BCO-DMO ERDDAP
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Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Three CO2 concentrations were tested: 410 ppm, 750 ppm, and 1000 ppm\nrespectively. For each CO2 concentration, four temperatures were tested: 15\ndegrees-C, 20 degrees-C, 25 degrees-C, and 30 degrees-C. Within each\ntemperature, three light levels were tested: a sub-optimum light (SOL)\nintensity of 60 umol photons \\u00b7 m-2 \\u00b7 s-1, an optimum light (OL)\nintensity of 400 umol photons \\u00b7 m-2 \\u00b7 s-1 and an extreme light (EL)\nintensity of 800 umol photons \\u00b7 m-2 \\u00b7 s-1. All lights were set at a\n12 h day: 12 h dark cycle. For logistical reasons, experiments were partially\nconducted in series, with all light treatments at two temperatures (either 15\ndegrees-C and 25 degrees-C or 20 degrees-C and 30 degrees-C) running\nsimultaneously. This was repeated for each CO2 concentration.\n \nExperiments were conducted in Multicultivator MC-1000 OD units (Photon Systems\nInstruments, Drasov, Czech Republic). Each unit consists of eight 85 ml test-\ntubes immersed in a thermostated water bath, each independently illuminated by\nan array of cool white LEDs set at specific intensity and timing. A 0.2um\nfiltered CO2-air mix (Praxair Distribution Inc.) was bubbled through sterile\nartificial seawater, and the humidified gas mix was supplied to each tube via\ngentle sparging through a 2um stainless steel diffuser. Flow rates were\ngradually increased over the course of the incubation to compensate for the\nDIC uptake of actively growing cells, and ranged from <0.04 Liters per minute\n(LPM) at the start of the incubations to 0.08 LPM in each tube after 2 days.\nFor each CO2 and temperature level, replication was achieved by incubating\nthree tubes at sub-optimum light intensities, two tubes at optimum light\nintensity, and three tubes at extreme light intensities. Each experiment was\nsplit into two phases: An acclimation phase spanning 4 days, was used to\nacclimate cultures to their new environment. Pre-acclimated, exponentially-\ngrowing cultures were then inoculated into fresh media and incubated through a\n3-day experimental phase during which assessments of growth, photophysiology,\nand nutrient cycling were carried out daily. All sampling started 5 hours into\nthe daily light cycle to minimize the effects of diurnal cycles.\n \nExperiments were conducted with artificial seawater (ASW) prepared using\npreviously described methods (Kester et. al 1967), and enriched with nitrate\n(NO3), phosphate (PO4), silicic acid (Si[OH]4), at levels ensuring that the\ncultures would remain nutrient-replete over the course of the experiment.\nTrace metals and vitamins were added as in f/2 (Guillard 1975). The expected\nDIC concentration and pH of the growth media was determined for the different\npCO2 and temperatures using the CO2SYS calculator (Pierrot et al. 2006), with\nconstants from Mehrbach et al. (1973, refit by Dickson & Millero 1987), and\ninputs of temperature, salinity, total alkalinity (2376.5 umol \\u00b7 kg-1),\npCO2, phosphate, and silicic acid. DIC levels in ASW at the start of each\nphase of the experiments were manipulated by the addition of NaHCO3, and was\nthen maintained by bubbling a CO2-Air mix through the cultures over the course\nof the experiments. The pH of the growth media was measured\nspectrophometrically using the m-cresol purple method (Dickson 1993), and\nadjusted using 0.1N HCl or 0.1M NaOH. The media was distributed into 75 ml\naliquots and each aliquot was inoculated with 5 ml of the T. pseudonana CCMP\n1014 (TP1014) stock culture at the start of the experiments.\n \npH measurements:  \n Three ml samples were collected daily to assess pH. The pH was measured with\na spectrophotometer (Genesys 10SVIS) using the indicator dye m-cresol purple\n(Sigma Aldrich) at 25 degrees-C. The absorbance was measured at 730 nm, 578\nnm, and 434 nm before and after dye addition (Clayton & Byrne 1993, Fangue et\nal. 2010). A TRIS buffer solution in synthetic seawater with known pH,\nsupplied by A. Dickson (Scripps Institution of Oceanography, USA) was used to\ncalibrate the dye.
attribute NC_GLOBAL awards_0_award_nid String 654346
attribute NC_GLOBAL awards_0_award_number String OCE-1538602
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1538602 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_0_program_manager_nid String 50446
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Series 3A-1: Multiple stressor experiments on T. pseudonana (CCMP1014): pH \n   PI: U. Passow, N. D'Souza  (UCSB), E. Laws (LSU) \n   version date: 2019-06-17
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3  19 Dec 2019
attribute NC_GLOBAL dataset_current_state String Final and no updates
attribute NC_GLOBAL date_created String 2019-06-19T18:38:12Z
attribute NC_GLOBAL date_modified String 2020-06-29T12:52:17Z
attribute NC_GLOBAL defaultDataQuery String &amp;time&lt;now
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.771304.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/771304 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Spectrophotometer
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to measure sample pH.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 771317
attribute NC_GLOBAL instruments_0_description String An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB20/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Spectrophotometer
attribute NC_GLOBAL instruments_0_instrument_nid String 707
attribute NC_GLOBAL instruments_0_supplied_name String Genesys 10SVIS spectrophotometer
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Used for incubation of TP1014 cultures.
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 771312
attribute NC_GLOBAL instruments_1_description String An instrument used for the purpose of culturing small cells such as algae or bacteria. May provide temperature and light control and bubbled gas introduction.
attribute NC_GLOBAL instruments_1_instrument_name String Cell Cultivator
attribute NC_GLOBAL instruments_1_instrument_nid String 714540
attribute NC_GLOBAL instruments_1_supplied_name String Multicultivator MC-1000 OD (Photon Systems Instruments, Drasov, Czech Republic)
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, carbon, carbon dioxide, chemical, co2, data, dataset, day, dioxide, dmo, EL_A, EL_B, EL_C, erddap, management, oceanography, office, OL_A, OL_B, phase, preliminary, replicate, sol, SOL_A, SOL_B, SOL_C, Temp, temperature
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/771304/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/771304 (external link)
attribute NC_GLOBAL param_mapping String {'771304': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/771304/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of California-Santa Barbara
attribute NC_GLOBAL people_0_affiliation_acronym String UCSB-MSI
attribute NC_GLOBAL people_0_person_name String Uta Passow
attribute NC_GLOBAL people_0_person_nid String 51317
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Louisiana State University
attribute NC_GLOBAL people_1_affiliation_acronym String LSU-SC&E
attribute NC_GLOBAL people_1_person_name String Dr Edward Laws
attribute NC_GLOBAL people_1_person_nid String 50767
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String University of California-Santa Barbara
attribute NC_GLOBAL people_2_affiliation_acronym String UCSB-MSI
attribute NC_GLOBAL people_2_person_name String Nigel D'Souza
attribute NC_GLOBAL people_2_person_nid String 748936
attribute NC_GLOBAL people_2_role String Scientist
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String University of California-Santa Barbara
attribute NC_GLOBAL people_3_affiliation_acronym String UCSB-MSI
attribute NC_GLOBAL people_3_person_name String Nigel D'Souza
attribute NC_GLOBAL people_3_person_nid String 748936
attribute NC_GLOBAL people_3_role String Contact
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL people_4_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_4_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_4_person_name String Nancy Copley
attribute NC_GLOBAL people_4_person_nid String 50396
attribute NC_GLOBAL people_4_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_4_role_type String related
attribute NC_GLOBAL project String Stressors on Marine Phytoplankton
attribute NC_GLOBAL projects_0_acronym String Stressors on Marine Phytoplankton
attribute NC_GLOBAL projects_0_description String The overarching goal of this project is to develop a framework for understanding the response of phytoplankton to multiple environmental stresses. Marine phytoplankton, which are tiny algae, produce as much oxygen as terrestrial plants and provide food, directly or indirectly, to all marine animals. Their productivity is thus important both for global elemental cycles of oxygen and carbon, as well as for the productivity of the ocean. Globally the productivity of marine phytoplankton appears to be changing, but while we have some understanding of the response of phytoplankton to shifts in one environmental parameter at a time, like temperature, there is very little knowledge of their response to simultaneous changes in several parameters. Increased atmospheric carbon dioxide concentrations result in both ocean acidification and increased surface water temperatures. The latter in turn leads to greater ocean stratification and associated changes in light exposure and nutrient availability for the plankton. Recently it has become apparent that the response of phytoplankton to simultaneous changes in these growth parameters is not additive. For example, the effect of ocean acidification may be severe at one temperature-light combination and negligible at another. The researchers of this project will carry out experiments that will provide a theoretical understanding of the relevant interactions so that the impact of climate change on marine phytoplankton can be predicted in an informed way. This project will engage high schools students through training of a teacher and the development of a teaching unit. Undergraduate and graduate students will work directly on the research. A cartoon journalist will create a cartoon story on the research results to translate the findings to a broader general public audience.\nEach phytoplankton species has the capability to acclimatize to changes in temperature, light, pCO2, and nutrient availability - at least within a finite range. However, the response of phytoplankton to multiple simultaneous stressors is frequently complex, because the effects on physiological responses are interactive. To date, no datasets exist for even a single species that could fully test the assumptions and implications of existing models of phytoplankton acclimation to multiple environmental stressors. The investigators will combine modeling analysis with laboratory experiments to investigate the combined influences of changes in pCO2, temperature, light, and nitrate availability on phytoplankton growth using cultures of open ocean and coastal diatom strains (Thalassiosira pseudonana) and an open ocean cyanobacteria species (Synechococcus sp.). The planned experiments represent ideal case studies of the complex and interactive effects of environmental conditions on organisms, and results will provide the basis for predictive modeling of the response of phytoplankton taxa to multiple environmental stresses.
attribute NC_GLOBAL projects_0_end_date String 2018-09
attribute NC_GLOBAL projects_0_name String Collaborative Research: Effects of multiple stressors on Marine Phytoplankton
attribute NC_GLOBAL projects_0_project_nid String 654347
attribute NC_GLOBAL projects_0_start_date String 2015-10
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL subsetVariables String Replicate
attribute NC_GLOBAL summary String The experiments were designed to test the combined effects of three CO2 concentrations, four temperatures, and three light intensities on growth and photophysiology of the diatom T. pseudonana CCMP1014 in a multifactorial design. This dataset contains measurements of pH made over the course of the experiments.
attribute NC_GLOBAL title String [Series 3A: pH] - Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) – pH measurements (Collaborative Research: Effects of multiple stressors on Marine Phytoplankton)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.5
variable Phase String
attribute Phase bcodmo_name String sample_descrip
attribute Phase description String Indicates whether the sample was collected during the acclimation phase or the experiment phase of the experiment.
attribute Phase long_name String Phase
attribute Phase units String unitless
variable CO2 short
attribute CO2 _FillValue short 32767
attribute CO2 actual_range short 410, 1000
attribute CO2 bcodmo_name String pCO2
attribute CO2 description String Indicates the concentration of CO2 in the CO2-Air mix that was bubbled through the samples over the course of the experiment
attribute CO2 long_name String CO2
attribute CO2 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PCO2C101/ (external link)
attribute CO2 units String parts per million (ppm)
variable Temp byte
attribute Temp _FillValue byte 127
attribute Temp actual_range byte 15, 30
attribute Temp bcodmo_name String temperature
attribute Temp description String Indicates the temperature at which the samples were incubated.
attribute Temp long_name String Temperature
attribute Temp nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/ (external link)
attribute Temp units String degrees Celsius
variable Day String
attribute Day bcodmo_name String days
attribute Day description String Indicates the timepoint (day) of sampling. D0 = day 0; D1 = day 1; etc.
attribute Day long_name String Day
attribute Day units String unitless
variable Replicate String
attribute Replicate bcodmo_name String replicate
attribute Replicate description String Indicates replication within a treatment. \"NA\" indicates \"not applicable\"
attribute Replicate long_name String Replicate
attribute Replicate units String unitless
variable SOL_A float
attribute SOL_A _FillValue float NaN
attribute SOL_A actual_range float 7.63, 8.07
attribute SOL_A bcodmo_name String pH
attribute SOL_A description String pH measurements in replicate A incubated at sub optimum light (SOL)
attribute SOL_A long_name String SOL A
attribute SOL_A nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/ (external link)
attribute SOL_A units String unitless; pH scale
variable SOL_B float
attribute SOL_B _FillValue float NaN
attribute SOL_B actual_range float 7.62, 8.07
attribute SOL_B bcodmo_name String pH
attribute SOL_B description String pH measurements in replicate B incubated at sub optimum light (SOL)
attribute SOL_B long_name String SOL B
attribute SOL_B nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/ (external link)
attribute SOL_B units String unitless; pH scale
variable SOL_C float
attribute SOL_C _FillValue float NaN
attribute SOL_C actual_range float 7.63, 8.07
attribute SOL_C bcodmo_name String pH
attribute SOL_C description String pH measurements in replicate C incubated at sub optimum light (SOL)
attribute SOL_C long_name String SOL C
attribute SOL_C nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/ (external link)
attribute SOL_C units String unitless; pH scale
variable OL_A float
attribute OL_A _FillValue float NaN
attribute OL_A actual_range float 7.62, 8.24
attribute OL_A bcodmo_name String pH
attribute OL_A description String pH measurements in replicate A incubated at optimum light (OL)
attribute OL_A long_name String OL A
attribute OL_A nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/ (external link)
attribute OL_A units String unitless; pH scale
variable OL_B float
attribute OL_B _FillValue float NaN
attribute OL_B actual_range float 7.62, 8.18
attribute OL_B bcodmo_name String pH
attribute OL_B description String pH measurements in replicate B incubated at optimum light (OL)
attribute OL_B long_name String OL B
attribute OL_B nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/ (external link)
attribute OL_B units String unitless; pH scale
variable EL_A float
attribute EL_A _FillValue float NaN
attribute EL_A actual_range float 7.63, 8.3
attribute EL_A bcodmo_name String pH
attribute EL_A description String pH measurements in replicate A incubated at extreme light (EL)
attribute EL_A long_name String EL A
attribute EL_A nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/ (external link)
attribute EL_A units String unitless; pH scale
variable EL_B float
attribute EL_B _FillValue float NaN
attribute EL_B actual_range float 7.63, 8.35
attribute EL_B bcodmo_name String pH
attribute EL_B description String pH measurements in replicate B incubated at extreme light (EL)
attribute EL_B long_name String EL B
attribute EL_B nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/ (external link)
attribute EL_B units String unitless; pH scale
variable EL_C float
attribute EL_C _FillValue float NaN
attribute EL_C actual_range float 7.64, 8.3
attribute EL_C bcodmo_name String pH
attribute EL_C description String pH measurements in replicate C incubated at extreme light (EL)
attribute EL_C long_name String EL C
attribute EL_C nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/ (external link)
attribute EL_C units String unitless; pH scale

 
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