BCO-DMO ERDDAP
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Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Location:\\u00a0North Atlantic, western flank of the mid-Atlantic\nRidge\\u00a022.75589 N 46.08125 W\n \nMethodology:\n \nPrior to the extraction, we freeze-dried, ground and sieved sediment samples\nto less than 125 \\u03bcm (Ruttenberg 1992). For a given sample, we weighed\nfour sample replicates (2 g) and placed each in 250 mL HDPE bottles. Sodium\ndithionite (F.W. 147.12 g/mol; 7.4 g) was added to each sample split, followed\nby 200 mL of citrate-bicarbonate solution (pH 7.6). This step produces\neffervescence, so the solution should be added slowly to the sample. We shook\nsamples for 8 h and then centrifuged them at 3,700 rpm for 15 min. We filtered\nthe supernatants with a 0.4 \\u03bcm polycarbonate filter. We took 20 mL\naliquots from the filtrate for each sample split for MRP and total P analyses,\nand kept them refrigerated until analysis within 24 h. We added 200 mL of\nultrapure water to the solid residue for each sample split as a wash step\nafter the above reductive step, shook samples for 2 h, and then centrifuged\nthem at 3,700 rpm for 15 min. We filtered the supernatants with 0.4 \\u03bcm\npolycarbonate filters and set aside 20 mL of filtrate from each sample split\nfor MRP and total P analyses. We then extracted the solid sample residues in\n200 mL of sodium acetate buffer (pH 4.0) for 6 h. At the end of this\nextraction step, we centrifuged the bottles at 3,700 rpm for 15 min, filtered\nthe supernatants with 0.4 \\u03bcm polycarbonate filters and took a 20 mL\naliquot of filtrate from each sample split for MRP and total P analyses. We\nadded 200 mL of ultrapure water to the solid residue for each sample split as\na wash step, shook samples for 2 h, and then centrifuged them at 3,700 rpm for\n15 min.\\u00a0 We filtered the supernatants with 0.4 \\u03bcm polycarbonate\nfilters and set aside 20 mL of filtrate from each sample split for MRP and\ntotal P analyses. We repeated the water rinse step, and collected aliquots for\nMRP and total P analyses as in the previous steps. The concentrations of\\u00a0\nTP were determined as described below.\n \nSolid sediment sample residues following the pretreatment described above were\ntransferred to two 50 mL centrifuge tubes (2 sample replicates combined per\ntube). We added 20 mL of 0.25 M NaOH + 0.05 M Na2EDTA solution to each tube,\nvortexed until all sediment was resuspended and then shook samples for 6 h at\nroom temperature (Cade-Menun et al. 2005). We used a solid to solution ratio\nof 1:5 for this step to minimize the amount of freeze-dried material that will\nneed to be dissolved for the 31P NMR experiments. Large amounts of salts from\nthe NaOH-EDTA concentrated in NMR samples lead to higher viscosity and\nincrease line broadening on NMR spectra (Cade-Menun and Liu 2014). We chose an\nextraction time of 6 h to improve total P recovery while limiting the\ndegradation of natural P compounds in the sample. At the end of the\nextraction, samples were centrifuged at 3,700 rpm for 15 min and supernatants\ndecanted into 50 mL centrifuge tubes. We collected a 500 \\u03bcL aliquot from\neach sample, which we diluted with 4.5 mL of ultrapure water. These were\nrefrigerated until analysis for total P content on the ICP-OES. The sample\nresidues and supernatants were frozen on a slant to maximize the exposed\nsurface area during the lyophilization step; this was done immediately after\nthe removal of the 500 \\u03bcL aliquot. Once completely frozen, the uncapped\ntubes containing supernatants and residues were freeze-dried over the course\nof 48 h. Each tube was covered with parafilm with small holes from a tack to\nminimize contamination. Freeze-dried supernatants from identical sample splits\nwere combined and dissolved in 500 \\u03bcL each of ultrapure water, D2O, NaOH-\nEDTA and 10 M NaOH prior to 31P NMR analysis.\\u00a0 The D2O is required as\nsignal lock in the spectrometer (Cade-Menun and Liu 2014). Sample pH was\nmaintained at a pH > 12 to optimize peak separation (Cade-Menun 2005; Cade-\nMenun and Liu 2014). Sample pH was assessed with a glass electrode, and\nverified with pH paper to account for the alkaline error caused by the high\nsalt content of our samples (Covington 1985).\n \nFreeze-dried sample residues were ashed in crucibles at 550oC for 2 h and then\nextracted in 25 mL of 0.5 M sulfuric acid for 16 h (Olsen and Sommers 1982;\nCade-Menun and Lavkulich 1997). We centrifuged samples at 3,700 rpm for 15\nmin, filtered supernatants with 0.4 \\u03bcm polycarbonate filters, and\nmeasured P content on an ICP-OES.\\u00a0\n \nTotal P concentrations in sediment extracts were measured using inductively\ncoupled plasma optical emission spectroscopy (ICP-OES). Standards were\nprepared with the same solutions as those used for the extraction procedure in\norder to minimize matrix effects on P measurements. Sediment extracts and\nstandards (0 \\u03bcM, 3.2 \\u03bcM, 32 \\u03bcM and 320 \\u03bcM) were diluted to\nlower salt content to prevent salt buildup on the nebulizer (1:20 dilution for\nstep 1, 1:10 for steps 2 \\u2013 4). Concentration data from both wavelengths\n(213 nm and 214 nm) were averaged to obtain extract concentrations for each\nsample. The detection limit for P on this instrument for both wavelengths is\n0.4 \\u03bcM. The MRP concentrations were measured on a QuikChem 8000 automated\nion analyzer. Standards were prepared with the same solutions used for the\nextraction step to minimize matrix effects on P measurements. Sediment\nextracts and standards (0 \\u2013 30 \\u03bcM PO4) were diluted ten-fold to\nprevent matrix interference with color development. The detection limit for P\non this instrument is 0.2 \\u03bcM. We derived MUP concentrations by\nsubtracting MRP from total P concentrations.
attribute NC_GLOBAL awards_0_award_nid String 554980
attribute NC_GLOBAL awards_0_award_number String OCE-0939564
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward?AWD_ID=0939564 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Iodp336-tp-sed-pre \n  PI: Adina Paytan \n  Data Version 1: 2020-06-23
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3  19 Dec 2019
attribute NC_GLOBAL dataset_current_state String Final and no updates
attribute NC_GLOBAL date_created String 2020-06-23T18:47:07Z
attribute NC_GLOBAL date_modified String 2020-06-30T18:11:16Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.816576.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/816576 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String ICP-OES
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 816583
attribute NC_GLOBAL instruments_0_description String Also referred to as an Inductively coupled plasma atomic emission spectroscope (ICP-AES). These instruments pass nebulised samples into an inductively-coupled gas plasma (8-10000 K) where they are atomised and excited. The de-excitation optical emissions at characteristic wavelengths are spectroscopically analysed. It is often used in the detection of trace metals.
attribute NC_GLOBAL instruments_0_instrument_name String Inductively Coupled Plasma Optical Emission Spectrometer
attribute NC_GLOBAL instruments_0_instrument_nid String 639924
attribute NC_GLOBAL instruments_0_supplied_name String ICP-OES
attribute NC_GLOBAL keywords String analyte, Analyte_Name, bco, bco-dmo, biological, calib, chemical, conc, Conc_Calib, corr, data, dataset, date, dilution, dmo, erddap, extract, int, Int_Corr, management, name, oceanography, office, preliminary, rsd, RSD_Corr_Int, sample, Sample_ID, step, time
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/816576/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/816576 (external link)
attribute NC_GLOBAL param_mapping String {'816576': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/816576/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of California-Santa Cruz
attribute NC_GLOBAL people_0_affiliation_acronym String UC Santa Cruz
attribute NC_GLOBAL people_0_person_name String Adina Paytan
attribute NC_GLOBAL people_0_person_nid String 50821
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String University of California-Santa Cruz
attribute NC_GLOBAL people_1_affiliation_acronym String UC Santa Cruz
attribute NC_GLOBAL people_1_person_name String Dr Delphine Defforey
attribute NC_GLOBAL people_1_person_nid String 664058
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_2_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_2_person_name String Amber D. York
attribute NC_GLOBAL people_2_person_nid String 643627
attribute NC_GLOBAL people_2_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL project String Deep sea sediments
attribute NC_GLOBAL projects_0_acronym String Deep sea sediments
attribute NC_GLOBAL projects_0_description String The goal of this project is to explore potential microbial P uptake mechanisms in marine sediments beneath the North Atlantic Gyre and their effects on the relative distribution of organic P compounds as a function of burial depth and changing redox conditions. We use a combination of metagenomic analyses and solution 31P nuclear magnetic resonance spectroscopy (31P NMR) to investigate (1) the presence of microbial functional genes pertaining to P uptake and metabolism and (2) the possible P substrates for the deep biosphere in these oligotrophic sediments.\nNSF C-DEBI Award #156246 to Dr. Adina Paytan\nNSF C-DEBI Award #157598 to Dr. Delphine Defforey
attribute NC_GLOBAL projects_0_end_date String 2015-04
attribute NC_GLOBAL projects_0_geolocation String Mid-Atlantic Ridge flank
attribute NC_GLOBAL projects_0_name String Potential phosphorus uptake mechanisms of the deep sedimentary biosphere
attribute NC_GLOBAL projects_0_project_nid String 664073
attribute NC_GLOBAL projects_0_start_date String 2015-03
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Total phosphorus concentrations in nuclear magnetic resonance (NMR) sediment pretreatment extracts from samples collected during R/V JOIDES Resolution cruise JRES-336 (IODP336, North Pond) to the western flank of the mid-Atlantic Ridge in November of 2011. Samples were analyzed in 2016.
attribute NC_GLOBAL title String [TP sediments with pretreatment] - Total phosphorus concentrations in NMR sediment pretreatment extracts from samples collected during R/V JOIDES Resolution cruise JRES-336 (IODP336, North Pond) to the western flank of the mid-Atlantic Ridge in November of 2011 (Potential phosphorus uptake mechanisms of the deep sedimentary biosphere)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.5
variable Extract String
attribute Extract bcodmo_name String sample_descrip
attribute Extract description String Extract solution
attribute Extract long_name String Extract
attribute Extract units String unitless
variable Step String
attribute Step bcodmo_name String sample_descrip
attribute Step description String Step in the sequential extraction scheme (1-4)
attribute Step long_name String Step
attribute Step units String unitless
variable Dilution String
attribute Dilution bcodmo_name String sample_descrip
attribute Dilution description String Sample dilution
attribute Dilution long_name String Dilution
attribute Dilution units String unitless
variable Sample_ID String
attribute Sample_ID bcodmo_name String sample
attribute Sample_ID description String Sample ID, unique sample identifier
attribute Sample_ID long_name String Sample ID
attribute Sample_ID nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute Sample_ID units String unitless
variable Date String
attribute Date bcodmo_name String date
attribute Date description String Date the samples were analyzed in ISO 8601 format yyyy-mm-dd
attribute Date long_name String Date
attribute Date nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/ (external link)
attribute Date source_name String Date
attribute Date time_precision String 1970-01-01
attribute Date units String nd
variable Analyte_Name String
attribute Analyte_Name bcodmo_name String sample_descrip
attribute Analyte_Name description String Element analyzed
attribute Analyte_Name long_name String Analyte Name
attribute Analyte_Name units String unitless
variable Int_Corr double
attribute Int_Corr _FillValue double NaN
attribute Int_Corr actual_range double -8736.84, 3.163645165E7
attribute Int_Corr bcodmo_name String unknown
attribute Int_Corr description String Intensity (corrected)
attribute Int_Corr long_name String Int Corr
attribute Int_Corr units String unitless
variable RSD_Corr_Int float
attribute RSD_Corr_Int _FillValue float NaN
attribute RSD_Corr_Int actual_range float 0.14, 1383.25
attribute RSD_Corr_Int bcodmo_name String unknown
attribute RSD_Corr_Int description String Relative standard deviation (RSD) of corrected intensity
attribute RSD_Corr_Int long_name String RSD Corr Int
attribute RSD_Corr_Int units String unitless
variable Conc_Calib float
attribute Conc_Calib _FillValue float NaN
attribute Conc_Calib actual_range float -0.2, 122.54
attribute Conc_Calib bcodmo_name String P
attribute Conc_Calib description String Calibrated concentration of total phosphorous
attribute Conc_Calib long_name String Conc Calib
attribute Conc_Calib units String parts per million (ppm)

 
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