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Row Type | Variable Name | Attribute Name | Data Type | Value |
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attribute | NC_GLOBAL | access_formats | String | .htmlTable,.csv,.json,.mat,.nc,.tsv |
attribute | NC_GLOBAL | acquisition_description | String | Location:\\u00a0North Atlantic, western flank of the mid-Atlantic\nRidge\\u00a022.75589 N 46.08125 W\n \nMethodology:\n \nPrior to the extraction, we freeze-dried, ground and sieved sediment samples\nto less than 125 \\u03bcm (Ruttenberg 1992). For a given sample, we weighed\nfour sample replicates (2 g) and placed each in 250 mL HDPE bottles. Sodium\ndithionite (F.W. 147.12 g/mol; 7.4 g) was added to each sample split, followed\nby 200 mL of citrate-bicarbonate solution (pH 7.6). This step produces\neffervescence, so the solution should be added slowly to the sample. We shook\nsamples for 8 h and then centrifuged them at 3,700 rpm for 15 min. We filtered\nthe supernatants with a 0.4 \\u03bcm polycarbonate filter. We took 20 mL\naliquots from the filtrate for each sample split for MRP and total P analyses,\nand kept them refrigerated until analysis within 24 h. We added 200 mL of\nultrapure water to the solid residue for each sample split as a wash step\nafter the above reductive step, shook samples for 2 h, and then centrifuged\nthem at 3,700 rpm for 15 min. We filtered the supernatants with 0.4 \\u03bcm\npolycarbonate filters and set aside 20 mL of filtrate from each sample split\nfor MRP and total P analyses. We then extracted the solid sample residues in\n200 mL of sodium acetate buffer (pH 4.0) for 6 h. At the end of this\nextraction step, we centrifuged the bottles at 3,700 rpm for 15 min, filtered\nthe supernatants with 0.4 \\u03bcm polycarbonate filters and took a 20 mL\naliquot of filtrate from each sample split for MRP and total P analyses. We\nadded 200 mL of ultrapure water to the solid residue for each sample split as\na wash step, shook samples for 2 h, and then centrifuged them at 3,700 rpm for\n15 min.\\u00a0 We filtered the supernatants with 0.4 \\u03bcm polycarbonate\nfilters and set aside 20 mL of filtrate from each sample split for MRP and\ntotal P analyses. We repeated the water rinse step, and collected aliquots for\nMRP and total P analyses as in the previous steps. The concentrations of\\u00a0\nTP were determined as described below.\n \nSolid sediment sample residues following the pretreatment described above were\ntransferred to two 50 mL centrifuge tubes (2 sample replicates combined per\ntube). We added 20 mL of 0.25 M NaOH + 0.05 M Na2EDTA solution to each tube,\nvortexed until all sediment was resuspended and then shook samples for 6 h at\nroom temperature (Cade-Menun et al. 2005). We used a solid to solution ratio\nof 1:5 for this step to minimize the amount of freeze-dried material that will\nneed to be dissolved for the 31P NMR experiments. Large amounts of salts from\nthe NaOH-EDTA concentrated in NMR samples lead to higher viscosity and\nincrease line broadening on NMR spectra (Cade-Menun and Liu 2014). We chose an\nextraction time of 6 h to improve total P recovery while limiting the\ndegradation of natural P compounds in the sample. At the end of the\nextraction, samples were centrifuged at 3,700 rpm for 15 min and supernatants\ndecanted into 50 mL centrifuge tubes. We collected a 500 \\u03bcL aliquot from\neach sample, which we diluted with 4.5 mL of ultrapure water. These were\nrefrigerated until analysis for total P content on the ICP-OES. The sample\nresidues and supernatants were frozen on a slant to maximize the exposed\nsurface area during the lyophilization step; this was done immediately after\nthe removal of the 500 \\u03bcL aliquot. Once completely frozen, the uncapped\ntubes containing supernatants and residues were freeze-dried over the course\nof 48 h. Each tube was covered with parafilm with small holes from a tack to\nminimize contamination. Freeze-dried supernatants from identical sample splits\nwere combined and dissolved in 500 \\u03bcL each of ultrapure water, D2O, NaOH-\nEDTA and 10 M NaOH prior to 31P NMR analysis.\\u00a0 The D2O is required as\nsignal lock in the spectrometer (Cade-Menun and Liu 2014). Sample pH was\nmaintained at a pH > 12 to optimize peak separation (Cade-Menun 2005; Cade-\nMenun and Liu 2014). Sample pH was assessed with a glass electrode, and\nverified with pH paper to account for the alkaline error caused by the high\nsalt content of our samples (Covington 1985).\n \nFreeze-dried sample residues were ashed in crucibles at 550oC for 2 h and then\nextracted in 25 mL of 0.5 M sulfuric acid for 16 h (Olsen and Sommers 1982;\nCade-Menun and Lavkulich 1997). We centrifuged samples at 3,700 rpm for 15\nmin, filtered supernatants with 0.4 \\u03bcm polycarbonate filters, and\nmeasured P content on an ICP-OES.\\u00a0\n \nTotal P concentrations in sediment extracts were measured using inductively\ncoupled plasma optical emission spectroscopy (ICP-OES). Standards were\nprepared with the same solutions as those used for the extraction procedure in\norder to minimize matrix effects on P measurements. Sediment extracts and\nstandards (0 \\u03bcM, 3.2 \\u03bcM, 32 \\u03bcM and 320 \\u03bcM) were diluted to\nlower salt content to prevent salt buildup on the nebulizer (1:20 dilution for\nstep 1, 1:10 for steps 2 \\u2013 4). Concentration data from both wavelengths\n(213 nm and 214 nm) were averaged to obtain extract concentrations for each\nsample. The detection limit for P on this instrument for both wavelengths is\n0.4 \\u03bcM. The MRP concentrations were measured on a QuikChem 8000 automated\nion analyzer. Standards were prepared with the same solutions used for the\nextraction step to minimize matrix effects on P measurements. Sediment\nextracts and standards (0 \\u2013 30 \\u03bcM PO4) were diluted ten-fold to\nprevent matrix interference with color development. The detection limit for P\non this instrument is 0.2 \\u03bcM. We derived MUP concentrations by\nsubtracting MRP from total P concentrations. |
attribute | NC_GLOBAL | awards_0_award_nid | String | 554980 |
attribute | NC_GLOBAL | awards_0_award_number | String | OCE-0939564 |
attribute | NC_GLOBAL | awards_0_data_url | String | http://www.nsf.gov/awardsearch/showAward?AWD_ID=0939564 |
attribute | NC_GLOBAL | awards_0_funder_name | String | NSF Division of Ocean Sciences |
attribute | NC_GLOBAL | awards_0_funding_acronym | String | NSF OCE |
attribute | NC_GLOBAL | awards_0_funding_source_nid | String | 355 |
attribute | NC_GLOBAL | awards_0_program_manager | String | David L. Garrison |
attribute | NC_GLOBAL | awards_0_program_manager_nid | String | 50534 |
attribute | NC_GLOBAL | cdm_data_type | String | Other |
attribute | NC_GLOBAL | comment | String | Iodp336-tp-sed-pre \n PI: Adina Paytan \n Data Version 1: 2020-06-23 |
attribute | NC_GLOBAL | Conventions | String | COARDS, CF-1.6, ACDD-1.3 |
attribute | NC_GLOBAL | creator_email | String | info at bco-dmo.org |
attribute | NC_GLOBAL | creator_name | String | BCO-DMO |
attribute | NC_GLOBAL | creator_type | String | institution |
attribute | NC_GLOBAL | creator_url | String | https://www.bco-dmo.org/ |
attribute | NC_GLOBAL | data_source | String | extract_data_as_tsv version 2.3 19 Dec 2019 |
attribute | NC_GLOBAL | dataset_current_state | String | Final and no updates |
attribute | NC_GLOBAL | date_created | String | 2020-06-23T18:47:07Z |
attribute | NC_GLOBAL | date_modified | String | 2020-06-30T18:11:16Z |
attribute | NC_GLOBAL | defaultDataQuery | String | &time<now |
attribute | NC_GLOBAL | doi | String | 10.26008/1912/bco-dmo.816576.1 |
attribute | NC_GLOBAL | infoUrl | String | https://www.bco-dmo.org/dataset/816576 |
attribute | NC_GLOBAL | institution | String | BCO-DMO |
attribute | NC_GLOBAL | instruments_0_acronym | String | ICP-OES |
attribute | NC_GLOBAL | instruments_0_dataset_instrument_nid | String | 816583 |
attribute | NC_GLOBAL | instruments_0_description | String | Also referred to as an Inductively coupled plasma atomic emission spectroscope (ICP-AES). These instruments pass nebulised samples into an inductively-coupled gas plasma (8-10000 K) where they are atomised and excited. The de-excitation optical emissions at characteristic wavelengths are spectroscopically analysed. It is often used in the detection of trace metals. |
attribute | NC_GLOBAL | instruments_0_instrument_name | String | Inductively Coupled Plasma Optical Emission Spectrometer |
attribute | NC_GLOBAL | instruments_0_instrument_nid | String | 639924 |
attribute | NC_GLOBAL | instruments_0_supplied_name | String | ICP-OES |
attribute | NC_GLOBAL | keywords | String | analyte, Analyte_Name, bco, bco-dmo, biological, calib, chemical, conc, Conc_Calib, corr, data, dataset, date, dilution, dmo, erddap, extract, int, Int_Corr, management, name, oceanography, office, preliminary, rsd, RSD_Corr_Int, sample, Sample_ID, step, time |
attribute | NC_GLOBAL | license | String | https://www.bco-dmo.org/dataset/816576/license |
attribute | NC_GLOBAL | metadata_source | String | https://www.bco-dmo.org/api/dataset/816576 |
attribute | NC_GLOBAL | param_mapping | String | {'816576': {}} |
attribute | NC_GLOBAL | parameter_source | String | https://www.bco-dmo.org/mapserver/dataset/816576/parameters |
attribute | NC_GLOBAL | people_0_affiliation | String | University of California-Santa Cruz |
attribute | NC_GLOBAL | people_0_affiliation_acronym | String | UC Santa Cruz |
attribute | NC_GLOBAL | people_0_person_name | String | Adina Paytan |
attribute | NC_GLOBAL | people_0_person_nid | String | 50821 |
attribute | NC_GLOBAL | people_0_role | String | Principal Investigator |
attribute | NC_GLOBAL | people_0_role_type | String | originator |
attribute | NC_GLOBAL | people_1_affiliation | String | University of California-Santa Cruz |
attribute | NC_GLOBAL | people_1_affiliation_acronym | String | UC Santa Cruz |
attribute | NC_GLOBAL | people_1_person_name | String | Dr Delphine Defforey |
attribute | NC_GLOBAL | people_1_person_nid | String | 664058 |
attribute | NC_GLOBAL | people_1_role | String | Co-Principal Investigator |
attribute | NC_GLOBAL | people_1_role_type | String | originator |
attribute | NC_GLOBAL | people_2_affiliation | String | Woods Hole Oceanographic Institution |
attribute | NC_GLOBAL | people_2_affiliation_acronym | String | WHOI BCO-DMO |
attribute | NC_GLOBAL | people_2_person_name | String | Amber D. York |
attribute | NC_GLOBAL | people_2_person_nid | String | 643627 |
attribute | NC_GLOBAL | people_2_role | String | BCO-DMO Data Manager |
attribute | NC_GLOBAL | people_2_role_type | String | related |
attribute | NC_GLOBAL | project | String | Deep sea sediments |
attribute | NC_GLOBAL | projects_0_acronym | String | Deep sea sediments |
attribute | NC_GLOBAL | projects_0_description | String | The goal of this project is to explore potential microbial P uptake mechanisms in marine sediments beneath the North Atlantic Gyre and their effects on the relative distribution of organic P compounds as a function of burial depth and changing redox conditions. We use a combination of metagenomic analyses and solution 31P nuclear magnetic resonance spectroscopy (31P NMR) to investigate (1) the presence of microbial functional genes pertaining to P uptake and metabolism and (2) the possible P substrates for the deep biosphere in these oligotrophic sediments.\nNSF C-DEBI Award #156246 to Dr. Adina Paytan\nNSF C-DEBI Award #157598 to Dr. Delphine Defforey |
attribute | NC_GLOBAL | projects_0_end_date | String | 2015-04 |
attribute | NC_GLOBAL | projects_0_geolocation | String | Mid-Atlantic Ridge flank |
attribute | NC_GLOBAL | projects_0_name | String | Potential phosphorus uptake mechanisms of the deep sedimentary biosphere |
attribute | NC_GLOBAL | projects_0_project_nid | String | 664073 |
attribute | NC_GLOBAL | projects_0_start_date | String | 2015-03 |
attribute | NC_GLOBAL | publisher_name | String | Biological and Chemical Oceanographic Data Management Office (BCO-DMO) |
attribute | NC_GLOBAL | publisher_type | String | institution |
attribute | NC_GLOBAL | sourceUrl | String | (local files) |
attribute | NC_GLOBAL | standard_name_vocabulary | String | CF Standard Name Table v55 |
attribute | NC_GLOBAL | summary | String | Total phosphorus concentrations in nuclear magnetic resonance (NMR) sediment pretreatment extracts from samples collected during R/V JOIDES Resolution cruise JRES-336 (IODP336, North Pond) to the western flank of the mid-Atlantic Ridge in November of 2011. Samples were analyzed in 2016. |
attribute | NC_GLOBAL | title | String | [TP sediments with pretreatment] - Total phosphorus concentrations in NMR sediment pretreatment extracts from samples collected during R/V JOIDES Resolution cruise JRES-336 (IODP336, North Pond) to the western flank of the mid-Atlantic Ridge in November of 2011 (Potential phosphorus uptake mechanisms of the deep sedimentary biosphere) |
attribute | NC_GLOBAL | version | String | 1 |
attribute | NC_GLOBAL | xml_source | String | osprey2erddap.update_xml() v1.5 |
variable | Extract | String | ||
attribute | Extract | bcodmo_name | String | sample_descrip |
attribute | Extract | description | String | Extract solution |
attribute | Extract | long_name | String | Extract |
attribute | Extract | units | String | unitless |
variable | Step | String | ||
attribute | Step | bcodmo_name | String | sample_descrip |
attribute | Step | description | String | Step in the sequential extraction scheme (1-4) |
attribute | Step | long_name | String | Step |
attribute | Step | units | String | unitless |
variable | Dilution | String | ||
attribute | Dilution | bcodmo_name | String | sample_descrip |
attribute | Dilution | description | String | Sample dilution |
attribute | Dilution | long_name | String | Dilution |
attribute | Dilution | units | String | unitless |
variable | Sample_ID | String | ||
attribute | Sample_ID | bcodmo_name | String | sample |
attribute | Sample_ID | description | String | Sample ID, unique sample identifier |
attribute | Sample_ID | long_name | String | Sample ID |
attribute | Sample_ID | nerc_identifier | String | https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ |
attribute | Sample_ID | units | String | unitless |
variable | Date | String | ||
attribute | Date | bcodmo_name | String | date |
attribute | Date | description | String | Date the samples were analyzed in ISO 8601 format yyyy-mm-dd |
attribute | Date | long_name | String | Date |
attribute | Date | nerc_identifier | String | https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/ |
attribute | Date | source_name | String | Date |
attribute | Date | time_precision | String | 1970-01-01 |
attribute | Date | units | String | nd |
variable | Analyte_Name | String | ||
attribute | Analyte_Name | bcodmo_name | String | sample_descrip |
attribute | Analyte_Name | description | String | Element analyzed |
attribute | Analyte_Name | long_name | String | Analyte Name |
attribute | Analyte_Name | units | String | unitless |
variable | Int_Corr | double | ||
attribute | Int_Corr | _FillValue | double | NaN |
attribute | Int_Corr | actual_range | double | -8736.84, 3.163645165E7 |
attribute | Int_Corr | bcodmo_name | String | unknown |
attribute | Int_Corr | description | String | Intensity (corrected) |
attribute | Int_Corr | long_name | String | Int Corr |
attribute | Int_Corr | units | String | unitless |
variable | RSD_Corr_Int | float | ||
attribute | RSD_Corr_Int | _FillValue | float | NaN |
attribute | RSD_Corr_Int | actual_range | float | 0.14, 1383.25 |
attribute | RSD_Corr_Int | bcodmo_name | String | unknown |
attribute | RSD_Corr_Int | description | String | Relative standard deviation (RSD) of corrected intensity |
attribute | RSD_Corr_Int | long_name | String | RSD Corr Int |
attribute | RSD_Corr_Int | units | String | unitless |
variable | Conc_Calib | float | ||
attribute | Conc_Calib | _FillValue | float | NaN |
attribute | Conc_Calib | actual_range | float | -0.2, 122.54 |
attribute | Conc_Calib | bcodmo_name | String | P |
attribute | Conc_Calib | description | String | Calibrated concentration of total phosphorous |
attribute | Conc_Calib | long_name | String | Conc Calib |
attribute | Conc_Calib | units | String | parts per million (ppm) |