BCO-DMO ERDDAP
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Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Natural seawater was enriched for photoautotrophs and split into multiple\ntemperatures for two weeks. After the enrichment period, Synechococcus was\nisolated from each temperature. Each isolate's thermal niche was measured\nthrough a series of lab experiments and sequenced.\n \n200ml of dense culture was concentrated by centrifuging 25,000 x g for 15\nminutes. The subsequent cell pellet was resuspended in 5ml of sterile growth\nmedia. Cell concentrate was dark acclimated for 10 minutes and 200ul was used\nto measure the fluorescence spectra. After each measurement, the temperature\nincreased and cells were dark acclimated at that temperature for an additional\n10 minutes. This continued until the fluorescence decreased to ~0 indicating\nthe disassociation of the photosynthetic apparatus. This was done following\nthe methods published in Pittera et al., 2017.
attribute NC_GLOBAL awards_0_award_nid String 712792
attribute NC_GLOBAL awards_0_award_number String OCE-1638804
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1638804 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_0_program_manager_nid String 50446
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Fluorescence spectra \n      for 3 strains of newly isolated marine Synechococcus distinct genotypes  \n      related to their accessory pigments while temperatures were increased to detect \n      the photosystem components disassociation temperature. \n   PI: D. Hutchins (USC) \n   version date: 2019-11-20
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3  19 Dec 2019
attribute NC_GLOBAL dataset_current_state String Final and no updates
attribute NC_GLOBAL date_created String 2019-11-21T14:03:29Z
attribute NC_GLOBAL date_modified String 2020-03-09T13:35:16Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.782322.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/782322 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to measure the fluorescence spectra
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 783245
attribute NC_GLOBAL instruments_0_description String Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: https://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.
attribute NC_GLOBAL instruments_0_instrument_name String plate reader
attribute NC_GLOBAL instruments_0_instrument_nid String 528693
attribute NC_GLOBAL instruments_0_supplied_name String SpectraMax m2 (Molecular Devices, CA)
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Used to create a pellet of cells.
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 783248
attribute NC_GLOBAL instruments_1_description String A machine with a rapidly rotating container that applies centrifugal force to its contents, typically to separate fluids of different densities (e.g., cream from milk) or liquids from solids.
attribute NC_GLOBAL instruments_1_instrument_name String Centrifuge
attribute NC_GLOBAL instruments_1_instrument_nid String 629890
attribute NC_GLOBAL instruments_1_supplied_name String centrifuge
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, chemical, data, dataset, dmo, Em_nm, erddap, la126, LA126_A, LA126_B, LA126_C, la127, LA127_A, LA127_B, LA127_C, la31, LA31_A, LA31_B, LA31_C, management, oceanography, office, preliminary, temperature
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/782322/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/782322 (external link)
attribute NC_GLOBAL param_mapping String {'782322': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/782322/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Southern California
attribute NC_GLOBAL people_0_affiliation_acronym String USC
attribute NC_GLOBAL people_0_person_name String David A. Hutchins
attribute NC_GLOBAL people_0_person_nid String 51048
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_1_person_name String Nancy Copley
attribute NC_GLOBAL people_1_person_nid String 50396
attribute NC_GLOBAL people_1_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL project String Phytoplankton Community Responses
attribute NC_GLOBAL projects_0_acronym String Phytoplankton Community Responses
attribute NC_GLOBAL projects_0_description String NSF Award Abstract:\nPhotosynthetic marine microbes, phytoplankton, contribute half of global primary production, form the base of most aquatic food webs and are major players in global biogeochemical cycles. Understanding their community composition is important because it affects higher trophic levels, the cycling of energy and elements and is sensitive to global environmental change. This project will investigate how phytoplankton communities respond to two major global change stressors in aquatic systems: warming and changes in nutrient availability. The researchers will work in two marine systems with a long history of environmental monitoring, the temperate Narragansett Bay estuary in Rhode Island and a subtropical North Atlantic site near Bermuda. They will use field sampling and laboratory experiments with multiple species and varieties of phytoplankton to assess the diversity in their responses to different temperatures under high and low nutrient concentrations. If the diversity of responses is high within species, then that species may have a better chance to adapt to rising temperatures and persist in the future. Some species may already be able to grow at high temperatures; consequently, they may become more abundant as the ocean warms. The researchers will incorporate this response information in mathematical models to predict how phytoplankton assemblages would reorganize under future climate scenarios. Graduate students and postdoctoral associates will be trained in diverse scientific approaches and techniques such as shipboard sampling, laboratory experiments, genomic analyses and mathematical modeling. The results of the project will be incorporated into K-12 teaching, including an advanced placement environmental science class for underrepresented minorities in Los Angeles, data exercises for rural schools in Michigan and disseminated to the public through an environmental journalism institute based in Rhode Island.\nPredicting how ecological communities will respond to a changing environment requires knowledge of genetic, phylogenetic and functional diversity within and across species. This project will investigate how the interaction of phylogenetic, genetic and functional diversity in thermal traits within and across a broad range of species determines the responses of marine phytoplankton communities to rising temperature and changing nutrient regimes. High genetic and functional diversity within a species may allow evolutionary adaptation of that species to warming. If the phylogenetic and functional diversity is higher across species, species sorting and ecological community reorganization is likely. Different marine sites may have a different balance of genetic and functional diversity within and across species and, thus, different contribution of evolutionary and ecological responses to changing climate. The research will be conducted at two long-term time series sites in the Atlantic Ocean, the Narragansett Bay Long-Term Plankton Time Series and the Bermuda Atlantic Time Series (BATS) station. The goal is to assess intra- and inter-specific genetic and functional diversity in thermal responses at contrasting nutrient concentrations for a representative range of species in communities at the two sites in different seasons, and use this information to parameterize eco-evolutionary models embedded into biogeochemical ocean models to predict responses of phytoplankton communities to projected rising temperatures under realistic nutrient conditions. Model predictions will be informed by and tested with field data, including the long-term data series available for both sites and in community temperature manipulation experiments. This project will provide novel information on existing intraspecific genetic and functional thermal diversity for many ecologically and biogeochemically important phytoplankton species, estimate generation of new genetic and functional diversity in evolution experiments, and develop and parameterize novel eco-evolutionary models interfaced with ocean biogeochemical models to predict future phytoplankton community structure. The project will also characterize the interaction of two major global change stressors, warming and changing nutrient concentrations, as they affect phytoplankton diversity at functional, genetic, and phylogenetic levels. In addition, the project will develop novel modeling methodology that will be broadly applicable to understanding how other types of complex ecological communities may adapt to a rapidly warming world.
attribute NC_GLOBAL projects_0_end_date String 2020-09
attribute NC_GLOBAL projects_0_geolocation String Narragansett Bay, RI and Bermuda, Bermuda Atlantic Time-series Study (BATS)
attribute NC_GLOBAL projects_0_name String Dimensions: Collaborative Research: Genetic, functional and phylogenetic diversity determines marine phytoplankton community responses to changing temperature and nutrients
attribute NC_GLOBAL projects_0_project_nid String 712787
attribute NC_GLOBAL projects_0_start_date String 2016-10
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Fluorescence spectra from 600-700nm at 530nm excitation for 3 strains of Synechococcus while increasing temperatures to detect the photosystem components disassociation temperature.
attribute NC_GLOBAL title String [Synechococcus fluorescence emission spectra] - Fluorescence spectra for 3 strains of Synechococcus while increasing temperatures to detect the photosystem components disassociation temperature (Dimensions: Collaborative Research: Genetic, functional and phylogenetic diversity determines marine phytoplankton community responses to changing temperature and nutrients)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.5
variable Temperature byte
attribute Temperature _FillValue byte 127
attribute Temperature actual_range byte 22, 57
attribute Temperature bcodmo_name String temperature
attribute Temperature description String experimental temperature
attribute Temperature long_name String Temperature
attribute Temperature nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/ (external link)
attribute Temperature units String degrees Celsius
variable Em_nm short
attribute Em_nm _FillValue short 32767
attribute Em_nm actual_range short 550, 714
attribute Em_nm bcodmo_name String wavelength
attribute Em_nm description String Experimental wavelength
attribute Em_nm long_name String Em Nm
attribute Em_nm units String nanometers
variable LA31_A float
attribute LA31_A _FillValue float NaN
attribute LA31_A actual_range float -832.303, 439.577
attribute LA31_A bcodmo_name String fluorescence
attribute LA31_A description String Percentage change in fluorescence emission at 530nm excitation for strain LA31 subsample A
attribute LA31_A long_name String LA31 A
attribute LA31_A nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/ (external link)
attribute LA31_A units String percent
variable LA31_B float
attribute LA31_B _FillValue float NaN
attribute LA31_B actual_range float -621.302, 450.052
attribute LA31_B bcodmo_name String fluorescence
attribute LA31_B description String Percentage change in fluorescence emission at 530nm excitation for strain LA31 subsample B
attribute LA31_B long_name String LA31 B
attribute LA31_B nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/ (external link)
attribute LA31_B units String percent
variable LA31_C float
attribute LA31_C _FillValue float NaN
attribute LA31_C actual_range float -31.349, 523.538
attribute LA31_C bcodmo_name String fluorescence
attribute LA31_C description String Percentage change in fluorescence emission at 530nm excitation for strain LA31 subsample C
attribute LA31_C long_name String LA31 C
attribute LA31_C nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/ (external link)
attribute LA31_C units String percent
variable LA126_A float
attribute LA126_A _FillValue float NaN
attribute LA126_A actual_range float -1046.895, 456.057
attribute LA126_A bcodmo_name String fluorescence
attribute LA126_A description String Percentage change in fluorescence emission at 530nm excitation for strain LA126 subsample A
attribute LA126_A long_name String LA126 A
attribute LA126_A nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/ (external link)
attribute LA126_A units String percent
variable LA126_B float
attribute LA126_B _FillValue float NaN
attribute LA126_B actual_range float -9.916, 571.553
attribute LA126_B bcodmo_name String fluorescence
attribute LA126_B description String Percentage change in fluorescence emission at 530nm excitation for strain LA126 subsample B
attribute LA126_B long_name String LA126 B
attribute LA126_B nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/ (external link)
attribute LA126_B units String percent
variable LA126_C double
attribute LA126_C _FillValue double NaN
attribute LA126_C actual_range double -11.731, 13308.723
attribute LA126_C bcodmo_name String fluorescence
attribute LA126_C description String Percentage change in fluorescence emission at 530nm excitation for strain LA126 subsample C
attribute LA126_C long_name String LA126 C
attribute LA126_C nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/ (external link)
attribute LA126_C units String percent
variable LA127_A float
attribute LA127_A _FillValue float NaN
attribute LA127_A actual_range float -13.41, 5481.022
attribute LA127_A bcodmo_name String fluorescence
attribute LA127_A description String Percentage change in fluorescence emission at 530nm excitation for strain LA127 subsample A
attribute LA127_A long_name String LA127 A
attribute LA127_A nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/ (external link)
attribute LA127_A units String percent
variable LA127_B float
attribute LA127_B _FillValue float NaN
attribute LA127_B actual_range float -1068.847, 375.704
attribute LA127_B bcodmo_name String fluorescence
attribute LA127_B description String Percentage change in fluorescence emission at 530nm excitation for strain LA127 subsample B
attribute LA127_B long_name String LA127 B
attribute LA127_B nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/ (external link)
attribute LA127_B units String percent
variable LA127_C double
attribute LA127_C _FillValue double NaN
attribute LA127_C actual_range double -14.4, 16090.617
attribute LA127_C bcodmo_name String fluorescence
attribute LA127_C description String Percentage change in fluorescence emission at 530nm excitation for strain LA127 subsample C
attribute LA127_C long_name String LA127 C
attribute LA127_C nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/ (external link)
attribute LA127_C units String percent

 
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