BCO-DMO ERDDAP
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Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String These samples were collected according to the protocol published by Krediet et\nal. 2015: Article Rapid, Precise, and Accurate Counts of Symbiodinium Cells\nUsing the Guava Flow Cytometer, and a Comparison to Other Methods\n \nThe samples that were analyzed for this study are 400 colonies of Acropora\nhyacinthus. Nubbins from each colony were experimentally bleached at\ntemperatures of 34, 34.5 and 35 degrees Celsius as well as two control\ntreatments (30 deg. C). These nubbins were then airbrushed and the resulting\ntissue slurry was preserved in RNAlater. Tissue dissociation and preparation\nfor analysis on the Guava EasyCyte flow cytometer followed the protocol by\nKrediet et al. 2015.\n \nProcedures followed Krediet et al. 2015 (above), with the exception that\nprotein concentration was not measured.
attribute NC_GLOBAL awards_0_award_nid String 764076
attribute NC_GLOBAL awards_0_award_number String OCE-1736736
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1736736 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Flowcyt \n  Stephen Palumbi  \n  Data Version 1: 2020-06-23
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3  19 Dec 2019
attribute NC_GLOBAL dataset_current_state String Final and no updates
attribute NC_GLOBAL date_created String 2020-05-28T16:36:32Z
attribute NC_GLOBAL date_modified String 2020-07-08T18:02:32Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.813210.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/813210 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Flow Cytometer
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 813213
attribute NC_GLOBAL instruments_0_description String Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.\n(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB37/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Flow Cytometer
attribute NC_GLOBAL instruments_0_instrument_nid String 660
attribute NC_GLOBAL instruments_0_supplied_name String Guava easyCyte HT 2-laser flow cytometer (Millipore)
attribute NC_GLOBAL keywords String acquisition, Acquisition_Time, allevents, ALLEVENTS_Conc, bco, bco-dmo, biological, cell, Cell_Count, cells, chemical, conc, count, data, dataset, dmo, erddap, events, management, negative, Negative_Acquisition_Time, Negative_Cells, Negative_Events, Negative_Sym_Conc, Negative_Total_Count, Negative_Total_Vol, number, Number_of_Events, oceanography, office, preliminary, sample, Sample_ID, sym, symbiodinium, SYMBIODINIUM_Conc, temperature, time, total, Total_Volume, treatment, vol, volume
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/813210/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/813210 (external link)
attribute NC_GLOBAL param_mapping String {'813210': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/813210/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Stanford University
attribute NC_GLOBAL people_0_person_name String Stephen R. Palumbi
attribute NC_GLOBAL people_0_person_nid String 51368
attribute NC_GLOBAL people_0_role String Lead Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_1_person_name String Christina Haskins
attribute NC_GLOBAL people_1_person_nid String 746212
attribute NC_GLOBAL people_1_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL project String Heat Tolerant Corals
attribute NC_GLOBAL projects_0_acronym String Heat Tolerant Corals
attribute NC_GLOBAL projects_0_description String NSF Award Abstract:\nWhen coral reefs heat up just a few degrees above normal summer temperatures, a reaction called coral bleaching can occur in which single celled plants living inside coral cells are expelled. The coral turns from its normal tan color to bleached white, and because it is deprived of the normal food supply from its plant partner, most of these corals die. Yet, some corals naturally can survive high temperatures that cause others in the same species to bleach. Identifying where these heat tolerant corals are common would provide a general tool for protecting and restoring heat tolerant reefs. The investigators will conduct experiments on 30 patch reefs in Palau of very different sizes in two lagoons, record local temperatures for 400 corals, and test coral heat tolerance using a newly designed coral stress tank. Because large patch reefs generally heat up during daytime low tides, The investigators hypothesize that they are commonly home to heat resistant corals. They will also move heat tolerant corals to cooler locations to test the stability of heat resistance among corals. The stress tank technologies can be widely used in remote settings, and will provide a set of generalizable, practical tools for communities and managers to find and protect heat tolerant corals in reefs around the world. The work will advance undergraduate STEM education in California and Palau. A partnership with the Palau Community College will facilitate the engagement of Pacific Island communities and students. Students will receive interdisciplinary training in field research, genomics and bioinformatics and learn practical skills that will enable them to collect and interpret stress tank and temperature data. Broader outreach efforts will include the production and dissemination of a series of microdocumentaries and blog posts designed to bring the concept of a world-wide search for heat tolerant corals to a global audience.\nPrevious coral reef research has demonstrated that periodic high water temperatures can induce high heat tolerance in reef building corals through a combination of acclimation and selection at many genetic loci. Key questions include whether these kinds of heat tolerant habitats are common or rare, and whether their locations can be predicted by identifying coral reefs where daily temperature spikes regularly occur at low tide. This project will examine heat tolerance of 400 corals in the Acropora hyacinthus species complex across 30 patch reefs in Palau that experience variable temperature and flow profiles. This study will utilize a variety of methods to characterize spatial and temporal patterns of heat tolerance including: (1) the development of low-cost, portable heat stress tanks to quickly and affordably assess in situ conditions, (2) genomic assays of physiological condition to identify the genes and gene expression mechanisms that are responsible for heat tolerance, (3) high resolution temperature mapping to trace the role of temperature variation in producing stable, high temperature tolerance in reef building corals, and (4) reciprocal transplant experiments to evaluate whether heat resistant corals retain heat resistance when moved to cooler locations. This research will expand the geographic map of habitats with known heat tolerance, and expedite the ability to locate coral populations that may be most resistant to future ocean warming.
attribute NC_GLOBAL projects_0_end_date String 2020-09
attribute NC_GLOBAL projects_0_geolocation String Palau
attribute NC_GLOBAL projects_0_name String Predicting the global location of heat tolerant corals: Palau patch reefs as a general model
attribute NC_GLOBAL projects_0_project_nid String 764077
attribute NC_GLOBAL projects_0_start_date String 2017-10
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Results of the quantification of symbiont cell numbers from 400 Acropora hyacinthus colonies subjected to experimental bleaching in the summer of 2018 in Palau.
attribute NC_GLOBAL title String [Flow Cytometry Results] - Results of the quantification of symbiont cell numbers from 400 Acropora hyacinthus colonies subjected to experimental bleaching in the summer of 2018 in Palau. (Predicting the global location of heat tolerant corals: Palau patch reefs as a general model)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.5
variable Sample short
attribute Sample _FillValue short 32767
attribute Sample actual_range short 1, 400
attribute Sample bcodmo_name String sample
attribute Sample description String Colony Number
attribute Sample long_name String Sample
attribute Sample nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute Sample units String dimensionless
variable Treatment String
attribute Treatment bcodmo_name String treatment
attribute Treatment description String Treatment (C1 and C2 are control), all others are heat stress
attribute Treatment long_name String Treatment
attribute Treatment units String unitless
variable Temperature float
attribute Temperature _FillValue float NaN
attribute Temperature actual_range float 30.0, 35.0
attribute Temperature bcodmo_name String temperature
attribute Temperature description String Specific Temperature treatment
attribute Temperature long_name String Temperature
attribute Temperature units String degrees Celsius
variable Sample_ID short
attribute Sample_ID _FillValue short 32767
attribute Sample_ID actual_range short 1, 1684
attribute Sample_ID bcodmo_name String sample
attribute Sample_ID description String Unique sample ID (technical replicates have the same SampleID)
attribute Sample_ID long_name String Sample ID
attribute Sample_ID nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute Sample_ID units String dimensionless
variable ALLEVENTS_Conc double
attribute ALLEVENTS_Conc _FillValue double NaN
attribute ALLEVENTS_Conc actual_range double 2096.96, 2541437.63
attribute ALLEVENTS_Conc bcodmo_name String cell_concentration
attribute ALLEVENTS_Conc description String Concentration (cells/ml) of events above gating threshold
attribute ALLEVENTS_Conc long_name String ALLEVENTS Conc
attribute ALLEVENTS_Conc units String cells/ml
variable SYMBIODINIUM_Conc double
attribute SYMBIODINIUM_Conc _FillValue double NaN
attribute SYMBIODINIUM_Conc actual_range double 0.0, 396300.22
attribute SYMBIODINIUM_Conc bcodmo_name String cell_concentration
attribute SYMBIODINIUM_Conc description String Concentration (cells/ml) of events above gating threshold that are also above the symbiodinium gating threshold
attribute SYMBIODINIUM_Conc long_name String SYMBIODINIUM Conc
attribute SYMBIODINIUM_Conc units String cells/ml
variable Number_of_Events short
attribute Number_of_Events _FillValue short 32767
attribute Number_of_Events actual_range short 30, 5000
attribute Number_of_Events bcodmo_name String count
attribute Number_of_Events colorBarMaximum double 100.0
attribute Number_of_Events colorBarMinimum double 0.0
attribute Number_of_Events description String Number of events counted (max 5000)
attribute Number_of_Events long_name String Number Of Events
attribute Number_of_Events units String unitless
variable Cell_Count float
attribute Cell_Count _FillValue float NaN
attribute Cell_Count actual_range float 2.1, 2541.44
attribute Cell_Count bcodmo_name String cell_concentration
attribute Cell_Count colorBarMaximum double 100.0
attribute Cell_Count colorBarMinimum double 0.0
attribute Cell_Count description String Concentration (cells/ul) of events above gating threshold
attribute Cell_Count long_name String Cell Count
attribute Cell_Count units String cells/ul
variable Total_Volume float
attribute Total_Volume _FillValue float NaN
attribute Total_Volume actual_range float 1.97, 100.3
attribute Total_Volume bcodmo_name String volume
attribute Total_Volume description String Total volume of sample measured in microliters
attribute Total_Volume long_name String Total Volume
attribute Total_Volume units String microliters
variable Acquisition_Time float
attribute Acquisition_Time _FillValue float NaN
attribute Acquisition_Time actual_range float 8.34, 425.01
attribute Acquisition_Time bcodmo_name String time_elapsed
attribute Acquisition_Time description String Total acquisition time on instrument in seconds
attribute Acquisition_Time long_name String Acquisition Time
attribute Acquisition_Time nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/ (external link)
attribute Acquisition_Time units String seconds
variable Negative_Total_Count short
attribute Negative_Total_Count _FillValue short 32767
attribute Negative_Total_Count actual_range short 22, 517
attribute Negative_Total_Count bcodmo_name String cell_concentration
attribute Negative_Total_Count colorBarMaximum double 100.0
attribute Negative_Total_Count colorBarMinimum double 0.0
attribute Negative_Total_Count description String Number of events above gating threshold in negative control
attribute Negative_Total_Count long_name String Negative Total Count
attribute Negative_Total_Count units String various
variable Negative_Events float
attribute Negative_Events _FillValue float NaN
attribute Negative_Events actual_range float 14.4, 36137.93
attribute Negative_Events bcodmo_name String cell_concentration
attribute Negative_Events description String Concentration of cells (cells/ml) in negative control above gating threshold
attribute Negative_Events long_name String Negative Events
attribute Negative_Events units String cells/ml
variable Negative_Sym_Conc float
attribute Negative_Sym_Conc _FillValue float NaN
attribute Negative_Sym_Conc actual_range float 0.0, 2935.75
attribute Negative_Sym_Conc bcodmo_name String cell_concentration
attribute Negative_Sym_Conc description String Concentration of symbiont cells in negative control above gating threshold
attribute Negative_Sym_Conc long_name String Negative Sym Conc
attribute Negative_Sym_Conc units String various
variable Negative_Cells float
attribute Negative_Cells _FillValue float NaN
attribute Negative_Cells actual_range float 1.54, 36.14
attribute Negative_Cells bcodmo_name String cell_concentration
attribute Negative_Cells description String Concentration of cells (cells/ul) in negative control above gating threshold
attribute Negative_Cells long_name String Negative Cells
attribute Negative_Cells units String cell/ul
variable Negative_Total_Vol float
attribute Negative_Total_Vol _FillValue float NaN
attribute Negative_Total_Vol actual_range float 14.3, 14.31
attribute Negative_Total_Vol bcodmo_name String volume
attribute Negative_Total_Vol description String Total volume of negative control measured in microliters
attribute Negative_Total_Vol long_name String Negative Total Vol
attribute Negative_Total_Vol units String microliters
variable Negative_Acquisition_Time float
attribute Negative_Acquisition_Time _FillValue float NaN
attribute Negative_Acquisition_Time actual_range float 60.6, 60.62
attribute Negative_Acquisition_Time bcodmo_name String time_elapsed
attribute Negative_Acquisition_Time description String Total acquisistion time on instrument for negative control in seconds
attribute Negative_Acquisition_Time long_name String Negative Acquisition Time
attribute Negative_Acquisition_Time nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/ (external link)
attribute Negative_Acquisition_Time units String seconds

 
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