BCO-DMO ERDDAP
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Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Experiments were conducted by collecting raw seawater, filtering it through\n200\\u00b5m mesh sieves into 20L carboys, and then dispensing it into\nexperimental jars. Triplicates bottles were used for each treatment.\nTreatments included whole seawater (control), whole seawater plus copepods\n(Zooplankton), and the same treatments plus PUA additions (Heptadienal,\nOctadienal, Decadienal, and Mixed PUA). PUA were dissolved in methanol and\nadded to experimental bottles for a final concentration of 21 nM; for the\nmixed PUA treatment this was 7nM of each type of PUA.\n \nInitial samples were collected from the carboy for microzooplankton as\ndescribed below. Final samples were collected from each treatment and control\nbottle as described below.\n \nMicrozooplankton samples were collected by gently decanting 100ml of each\ntreatment bottle into a sample bottle and preserved with 2% acid Lugol\\u2019s\nsolution (final concentration). Identification and sorting of microzooplankton\nwas done by settling 10-25 ml of sample in Uterm\\u00f6hl chambers and counting\nwith an Olympus IX-70 inverted microscope equipped with differential\ninterference contrast (DIC), epifluorescence, and a digital camera.\nMicrozooplankton biovolumes were calculated from their dimensions and\napproximate shapes (Sun and Liu 2003), and converted to carbon using published\nempirical relationships (Menden-Deuer and Lessard 2000). Tintinnid volumes\nwere calculated based on their cell dimensions.\n \nAll data were processed in Microsoft Excel.
attribute NC_GLOBAL awards_0_award_nid String 555555
attribute NC_GLOBAL awards_0_award_number String OCE-1357168
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1357168 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_0_program_manager_nid String 50446
attribute NC_GLOBAL awards_1_award_nid String 555561
attribute NC_GLOBAL awards_1_award_number String OCE-1357169
attribute NC_GLOBAL awards_1_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1357169 (external link)
attribute NC_GLOBAL awards_1_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_1_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_1_funding_source_nid String 355
attribute NC_GLOBAL awards_1_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_1_program_manager_nid String 50446
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String PUA (polyunsaturated aldehydes) experiments: Microzooplankton Biomass \n   Virginia Coastal Bays and Bay of Napoli, Mar-July 2015 \n   PI's: P. Lavrentyev (U Akron), J. Pierson & D. Stoeker (UMaryland CES) \n   version date: 2019-09-30
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3  19 Dec 2019
attribute NC_GLOBAL date_created String 2019-08-01T15:21:48Z
attribute NC_GLOBAL date_modified String 2019-10-07T20:53:53Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.774033.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/774033 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Inverted Microscope
attribute NC_GLOBAL instruments_0_dataset_instrument_description String The microscope was equipped with differential interference contrast (DIC), epifluorescence, and a digital camera. Biomass estimates for microzooplankton were determined after counting cells on an inverted microscope and converting volume estimates, from measurements with a reticle in the objective lens, to carbon concentrations using known conversion factors.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 774048
attribute NC_GLOBAL instruments_0_description String An inverted microscope is a microscope with its light source and condenser on the top, above the stage pointing down, while the objectives and turret are below the stage pointing up. It was invented in 1850 by J. Lawrence Smith, a faculty member of Tulane University (then named the Medical College of Louisiana).\n\nInverted microscopes are useful for observing living cells or organisms at the bottom of a large container (e.g. a tissue culture flask) under more natural conditions than on a glass slide, as is the case with a conventional microscope. Inverted microscopes are also used in micromanipulation applications where space above the specimen is required for manipulator mechanisms and the microtools they hold, and in metallurgical applications where polished samples can be placed on top of the stage and viewed from underneath using reflecting objectives.\n\nThe stage on an inverted microscope is usually fixed, and focus is adjusted by moving the objective lens along a vertical axis to bring it closer to or further from the specimen. The focus mechanism typically has a dual concentric knob for coarse and fine adjustment. Depending on the size of the microscope, four to six objective lenses of different magnifications may be fitted to a rotating turret known as a nosepiece. These microscopes may also be fitted with accessories for fitting still and video cameras, fluorescence illumination, confocal scanning and many other applications.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB05/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Inverted Microscope
attribute NC_GLOBAL instruments_0_instrument_nid String 675
attribute NC_GLOBAL instruments_0_supplied_name String Olympus IX-70 inverted microscope
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, biomass, bottle, Bottle_ID, chemical, data, dataset, dmo, erddap, esd, experiment, management, oceanography, office, phylum, preliminary, rep, taxa, treatment
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/774033/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/774033 (external link)
attribute NC_GLOBAL param_mapping String {'774033': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/774033/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Akron
attribute NC_GLOBAL people_0_affiliation_acronym String UAkron
attribute NC_GLOBAL people_0_person_name String Dr Peter Lavrentyev
attribute NC_GLOBAL people_0_person_nid String 555559
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String University of Maryland Center for Environmental Science
attribute NC_GLOBAL people_1_affiliation_acronym String UMCES/HPL
attribute NC_GLOBAL people_1_person_name String James J. Pierson
attribute NC_GLOBAL people_1_person_nid String 51109
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String University of Maryland Center for Environmental Science
attribute NC_GLOBAL people_2_affiliation_acronym String UMCES/HPL
attribute NC_GLOBAL people_2_person_name String Diane Stoecker
attribute NC_GLOBAL people_2_person_nid String 50623
attribute NC_GLOBAL people_2_role String Co-Principal Investigator
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_3_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_3_person_name String Nancy Copley
attribute NC_GLOBAL people_3_person_nid String 50396
attribute NC_GLOBAL people_3_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL project String DiatomAldehydes
attribute NC_GLOBAL projects_0_acronym String DiatomAldehydes
attribute NC_GLOBAL projects_0_description String Description from NSF award abstract:\nThis project will conduct a set of field/laboratory experiments to address the following hypotheses with respect to microzooplankton (consumers between 20-200 um) and diatom- produced polyunsaturated aldehydes:\nI. Aldehydes will impair microzooplankton herbivory on diatoms and non-diatom phytoplankton.\nII. Aldehydes will reduce the growth rates of microzooplankton and non PUA-producing phytoplankton.\nIII. In the presence of aldehyde-producing diatoms, copepods will switch to microzooplankton, whereas non- (mildly)- toxic diatoms will be an important food source for copepods.\nIV. The effects of aldehydes on microzooplankton and copepods will depend on the grazers' prior exposure to PUA.\nThe experiments will include natural plankton, captured copepods, cultured Skeletonema marinoi (SM), including its aldehyde-producing strain, and synthetic aldehydes. To gain insights into complex interactions within planktonic communities, detailed information on their composition, abundance, and dynamics will be obtained using microscopy, flow-cytometry, and cytological methods. This approach will allow the PIs to draw conclusions about the role of diatom-produced aldehydes in phytoplankton-microzooplankton- copepod trophic interactions. The PIs will coordinate efforts and exchange information with the PUA study group at the Stazione Zoologica Anton Dohrn (Naples, Italy).\nDiatoms are dominant autotrophic plankton in the ocean. Recent evidence indicates that microzooplankton are the dominant herbivores, whereas copepods often rely on microzooplankton as food, except during peak diatom production. The ability of microzooplankton to feed on large diatoms and grow as fast as their algal prey leads to the question of what allows diatoms to escape microzooplankton grazing control during the initial phases of their blooms and maintain the blooms until nutrient resources are depleted? Allelopathy is wide spread among phytoplankton. The cosmopolitan bloom-forming SM produces several aldehydes and has become a model organism in plankton allelopathy studies. Most studies on diatom cytotoxicity have been dedicated to inhibitory effects on reproduction and development of marine invertebrates, whereas surprisingly little information exists on its impact on key diatom grazers, microzooplankton. Preliminary results in the Chesapeake Bay show that aldehydes may induce cascading effects within planktonic communities. The proposed study will: (1) Improve our knowledge of the critical diatom-microzooplankton-copepod links in the coastal ocean; (2) Generate novel data on the effects of allelopathy on marine food webs; (3) Contribute to our understanding of broader patterns of marine ecosystems by comparing plankton structure and dynamics in the temperate Atlantic waters; (4) Advance biological oceanography through international collaboration.
attribute NC_GLOBAL projects_0_end_date String 2017-03
attribute NC_GLOBAL projects_0_name String The effects of diatom-produced polyunsaturated aldehydes on the microbial food web in temperate and polar waters
attribute NC_GLOBAL projects_0_project_nid String 555556
attribute NC_GLOBAL projects_0_start_date String 2014-04
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Microzooplankton biomass estimates from PUA (polyunsaturated aldehydes) experiments, Virginia Coastal Bays and Bay of Napoli, Mar-July 2015.
attribute NC_GLOBAL title String [PUA Microzooplankton Biomass] - Microzooplankton biomass estimates from PUA (polyunsaturated aldehydes) experiments, Virginia Coastal Bays and Bay of Napoli, Mar-July 2015 (The effects of diatom-produced polyunsaturated aldehydes on the microbial food web in temperate and polar waters)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable Experiment String
attribute Experiment bcodmo_name String exp_id
attribute Experiment description String Name of the Experiment
attribute Experiment long_name String Experiment
attribute Experiment units String unitless
variable Bottle_ID String
attribute Bottle_ID bcodmo_name String sample
attribute Bottle_ID description String ID name for a given treatment including both treatment and replicate tags
attribute Bottle_ID long_name String Bottle ID
attribute Bottle_ID nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute Bottle_ID units String unitless
variable Treatment String
attribute Treatment bcodmo_name String treatment
attribute Treatment description String Treatment name
attribute Treatment long_name String Treatment
attribute Treatment units String unitless
variable Rep byte
attribute Rep _FillValue byte 127
attribute Rep actual_range byte 1, 3
attribute Rep bcodmo_name String replicate
attribute Rep description String Replicate
attribute Rep long_name String Rep
attribute Rep units String unitless
variable Taxa String
attribute Taxa bcodmo_name String taxon
attribute Taxa description String Taxonomic name of microzooplankton type identified
attribute Taxa long_name String Taxa
attribute Taxa units String unitless
variable Phylum String
attribute Phylum bcodmo_name String phylum
attribute Phylum description String Phylum name for the taxa identified
attribute Phylum long_name String Phylum
attribute Phylum units String unitless
variable ESD byte
attribute ESD _FillValue byte 127
attribute ESD actual_range byte 9, 58
attribute ESD bcodmo_name String cell_size
attribute ESD description String Mean size of the taxa; given as equivalent spherical diameter (ESD)
attribute ESD long_name String ESD
attribute ESD units String micrometers
variable Biomass float
attribute Biomass _FillValue float NaN
attribute Biomass actual_range float 0.0033, 100.9486
attribute Biomass bcodmo_name String biomass
attribute Biomass description String Biomass of the given taxa
attribute Biomass long_name String Biomass
attribute Biomass units String micrograms carbon/liter

 
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