BCO-DMO ERDDAP
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Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Three CO2 concentrations were tested: 410 ppm, 750 ppm, and 1000 ppm\nrespectively. For each CO2 concentration, four temperatures were tested: 15\ndegrees-C, 20 degrees-C, 25 degrees-C, and 30 degrees-C. Within each\ntemperature, three light levels were tested: a sub-optimum light (SOL)\nintensity of 60 umol photons \\u00b7 m-2 \\u00b7 s-1, an optimum light (OL)\nintensity of 400 umol photons \\u00b7 m-2 \\u00b7 s-1 and an extreme light (EL)\nintensity of 800 umol photons \\u00b7 m-2 \\u00b7 s-1. All lights were set at a\n12 h day: 12 h dark cycle. For logistical reasons, experiments were partially\nconducted in series, with all light treatments at two temperatures (either 15\ndegrees-C and 25 degrees-C or 20 degrees-C and 30 degrees-C) running\nsimultaneously. This was repeated for each CO2 concentration.\n \nExperiments were conducted in Multicultivator MC-1000 OD units (Photon Systems\nInstruments, Drasov, Czech Republic). Each unit consists of eight 85 ml test-\ntubes immersed in a thermostated water bath, each independently illuminated by\nan array of cool white LEDs set at specific intensity and timing. A 0.2um\nfiltered CO2-air mix (Praxair Distribution Inc.) was bubbled through sterile\nartificial seawater, and the humidified gas mix was supplied to each tube via\ngentle sparging through a 2um stainless steel diffuser. Flow rates were\ngradually increased over the course of the incubation to compensate for the\nDIC uptake of actively growing cells, and ranged from <0.04 Liters per minute\n(LPM) at the start of the incubations to 0.08 LPM in each tube after 2 days.\nFor each CO2 and temperature level, replication was achieved by incubating\nthree tubes at sub-optimum light intensities, two tubes at optimum light\nintensity, and three tubes at extreme light intensities. Each experiment was\nsplit into two phases: An acclimation phase spanning 4 days, was used to\nacclimate cultures to their new environment. Pre-acclimated, exponentially-\ngrowing cultures were then inoculated into fresh media and incubated through a\n3-day experimental phase during which assessments of growth, photophysiology,\nand nutrient cycling were carried out daily. All sampling started 5 hours into\nthe daily light cycle to minimize the effects of diurnal cycles.\n \nExperiments were conducted with artificial seawater (ASW) prepared using\npreviously described methods (Kester et. al 1967), and enriched with nitrate\n(NO3), phosphate (PO4), silicic acid (Si[OH]4), at levels ensuring that the\ncultures would remain nutrient-replete over the course of the experiment.\nTrace metals and vitamins were added as in f/2 (Guillard 1975). The expected\nDIC concentration and pH of the growth media was determined for the different\npCO2 and temperatures using the CO2SYS calculator (Pierrot et al. 2006), with\nconstants from Mehrbach et al. (1973, refit by Dickson & Millero 1987), and\ninputs of temperature, salinity, total alkalinity (2376.5 umol \\u00b7 kg-1),\npCO2, phosphate, and silicic acid. DIC levels in ASW at the start of each\nphase of the experiments were manipulated by the addition of NaHCO3, and was\nthen maintained by bubbling a CO2-Air mix through the cultures over the course\nof the experiments. The pH of the growth media was measured\nspectrophometrically using the m-cresol purple method (Dickson 1993), and\nadjusted using 0.1N HCl or 0.1M NaOH. The media was distributed into 75 ml\naliquots and each aliquot was inoculated with 5 ml of the T. pseudonana CCMP\n1014 (TP1014) stock culture at the start of the experiments.\n \nOrganic Carbon and Nitrogen concentrations:  \n Samples were filtered onto pre-combusted GF/F filters, dried at 60 degrees\nC, and stored at room temperature until analyses of particulate organic carbon\n(POC), and particulate organic nitrogen (PON). Between 3 and > 10 mL were\nfiltered, with larger filtration volumes used on the final day of the\nexperiment. Samples were analyzed using an elemental analyzer (CEC 44OHA;\nControl Equipment). Samples where C or N concentrations were below instrument\ndetection limits were flagged.\n \nChlorophyll:  \n Daily subsamples from each treatment were filtered onto 0.45 \\u00b5m\npolycarbonate filters and stored at -20 degrees C. Filters were placed in 90%\nacetone (v/v) overnight at -20 degrees C, and the extracted chlorophyll was\nmeasured fluorometrically on a Turner 700 fluorometer (Strickland 1972).\nChlorophyll-a liquid standards in 90% acetone (Turner Designs Inc.), and\nadjustable solid secondary standards (Turner Designs Inc. P/N 8000-952) were\nused for calibrations, and to calculate the chlorophyll content of the samples\n(Column O)
attribute NC_GLOBAL awards_0_award_nid String 654346
attribute NC_GLOBAL awards_0_award_number String OCE-1538602
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1538602 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_0_program_manager_nid String 50446
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Series 3A-4: Multiple stressor experiments on T. pseudonana (CCMP1014): chlorophyll, particulate organic carbon (POC) and particulate organic nitrogen (PON) \n   PI: U. Passow, N. D'Souza  (UCSB), E. Laws (LSU) \n   version date: 2019-06-17
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3  19 Dec 2019
attribute NC_GLOBAL dataset_current_state String Final and no updates
attribute NC_GLOBAL date_created String 2019-06-20T18:36:54Z
attribute NC_GLOBAL date_modified String 2020-06-29T13:05:31Z
attribute NC_GLOBAL defaultDataQuery String &amp;time&lt;now
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.771594.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/771594 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Fluorometer
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used for fluorometric analyses of extracted chlorophyll.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 771604
attribute NC_GLOBAL instruments_0_description String A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/113/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Fluorometer
attribute NC_GLOBAL instruments_0_instrument_nid String 484
attribute NC_GLOBAL instruments_0_supplied_name String Turner 700 fluorometer
attribute NC_GLOBAL instruments_1_acronym String CHN_EA
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Used for analysis of total organic carbon content.
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 771606
attribute NC_GLOBAL instruments_1_description String A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and  nitrogen content in organic and other types of materials, including  solids, liquids, volatile, and viscous samples.
attribute NC_GLOBAL instruments_1_instrument_name String CHN Elemental Analyzer
attribute NC_GLOBAL instruments_1_instrument_nid String 625
attribute NC_GLOBAL instruments_1_supplied_name String Elemental analyzer (CEC 44OHA; Control Equipment)
attribute NC_GLOBAL instruments_2_dataset_instrument_description String Used for incubation of TP1014 cultures.
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 771602
attribute NC_GLOBAL instruments_2_description String An instrument used for the purpose of culturing small cells such as algae or bacteria. May provide temperature and light control and bubbled gas introduction.
attribute NC_GLOBAL instruments_2_instrument_name String Cell Cultivator
attribute NC_GLOBAL instruments_2_instrument_nid String 714540
attribute NC_GLOBAL instruments_2_supplied_name String Multicultivator MC-1000 OD (Qubit Systems)
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, C_detect_lim, C_ug, carbon, carbon dioxide, chemical, chemistry, Chl_a_pg_L, chlorophyll, co2, concentration, concentration_of_chlorophyll_in_sea_water, data, dataset, day, detect, dioxide, dmo, earth, Earth Science > Oceans > Ocean Chemistry > Chlorophyll, erddap, filtered, flags, label, light, lim, management, N_detect_lim, N_ug, ocean, oceanography, oceans, office, phase, preliminary, reference, Reference_Label, replicate, science, sea, seawater, Temp, temperature, volume, Volume_filtered, water
attribute NC_GLOBAL keywords_vocabulary String GCMD Science Keywords
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/771594/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/771594 (external link)
attribute NC_GLOBAL param_mapping String {'771594': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/771594/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of California-Santa Barbara
attribute NC_GLOBAL people_0_affiliation_acronym String UCSB-MSI
attribute NC_GLOBAL people_0_person_name String Uta Passow
attribute NC_GLOBAL people_0_person_nid String 51317
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Louisiana State University
attribute NC_GLOBAL people_1_affiliation_acronym String LSU-SC&E
attribute NC_GLOBAL people_1_person_name String Dr Edward Laws
attribute NC_GLOBAL people_1_person_nid String 50767
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String University of California-Santa Barbara
attribute NC_GLOBAL people_2_affiliation_acronym String UCSB-MSI
attribute NC_GLOBAL people_2_person_name String Nigel D'Souza
attribute NC_GLOBAL people_2_person_nid String 748936
attribute NC_GLOBAL people_2_role String Scientist
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String University of California-Santa Barbara
attribute NC_GLOBAL people_3_affiliation_acronym String UCSB-MSI
attribute NC_GLOBAL people_3_person_name String Nigel D'Souza
attribute NC_GLOBAL people_3_person_nid String 748936
attribute NC_GLOBAL people_3_role String Contact
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL people_4_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_4_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_4_person_name String Nancy Copley
attribute NC_GLOBAL people_4_person_nid String 50396
attribute NC_GLOBAL people_4_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_4_role_type String related
attribute NC_GLOBAL project String Stressors on Marine Phytoplankton
attribute NC_GLOBAL projects_0_acronym String Stressors on Marine Phytoplankton
attribute NC_GLOBAL projects_0_description String The overarching goal of this project is to develop a framework for understanding the response of phytoplankton to multiple environmental stresses. Marine phytoplankton, which are tiny algae, produce as much oxygen as terrestrial plants and provide food, directly or indirectly, to all marine animals. Their productivity is thus important both for global elemental cycles of oxygen and carbon, as well as for the productivity of the ocean. Globally the productivity of marine phytoplankton appears to be changing, but while we have some understanding of the response of phytoplankton to shifts in one environmental parameter at a time, like temperature, there is very little knowledge of their response to simultaneous changes in several parameters. Increased atmospheric carbon dioxide concentrations result in both ocean acidification and increased surface water temperatures. The latter in turn leads to greater ocean stratification and associated changes in light exposure and nutrient availability for the plankton. Recently it has become apparent that the response of phytoplankton to simultaneous changes in these growth parameters is not additive. For example, the effect of ocean acidification may be severe at one temperature-light combination and negligible at another. The researchers of this project will carry out experiments that will provide a theoretical understanding of the relevant interactions so that the impact of climate change on marine phytoplankton can be predicted in an informed way. This project will engage high schools students through training of a teacher and the development of a teaching unit. Undergraduate and graduate students will work directly on the research. A cartoon journalist will create a cartoon story on the research results to translate the findings to a broader general public audience.\nEach phytoplankton species has the capability to acclimatize to changes in temperature, light, pCO2, and nutrient availability - at least within a finite range. However, the response of phytoplankton to multiple simultaneous stressors is frequently complex, because the effects on physiological responses are interactive. To date, no datasets exist for even a single species that could fully test the assumptions and implications of existing models of phytoplankton acclimation to multiple environmental stressors. The investigators will combine modeling analysis with laboratory experiments to investigate the combined influences of changes in pCO2, temperature, light, and nitrate availability on phytoplankton growth using cultures of open ocean and coastal diatom strains (Thalassiosira pseudonana) and an open ocean cyanobacteria species (Synechococcus sp.). The planned experiments represent ideal case studies of the complex and interactive effects of environmental conditions on organisms, and results will provide the basis for predictive modeling of the response of phytoplankton taxa to multiple environmental stresses.
attribute NC_GLOBAL projects_0_end_date String 2018-09
attribute NC_GLOBAL projects_0_name String Collaborative Research: Effects of multiple stressors on Marine Phytoplankton
attribute NC_GLOBAL projects_0_project_nid String 654347
attribute NC_GLOBAL projects_0_start_date String 2015-10
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String The experiments were designed to test the combined effects of CO2, temperatures, and light on growth and photophysiology of the diatom T. pseudonana CCMP1014 in a multifactorial design. This dataset contains measurements of extracted chlorophyll, particulate organic carbon (POC), and particulate organic nitrogen (PON) made over the course of the experiments.
attribute NC_GLOBAL title String [Series 3A: POC, PON, Chl a] - Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) - Chlorophyll, particulate organic carbon and particulate organic nitrogen. (Collaborative Research: Effects of multiple stressors on Marine Phytoplankton)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.5
variable Phase String
attribute Phase bcodmo_name String sample_descrip
attribute Phase description String Indicates whether the sample was collected during the acclimation phase or the experiment phase of the experiment.
attribute Phase long_name String Phase
attribute Phase units String unitless
variable CO2 short
attribute CO2 _FillValue short 32767
attribute CO2 actual_range short 410, 1000
attribute CO2 bcodmo_name String pCO2
attribute CO2 description String concentration of CO2 in the CO2-Air mix that was bubbled through the samples over the course of the experiment
attribute CO2 long_name String CO2
attribute CO2 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PCO2C101/ (external link)
attribute CO2 units String parts per million (ppm)
variable Temp byte
attribute Temp _FillValue byte 127
attribute Temp actual_range byte 15, 30
attribute Temp bcodmo_name String temperature
attribute Temp description String temperature at which the samples were incubated
attribute Temp long_name String Temperature
attribute Temp nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/ (external link)
attribute Temp units String degrees Celsius
variable Day String
attribute Day bcodmo_name String days
attribute Day description String timepoint (day) of sampling. D0 = day 0; D1 = day 1; etc.
attribute Day long_name String Day
attribute Day units String unitless
variable Light short
attribute Light _FillValue short 32767
attribute Light actual_range short 60, 800
attribute Light bcodmo_name String irradiance
attribute Light description String light intensity
attribute Light long_name String Light
attribute Light nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/VSRW/ (external link)
attribute Light units String micromol photons/meter^2/second (umol photons · m-2 · s-1)
variable Replicate byte
attribute Replicate _FillValue byte 127
attribute Replicate actual_range byte 1, 3
attribute Replicate bcodmo_name String replicate
attribute Replicate description String replication within a treatment
attribute Replicate long_name String Replicate
attribute Replicate units String unitless
variable Reference_Label String
attribute Reference_Label bcodmo_name String sample
attribute Reference_Label description String Reference label (used internally for verifying sample identity)
attribute Reference_Label long_name String Reference Label
attribute Reference_Label nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute Reference_Label units String unitless
variable C_ug float
attribute C_ug _FillValue float NaN
attribute C_ug actual_range float 1.1, 3252.7
attribute C_ug bcodmo_name String POC
attribute C_ug description String Particulate organic carbon concentration in sample
attribute C_ug long_name String C Ug
attribute C_ug nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CORGCAP1/ (external link)
attribute C_ug units String micrograms/milliliter
variable N_ug float
attribute N_ug _FillValue float NaN
attribute N_ug actual_range float -1.8, 327.8
attribute N_ug bcodmo_name String PON
attribute N_ug description String Particulate organic nitrogen concentration in sample
attribute N_ug long_name String N Ug
attribute N_ug nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/MDMAP013/ (external link)
attribute N_ug units String micrograms/milliliter
variable Flags String
attribute Flags bcodmo_name String flag
attribute Flags description String Indicates if C or N levels were below detection limits (BDL) of the instrument
attribute Flags long_name String Flags
attribute Flags units String unitless
variable C_detect_lim float
attribute C_detect_lim _FillValue float NaN
attribute C_detect_lim actual_range float 0.9, 7.1
attribute C_detect_lim bcodmo_name String unknown
attribute C_detect_lim description String Instrument detection limit for particulate organic carbon concentration
attribute C_detect_lim long_name String C Detect Lim
attribute C_detect_lim units String micrograms/milliliter
variable N_detect_lim float
attribute N_detect_lim _FillValue float NaN
attribute N_detect_lim actual_range float 0.4, 4.24
attribute N_detect_lim bcodmo_name String unknown
attribute N_detect_lim description String Instrument detection limit for particulate organic carbon concentration
attribute N_detect_lim long_name String N Detect Lim
attribute N_detect_lim units String micrograms/milliliter
variable Volume_filtered float
attribute Volume_filtered _FillValue float NaN
attribute Volume_filtered actual_range float 2.25, 40.0
attribute Volume_filtered bcodmo_name String vol_filt
attribute Volume_filtered description String Volume of the sample that was filtered
attribute Volume_filtered long_name String Volume Filtered
attribute Volume_filtered units String milliliters
variable Chl_a_pg_L float
attribute Chl_a_pg_L _FillValue float NaN
attribute Chl_a_pg_L actual_range float 4.72, 1843.72
attribute Chl_a_pg_L bcodmo_name String chlorophyll a
attribute Chl_a_pg_L colorBarMaximum double 30.0
attribute Chl_a_pg_L colorBarMinimum double 0.03
attribute Chl_a_pg_L colorBarScale String Log
attribute Chl_a_pg_L description String Chlorophyll concentration
attribute Chl_a_pg_L long_name String Concentration Of Chlorophyll In Sea Water
attribute Chl_a_pg_L nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLHPP1/ (external link)
attribute Chl_a_pg_L units String picograms/liter

 
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