica and carbonate ballasted particles, allowing their use as tracers for particle transport. Th(IV), Pa (IV,V), Po(IV), Pb(II) and Be(II) radionuclides are important proxies in oceanographic investigations, used for tracing particle and colloid cycling, estimating export fluxes of particulate organic carbon, tracing air-sea exchange, paleoproductivity, and/or ocean circulation in paleoceanographic studies. Even though tracer approaches are considered routine, there are cases where data interpretation or validity has become controversial, largely due to uncertainties about inorganic proxies and organic carrier molecules. Recent studies showed that cleaned diatom frustules and pure silica particles, sorb natural radionuclides to a much lower extent (by 1-2 orders of magnitude) than whole diatom cells (with or without shells). Phytoplankton that build siliceous or calcareous shells, such as the diatoms and coccolithophores, are assembled via bio-mineralization processes using biopolymers as nanoscale templates. These templates could serve as possible carriers for radionuclides and stable metals.\nIn this project, a research team at the Texas A & M University at Galveston hypothesize that radionuclide sorption is controlled by selective biopolymers that are associated with biogenic opal (diatoms), CaCO3 (coccolithophores) and the attached exopolymeric substances (EPS), rather than to pure mineral phase. To pursue this idea, the major objectives of their research will include separation, identification and molecular-level characterization of the individual biopolymers (e.g., polysaccharides, uronic acids, proteins, hydroquinones, hydroxamate siderophores, etc.) that are responsible for binding different radionuclides (Th, Pa, Pb, Po and Be) attached to cells or in the matrix of biogenic opal or CaCO3 as well as attached EPS mixture, in laboratory grown diatom and coccolithophore cultures. Laboratory-scale radiolabeling experiments will be conducted, and different separation techniques and characterization techniques will be applied.\nIntellectual Merit : It is expected that this study will help elucidate the molecular basis of the templated growth of diatoms and coccoliths, EPS and their role in scavenging natural radionuclides in the ocean, and help resolve debates on the oceanographic tracer applications of different natural radioisotopes (230,234Th, 231Pa, 210Po, 210Pb and 7,10Be). The proposed interdisciplinary research project will require instrumental approaches for molecular-level characterization of these radionuclides associated carrier molecules.\nBroader Impacts: The results of this study will be relevant for understanding biologically mediated ocean scavenging of radionuclides by diatoms and coccoliths which is important for carbon cycling in the ocean, and will contribute to improved interpretation of data obtained by field studies especially through the GEOTRACES program. This new program will enhance training programs at TAMUG for postdocs, graduate and undergraduate students. Lastly, results will be integrated in college courses and out-reach activities at Texas A&M University, including NSF-REU, Sea Camp, Elder Hostel and exhibits at the local science fair and interaction with its after-school program engaging Grade 9-12 students from groups traditionally underrepresented.
attribute NC_GLOBAL projects_0_end_date String 2018-02
attribute NC_GLOBAL projects_0_name String Biopolymers as carrier phases for selected natural radionuclides (of Th, Pa, Pb, Po, Be) in diatoms and coccolithophores
attribute NC_GLOBAL projects_0_project_nid String 735996
attribute NC_GLOBAL projects_0_start_date String 2014-03
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String To study the binding mechanisms of radionuclides to organic moieties in colloidal organic matter (COM),marine colloids (1 kDa\\u20130.2 \\u03bcm) were isolated by cross-flow ultrafiltration from seawater of the west Pacific Ocean and the northern Gulf of Mexico. For the same purpose, exopolymeric substances (EPS) produced by laboratory cultured diatoms were collected as well. In our study areas, colloidal organic carbon (COC) concentrations ranged from 6.5 to 202 \\u03bcg-C/L in the Pacific Ocean, and were 808 \\u03bcg-C/L in the Gulf of Mexico. The COM compositions (organic carbon, organic nitrogen, proteins, total hydrolysable amino acids, total polysaccharides, uronic acids, hydroxamate siderophores, hydroquinone) were quantified to examine the relationships between partition coefficients (Kc) of five different radionuclides, 234Th, 233Pa, 210Pb, 210Po and 7Be, and concentration ratios to COC of individual chelating biomolecules that could potentially act as a chelating moiety. The range of partition coefficients (Kc, reported as logKc) of radionuclides between water and the different colloidal materials was 5.12 to 5.85 for 234Th, 5.19 to 6.01 for 233Pa, 4.21 to 4.85 for 210Pb, 4.87 to 5.68 for 210Po, and 4.49 to 4.92 for 7Be, similar to values previously reported for lab and field determinations under different particle concentrations. While any relationship obtained between Kc and abundance of specific moieties could not be taken as proving the existence of colloidal organic binding ligands for the different radionuclides, it could suggest possible organic moieties involved in the scavenging of these natural radionuclides. Together with results from isoelectric focusing of radiolabeled COM, we conclude that binding to different biomolecules is nuclide-specific, with colloidal hydroxamate siderophoric moieties being important for the binding of Th and Pa radionuclides. Hydroquinones/ quinone (HQ/Q) facilitated redox and chelation reactions seem to be involved in the binding of Pa and Be. However, the actual mechanisms are not clear. Individual amino acids, proteins, total polysaccharides and uronic acids did not yield significant relationships with logKc values of the different radionuclides. Nonetheless, our results provide new insights into the relative importance of different potential ligand moieties in COM in the binding and possible scavenging of specific radionuclides in the ocean.
attribute NC_GLOBAL title String [Partition_coefficients_for_COC] - Partition coefficients of the radionuclide into colloidal organic matter from seawater of the west Pacific Ocean and the northern Gulf of Mexico (Biopolymers as carrier phases for selected natural radionuclides (of Th, Pa, Pb, Po, Be) in diatoms and coccolithophores)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable compound_for_ratio String
attribute compound_for_ratio bcodmo_name String unknown
attribute compound_for_ratio description String compound used in the ratio for ratio_to_OC_pcnt
attribute compound_for_ratio long_name String Compound For Ratio
attribute compound_for_ratio units String unitless
variable ratio_to_OC_pcnt float
attribute ratio_to_OC_pcnt _FillValue float NaN
attribute ratio_to_OC_pcnt actual_range float 0.02, 2.04
attribute ratio_to_OC_pcnt bcodmo_name String unknown
attribute ratio_to_OC_pcnt description String Carbon normalized ratio of the compound in compound_for_ratio repreented as a percent
attribute ratio_to_OC_pcnt long_name String Ratio To OC Pcnt
attribute ratio_to_OC_pcnt units String unitless (percent)
variable LogK_radionuclide String
attribute LogK_radionuclide bcodmo_name String unknown
attribute LogK_radionuclide description String the radionuclide for the partition coefficient
attribute LogK_radionuclide long_name String Log K Radionuclide
attribute LogK_radionuclide units String unitless
variable LogK float
attribute LogK _FillValue float NaN
attribute LogK actual_range float 4.49, 6.01
attribute LogK bcodmo_name String unknown
attribute LogK description String partition coefficient of the radionuclide in LogK_radionuclide
attribute LogK long_name String Log K
attribute LogK units String logKc
variable calculation_type String
attribute calculation_type bcodmo_name String unknown
attribute calculation_type description String the procedure for calculating LogK
attribute calculation_type long_name String Calculation Type
attribute calculation_type units String unitless

 
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