BCO-DMO ERDDAP
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Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute NC_GLOBAL acquisition_description String Samples for this study come from CORK observatories installed at IODP Holes\nU1382A and U1383C as described elsewhere (Edwards et al., 2012b). In brief,\ninstrument strings containing OsmoSampler systems (Wheat et al., 2011) were\ndeployed at different depths within the holes from 2011-2017. Additional\nOsmoSampler systems were deployed at the wellhead of the CORKs in 2014 and\nwere inoculated with bottom seawater, making them useful for identifying\ndifferences between crustal subsurface and bottom seawater inoculated\nmicrobial communities. OsmoSampler systems included Flow-through Osmo\nColonization Systems (FLOCS) for mineral colonization experiments, as\ndescribed elsewhere (Ramrez et al., 2019). Each FLOCS contained sterile\n(autoclaved and ethanol-rinsed) substrates including crushed basalts, pyrite,\npyrrhotite, or inert glass beads housed within polycarbonate cassettes and\nsleeves. Fluids were introduced into the FLOCS via the OsmoSampler pumps,\nallowing fluid microbial communities to colonize the substrates. All FLOCS\nwere recovered in October 2017 during cruise AT39-01 of the RV Atlantis with\nthe ROV Jason (Woods Hole Oceanographic Institution) following methods\ndescribed elsewhere (Ramrez et al., 2019). In brief, the instrument strings\nwith the downhole FLOCS were pulled up to the ship on a wire, then immediately\ndisassembled to store the FLOCS in a cold room. Wellhead FLOCS incubated in\nmilkcrates attached to crustal fluid umbilicals at the seafloor were also\ncollected on this cruise with the ROV. FLOCS contents were distributed inside\nan ethanol-rinsed and UV-irradiated HEPA-filtered hood using sterile\ninstruments. Substrates for the experiment were stored cold (2-4C) and in the\ndark in sterile centrifuge tubes with ultrafiltered (0.22 m-mesh Millipore\nSterivex and 0.02 m-mesh Whatman Anotop 25 filters) crustal fluid until\nincubation at the shore-based laboratory. Parallel samples for initial\ncharacterization of the substrate biofilms were transferred to sterile\ncyrovials, flash frozen with liquid nitrogen, and stored at -80C until\nanalysis. In addition to the FLOCS samples, 4-10L samples of raw and\nunfiltered crustal fluids were collected into ethanol-rinsed cubitainers after\ncollection into gamma-irradiated bags on the seafloor using a Mobile Pumping\nSystem. Cubitainers were stored cold (2-4C) and in the dark for approximately\none year before beginning the experiments. A detailed description of the\ncathodic poised potential protocol used is available elsewhere (Jones and\nOrcutt, 2019). In brief, glass two-cell, three-electrode MFC systems (Adams\nand Chittenden, CA, USA) were used as incubations chambers, run in parallel\nwith a multichannel potentiostat (model CHI1030C, CHI Instruments, TX, USA).\nNafion 117 proton exchange membrane (Fuel Cell Store, TX) separated the half-\ncells. Cells were filled with distilled water and autoclaved prior to filling\nwith media and sample inocula. Ag/AgCl reference electrodes (Gamry\nInstruments, PA, USA part 932-00018 and/or Analytical Instrument Systems, NY,\nUSA) were calibrated before each run by immersion in 3 M NaCl and compared\nagainst a known electrode kept only for that purpose (max +/- 20 mV drift from\nthe value of lab master electrode). Working electrodes (WEs) were 2 4 cm2\nIndium Tin Oxide (ITO) coated glass slides (Delta Technologies Ltd, CO, USA\npart CB-50IN-1111), constructed for MFC as described elsewhere (Rowe et al.,\n2015). Counter electrodes (CEs) were carbon cloth with a 4 4 cm2 surface area.\nElectrodes were sterilized by rinsing with 80% ethanol, air-drying, then\nexposing to UV radiation for 15 minutes per side. Each experiment consisted of\nfive treatments: Three MFCs filled with buffered and double autoclaved crustal\nfluid (cool and oxic), inoculated with samples, and incubated with a WE set\npoised at -200 mV versus a standard hydrogen electrode (SHE) referred to as\nthe Echem treatments; one MFC filled with the same fluid and WE but without\nany sample inoculum referred to as the Fluid treatment; and one glass bottle\nwith a mixed sample inoculum and ITO electrode without any voltage applied\nreferred to as the Offline treatment. Samples were transferred into the MFCs\nin HEPA-filtered, UV-irradiated biosafety cabinet or hood using sterile tools.\nAn aliquot of each sample was also collected into a sterile plastic tube for\nmicrobial community analysis representing a time zero (T0) condition that may\nhave changed from the Shipboard (SB) initial condition. For the Echem and\nFluid treatments, the counter cell redox couple was H2O/O2. Testing (data not\nshown) determined that a strong kill control method was necessary to achieve\nsterility of the crustal fluid, consisting of 1 h autoclaving at 121C,\nincubation at room temperature in the dark for ~24 h to allow for spore\ngermination, and then another 1 h autoclaving at 121C before cooling down to\n4C for the addition of substrate. Sodium bicarbonate was added to the media\n(0.1 M) as a buffer. MFCs were incubated at 4C in the dark. Once the MFCs were\nconstructed, a cyclic voltammetry (CV) sweep was performed for each cell with\nthe potentiostat before each poised potential experiment at a scan rate of 0.1\nV s-1 and sample interval of 0.001 V, across -1 to 1 V range. Then the\nchronoamperometry (CA) experiments began by applying a constant voltage (-200\nmV versus SHE) to the Echem and Fluid MFCs. Current generation was monitored\nand recorded until current changes began to decline after a period of\napproximately 15 days. At the end of each CA experiment, two further CV sweeps\nwere performed: one with the incubated ITO electrode (Tend i) and one with a\nfresh ITO electrode dipped into the incubated media (Tend ii).
attribute NC_GLOBAL awards_0_award_nid String 707761
attribute NC_GLOBAL awards_0_award_number String OCE-1536539
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1536539 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Barbara Ransom
attribute NC_GLOBAL awards_0_program_manager_nid String 661067
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String CA Experimental metadata \n  PI: Beth Orcutt \n  Data Version 1: 2019-10-30
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3  19 Dec 2019
attribute NC_GLOBAL date_created String 2019-10-30T18:45:33Z
attribute NC_GLOBAL date_modified String 2020-02-11T16:02:21Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.780225.1
attribute NC_GLOBAL Easternmost_Easting double -46.053
attribute NC_GLOBAL geospatial_lat_max double 22.8023
attribute NC_GLOBAL geospatial_lat_min double 22.7564
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -46.053
attribute NC_GLOBAL geospatial_lon_min double -46.0817
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/780225 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Voltammetry Analyzers
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 780231
attribute NC_GLOBAL instruments_0_description String Instruments that obtain information about an analyte by applying a potential and measuring the current produced in the analyte.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB35/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Voltammetry Analyzers
attribute NC_GLOBAL instruments_0_instrument_nid String 698
attribute NC_GLOBAL instruments_0_supplied_name String multichannel potentiostat (model CHI1030C, CHI Instruments, TX, USA)
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, borehole, channel, chemical, cruise, data, dataset, date, depth, dmo, erddap, experiment, ExperimentID, flocsdeploy, flocsdeployment, FLOCSdeploymentCruise, flocsdepth, flocsname, flocsrecover, FLOCSrecoverCruise, FLOCSrecoverDate, flocssource, FLOCSsourceDepth, flocssubstrate, flocstype, fluid, fluidcollection, FluidcollectionCruise, FluidcollectionDate, FluidSource, latitude, longitude, management, mediasource, MediasourceDepth, oceanography, office, preliminary, source, time, treatment, water, WaterDepth
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/780225/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/780225 (external link)
attribute NC_GLOBAL Northernmost_Northing double 22.8023
attribute NC_GLOBAL param_mapping String {'780225': {'Latitude': 'flag - latitude', 'Longitude': 'flag - longitude'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/780225/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Bigelow Laboratory for Ocean Sciences
attribute NC_GLOBAL people_0_person_name String Beth N. Orcutt
attribute NC_GLOBAL people_0_person_nid String 565799
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_1_person_name String Amber York
attribute NC_GLOBAL people_1_person_nid String 643627
attribute NC_GLOBAL people_1_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL project String North Pond 2017
attribute NC_GLOBAL projects_0_acronym String North Pond 2017
attribute NC_GLOBAL projects_0_description String NSF Award Abstract:\nSeawater circulates through the upper part of the oceanic crust much like groundwater flows through continental aquifers. However, in the ocean this seawater circulation, many times heated by buried magmatic bodies, transports and releases 25% of the Earth's heat. The rate of fluid flow through ocean crust is estimated to be equal to the amount of water delivered by rivers to the ocean. Much of what we know of this subseafloor fluid flow comes from studies in the eastern Pacific Ocean on ocean crust created by medium and fast spreading mid-ocean ridges. These studies indicate that seawater and its circulation through the seafloor significantly impact crustal evolution and biogeochemical cycles in the ocean and affect the biosphere in ways that are just now beginning to be quantified and understood. To expand this understanding, this research focuses on fluid flow of seafloor generated by slow spreading ridges, like those in the Atlantic, Indian and Arctic Oceans because it is significantly different in structure, mineralogy, and morphology than that formed at fast and intermediate spreading ridges. This research returns to North Pond, a long-term; seafloor; fluid flow monitoring site, drilled and instumented by the Ocean Drilling Program in the Atlantic Ocean. This research site was punctured by boreholes in which fluid flow and geochemical and biological samplers have been deployed for a number of years to collect data and samples. It also provides resources for shipboard and on-shore geochemical and biological analysis. Broader impacts of the work include sensor and technology development, which increases infrastructure for science and has commercial applications. It also provides training for students and the integration of education and research at three US academic institutions, one of which is an EPSCoR state (Mississippi), and supports a PI whose gender is under-represented in sciences and engineering. Public outreach will be carried out in conjunction with the Center for Dark Energy Biosphere Investigations.\nThis project completes a long-term biogeochemical and hydrologic study of ridge flank hydrothermal processes on slow-spreading, 8 million year old crust on the western flank of the Mid-Atlantic Ridge. The site, North Pond, is an isolated northeast-trending sediment pond, bounded by undersea mountains that have been studied since the 1970s. During Integrated Ocean Drilling Program Expedition 336 in 2011 and an expedition five months later (2012), sensors, samplers, and experiments were deployed in four borehole observatories drilled into the seafloor that penetrated into volcanic crust, with the purpose of monitoring changes in hydrologic properties, crustal fluid composition and mineral alteration, among other objectives. Wellhead sampling in 2012 and 2014 already revealed changes in crustal fluid compositions; and associated pressure data confirm that the boreholes are sealed and overpressured, reflecting a change in the formation as the boreholes recover from drilling disturbances. This research includes a 13-day oceanographic expedition and use of on-site robotically operated vehicles to recover downhole instrument packages at North Pond. It will allow the sampling of crustal fluids, recovering pressure data, and measuring fluid flow rates. Ship- and shore-based analyses will be used to address fundamental questions related to the hydrogeology of hydrothermal processes on slow-spread crust.
attribute NC_GLOBAL projects_0_end_date String 2018-09
attribute NC_GLOBAL projects_0_geolocation String North Pond, Mid-Atlantic Ridge flank CORKs
attribute NC_GLOBAL projects_0_name String Collaborative Research: Completing North Pond Borehole Experiments to Elucidate the Hydrology of Young, Slow-Spread Crust
attribute NC_GLOBAL projects_0_project_nid String 707762
attribute NC_GLOBAL projects_0_project_website String http://www.darkenergybiosphere.org/research-activities/field-sites/ (external link)
attribute NC_GLOBAL projects_0_start_date String 2015-10
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 22.7564
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Metadata for cathodic poised potential experiments with subsurface crustal samples from CORK borehole observatories at North Pond on the Mid-Atlantic Ridge during R/V Atlantis cruise AT39-01.
attribute NC_GLOBAL title String [AT39-01 CathodicEET Experiment Metadata] - Metadata for cathodic poised potential experiments with subsurface crustal samples from CORK borehole observatories at North Pond on the Mid-Atlantic Ridge during R/V Atlantis cruise AT39-01 (Collaborative Research: Completing North Pond Borehole Experiments to Elucidate the Hydrology of Young, Slow-Spread Crust)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -46.0817
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable ExperimentID String
attribute ExperimentID bcodmo_name String exp_id
attribute ExperimentID description String Text description of experiment with microbial fuel cells; values of NP11, NP12, NP13, NP14 or NP15
attribute ExperimentID long_name String Experiment ID
attribute ExperimentID units String unitless
variable Channel String
attribute Channel bcodmo_name String sample_descrip
attribute Channel description String Channel used in the poised potential experiment microbial fuel cell; values of NA (not applicable), CH1 (channel 1), CH2 (channel 2), CH3 (channel 3), or CH4 (channel 4)
attribute Channel long_name String Channel
attribute Channel units String unitless
variable Treatment String
attribute Treatment bcodmo_name String treatment
attribute Treatment description String Treatment of the microbial fuel cell; values of Offline (with sample but no voltage applied), Fluid (with filtered fluid only/no sample and with voltage applied), or Echem (with sample and voltage applied)
attribute Treatment long_name String Treatment
attribute Treatment units String unitless
variable FLOCSsubstrate String
attribute FLOCSsubstrate bcodmo_name String sample_descrip
attribute FLOCSsubstrate description String Description of the FLOCS sample type put into the microbial fuel cell; values of Mixed (a mixture of samples), NA (not applicable, no sample), Basalt, Glass Wool, or Pyrrhotite
attribute FLOCSsubstrate long_name String FLOCSsubstrate
attribute FLOCSsubstrate units String unitless
variable Borehole String
attribute Borehole bcodmo_name String site_descrip
attribute Borehole description String Name of the IODP borehole that was the location of the FLOCS experiment; values of U1382A (from IODP Hole U1382A) or U1383C (from IODP Hole U1383C)
attribute Borehole long_name String Borehole
attribute Borehole units String unitless
variable FluidSource String
attribute FluidSource bcodmo_name String site_descrip
attribute FluidSource description String Name of the IODP borehole location where crustal fluid was collected for preparing microbial fuel cell media; values of U1382A (from IODP Hole U1382A shallow horizon), U1383Cdeep (from IODP Hole U1383C deep horizon), or U1383Cshallow (from IODP Hole U1383C shallow horizon)
attribute FluidSource long_name String Fluid Source
attribute FluidSource units String unitless
variable FLOCStype String
attribute FLOCStype bcodmo_name String exp_type
attribute FLOCStype description String Type of the FLOCS experiment; values of U1382A downhole (recovered from the shallow horizon of U1382A), U1382A wellhead (from the wellhead of IODP Hole U1382A), and U1383C wellhead bottom water (from IODP Hole U1383C wellhead)
attribute FLOCStype long_name String FLOCStype
attribute FLOCStype units String unitless
variable FLOCSname String
attribute FLOCSname bcodmo_name String exp_type
attribute FLOCSname description String Name of the FLOCS experiment
attribute FLOCSname long_name String FLOCSname
attribute FLOCSname units String unitless
variable latitude double
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 22.7564, 22.8023
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String Latitude of the FLOCS experiment
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude double
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -46.0817, -46.053
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String Longitude of the FLOCS experiment
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable WaterDepth short
attribute WaterDepth _FillValue short 32767
attribute WaterDepth actual_range short 4414, 4483
attribute WaterDepth bcodmo_name String depth_w
attribute WaterDepth description String water depth to seafloor of the IODP Hole
attribute WaterDepth long_name String Water Depth
attribute WaterDepth units String meters (m)
variable FLOCSdepth String
attribute FLOCSdepth bcodmo_name String meters below seafloor
attribute FLOCSdepth description String Approximate depth, in meters below seafloor, where the FLOCS experiment was deployed
attribute FLOCSdepth long_name String FLOCSdepth
attribute FLOCSdepth units String meters below seafloor (m)
variable FLOCSsourceDepth String
attribute FLOCSsourceDepth bcodmo_name String meters below seafloor
attribute FLOCSsourceDepth description String Approximate depth, in meters below seafloor, where the crustal fluids that inoculated the FLOCS were sourced from
attribute FLOCSsourceDepth long_name String FLOCSsource Depth
attribute FLOCSsourceDepth units String meters below seafloor (m)
variable MediasourceDepth String
attribute MediasourceDepth bcodmo_name String meters below seafloor
attribute MediasourceDepth description String Approximate depth, in meters below seafloor, where the crustal fluids were collected to prepare media for the microbial fuel cells
attribute MediasourceDepth long_name String Mediasource Depth
attribute MediasourceDepth units String meters below seafloor (m)
variable FLOCSdeploymentCruise String
attribute FLOCSdeploymentCruise bcodmo_name String cruise_id
attribute FLOCSdeploymentCruise description String Name of the cruise (and ROV Jason dive number) when the FLOCS was deployed; values of IODP Expedition 336, MSM37 (R/V Maria S. Merian cruise MSM37) + J2-#### (ROV Jason dive number)
attribute FLOCSdeploymentCruise long_name String FLOCSdeployment Cruise
attribute FLOCSdeploymentCruise units String unitless
variable FLOCSrecoverCruise String
attribute FLOCSrecoverCruise bcodmo_name String cruise_id
attribute FLOCSrecoverCruise description String Name of the cruise (and ROV Jason dive number) when the FLOCS was recovered; values of AT39-01 (R/V Atlantis cruise AT39-01) + J2-#### (ROV Jason dive number)
attribute FLOCSrecoverCruise long_name String FLOCSrecover Cruise
attribute FLOCSrecoverCruise units String unitless
variable FluidcollectionCruise String
attribute FluidcollectionCruise bcodmo_name String cruise_id
attribute FluidcollectionCruise description String Name of the cruise and ROV Jason dive number when the crustal fluid was collected; values of AT39-01 (R/V Atlantis cruise AT39-01) + J2-#### (ROV Jason dive number)
attribute FluidcollectionCruise long_name String Fluidcollection Cruise
attribute FluidcollectionCruise units String unitless
variable FLOCSdeployDate String
attribute FLOCSdeployDate bcodmo_name String date
attribute FLOCSdeployDate description String Date of the FLOCS deployment, in ISO 8601 format yyyy-mm-dd
attribute FLOCSdeployDate long_name String FLOCSdeploy Date
attribute FLOCSdeployDate nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/ (external link)
attribute FLOCSdeployDate source_name String FLOCSdeployDate
attribute FLOCSdeployDate time_precision String 1970-01-01
attribute FLOCSdeployDate units String unitless
variable FLOCSrecoverDate String
attribute FLOCSrecoverDate bcodmo_name String date
attribute FLOCSrecoverDate description String Date of the FLOCS recovery, in ISO 8601 format yyyy-mm-dd
attribute FLOCSrecoverDate long_name String FLOCSrecover Date
attribute FLOCSrecoverDate nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/ (external link)
attribute FLOCSrecoverDate time_precision String 1970-01-01
attribute FLOCSrecoverDate units String unitless
variable FluidcollectionDate String
attribute FluidcollectionDate bcodmo_name String date
attribute FluidcollectionDate description String Date of the collection of the crustal fluids, in ISO 8601 format yyyy-mm-dd
attribute FluidcollectionDate long_name String Fluidcollection Date
attribute FluidcollectionDate nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/ (external link)
attribute FluidcollectionDate time_precision String 1970-01-01
attribute FluidcollectionDate units String unitless

 
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