BCO-DMO ERDDAP
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Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Mussels (M. trossulus) were collected in May 2012 from Argyle Creek, San Juan\nIsland, WA (48.52\\u02da N, 123.01\\u02da W) and held in a mesh box submerged\nunder the dock at Friday Harbor Laboratories (FHL), San Juan Island, WA for up\nto 14 d. Mussels were placed in experimental mesocosms in the Ocean\nAcidification Experimental Laboratory (OAEL) at FHL as described in\nO\\u2019Donnell et al. (2013) and Timmins-Schiffman et al. (2012). Briefly,\nmanipulations of pH were made by bubbling CO2 into a 150 L temperature-\ncontrolled seawater reservoir, that supplied water to eight 3.5 L chambers at\na turnover rate of 50 ml min-1. Air was bubbled into the reservoir to maintain\n100% oxygen saturation and submersible pumps (model number P396, Annex Depot,\nSacramento, CA) provided mixing in the chambers at 3.8 L min-1. The bottom of\neach chamber was lined with autoclaved pebbles, collected from an FHL beach,\nto provide a substrate for byssal thread attachment. pH and temperature were\nmonitored continuously in each water reservoir with a Durafet pH and\ntemperature probe and the full carbonate chemistry of the system evaluated\nwith DIC and Total alkalinity measurements once during each trial. Mussels\nwere acclimated to their treatment temperatures in ambient pH (~7.8) over 9 d,\nramping temperature up no more than 2\\u02daC per day, and fed a maintenance\nlevel of Shellfish Diet 1800 (6 g l-1 day-1, Reed Mariculture, Campell, CA,\nUSA).\n \nThe twelve independent temperature x pCO2 treatments spanned the range of\nlocal marine conditions (Newcomb, 2015; George et al., 2019; temperature at\n10\\u02daC, 18\\u02daC, or 25\\u02daC and pCO2 at 400, 750, 1200, or 2500\n\\u00b5atm). Each mussel was trimmed of external byssus before placement in an\nexperimental treatment for 3 d, sufficient time to produce new mature byssal\nthreads (Bell & Gosline 1996) while minimizing the effect of treatment on\nmussel condition. Mussels were starved during the 3 d trials to minimize\nchanges in chamber water chemistry due to food addition and to reduce fouling.\nThree trials were conducted in succession to replicate treatments over time,\nincreasing sample size (n=8 x 3) for each temperature*pCO2 treatment.\n \nAt the end of each trial, mussels and the rocks to which they had attached\nwith byssal threads were removed from the chambers. The entire byssus was\ndissected from each mussel and stored air-dried for up to 20 days. Byssus was\nrehydrated in seawater prior to testing, a method that does not alter the\nmechanical properties of the byssal threads (Brazee, 2004). The number of\nbyssal threads each mussel produced was counted, and one thread was\nhaphazardly chosen for mechanical testing following the procedure of Bell &\nGosline (1996). Briefly, an individual thread was clamped with submersible\npneumatic grips on either end by holding the proximal byssal stem between\ncardstock with cyanoacrylate glue and affixing the distal plaque with attached\nrock to an aluminum T-bar with epoxy. An Instron 5565 tensometer (Norwood MA,\nUSA), extended the thread at a rate of 10 mm min-1 in a temperature-controlled\nwater bath (3130-100 BioPuls Bath, Instron, Norwood, MA, USA) until failure.\nThe tensometer measured force (\\u00b110-3 N) and extension (\\u00b1 10-3 mm) at\n10 Hz. Tests were performed in seawater with a pH of 7.8 and the relevant\ntreatment temperature.\n \nPull to failure mechanical tests provided estimates of thread breaking force,\nyield force, extensibility, initial stiffness and failure location (Bell &\nGosline 1996). Yield, due to quasi-plastic deformation in the distal region,\nwas identified as the point where the initial slope of the force-extension\ncurve decreased by 40%. Extensibility was calculated by dividing thread\nextension at failure by initial length and initial stiffness was determined\nfrom the initial slope of the force extension curve. The location of failure\n(proximal, plaque, and/or distal region) was noted and threads were retested\nto quantify the breaking force of each remaining region. Tests that broke at\nthe grips were considered underestimates and were discarded.\n \nThe cross-sectional area of the proximal region was measured to evaluate\nmorphological differences among treatments. The elliptical area was estimated\nfrom measures of the major and minor axes (+ 1 um using a dissecting\nmicroscope (Brazee & Carrington 2006). Proximal breaking stress (N mm-2), a\nmaterial property, was calculated as proximal breaking force divided by\nproximal area. Thread surface structure was examined using a scanning electron\nmicroscope (FEI Sirion XL30 SEM, Hillsboro, OR).\n \nWhole mussel attachment strength was estimated using two mathematical models\ndeveloped by Bell & Gosline (1996). Each model assumes a mussel is anchored\nwith a constant thread number (n=50) arranged in a circle. The normal model\nestimates dislodgment force perpendicular to the substrate (e.g, lift); all\nthreads are engaged and extend until they reach their maximum force. The\nparallel model estimates dislodgement force for an animal pulled parallel to\nthe substrate (e.g., drag); threads on the upstream side are the first in\ntension, yield and extend until they reach maximum force and break, while more\nthreads are recruited into tension until they have all broken. Additionally,\nwe modified each model to incorporate the variation in thread production\nacross treatments. Because thread production was measured for only three days,\ntreatment means were scaled to a maximum value of 50 threads.\n \nDetailed methods and results are provided in Newcomb, 2015 and Newcomb et al.,\n2019\n \nLocation: Friday Harbor Laboratories, Friday Harbor WA
attribute NC_GLOBAL awards_0_award_nid String 55120
attribute NC_GLOBAL awards_0_award_number String OCE-1041213
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward?AWD_ID=1041213 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Mary Beth Saffo
attribute NC_GLOBAL awards_0_program_manager_nid String 51608
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Thread number \n  PI: Emily Carrington \n  Data Version 1: 2019-07-24
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3  19 Dec 2019
attribute NC_GLOBAL date_created String 2019-07-24T20:19:36Z
attribute NC_GLOBAL date_modified String 2019-08-06T19:07:07Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.773623.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/773623 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Materials Testing System
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Instron’s (Norwood, MA) electromechanical testing systems are used to test a wide range of materials in tension or compression. The series 5560 are dual column table top models, the 5565 model has a load capacity of 5 kN (1125 lbf).
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 773637
attribute NC_GLOBAL instruments_0_description String Testing systems that are used to test a wide range of\nmaterials in tension or compression.
attribute NC_GLOBAL instruments_0_instrument_name String Materials Testing System
attribute NC_GLOBAL instruments_0_instrument_nid String 718
attribute NC_GLOBAL instruments_0_supplied_name String Instron 5565 load frame (Norwood, MA)
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, carbon, carbon dioxide, chemical, chemistry, co2, count, data, dataset, dioxide, dmo, earth, Earth Science > Oceans > Ocean Chemistry > pH, erddap, management, measured, mussel, Mussel_ID, ocean, oceanography, oceans, office, pCO2_Measured, pCO2_Target, preliminary, reported, scale, science, sea, sea_water_ph_reported_on_total_scale, seawater, target, Temp, temperature, thread, Thread_Count, total, treatment, Treatment_ID, trial, water
attribute NC_GLOBAL keywords_vocabulary String GCMD Science Keywords
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/773623/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/773623 (external link)
attribute NC_GLOBAL param_mapping String {'773623': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/773623/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Washington
attribute NC_GLOBAL people_0_affiliation_acronym String UW
attribute NC_GLOBAL people_0_person_name String Emily Carrington
attribute NC_GLOBAL people_0_person_nid String 51609
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_1_person_name String Karen Soenen
attribute NC_GLOBAL people_1_person_nid String 748773
attribute NC_GLOBAL people_1_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL project String OA - Ecomaterials Perspective
attribute NC_GLOBAL projects_0_acronym String OA - Ecomaterials Perspective
attribute NC_GLOBAL projects_0_description String Effects of Ocean Acidification on Coastal Organisms: An Ecomaterials Perspective\nThis award will support researchers based at the University of Washington's Friday Harbor Laboratories. The overall focus of the project is to determine how ocean acidification affects the integrity of biomaterials and how these effects in turn alter interactions among members of marine communities. The research plan emphasizes an ecomaterial approach; a team of biomaterials and ecomechanics experts will apply their unique perspective to detail how different combinations of environmental conditions affect the structural integrity and ecological performance of organisms. The study targets a diversity of ecologically important taxa, including bivalves, snails, crustaceans, and seaweeds, thereby providing insight into the range of possible biological responses to future changes in climate conditions. The proposal will enhance our understanding of the ecological consequences of climate change, a significant societal problem.\nEach of the study systems has broader impacts in fields beyond ecomechanics. Engineers are particularly interested in biomaterials and in each system there are materials with commercial potential. The project will integrate research and education by supporting doctoral student dissertation research, providing undergraduate research opportunities via three training programs at FHL, and summer internships for talented high school students, recruited from the FHL Science Outreach Program. The participation of underrepresented groups will be broadened by actively recruiting URM and female students. Results will be disseminated in a variety of forums, including peer-reviewed scientific publications, undergraduate and graduate course material, service learning activities in K-8 classrooms, demonstrations at FHL's annual Open House, and columns for a popular science magazine.
attribute NC_GLOBAL projects_0_end_date String 2013-08
attribute NC_GLOBAL projects_0_geolocation String Friday Harbor, WA
attribute NC_GLOBAL projects_0_name String Effects of Ocean Acidification on Coastal Organisms: An Ecomaterials Perspective
attribute NC_GLOBAL projects_0_project_nid String 2250
attribute NC_GLOBAL projects_0_project_website String http://depts.washington.edu/fhl/oael.html (external link)
attribute NC_GLOBAL projects_0_start_date String 2010-09
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String These data were used in a structural analysis study to evaluate how pCO2 and an additional stressor, elevated temperature, influences byssal thread quality and production. Mussels (M. trossulus) were collected in May 2012 from Argyle Creek, San Juan Island, WA (48.52\\u02da N, 123.01\\u02da W) and held in a mesh box submerged under the dock at Friday Harbor Laboratories (FHL), San Juan Island, WA for up to 14 d. Mussels were placed in controlled temperature and pCO2 treatments in the Ocean Acidification Experimental Laboratory (OAEL), then newly produced threads were counted and pulled to failure to determine byssus strength.
attribute NC_GLOBAL title String [Thread number] - Lab study on the effect of temperature and pCO2 on mussel byssal attachment (thread number) with mussels collected in May 2012 from Argyle Creek, San Juan Island, WA (48.52˚ N, 123.01˚ W) (Effects of Ocean Acidification on Coastal Organisms: An Ecomaterials Perspective)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable Mussel_ID String
attribute Mussel_ID bcodmo_name String sample
attribute Mussel_ID description String Mussel sample identifier
attribute Mussel_ID long_name String Mussel ID
attribute Mussel_ID nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute Mussel_ID units String unitless
variable Temp byte
attribute Temp _FillValue byte 127
attribute Temp actual_range byte 10, 25
attribute Temp bcodmo_name String temperature
attribute Temp description String Temperature
attribute Temp long_name String Temperature
attribute Temp units String degrees Celcius (°C)
variable pH float
attribute pH _FillValue float NaN
attribute pH actual_range float 7.3, 8.2
attribute pH bcodmo_name String pH
attribute pH colorBarMaximum double 9.0
attribute pH colorBarMinimum double 7.0
attribute pH description String pH (total scale)
attribute pH long_name String Sea Water Ph Reported On Total Scale
attribute pH nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/ (external link)
attribute pH units String unitless
variable pCO2_Target short
attribute pCO2_Target _FillValue short 32767
attribute pCO2_Target actual_range short 400, 2500
attribute pCO2_Target bcodmo_name String pCO2
attribute pCO2_Target description String Target pCO2 level
attribute pCO2_Target long_name String P CO2 Target
attribute pCO2_Target nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PCO2C101/ (external link)
attribute pCO2_Target units String micro atmosphere (uatm)
variable pCO2_Measured float
attribute pCO2_Measured _FillValue float NaN
attribute pCO2_Measured actual_range float 226.915, 2838.694
attribute pCO2_Measured bcodmo_name String pCO2
attribute pCO2_Measured description String measured pCO2 level
attribute pCO2_Measured long_name String P CO2 Measured
attribute pCO2_Measured nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PCO2C101/ (external link)
attribute pCO2_Measured units String micro atmosphere (uatm)
variable Treatment_ID String
attribute Treatment_ID bcodmo_name String treatment
attribute Treatment_ID description String Treatment identifier
attribute Treatment_ID long_name String Treatment ID
attribute Treatment_ID units String unitless
variable Thread_Count byte
attribute Thread_Count _FillValue byte 127
attribute Thread_Count actual_range byte 0, 45
attribute Thread_Count bcodmo_name String unknown
attribute Thread_Count colorBarMaximum double 100.0
attribute Thread_Count colorBarMinimum double 0.0
attribute Thread_Count description String Number of byssal threads
attribute Thread_Count long_name String Thread Count
attribute Thread_Count units String unitless
variable Trial byte
attribute Trial _FillValue byte 127
attribute Trial actual_range byte 1, 3
attribute Trial bcodmo_name String exp_id
attribute Trial description String Trial identifier
attribute Trial long_name String Trial
attribute Trial units String unitless

 
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