BCO-DMO ERDDAP
Accessing BCO-DMO data
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Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.897359.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/897359 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL license String The data may be used and redistributed for free but is not intended\nfor legal use, since it may contain inaccuracies. Neither the data\nContributor, ERD, NOAA, nor the United States Government, nor any\nof their employees or contractors, makes any warranty, express or\nimplied, including warranties of merchantability and fitness for a\nparticular purpose, or assumes any legal liability for the accuracy,\ncompleteness, or usefulness, of this information.
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL summary String Dissolved organic phosphorus (DOP) hydrolysis rates from marine bacterium Ruegeria pomeroyi laboratory cultures.\n\nThese data were collected as part of a study of \"Dissolved organic phosphorus utilization by the marine bacterium Ruegeria pomeroyi DSS-3 reveals chain length-dependent polyphosphate degradation\" (Adams et al., 2022).\n\nStudy abstract:\nDissolved organic phosphorus (DOP) is a critical nutritional resource for marine microbial communities. However, the relative bioavailability of different types of DOP, such as phosphomonoesters (P-O-C) and phosphoanhydrides (P-O-P), is poorly understood. Here we assess the utilization of these P sources by a representative bacterial copiotroph, Ruegeria pomeroyi DSS-3. All DOP sources supported equivalent growth by R. pomeroyi, and all DOP hydrolysis rates were upregulated under phosphorus depletion (-P). A long-chain polyphosphate (45polyP) showed the lowest hydrolysis rate of all DOP substrates tested, including tripolyphosphate (3polyP). Yet the upregulation of 45polyP hydrolysis under -P was greater than any other substrate analyzed. Proteomics revealed three common P acquisition enzymes potentially involved in polyphosphate utilization, including two alkaline phosphatases, PhoD and PhoX, and one 5'-nucleotidase (5'-NT). Results from DOP substrate competition experiments show that these enzymes likely have broad substrate specificities, including chain length-dependent reactivity toward polyphosphate. These results confirm that DOP, including polyP, are bioavailable nutritional P sources for R. pomeroyi, and possibly other marine heterotrophic bacteria. Furthermore, the chain-length dependent mechanisms, rates and regulation of polyP hydrolysis suggest that these processes may influence the composition of DOP and the overall recycling of nutrients within marine dissolved organic matter.
attribute NC_GLOBAL title String [Ruegeria pomeroyi DOP hydrolysis rates] - Dissolved organic phosphorus (DOP) hydrolysis rates from Ruegeria pomeroyi laboratory cultures (Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle)
variable Media_Type String
attribute Media_Type long_name String Media_type
attribute Media_Type units String unitless
variable Growth_Phase String
attribute Growth_Phase long_name String Growth_phase
attribute Growth_Phase units String unitless
variable Sample_Type String
attribute Sample_Type long_name String Sample_type
attribute Sample_Type units String unitless
variable DOP_Substrate String
attribute DOP_Substrate long_name String Dop_substrate
attribute DOP_Substrate units String unitless
variable Hydrolysis_Rate float
attribute Hydrolysis_Rate actual_range float 0.0426, 233.72
attribute Hydrolysis_Rate long_name String Hydrolysis_rate
attribute Hydrolysis_Rate units String umol Pi L-1 hr-1

 
ERDDAP, Version 2.22
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