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Row Type | Variable Name | Attribute Name | Data Type | Value |
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attribute | NC_GLOBAL | access_formats | String | .htmlTable,.csv,.json,.mat,.nc,.tsv |
attribute | NC_GLOBAL | acquisition_description | String | Mussels (M. trossulus) were collected in May 2012 from Argyle Creek, San Juan\nIsland, WA (48.52\\u02da N, 123.01\\u02da W) and held in a mesh box submerged\nunder the dock at Friday Harbor Laboratories (FHL), San Juan Island, WA for up\nto 14 d. Mussels were placed in experimental mesocosms in the Ocean\nAcidification Experimental Laboratory (OAEL) at FHL as described in\nO\\u2019Donnell et al. (2013) and Timmins-Schiffman et al. (2012). Briefly,\nmanipulations of pH were made by bubbling CO2 into a 150 L temperature-\ncontrolled seawater reservoir, that supplied water to eight 3.5 L chambers at\na turnover rate of 50 ml min-1. Air was bubbled into the reservoir to maintain\n100% oxygen saturation and submersible pumps (model number P396, Annex Depot,\nSacramento, CA) provided mixing in the chambers at 3.8 L min-1. The bottom of\neach chamber was lined with autoclaved pebbles, collected from an FHL beach,\nto provide a substrate for byssal thread attachment. pH and temperature were\nmonitored continuously in each water reservoir with a Durafet pH and\ntemperature probe and the full carbonate chemistry of the system evaluated\nwith DIC and Total alkalinity measurements once during each trial. Mussels\nwere acclimated to their treatment temperatures in ambient pH (~7.8) over 9 d,\nramping temperature up no more than 2\\u02daC per day, and fed a maintenance\nlevel of Shellfish Diet 1800 (6 g l-1 day-1, Reed Mariculture, Campell, CA,\nUSA).\n \nThe twelve independent temperature x pCO2 treatments spanned the range of\nlocal marine conditions (Newcomb, 2015; George et al., 2019; temperature at\n10\\u02daC, 18\\u02daC, or 25\\u02daC and pCO2 at 400, 750, 1200, or 2500\n\\u00b5atm). Each mussel was trimmed of external byssus before placement in an\nexperimental treatment for 3 d, sufficient time to produce new mature byssal\nthreads (Bell & Gosline 1996) while minimizing the effect of treatment on\nmussel condition. Mussels were starved during the 3 d trials to minimize\nchanges in chamber water chemistry due to food addition and to reduce fouling.\nThree trials were conducted in succession to replicate treatments over time,\nincreasing sample size (n=8 x 3) for each temperature*pCO2 treatment.\n \nAt the end of each trial, mussels and the rocks to which they had attached\nwith byssal threads were removed from the chambers. The entire byssus was\ndissected from each mussel and stored air-dried for up to 20 days. Byssus was\nrehydrated in seawater prior to testing, a method that does not alter the\nmechanical properties of the byssal threads (Brazee, 2004). The number of\nbyssal threads each mussel produced was counted, and one thread was\nhaphazardly chosen for mechanical testing following the procedure of Bell &\nGosline (1996). Briefly, an individual thread was clamped with submersible\npneumatic grips on either end by holding the proximal byssal stem between\ncardstock with cyanoacrylate glue and affixing the distal plaque with attached\nrock to an aluminum T-bar with epoxy. An Instron 5565 tensometer (Norwood MA,\nUSA), extended the thread at a rate of 10 mm min-1 in a temperature-controlled\nwater bath (3130-100 BioPuls Bath, Instron, Norwood, MA, USA) until failure.\nThe tensometer measured force (\\u00b110-3 N) and extension (\\u00b1 10-3 mm) at\n10 Hz. Tests were performed in seawater with a pH of 7.8 and the relevant\ntreatment temperature.\n \nPull to failure mechanical tests provided estimates of thread breaking force,\nyield force, extensibility, initial stiffness and failure location (Bell &\nGosline 1996). Yield, due to quasi-plastic deformation in the distal region,\nwas identified as the point where the initial slope of the force-extension\ncurve decreased by 40%. Extensibility was calculated by dividing thread\nextension at failure by initial length and initial stiffness was determined\nfrom the initial slope of the force extension curve. The location of failure\n(proximal, plaque, and/or distal region) was noted and threads were retested\nto quantify the breaking force of each remaining region. Tests that broke at\nthe grips were considered underestimates and were discarded.\n \nThe cross-sectional area of the proximal region was measured to evaluate\nmorphological differences among treatments. The elliptical area was estimated\nfrom measures of the major and minor axes (+ 1 um using a dissecting\nmicroscope (Brazee & Carrington 2006). Proximal breaking stress (N mm-2), a\nmaterial property, was calculated as proximal breaking force divided by\nproximal area. Thread surface structure was examined using a scanning electron\nmicroscope (FEI Sirion XL30 SEM, Hillsboro, OR).\n \nWhole mussel attachment strength was estimated using two mathematical models\ndeveloped by Bell & Gosline (1996). Each model assumes a mussel is anchored\nwith a constant thread number (n=50) arranged in a circle. The normal model\nestimates dislodgment force perpendicular to the substrate (e.g, lift); all\nthreads are engaged and extend until they reach their maximum force. The\nparallel model estimates dislodgement force for an animal pulled parallel to\nthe substrate (e.g., drag); threads on the upstream side are the first in\ntension, yield and extend until they reach maximum force and break, while more\nthreads are recruited into tension until they have all broken. Additionally,\nwe modified each model to incorporate the variation in thread production\nacross treatments. Because thread production was measured for only three days,\ntreatment means were scaled to a maximum value of 50 threads.\n \nDetailed methods and results are provided in Newcomb, 2015 and Newcomb et al.,\n2019\n \nLocation: Friday Harbor Laboratories, Friday Harbor WA |
attribute | NC_GLOBAL | awards_0_award_nid | String | 55120 |
attribute | NC_GLOBAL | awards_0_award_number | String | OCE-1041213 |
attribute | NC_GLOBAL | awards_0_data_url | String | http://www.nsf.gov/awardsearch/showAward?AWD_ID=1041213 |
attribute | NC_GLOBAL | awards_0_funder_name | String | NSF Division of Ocean Sciences |
attribute | NC_GLOBAL | awards_0_funding_acronym | String | NSF OCE |
attribute | NC_GLOBAL | awards_0_funding_source_nid | String | 355 |
attribute | NC_GLOBAL | awards_0_program_manager | String | Mary Beth Saffo |
attribute | NC_GLOBAL | awards_0_program_manager_nid | String | 51608 |
attribute | NC_GLOBAL | cdm_data_type | String | Other |
attribute | NC_GLOBAL | comment | String | Thread number \n PI: Emily Carrington \n Data Version 1: 2019-07-24 |
attribute | NC_GLOBAL | Conventions | String | COARDS, CF-1.6, ACDD-1.3 |
attribute | NC_GLOBAL | creator_email | String | info at bco-dmo.org |
attribute | NC_GLOBAL | creator_name | String | BCO-DMO |
attribute | NC_GLOBAL | creator_type | String | institution |
attribute | NC_GLOBAL | creator_url | String | https://www.bco-dmo.org/ |
attribute | NC_GLOBAL | data_source | String | extract_data_as_tsv version 2.3 19 Dec 2019 |
attribute | NC_GLOBAL | date_created | String | 2019-07-24T20:19:36Z |
attribute | NC_GLOBAL | date_modified | String | 2019-08-06T19:07:07Z |
attribute | NC_GLOBAL | defaultDataQuery | String | &time<now |
attribute | NC_GLOBAL | doi | String | 10.1575/1912/bco-dmo.773623.1 |
attribute | NC_GLOBAL | infoUrl | String | https://www.bco-dmo.org/dataset/773623 |
attribute | NC_GLOBAL | institution | String | BCO-DMO |
attribute | NC_GLOBAL | instruments_0_acronym | String | Materials Testing System |
attribute | NC_GLOBAL | instruments_0_dataset_instrument_description | String | Instron’s (Norwood, MA) electromechanical testing systems are used to test a wide range of materials in tension or compression. The series 5560 are dual column table top models, the 5565 model has a load capacity of 5 kN (1125 lbf). |
attribute | NC_GLOBAL | instruments_0_dataset_instrument_nid | String | 773637 |
attribute | NC_GLOBAL | instruments_0_description | String | Testing systems that are used to test a wide range of\nmaterials in tension or compression. |
attribute | NC_GLOBAL | instruments_0_instrument_name | String | Materials Testing System |
attribute | NC_GLOBAL | instruments_0_instrument_nid | String | 718 |
attribute | NC_GLOBAL | instruments_0_supplied_name | String | Instron 5565 load frame (Norwood, MA) |
attribute | NC_GLOBAL | keywords | String | bco, bco-dmo, biological, carbon, carbon dioxide, chemical, chemistry, co2, count, data, dataset, dioxide, dmo, earth, Earth Science > Oceans > Ocean Chemistry > pH, erddap, management, measured, mussel, Mussel_ID, ocean, oceanography, oceans, office, pCO2_Measured, pCO2_Target, preliminary, reported, scale, science, sea, sea_water_ph_reported_on_total_scale, seawater, target, Temp, temperature, thread, Thread_Count, total, treatment, Treatment_ID, trial, water |
attribute | NC_GLOBAL | keywords_vocabulary | String | GCMD Science Keywords |
attribute | NC_GLOBAL | license | String | https://www.bco-dmo.org/dataset/773623/license |
attribute | NC_GLOBAL | metadata_source | String | https://www.bco-dmo.org/api/dataset/773623 |
attribute | NC_GLOBAL | param_mapping | String | {'773623': {}} |
attribute | NC_GLOBAL | parameter_source | String | https://www.bco-dmo.org/mapserver/dataset/773623/parameters |
attribute | NC_GLOBAL | people_0_affiliation | String | University of Washington |
attribute | NC_GLOBAL | people_0_affiliation_acronym | String | UW |
attribute | NC_GLOBAL | people_0_person_name | String | Emily Carrington |
attribute | NC_GLOBAL | people_0_person_nid | String | 51609 |
attribute | NC_GLOBAL | people_0_role | String | Principal Investigator |
attribute | NC_GLOBAL | people_0_role_type | String | originator |
attribute | NC_GLOBAL | people_1_affiliation | String | Woods Hole Oceanographic Institution |
attribute | NC_GLOBAL | people_1_affiliation_acronym | String | WHOI BCO-DMO |
attribute | NC_GLOBAL | people_1_person_name | String | Karen Soenen |
attribute | NC_GLOBAL | people_1_person_nid | String | 748773 |
attribute | NC_GLOBAL | people_1_role | String | BCO-DMO Data Manager |
attribute | NC_GLOBAL | people_1_role_type | String | related |
attribute | NC_GLOBAL | project | String | OA - Ecomaterials Perspective |
attribute | NC_GLOBAL | projects_0_acronym | String | OA - Ecomaterials Perspective |
attribute | NC_GLOBAL | projects_0_description | String | Effects of Ocean Acidification on Coastal Organisms: An Ecomaterials Perspective\nThis award will support researchers based at the University of Washington's Friday Harbor Laboratories. The overall focus of the project is to determine how ocean acidification affects the integrity of biomaterials and how these effects in turn alter interactions among members of marine communities. The research plan emphasizes an ecomaterial approach; a team of biomaterials and ecomechanics experts will apply their unique perspective to detail how different combinations of environmental conditions affect the structural integrity and ecological performance of organisms. The study targets a diversity of ecologically important taxa, including bivalves, snails, crustaceans, and seaweeds, thereby providing insight into the range of possible biological responses to future changes in climate conditions. The proposal will enhance our understanding of the ecological consequences of climate change, a significant societal problem.\nEach of the study systems has broader impacts in fields beyond ecomechanics. Engineers are particularly interested in biomaterials and in each system there are materials with commercial potential. The project will integrate research and education by supporting doctoral student dissertation research, providing undergraduate research opportunities via three training programs at FHL, and summer internships for talented high school students, recruited from the FHL Science Outreach Program. The participation of underrepresented groups will be broadened by actively recruiting URM and female students. Results will be disseminated in a variety of forums, including peer-reviewed scientific publications, undergraduate and graduate course material, service learning activities in K-8 classrooms, demonstrations at FHL's annual Open House, and columns for a popular science magazine. |
attribute | NC_GLOBAL | projects_0_end_date | String | 2013-08 |
attribute | NC_GLOBAL | projects_0_geolocation | String | Friday Harbor, WA |
attribute | NC_GLOBAL | projects_0_name | String | Effects of Ocean Acidification on Coastal Organisms: An Ecomaterials Perspective |
attribute | NC_GLOBAL | projects_0_project_nid | String | 2250 |
attribute | NC_GLOBAL | projects_0_project_website | String | http://depts.washington.edu/fhl/oael.html |
attribute | NC_GLOBAL | projects_0_start_date | String | 2010-09 |
attribute | NC_GLOBAL | publisher_name | String | Biological and Chemical Oceanographic Data Management Office (BCO-DMO) |
attribute | NC_GLOBAL | publisher_type | String | institution |
attribute | NC_GLOBAL | sourceUrl | String | (local files) |
attribute | NC_GLOBAL | standard_name_vocabulary | String | CF Standard Name Table v55 |
attribute | NC_GLOBAL | summary | String | These data were used in a structural analysis study to evaluate how pCO2 and an additional stressor, elevated temperature, influences byssal thread quality and production. Mussels (M. trossulus) were collected in May 2012 from Argyle Creek, San Juan Island, WA (48.52\\u02da N, 123.01\\u02da W) and held in a mesh box submerged under the dock at Friday Harbor Laboratories (FHL), San Juan Island, WA for up to 14 d. Mussels were placed in controlled temperature and pCO2 treatments in the Ocean Acidification Experimental Laboratory (OAEL), then newly produced threads were counted and pulled to failure to determine byssus strength. |
attribute | NC_GLOBAL | title | String | [Thread number] - Lab study on the effect of temperature and pCO2 on mussel byssal attachment (thread number) with mussels collected in May 2012 from Argyle Creek, San Juan Island, WA (48.52˚ N, 123.01˚ W) (Effects of Ocean Acidification on Coastal Organisms: An Ecomaterials Perspective) |
attribute | NC_GLOBAL | version | String | 1 |
attribute | NC_GLOBAL | xml_source | String | osprey2erddap.update_xml() v1.3 |
variable | Mussel_ID | String | ||
attribute | Mussel_ID | bcodmo_name | String | sample |
attribute | Mussel_ID | description | String | Mussel sample identifier |
attribute | Mussel_ID | long_name | String | Mussel ID |
attribute | Mussel_ID | nerc_identifier | String | https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ |
attribute | Mussel_ID | units | String | unitless |
variable | Temp | byte | ||
attribute | Temp | _FillValue | byte | 127 |
attribute | Temp | actual_range | byte | 10, 25 |
attribute | Temp | bcodmo_name | String | temperature |
attribute | Temp | description | String | Temperature |
attribute | Temp | long_name | String | Temperature |
attribute | Temp | units | String | degrees Celcius (°C) |
variable | pH | float | ||
attribute | pH | _FillValue | float | NaN |
attribute | pH | actual_range | float | 7.3, 8.2 |
attribute | pH | bcodmo_name | String | pH |
attribute | pH | colorBarMaximum | double | 9.0 |
attribute | pH | colorBarMinimum | double | 7.0 |
attribute | pH | description | String | pH (total scale) |
attribute | pH | long_name | String | Sea Water Ph Reported On Total Scale |
attribute | pH | nerc_identifier | String | https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/ |
attribute | pH | units | String | unitless |
variable | pCO2_Target | short | ||
attribute | pCO2_Target | _FillValue | short | 32767 |
attribute | pCO2_Target | actual_range | short | 400, 2500 |
attribute | pCO2_Target | bcodmo_name | String | pCO2 |
attribute | pCO2_Target | description | String | Target pCO2 level |
attribute | pCO2_Target | long_name | String | P CO2 Target |
attribute | pCO2_Target | nerc_identifier | String | https://vocab.nerc.ac.uk/collection/P01/current/PCO2C101/ |
attribute | pCO2_Target | units | String | micro atmosphere (uatm) |
variable | pCO2_Measured | float | ||
attribute | pCO2_Measured | _FillValue | float | NaN |
attribute | pCO2_Measured | actual_range | float | 226.915, 2838.694 |
attribute | pCO2_Measured | bcodmo_name | String | pCO2 |
attribute | pCO2_Measured | description | String | measured pCO2 level |
attribute | pCO2_Measured | long_name | String | P CO2 Measured |
attribute | pCO2_Measured | nerc_identifier | String | https://vocab.nerc.ac.uk/collection/P01/current/PCO2C101/ |
attribute | pCO2_Measured | units | String | micro atmosphere (uatm) |
variable | Treatment_ID | String | ||
attribute | Treatment_ID | bcodmo_name | String | treatment |
attribute | Treatment_ID | description | String | Treatment identifier |
attribute | Treatment_ID | long_name | String | Treatment ID |
attribute | Treatment_ID | units | String | unitless |
variable | Thread_Count | byte | ||
attribute | Thread_Count | _FillValue | byte | 127 |
attribute | Thread_Count | actual_range | byte | 0, 45 |
attribute | Thread_Count | bcodmo_name | String | unknown |
attribute | Thread_Count | colorBarMaximum | double | 100.0 |
attribute | Thread_Count | colorBarMinimum | double | 0.0 |
attribute | Thread_Count | description | String | Number of byssal threads |
attribute | Thread_Count | long_name | String | Thread Count |
attribute | Thread_Count | units | String | unitless |
variable | Trial | byte | ||
attribute | Trial | _FillValue | byte | 127 |
attribute | Trial | actual_range | byte | 1, 3 |
attribute | Trial | bcodmo_name | String | exp_id |
attribute | Trial | description | String | Trial identifier |
attribute | Trial | long_name | String | Trial |
attribute | Trial | units | String | unitless |