BCO-DMO ERDDAP
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Row Type | Variable Name | Attribute Name | Data Type | Value |
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attribute | NC_GLOBAL | cdm_data_type | String | Other |
attribute | NC_GLOBAL | Conventions | String | COARDS, CF-1.6, ACDD-1.3 |
attribute | NC_GLOBAL | creator_email | String | info at bco-dmo.org |
attribute | NC_GLOBAL | creator_name | String | BCO-DMO |
attribute | NC_GLOBAL | creator_url | String | https://www.bco-dmo.org/ |
attribute | NC_GLOBAL | doi | String | 10.26008/1912/bco-dmo.897359.1 |
attribute | NC_GLOBAL | infoUrl | String | https://www.bco-dmo.org/dataset/897359 |
attribute | NC_GLOBAL | institution | String | BCO-DMO |
attribute | NC_GLOBAL | license | String | The data may be used and redistributed for free but is not intended\nfor legal use, since it may contain inaccuracies. Neither the data\nContributor, ERD, NOAA, nor the United States Government, nor any\nof their employees or contractors, makes any warranty, express or\nimplied, including warranties of merchantability and fitness for a\nparticular purpose, or assumes any legal liability for the accuracy,\ncompleteness, or usefulness, of this information. |
attribute | NC_GLOBAL | sourceUrl | String | (local files) |
attribute | NC_GLOBAL | summary | String | Dissolved organic phosphorus (DOP) hydrolysis rates from marine bacterium Ruegeria pomeroyi laboratory cultures.\n\nThese data were collected as part of a study of \"Dissolved organic phosphorus utilization by the marine bacterium Ruegeria pomeroyi DSS-3 reveals chain length-dependent polyphosphate degradation\" (Adams et al., 2022).\n\nStudy abstract:\nDissolved organic phosphorus (DOP) is a critical nutritional resource for marine microbial communities. However, the relative bioavailability of different types of DOP, such as phosphomonoesters (P-O-C) and phosphoanhydrides (P-O-P), is poorly understood. Here we assess the utilization of these P sources by a representative bacterial copiotroph, Ruegeria pomeroyi DSS-3. All DOP sources supported equivalent growth by R. pomeroyi, and all DOP hydrolysis rates were upregulated under phosphorus depletion (-P). A long-chain polyphosphate (45polyP) showed the lowest hydrolysis rate of all DOP substrates tested, including tripolyphosphate (3polyP). Yet the upregulation of 45polyP hydrolysis under -P was greater than any other substrate analyzed. Proteomics revealed three common P acquisition enzymes potentially involved in polyphosphate utilization, including two alkaline phosphatases, PhoD and PhoX, and one 5'-nucleotidase (5'-NT). Results from DOP substrate competition experiments show that these enzymes likely have broad substrate specificities, including chain length-dependent reactivity toward polyphosphate. These results confirm that DOP, including polyP, are bioavailable nutritional P sources for R. pomeroyi, and possibly other marine heterotrophic bacteria. Furthermore, the chain-length dependent mechanisms, rates and regulation of polyP hydrolysis suggest that these processes may influence the composition of DOP and the overall recycling of nutrients within marine dissolved organic matter. |
attribute | NC_GLOBAL | title | String | [Ruegeria pomeroyi DOP hydrolysis rates] - Dissolved organic phosphorus (DOP) hydrolysis rates from Ruegeria pomeroyi laboratory cultures (Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle) |
variable | Media_Type | String | ||
attribute | Media_Type | long_name | String | Media_type |
attribute | Media_Type | units | String | unitless |
variable | Growth_Phase | String | ||
attribute | Growth_Phase | long_name | String | Growth_phase |
attribute | Growth_Phase | units | String | unitless |
variable | Sample_Type | String | ||
attribute | Sample_Type | long_name | String | Sample_type |
attribute | Sample_Type | units | String | unitless |
variable | DOP_Substrate | String | ||
attribute | DOP_Substrate | long_name | String | Dop_substrate |
attribute | DOP_Substrate | units | String | unitless |
variable | Hydrolysis_Rate | float | ||
attribute | Hydrolysis_Rate | actual_range | float | 0.0426, 233.72 |
attribute | Hydrolysis_Rate | long_name | String | Hydrolysis_rate |
attribute | Hydrolysis_Rate | units | String | umol Pi L-1 hr-1 |